• Microbiology and Biotechnology Letters
• /
• v.14 no.6
• /
• pp.441-446
• /
• 1986

Some properties of extracellular guanine deaminase produced by Pseudomonas synxantha A3 were studied. The enzyme was stable at pH 6.5-7.5 and generally stable when it was incubated at 4$0^{\circ}C$ for 10 minutes but inactivated gradually above 4$0^{\circ}C$. When the enzyme in 0.2M potassium phosphate (pH 8.0) was stored at room temperature, it was stable for thirty days. Alcohols and acetone were not effective for the eyzyme stability. The optimum pH and temperature for the enzyme activity were around pH 7.0-8.0 and 5$0^{\circ}C$, respectively. The enzyme was inhibited by 1mM of Hg$^{++}$, Ag$^+$ and Li$^+$ and by 0.1mM of Ag$^+$ with about 50% loss of activity. The enzyme inhibited by Li$^+$ was reactivated by EDTA. 1 mM of pentachlorophenol and p-CMB inactivated the enzyme with 50% and 40% loss of activity, respectively. The enzyme inactivated by p-CMB was reactivated by glutathione.

• Microbiology and Biotechnology Letters
• /
• v.19 no.4
• /
• pp.383-389
• /
• 1991

In order to produce alkaline protease, psychrotrophic bacterium which have high enzyme activity at low temperature, was isolated by using enrichment culture from various samples and identified as genus alkalopsychrotropic Pseudomonas sp. RP-222. The optimal culture conditions for enzyme production were pH- 10.0, temperature-$20^{\circ}C$ and culture time-4 days. The optimum pH and temperature for the enzyme activity were pH 10.5 and $40^{\circ}C$, respectively and the enzyme was relatively stable at pH 7.0~13.0 and below $50^{\circ}C$. The enzyme was inhibited by ethylenediaminetetraacetate and phenylmethylsulfonylfluoride, indicating that the enzyme was a serine metalloenzyme, but considerably stable in the presence of surface active agents. Activity of the enzyme was increased by the addition of 0.05% Na-$\alpha$-olefin sulfonate.

• Journal of applied mathematics & informatics
• /
• v.30 no.3_4
• /
• pp.511-516
• /
• 2012

In this paper, we investigate $h$-stability of the nonlinear perturbed differential systems using the the notion of $t_{\infty}$-similarity.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2004.08a
• /
• pp.265-268
• /
• 2004

We have developed a highly efficient and stable blue organic electroluminescent device (OLED) based on the blue fluorescent p-bis(p-N,N-diphenyl-aminostyryl)benzene (DSA-Ph) dopant in a morphologically stable high-bandgap host material, 2-methyl-9,10-di(2-naphthyl)anthracene (MADN), which achieved an EL efficiency of 9.7 cd/A and 5.5 lm/W at 20 mA/$cm^2$ and 5.7 V with a Commission Internationale d'Eclairage coordinates of(x = 0.16, y = 0.32). This sky blue device which could also alleviate the problematic current induced quenching at high current achieved a half-decay lifetime ($t_{1\;2}$) of 46,000 h at an initial brightness of 100 cd/$m^2$.

• Journal of Environmental Health Sciences
• /
• v.16 no.2
• /
• pp.89-95
• /
• 1990

This study was investigated to the energy recovery by the pyrolysis of waste tyre. the pyrolysis of the waste tyre was made by using the pyrolysis chamber for the gasification and the combustion chamber for the combustion of the pyrolysis gas. In batch system, the amount of waste tyre was put 150kg in the pyrolysis chamber and the proper air flow rate for the stable production of the pyrolysis gas was 0.95Nm$^{3}$ /min. the production time of the pyrolysis gas was stable above 210minutes, and the stable production rate was above 3.8Nm$^{3}$ /min. The production temperature of pyrolysis gas was 170$^{\circ}$C and combustion temperature of pyrolysis gas was 1,000$^{\circ}$C. The combustible component of washing gas in pyrolysis gas of waste tyre was CO, CH$_{4}$, $C_{2}H_{6}$ and $C_{3}H_{8}$, and total amount was 22.7%. Heat value of condensed material was 9,804Kcal/kg. The average concentration of air pollutants between cyclone and scrubber was CO 420.4ppm, SO$_{x}$ 349.8ppm. NO$_{x}$ 68.Sppm, HCl 24.4ppm and Dust 240.0g / Nm$^{3}$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.28 no.6
• /
• pp.728-735
• /
• 1995

The peptidyl prolyl cis-trans isomerase(PPlase, EC 5.2.2.8) from Bacillus stearothermophilus SIC1 was extracted from the cells treated with by lysozyme. PPlase was purified from the cell extracts by heat treatment, ammonium sulfate precipitation, ion exchange chromatography and finally gel filtration (FPLC). The purity of purified the enzyme after Superose 12 column chromatography was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). The molecular weight of the purified PPlase was estimated as 18,000 by SDS-PAGE. The 39 amino acid residues from the N-terminus were determined by the protein sequencer. The enzyme showed the optimum pH at 8.0 and was stable at the range of pH 7.0 to 8.0. The enzyme was considerably stable after heat treatment at $60^{\circ}C$ for 30 minutes, and the enzyme was quite stable up to $65^{\circ}C$. The presence of the PPlase in the refolding solution accelerated the isomerization rate of the assay peptide.

• Proceedings of the KIEE Conference
• /
• 2002.07c
• /
• pp.1853-1855
• /
• 2002

A pulsed Nd:YAG laser is used widely in many applications such as materials processing, communications, military and medical instruments. It is important to control the laser energy in those fields with using a pulsed Nd:YAG laser. In this paper, constant-frequency current resonant half-bridge inverter and feedback control circuit are used to have output stable. This laser power supply is designed and fabri which was isolated. Also, this study is used an one-chip microprocessor, SCR, current transform A stable output of this Nd:YAG laser system investigated. The tested results are described.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.20 no.6
• /
• pp.621-628
• /
• 1991

Heat-stable enterotoxin(ST) from enterotoxigenic E. coli eKT-53($ST^{+}\;LT^{-}$, transformant from isolate KM-7) that was produced in succinate salts medium. The culture supernatant(crude ST) was purifed by mulitpled steps and investigated some characterization of the ST. The heatstability of purified ST activity was completely lost by treating at $100^{\circ}C$ for 30minutes. ST activity was lost by treatment at pH 1 and 12 conditions, while the activity was not reduced by treatment at pH 2~10, and then the ${\alpha}-amylase$ and pepsin was not decreased activity but disulfide reducing agnets was lost the activity. The molecular weight of the purified ST was approximately 4,200, the isoelectric point was about 4.0.

• Journal of the Chungcheong Mathematical Society
• /
• v.13 no.1
• /
• pp.45-51
• /
• 2000

We will investigate some properties of integro-delay-differential equations, $$x^{\prime}(t)=A(t)x(t-g_1(t,x_t))+{\int}_{t_0}^{t}B(t,s)x(s-g_2(s,x_s))ds,\;t_0{\geq}0,\\x(t_0)={\phi}$$,

• Journal of the Korean Mathematical Society
• /
• v.43 no.4
• /
• pp.783-801
• /
• 2006

Let A be the mod p Steenrod algebra for p an arbitrary odd prime and S the sphere spectrum localized at p. In this paper, some useful propositions about the May spectral sequence are first given, and then, two new nontrivial homotopy elements ${\alpha}_1{\jmath}{\xi}_n\;(p{\geq}5,n\;{\geq}\;3)\;and\;{\gamma}_s{\alpha}_1{\jmath}{\xi}_n\;(p\;{\geq}\;7,\;n\;{\geq}\;4)$ are detected in the stable homotopy groups of spheres, where ${\xi}_n\;{\in}\;{\pi}_{p^nq+pq-2}M$ is obtained in [2]. The new ones are of degree 2(p - 1)($p^n+p+1$) - 4 and 2(p - 1)($p^n+sp^2$ + sp + (s - 1)) - 7 and are represented up to nonzero scalar by $b_0h_0h_n,\;b_0h_0h_n\tilde{\gamma}_s\;{\neq}\;0\;{\in}\;Ext^{*,*}_A^(Z_p,\;Z_p)$ in the Adams spectral sequence respectively, where $3\;{\leq}\;s\;<\;p-2$.