• Korean Journal of Poultry Science
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• v.36 no.2
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• pp.177-186
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• 2009

It is well-known that unregulated over-expression of foreign gene may have unwanted physiological or toxic effects in transgenic animals. To circumvent these problems, we constructed retrovirus vector designed to express the foreign gene under the control of the tetracycline-inducible promoter. However, gene expressions in the tetracycline-inducible expression system (Tet system) are not completely regulated but a little leaky due to the inherent defects in conventional Tet-based systems. A more tightly controllable regulatory system can be achieved when the advanced versions ($rtTA2^SM2$) of rtTA and a minimal promoter in responsive components (pTRE-tight) are used in combination therein. In this study, we tried to produce human thrombopoietin (hTPO) from various target cells and transgenic chickens using the retrovirus vector combined with Tet system. hTPO is the primary regulator of platelet production and has an important role in the survival and expansion of hematopoietic stem cells. In a preliminary experiment in vitro, higher hTPO expression and tighter expression control were observed in chicken embryonic fibroblast (CEF) cells. We also measured the biological activity of the hTPO using Mo7e cells whose proliferation is dependant on hTPO. The biological activity of the recombinant hTPO from CEF was higher than both its commercial counterpart and hTPO from other target cells. The recombinant retrovirus was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 138 injected eggs, 15 chicks hatched after 21 days of incubation. Among them, 8 hatched chicks were hTPO positive. When the Go transgenic chicken was fed doxycycline (0.5 mg per 1 gram of feed), a tetracycline derivative, hTPO concentration of the transgenic chicken blood was 200 ng/mL. Germline transmission of the transgene was confirmed in sperm of the Go transgenic roosters. These results are informative to establish transgenic chickens as bioreactors for the mass production of commercially valuable and biological active human cytokine proteins.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.9
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• pp.846-852
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• 2013

In this paper, the authors propose a new approach to control problem of the marine vessels which are moored or controlled by actuators. The vessel control problem in the specified area is called a DPS (Dynamic Positioning System). The main objective of this paper is to obtain more useful control design method for DPS. In this problem, a complicate fact is control allocation which is a numerical method for distributing the control signal to the controlled system. For this, many results have been given and verified by other researchers using two individual processes. It means that the controller design and control allocation design process are carried out individually. In this paper, the authors give more sophisticated design solution on this issue. In which the controller design and control allocation problem are unified by a robust controller design problem. In other word, the stability of the closed-loop system, control performance and allocation problem are unified by an LMI (Linear Matrix Inequality) constraint based on $H_2/H_{\infty}$ mixed design framework. The usefulness of proposed approach is verified by simulation with a supply vessel model and found works well.

• Journal of Industrial Technology
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• v.29 no.A
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• pp.83-88
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• 2009

The removal efficiency of organic compounds and the toxicity evaluation of microorganism have been studied in dye wastewater treatment using $UV/TiO_2$ and $UV/H_2O_2$ photooxidation system. Sample waters tested in this work were raw dye wastewater and dye wastewater treated in $UV/TiO_2$ and $UV/H_2O_2$ photooxidation system respectively. Total organic carbon(TOC) removal rate was 50% in $UV/TiO_2$ process and 80% in $UV/H_2O_2$ process. It has been investigated with colony counting agar method and paper disk method whether the type of treatment process has affected the microorganism growth. In the raw wastewater, more than four types of microorganisms have survived. But, little of microorganisms were alive at TOC removal rate of 50% in $UV/TiO_2$ system. In contrast to that, two types of microorganisms were found at TOC removal rate of 80% in $UV/H_2O_2$ system.

• Proceedings of the KIPE Conference
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• 2003.07b
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• pp.584-587
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• 2003

Iterative learning control is an approach to improve the transient response of systems that operate repetitively over a fixed time interval. It is useful for the system where the system output follows the different type input, in case of design or modeling uncertainty In this paper, we introduce the concept of iterative learning control and then apply the learning control algorithm for multi-motor system for performance Improvement.

• Proceedings of the KIPE Conference
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• 2003.07b
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• pp.769-772
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• 2003

In this paper are described design of hardware and GUI(Graphical User Interface) for a PC based Weighting System. Conventional Weighting System is adapted microprocessor system for measuring and controlling. This system should have big memory for the management of measured data and is difficult to operate. For such reason a new Weighting System based on PC is proposed. In this contribution is handled these problems.

• Analytical Science and Technology
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• v.7 no.3
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• pp.339-347
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• 1994

A multicolumn system consisted of two valve(10-port and 6-port valve)-three column (28% DC 200, SP 1700 and Chromosorb 102 column) was developed. Nine natural gas components composed of $N_2$, $CH_4$, $CO_2$, $C_2H_6$, $C_3H_8$, $i-C_4H_{10}$, $n-C_4H_{10}$, $i-C_5H_{12}$ 및 $n-C_5H_{12}$ completed all the baseline separation within 18 minutes. The accuracy and the precision of this system was tested. The retention times and the peak areas were determined with a repeatability between 0.02 and 0.16%, and less than 1%, respectively. Calibration curves for natural-gas components were plotted by the partial pressure injection method of pure gases, and good linear relationships for each component were presented. By using these calibration curves the accuracy of the multicolumn system compaired with that of the single column system for a certified standard gas of natural gas. As a result, relative error in the single and the multicolumn system was less than 0.5% and 0.04%, respectively. The result of application of this system in the analysis of importing LNG composition showed that the heating values calculated by the multicolum system were estimated lower compared with those calculated by the single column system and consequently, the importing price of LNG was able to be cut down.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.10a
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• pp.219-220
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• 2009

• Proceedings of the KIEE Conference
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• 1997.07f
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• pp.2013-2015
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• 1997

Elevator stops emergently when power failures happen during operation. In that case, the passenger should wait with frightened in the car untill they are rescued by repair man or power resumes. This paper describes an automatic landing device for power-failure(ALP) that consists of UPS and battery charge control system. The excellence of the ALP operation is verified by experiments in elevator test tower.

• Journal of Power System Engineering
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• v.11 no.4
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• pp.50-55
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• 2007

Nowadays, demands on super low temperature condition for industrial and commercial uses are thriving. Considering of its wide application in the present and the future, study of the super-low temperature refrigeration system should be actively carried out. This study is aimed to investigate refrigeration capacity and coefficient of performance(COP) of the cascade refrigeration system, as well as to get the system which can reach evaporator temperature of $-70^{\circ}C$. For this purpose, R290 and R170 are charged in high stage and low stage respectively. Finally the characteristics of system using R290 and R170 will be proposed. Additionally, In this experiment, the flow rate of air flowing through the LS evaporator and the compressor inlet pressure were varied to investigate the refrigeration capacity and coefficient of performance characteristics.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.633-634
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• 2006

Recently we started a development of the digital meister expert system for the product design supporting in manufacturing industry. Knowledge representation is of major importance in digital moister expert system. This rule-based expert system-knowledges are designed for a certain type of knowledge representation such as rules or logic. The way in which a rule-based expert system represents knowledge affects the development, efficiency, speed, and maintenance. Eventually, this digital moister system is used to the engineer in manufacturing industry for the process control, production management and system management. In this paper, we propose the digital moister system knowledge representation method for product design supporting in manufacturing industry and we present introduction and contents of rule-based knowledge representation supporting tool.