• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.149-149
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• 2017

The RING (Really Interesting New Gene) finger proteins are known to play crucial roles in various abiotic stresses in plants. In this study, we report on RING finger E3 ligase, ${\underline{O}ryza}$ ${\underline{s}ativa}$ ${\underline{s}alt$-, ${\underline{A}BA}$- and ${\underline{d}rounght}$ stress-${\underline{i}nduced}$ RING finger ${\underline{p}}rotein{\underline{1}}$ gene (OsSAD1). In vitro ubiquitination assay demonstrated that unlike OsSAD1, a single amino acid substitution ($OsSAD1^{C168A}$) of the RING domain showed no E3 ligase activity, supporting the notion that the activity of most E3s is specified by a RING domain. Result of Yeast-Two hybridization, In vivo protein degradation assay supports that OsSAD1 interacting with 3 substrate, OsSNAC2, OsGRAS44 and OsPIRIN1, and mediates proteolysis of 3 substrates via the 26S proteasome pathway. Subcellular localizations of OsSAD1 while approximately 62% of transient signals were detected in cytosol, 38% of signals were showed nucleus. However, transiently expression of OsSAD1 was detected in cytosol 30% while as 70% of nucleus under 200 mM salt treated rice protoplasts. Results of bimolecular fluorescence complementation (BiFC) showed that two nucleus-localized proteins (OsSNAC2 and OsGRAS44) interacted with OsSAD1 in the both cytosol and nucleus. Heterogeneous overexpression of OsSAD1 Heterogeneous overexpresssion of OsSAD1 in Arabidopsis exhibited sensitive phenotypes with respect to Salt-, mannitol-responsive seed germination, seedling growth. In ABA conditions, OsSAD1 overexpression plants showed highly tolerance phenotypes, such as root length and stomatal closure. Our findings suggest that the OsSAD1 may play a negative regulator in salt stress response by modulating levels of its target proteins.

• Journal of Astronomy and Space Sciences
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• 제39권2호
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• pp.67-77
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• 2022

The elements that impact the dynamics and collaborations of waves and particles in the magnetosphere of planets have been considered here. Saturn's internal magnetosphere is determined by substantiated instabilities and discovered to be an exceptional zone of wave activity. Interchanged instability is found to be one of the responsible events in view of temperature anisotropy and energization processes of magnetospheric species. The generated active ions alongside electrons that constitute the populations of highly magnetized planets like Saturn's ring electron current are taken into consideration in the current framework. The previous and similar method of characteristics and the perturbed distribution function have been used to derive dispersion relation. In incorporating this investigation, the characteristics of electromagnetic ion cyclotron wave (EMIC) waves are determined by the composition of ions in plasmas through which the waves propagate. The effect of ring distribution illustrates non-monotonous description on growth rate (GR) depending upon plasma parameters picked out. Observations made by Cassini found appropriate for modern study, have been applied to the Kronian magnetosphere. Using Maxwellian ring distribution function of ions and detailed mathematical formulation, an expression for dispersion relation as well as GR and real frequency (RF) are evaluated. Analysis of plasma parameters shows that, proliferating EMIC waves are not developed much when propagation is parallelly aligned with magnetosphere as compared to waves propagating in oblique direction. GR for the oblique case, is influenced by temperature anisotropy as well as by alternating current (AC) frequency, whereas it is much affected only by AC frequency for parallel propagating waves.

• 한국환경생태학회지
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• 제16권1호
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• pp.1-9
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• 2002

본 연구는 광양만 임해매립지의 곰솔 식재 이후 연륜생장에 영향을 미치는 식재환경과 기후요인을 조사 분석하였다. 임해매립지의 곰솔 식재 이후에 수목의 이식과 가뭄에 의하여 연륜생장이 급감소하였다. 수목이식에 의한 연륜생장의 급감소는 모든 지반들에서 유사하게 나타났다. 가뭄에 의한 연륜생장의 감소는 토양경도가 높은 식재지반에서 급격하게 감소하였으나 성토지역에서는 감소 현상이 크지 않았다. 이식에 의한 연륜생장 급감소 이후에 토양경도가 높은 지반들에서는 생장회복이 저조하였으나 성토지역에서는 빠르게 생장이 회복되었다. 연륜생장의 평균민감도와 상대적 변이계수는 토양경도가 높은 지반들이 성토지반들보다 높게 나타났다. 이것은 토양환경이 불량한 지반일수록 외부적 환경변화에 대하여 불안정적으로 생장하는 것으로 해석되었다. 이상의 결과를 요약하면 곰솔의 연륜생장은 이식과 가뭄에 대하여 성토지반에서는 수목 자체적으로도 양호하게 생장을 하지만 토양경도가 높은 지역에서는 생장이 불량하였다. 따라서. 외부적 환경변화에도 수목자체적으로 적응할 수 있는 식재 및 유지관리 방법이 모색되어져야 할 것으로 사료되었다.

• 한국기후변화학회지
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• 제7권4호
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• pp.465-476
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• 2016

This study was conducted to elucidate the ecological characteristics of sub-alpine coniferous forest on Banyabong, Mt. Jiri. Soil pH was 4.20. The contents of organic matter, total N, available P and CEC were 14.15%, 0.43%, 10.1 ppm and $17.17cmol^+/kg$, respectively. Sub-alpine coniferous forest had a relatively soil moisture content with 22.9%. According to the results of importance value analysis, Picea jezoensis (42.7) and Abies koreana (34.0) for tree layer, Rhododendron schlippenbachii (25.5) for subtree layer and Tripterygium regelii (18.4) and Rhododendron mucronulatum (16.6) for shrub layer were high, respectively. Shannon's diversity ranged from 0.3560 to 1.050. Evenness and dominance ranged from 0.398 to 0.733 and 0.267 to 0.601, respectively. A. koreana population had a reverse J-shaped DBH distribution, while P. jezoensis population had a normal DBH distribution. Height growth according to DBH of A. koreana was higher than that of P. jezoensis, which indicate than A. koreana have more effective water use efficiency than P. jezoensis. Annual mean tree ring growth of P. jezoensis and A. koreana showed up 1.792, 1.652, 1.389 mm/yr and 2.081, 1.921, 1.576 mm/yr for small, middle and large diameter tree, respectively.

• 동의생리병리학회지
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• 제21권1호
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• pp.82-85
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• 2007

The Chinese medicinal plant Artemisia annua is the source of the antimalarial compound artemisinin. By the way, Artemisin annula was known have endoperoxide ring structure is included and has anti-malarial effect. Malaria and Toxoplasma gondii (T. gondii) is belong to Apicomplexa genera. So, confirmed whether we go compound 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane that have endoperoxide ring structure and there is anti-toxoplasmosis effect. The efficacy of 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2] nonane alone was examined in vitro and in a murine model of acute toxoplasmosis. In vitro studies were peformed with HeLa cell cultures, with quantification of Toxoplasma growth by a cell proliferation assay. Selectivity of 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane was 4.9 in vitro cell proliferation assay, this is higher than sulfadiazine (selectivity was 1.63). For in vivo studies, mice were acutely infected intraperitoneally with 10$^5$ tachyzoites of the virulent RH strain and then treated perorally for 4 days from 6 hours postinfection. Efficacy was assessed by sequential determination of parasite burdens in peritoneal cavity. in vitro, 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane inhibited Toxoplasma growth at a concentration of 150mg/kg of body weight per day, the inhibition ratio was estimated to be 85.72%.

• 한국산학기술학회논문지
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• 제8권5호
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• pp.1201-1210
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• 2007

도시의 가로공간이 시민들에게 중요하고 이용하기 좋은 공공공간으로 역할하기 위해서는 가로계획이 단지 도시의 일부를 보행자가 이용하기 편리하고 쾌적함을 줄 수 있도록 계획하는 것에서 끝나는 것이 아니라, 도시전체의 흐름을 이해하고 체계적으로 연계되도록 구성되어야 한다. 본 연구는 시애틀의 블루링 프로젝트를 통하여 이러한 도심 가로구성체계에 대한 구체적 사례분석을 하여 그 물리적 구성방식과 계획적 특징을 분석하였다. 결과 시애틀의 가로체계는 개념적으로 스마트성장개념에 닿아 있으며, 물리적 특징으로 (1)보행자 중심의, (2)다양한 인간활동을 수용하는 오픈스페이스의 일부로서 역할하는, (3)외곽의 오픈스페이스와 연결되어 고립되지 않은, (4)보행자의 흐름이 끊어지지 않고 유기적으로 연계되는 위계적 구성을 가진, (5)지역적 디자인 요소를 최대한 활용한 것으로 분석되었다.

• Bulletin of the Korean Chemical Society
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• 제25권12호
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• pp.1898-1906
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• 2004

Nonclassical quinazolinone analogs I, II, and III, in which the glutamic acid moiety of the classical antifolates is substituted by phenylglycine, phenylalanine or aminobenzoic acid and their methyl esters, were synthesized and evaluated as lipophilic inhibitors of thymidylate synthase (TS). The target compounds were generally potent inhibitors of L. casei and human TS with $IC_{50}$ values within the narrow range of 0.2-10 ${\mu}$M and 0.003-0.03 ${\mu}$M, respectively. Further, most of the target compounds showed cytotoxicity against tumor cell lines of murine and human origin with $IC_{50}$ values of as low as 0.050 ${\mu}$M. Substitution of another hydroxyl or carboxylic acid/ester group at the phenyl ring further increased the potency of TS inhibition and cell growth inhibition. Most effective were compounds If and Ic in which extra carboxylic acid/ester was present at the phenyl ring with nanomolar $IC_{50}$ values of 0.0044 and 0.0093 ${\mu}$M against human TS and submicromolar cytotoxic growth inhibition against all four tumor cell lines.

• 통합자연과학논문집
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• 제10권3호
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• pp.141-147
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• 2017

Vascular endothelial growth factor (VEGF) is an important signaling protein involved in angiogenesis, which is the formation of new blood vessels from pre-existing vessels. Consequently, blocking of the vascular endothelial growth factor receptor (VEGFR-2) by small molecule inhibitors leads to the inhibition of cancer induced angiogenesis. In this study, we performed a two dimensional quantitative structure activity relationship (2D-QSAR) study of 38 N-Phenyl-N'-{4-(4-quinolyloxy) phenyl} urea derivatives as VEGFR-2 inhibitors based on hologram quantitative structure-activity (HQSAR). The model developed showed reasonable $q^2=0.521$ and $r^2=0.932$ values indicating good predictive ability and reliability. The atomic contribution map analysis of most active compound (compound 7) indicates that hydrogen and oxygen atoms in the side chain of ring A and oxygen atom in side chain of ring C contributes positively to the activity of the compounds. The HQSAR model developed and the atomic contribution map can serve as a guideline in designing new compounds for VEGFR-2 inhibition.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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• pp.78-78
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• 2012

In this presentation I will review the expanding thermal plasma chemical vapour deposition (ETP-CVD) technology, a deposition technology capable of reaching ultrahigh deposition rates. High rate deposition of a-Si:H, ${\mu}c$-Si:H, a-SiNx:H and silicon nanocrystals will be discussed and their various applications, mainly for photovoltaic applications demonstrated. An important aspect over the years has been the fundamental investigation of the growth mechanism of these films. The various in situ (plasma) and thin film diagnostics, such as Langmuir probes, retarding field analyzer, (appearance potential) mass spectrometry and cavity ring absorption spectroscopy, spectroscopic ellipsometry to name a few, which were successfully applied to measure radical and ion density, their temperature and kinetic energy and their reactivity with the growth surface. The insights gained in the growth mechanism provided routes to novel applications of the ETP-CVD technology, such as the ultrahigh high growth rate of silicon nanorystals and surface passivation of c-Si surfaces.

• 한국결정성장학회지
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• 제1권1호
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• pp.66-73
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• 1991

During the growth of macroscopically dislocation-free Czochralski silicon crystal, micro precipitates causing stacking faults in the silicon wafer during the oxidation are formed Thermal history the cryscausing acquire during the growth process is known to be a key factor determining the nucleation of this micro precipitates. In this article, various mechanisms suggested on the formation of microdefects in the silicon crystal are reviewed to secure the nucleation mechanism of the micro precipitates causing OSF whose pattern is normally ring or annular in CZ silicon crytal. B-defects which are known as vacancy clustering are considered to be the heterogeneous nucleation sites for the micro precipitates causing OSF in the CZ silicon crystals.