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1, 5 - bis (4 - methoxyphenyls) - 6, 7 - Dioxa-bicyclo [3.2.2] nonane's Anti-toxoplasmosis Effect that was Separated in Artemisin annula  

Jiang, Jing-Hua (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
Kim, Hwa-Kyoung (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
Kim, Hye-Sook (Faculty of Pharmaceuticalsciences, Okayama University)
Kamata, Masaki (Faculty of Education and Human Sciences Mathematical and Natural Sciences, Niigata University)
Wataya, Yusuke (Faculty of Pharmaceuticalsciences, Okayama University)
Park, Hyun (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 82-85 More about this Journal
Abstract
The Chinese medicinal plant Artemisia annua is the source of the antimalarial compound artemisinin. By the way, Artemisin annula was known have endoperoxide ring structure is included and has anti-malarial effect. Malaria and Toxoplasma gondii (T. gondii) is belong to Apicomplexa genera. So, confirmed whether we go compound 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane that have endoperoxide ring structure and there is anti-toxoplasmosis effect. The efficacy of 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2] nonane alone was examined in vitro and in a murine model of acute toxoplasmosis. In vitro studies were peformed with HeLa cell cultures, with quantification of Toxoplasma growth by a cell proliferation assay. Selectivity of 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane was 4.9 in vitro cell proliferation assay, this is higher than sulfadiazine (selectivity was 1.63). For in vivo studies, mice were acutely infected intraperitoneally with 10$^5$ tachyzoites of the virulent RH strain and then treated perorally for 4 days from 6 hours postinfection. Efficacy was assessed by sequential determination of parasite burdens in peritoneal cavity. in vitro, 1,5-bis(4-methoxyphenyl)-6,7-dioxa-bicyclo[3.2.2]nonane inhibited Toxoplasma growth at a concentration of 150mg/kg of body weight per day, the inhibition ratio was estimated to be 85.72%.
Keywords
Artemisia annua; Toxoplasma gondii; endoperoxide; in vitro; in vivo;
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