• 미생물학회지
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• 제52권4호
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• pp.437-443
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• 2016

여러 토양에서 분리한 400여 개의 방선균 균주에 대해 4가지 식물병원성 진균에 대한 항진균 활성을 조사하였으며 그 중 Streptomyces costaricanus HR391 균주는 PDB 배지에서 식물병원성 진균인 Fusarium oxysporum f. sp. raphani, F. oxysporum f. sp. niveum, F. oxysporum f. sp. lycopersici와 Rhizoctonia solani의 균사 생장을대조군과 비교하여 각각 26.5, 26.2, 21.2와 23.8% 저해하였다. S. costaricanus HR391은 항진균물질인 siderophore를 $98{\mu}M$을 생성할 뿐만 아니라 유화활성을 나타내며 막지질을 파괴할 수 있는 생물계면활성제인 rhamnolipid와 lipopeptide인 iturin A와 surfactin를 생성하였다. 또한 진균 세포막을 분해할 수 있는 chitinase와 glucanase 활성도 나타내었으며 병원균의 막을 파괴하는 AMP와 항생물질 phenazine도 분비하였다. 이외에도 식물생장 촉진활성을 갖는 zeatin, gibberellin과 indole acetic acid 같은 식물호르몬도 생성하였다. 이와 같이 항진균 활성을 나타내는 S. costaricanus HR391 균주는 다양한 종류의 항진균 물질의 상승작용과 더불어 높은 생물계면활성이 본 균주의 항진균 활성에 큰 역할을 하는 것으로 보이며 친환경적인 생물학적 항진균제로서 활용이 가능할 것으로 기대된다.

• Journal of Periodontal and Implant Science
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• 제29권2호
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• pp.355-373
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• 1999

Many researches have been reported that collagen as cellular stroma, matrix of grafting materials, mediator of agents for the purpose of promoting healing process invivo, but the responses in vivo were seen various. The goal of this experiment is to assess the effect of collagen on bony healing, through histological evaluation of implanted collagen on the calvarial defect in rats. 2-month-old Sprague-Dawley, 24 rats were used and 12 rats assigned to each group of control and test. Defect of 5mm in diameter was made on the calvarial bone with trephine bur. Following thorough saline rinse, defect of control group was left in empty and that of experimental group was filled with fibrillar collagen($COLLATAPE^{(R)}$, COLLA-TEC. INC. U.S.A.) soaked in saline. 3 rats in each group were sacrificed at 3, 7, 14, 21 days after operation respectively, and the tissue blocks were prepared for light microscope with H-E for evaluation of overall healing, with TRAP(tartrate resistant acid phosphatase) for evaluation of osteoclastic activity and with immunohistochemical staining for macrophages. The results were as follows : 1. In the control group, inflammatory responses were disappeared at day 14, but, in the experimental group inflammatory infiltrates were reduced at day 21. Thus, the experimental group showed more severe soft tissue inflammation than control group. 2. Both control and experimental group showed slight appositional growth at day 7 and gradual bony growth to 21th day. But, complete bony healing of the defect was not shown. There was no significant difference in bony healing between control and experimental group 3. Specific response of macrophages for implanted collagen was observed at day 14 in the experimental group. In conclusion, although fibrillar collagen caused inflammation of soft tissue during initial healing period, inflammatory responses by fibrillar collagen didn't inhibit bony regeneration and implanted collagen was biodegradaded by macrophages. Thus, we expect that fibrillar collagen can be used for useful mediator of graft materials or growth factors.

• Journal of Acupuncture Research
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• 제33권1호
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• pp.47-56
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• 2016

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

• 생명과학회지
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• 제13권5호
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• pp.582-589
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• 2003

본 연구에서는 가지과 작물의 근권에서 유용한 균주를 분리하고 근권ㆍ길항ㆍ생육촉진미생물을 선별ㆍ제제화하고 상토에 첨가하여 미생물제제 혼입 상토를 제조한 후 고추 플러그묘의 초기 생장에 미치는 영향을 조사하였다. 근권미생물로는 가지과 작물의 뿌리 및 재배 토양 등 385점의 균원시료로부터 540여점을 분리한 후, 가지과 작물에 공통적으로 발생되는 Alternaria solani, Botrytis cinerea, Fusarium oxysporium, Phytophthora capsici, Sclerotinia sclerotiorum 등의 병원균에 높은 길항력을 가진 미생물 20여종을 선발하고, 이들 길항미생물 중에서 고추의 초기 생육을 촉진시키는 MJ-3균주를 최종 선발하였다. 근권ㆍ길항ㆍ생육촉진미생물인 분리주 MJ-3은 Bergey's manual of systematic bacteriology의 방법과 16S rDNA sequence법 등으로 조사하여 Bacillus amyloliquefaciens동정하였다. 제제화한 B. amyloliquefaciens를 상토에 첨하여 미생물 혼입 상토를 제조하였고, 이를 이용하여 재배한 고추 플러그 묘는 초장과 경경, 뿌리길이, 생체중량 등이 모두 대조구에 비해 소량 증가되었으나, 특히 뿌리의 생체중량이 대조구가 0.61g/plant인데 비해 시험구는 0.88g/plant로 44.3% 증가하였다. 미생물제제 첨가 상토에서 재배한 고추의 gib-berellin (GA$_1$) 함량은 0.559ng/g(건조중량)이었다.

• Molecules and Cells
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• 제44권11호
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• pp.830-842
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• 2021

When perceiving microbe-associated molecular patterns (MAMPs) or plant-derived damage-associated molecular patterns (DAMPs), plants alter their root growth and development by displaying a reduction in the root length and the formation of root hairs and lateral roots. The exogenous application of a MAMP peptide, flg22, was shown to affect root growth by suppressing meristem activity. In addition to MAMPs, the DAMP peptide PEP1 suppresses root growth while also promoting root hair formation. However, the question of whether and how these elicitor peptides affect the development of the vascular system in the root has not been explored. The cellular receptors of PEP1, PEPR1 and PEPR2 are highly expressed in the root vascular system, while the receptors of flg22 (FLS2) and elf18 (EFR) are not. Consistent with the expression patterns of PEP1 receptors, we found that exogenously applied PEP1 has a strong impact on the division of stele cells, leading to a reduction of these cells. We also observed the alteration in the number and organization of cells that differentiate into xylem vessels. These PEP1-mediated developmental changes appear to be linked to the blockage of symplastic connections triggered by PEP1. PEP1 dramatically disrupts the symplastic movement of free green fluorescence protein (GFP) from phloem sieve elements to neighboring cells in the root meristem, leading to the deposition of a high level of callose between cells. Taken together, our first survey of PEP1-mediated vascular tissue development provides new insights into the PEP1 function as a regulator of cellular reprogramming in the Arabidopsis root vascular system.

• 한국축산식품학회지
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• 제35권2호
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• pp.211-215
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• 2015

From the previous study, Enterococcus faecium SH01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin SH01. Bacteriocin SH01 showed an inhibitory effect against Listeria monocytogenes ATCC 19111, a bacterial strain causing human listeriosis. Crude bacteriocin SH01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 AU/g) against Listeria monocytogenes ATCC 19111 was investigated in ground beef at increasing temperatures (5, 10, 15, and 20℃) for 8 d. The number of Listeria monocytogenes ATCC 19111 significantly decreased (p<0.05) as the concentration of bacteriocin increased from 500 to 1,000 AU/g. Intrinsic crude protease activities in ground beef were examined and increased as the temperature increased. Experiments varying both the concentrations of added bacteriocin SH01 and temperature demonstrated a maximum inhibition (2.33 log reduction of bacteria) in samples containing 1,000 AU/g of bacteriocin SH01 incubated at 20℃. When the crude bacteriocin SH01 solution (1,280 AU/mL) was incubated with crude protease solutions at different temperatures, its activity decreased by only half (640 AU/mL), as assessed in an agar well diffusion assay. The finding that the antilisterial activity of bacteriocin SH01 increased with temperature can be explained by the fact that higher temperatures increase bacterial membrane fluidity, thereby promoting the cellular penetration of bacteriocin SH01 into L. monocytogenes. Bacteriocin SH01 may be an excellent candidate as a biopreservative for controlling L. monocytogenes growth in ground beef.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2002년도 창립 10주년 기념 국제학술심포지움
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• pp.64-71
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• 2002

Rice(Oryza stiva L.) is a major cereal food providing nourishment to over half of the world's populations and was considered only as a source of energy. However, The recent many researches have been made to suggest that rice may relate to prevention chronic disease and health-promoting properties such as postprandial glucose response, hypocholesterolemic effect and blood pressure-lowering effect. There has been numerous observation supporting that rice has hypocholesterolemic effect. Rice, rice bran, rice bran oil and unsaponifiable matter of rice bran oil reduced plasma cholesterol in rat, hamster as well as human. Components of rice showing hypocholesterolemic effect include dietary fiber(hemicellulose, phytic acid). protein, ${\gamma}$-oryzanol, $\beta$-sitosterol, and tocotrienols. Crapo et al has been studied that the effect of various of starchy foods on the postprandial blood glucose and insulin responses in healthy and diabetic humans. The results showed that rice had lower blood glucose and insulin responses compared to potato, bread and dextrose. The different physical forms in the same starch also produce the different postprandial glucose and insulin responses. In recent years, several studies have shown that some components of rice have potent antioxidant activity against Fe$^{2+}$ -ascorbate induced lipid peroxidation in rat liver microsomal membranes. Cell culture and animal studies have shown that some components of rice have inhibitory effect on the growth and proliferation of several types of human cancer cell. It was also reported that the methanol extract of brown rice has antimutagenic activity against various mutagens. In addition, the pepsine hydrolysate from rice protein is reported to inhibit angiotensin converting enzyme activity. GABA (${\gamma}$ - aminobutyric acid) and GABA enriched rice germ is also effective for lowering blood pressure and triglyceride levels.s.

• 대한약침학회지
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• 제18권4호
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• pp.20-25
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• 2015

Objectives: Recently, thread-embedding therapy (TET) has been widely applied in Korean medicine for cosmetic purposes such as reducing skin wrinkles. An inserted thread was reported to have induced continuous stimulation, followed by support for connective tissue regeneration. However, the potential role of TET in hair-growth has not yet been reported. Methods: We designed this study to evaluate whether TET has a hair-growth-promoting effect. C57 black 6 (C57BL/6) mice were divided into three groups: normal saline-treated, minoxidil-treated, and thread-embedded groups. Normal saline or 5% minoxidil was topically sprayed on the dorsal skin of the mice once a day for 16 days. Medical threads were embedded into the dorsal skin of the mice in a single application. Hair growth activity was evaluated by using dermoscopic and microscopic observations. Sections of the dorsal skin were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), fibroblast growth factor-7 (FGF-7), and fibroblast growth factor-5 (FGF-5) were detected by using immunohistochemical staining. A reverse transcription-polymerase chain reaction (RT-PCR) analysis was adopted to measure the messenger RNA (mRNA) expressions of FGF-7 and FGF-5. Results: TET enhanced anagen development in the hair follicles of C57BL/6 mice. The expressions of BrdU and PCNA, both of which imply active cellular proliferation, were increased by using TET. Moreover, TET increased the expression of FGF-7, an anagen-inducing growth factor, while decreasing the expression of FGF-5, an anagen-cessation growth factor, both at the protein and the mRNA levels. Conclusion: TET enhanced hair re-growth in C57BL/6 mice. TET regulated the expressions of anagen-associated growth factors and activated the proliferation of hair follicular cells in depilated skin lesions. Considering its long-lasting effect, TET may be a good alternative therapeutic for the treatment of alopecia.

• Journal of Ginseng Research
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• 제44권1호
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• pp.168-177
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• 2020

Background: Ginseng has been widely used as a health-promoting tonic. Gintonin present in ginseng acts as a lysophosphatidic acid (LPA) receptor ligand that activates six LPA receptor subtypes. The LPA6 subtype plays a key role in normal hair growth, and mutations in the LPA6 receptor impair normal human hair growth. Currently, human hair loss and alopecia are concerning issues that affect peoples' social and day-to-day lives. Objective: We investigated the in vitro and in vivo effects of a gintonin-enriched fraction (GEF) on mouse hair growth. Methods: Human hair follicle dermal papilla cells (HFDPCs) and six-week-old male C57BL/6 mice were used. The mice were divided into the four groups: control, 1% minoxidil, 0.75% GEF, and 1.5% GEF. The dorsal hair was removed to synchronize the telogen phase. Each group was treated topically, once a day, for 15 days. We analyzed hair growth activity and histological changes. Results: GEF induced transient [Ca²⁺]_i, which stimulated HFDPC proliferation and caused 5-bromo-2'-deoxyuridine (BrdU) incorporation in a concentration-dependent manner. GEF-mediated HFDPC proliferation was blocked by the LPA receptor antagonist and Ca²⁺ chelator. HFDPC treatment with GEF stimulated vascular endothelial growth factor release. Topical application of GEF and minoxidil promoted hair growth in a dose-dependent manner. Histological analysis showed that GEF and minoxidil increased the number of hair follicles and hair weight. Conclusion: Topical application of GEF promotes mouse hair growth through HFDPC proliferation. GEF could be one of the main components of ginseng that promote hair growth and could be used to treat human alopecia.

• 생명과학회지
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• 제34권5호
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• pp.304-312
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• 2024

가금류 우모는 환경오염 물질이자 병원성 세균의 저장소로 간주되는 축산폐기물이다. 따라서 우모 폐기물을 관리하기 위한 지속 가능하고, 환경친화적인 방법을 개발하는 것은 매우 중요하다. 본 연구는 폐기된 닭의 우모로부터 분리된 Y10 균주를 동정하고, 특성화하기 위하여 수행되었다. Y10 균주는 표현형 및 16S rRNA 유전자 분석을 통해 Bacillus amyloliquefaciens에 속하는 것으로 확인되었다. B. amyloliquefaciens Y10은 곰팡이 세포성분을 분해하는 효소(cellulase, lipase, protease and pectinase), siderophore, 암모니아 및 IAA 생성과 같은 식물 성장 촉진 활성을 나타내었다. 나아가 Y10 균주는 일부 식물병원성 곰팡이의 균사체 생육을 억제할 수 있었다. 기본배지에 탄소원으로 sucrose 0.1%, 질소원으로 casein 0.05%를 첨가한 후, 배지의 pH를 10으로 조정하여 35℃에서 배양했을 때, 실험균주에 의한 우모 분해율은 기본배지 대비 약 2배 증가되었으며, 배양 4일에 우모는 완전히 분해되었다. 또한 실험균주는 개선된 조건에서 오리 우모, 양모, 사람의 손발톱과 같은 다양한 케라틴 기질을 분해할 수 있었다. Y3 균주를 이용하여 조제된 우모 분해산물은 DPPH 라디칼 소거능(EC₅₀ = 0.38 mg/ml)과 SOD 유사활성(EC₅₀ = 183.7 mg/ml)과 같은 항산화능이 있음을 확인하였다. 이 결과는 실험균주는 케라틴 폐기물의 미생물학적 처리뿐만 아니라 농축산 산업에 적용할 수 있는 생물 비료, 생물 농약 및 사료첨가제 개발의 잠재적인 후보가 될 수 있을 시사한다.