• 인간식물환경학회지
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• 제23권1호
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• pp.55-65
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• 2020

This study conducted a survey on 163 parents in Korea to investigate their interest in plants, experience in growing plants, and perception on horticultural activity programs (HAP) for their children. Most children (80.4%) of the respondents had experience participating in HAPs, and most of their experience occurred during school hours in preschool educational institutions more than once a month (60.3%). The more interest in plants and experience in growing plants the parents had, the more experience the preschoolers had in participating in HAPs, which implies that the experience of the parents with plants affected their children's participation in HAPs. Parents' most preferred location of HAPs was preschool educational institutions, and most preferred time was school hours with a frequency of more than once a week (57.1%), which is more frequent than the current status of HAPs in preschool educational institutions. Parents also responded that the most preferred instructors of HAP has are those with a professional background in horticulture. Among the activities in HAP for preschoolers, parents most preferred cooking (M = 4.05) activity, and they did not dislike any of the activities. They responded that the facilities for HAP needed to be improved to make HAP more active in preschool educational institutions. Overall, based on the current results, developing suitable HAPs and facilities for preschoolers, fostering professional HAP instructors, and educating parents about horticulture would enhance HAPs in preschool educational institutions, thus providing preschoolers with many benefits of HAPs.

• 분석과학
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• 제35권4호
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• pp.181-188
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• 2022

This paper describes a comparative analysis of yeast cell viability at exponential and stationary growth phases using multiple conventional techniques and statistical tools. Overall, cellular responses to various viability assays were asynchronous. Results of optical density measurement and direct cell counting were asynchronous both at exponential and stationary phases. Proliferative capacity measurement using SP-SDS indicated that cells at the end of the stationary phase were proliferative as much as exponentially growing cells. Metabolic activity assays using two different dyes concluded that the inside of cells at stationary phase is slightly less reducing compared to that of exponentially growing cells, implying that the metabolic activity imperceptibly declined as cells were aged. These results will be helpful to understand the details of yeast cell viability at exponential and stationary growth phases.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.65-71
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• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• 한국식품저장유통학회지
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• 제18권2호
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• pp.199-207
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• 2011

마늘의 산지별 품질특성 비교에 대한 기초자료를 확보하고자 국내의 한지형 및 난지형 마늘 주산지로부터 수집된 마늘 및 중국산 마늘의 물리적 특성, 향기성분과 항균활성을 비교 분석하였다. 마늘 구의 폭은 난지형 마늘이 50mm 이상으로 한지형 마늘에 비하여 넓었으며 평균 인편수도 난지형 마늘에서 6.38~9.31개로 더 많았다. 마늘의 표면색 중 L값은 80.73~87.40의 범위였으며, a값은 시료간의 유의적인 차이가 없었고, b값은 남해산과 무안산에서 가장 낮아 각각 $20.97{\pm}1.20$과 $20.91{\pm}1.62$였으며, 제주산이 $26.38{\pm}2.08$로 가장 높았다. 마늘의 향기성분을 분석한 결과 총 25~30개의 피크를 얻었으며, 이 중 GC-MS로 17종의 화합물을 동정할 수 있었고, 동정된 화합물 중 황화합물이 15종으로 대부분을 차지하였다. 마늘의 향기성분을 총 피크면적비로 계산한 결과 diallyl disulfide는 검출된 총 화합물 중 34.90~60.54%로써 타성분에 비해 월등히 높은 함량이었는데 중국산에서 가장 높게 검출되었으며 무안산에서 그 함량이 가장 낮았다. 다음으로 diallyl trisulfide가 17.55~38.72%였다. Strep. mutans, B. subtilis, E. coti, V. parahaemolyticus, Asp. flavus, C. albicans에 대해 항균활성을 실험한 결과 모든 마늘 시료에서 E. coli와 C. albicans에 대해 비교적 활성이 높았으며, 전체적으로 활성이 높았던 태안, 제주 및 남해마늘을 농도별로 가하여 항균활성을 실험한 결과 시료의 첨가량이 증가할수록 항균활성도 증가하였으며, 남해산 마늘에서 가장 항균활성이 높았다.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.324-328
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• 1998

We screened the methanol extracts from 133 plant species growing in Korea for antimicrobial activity against enterohemorrhagic Escherichia coli 0157 : H7. Those are selected from three plant grouping ; traditional medicinal herbs, edible plants, and flowers. They were tested by disk diffusion assay. From evaluation of the inhibition zone diameter of microbial growth, we found that the flower extract of Rhododendron Schilpenbachii Max had the most significant antimicrobial activity against this bacteria. Extracts from most of the vegetables and plants did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and seeds of Prunus Dallicina L. did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and the seeds of Prunus sallicina L.

• Archives of Pharmacal Research
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• 제27권9호
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• pp.885-892
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• 2004

Due to the rapidly growing number of resistant strains of bacteria, the search for antibacterial agents with new modes of action will always remain an important and challenging task. Thus, the reaction of 2-substituted or. unsubstituted-4-(4-acetylanilino)-5,6,7,8-terahydrobenzo［b］ thieno［2,3-d］pyrimidine derivatives 1-3 with the hydrazine derivatives, semi and / or thiosemi-carbazides, provided the corresponding hydrazones 4-6 and semi and/or thiosemicarbazones 7-9. Claisen-Schmidt condensation of compounds 1 or 2 with the appropriate aldehyde yielded the chalcones 10, 11 which, when treated with hydroxylamine hydrochloride gave rise to the isoxazoline-containing compounds 12, 13. Furthermore, reacting the respective chalcones 10, 11 with different hydrazines, urea and/or thiourea, furnished compounds 14, 15, 16, and 17 respectively. Representative compounds were tested for their antimicrobial activity against Candida Albicans and certain gram-positive and gram-negative bacteria. Their MICs were then determined. Compound 15e, showed a broad spectrum of activity while most of the other com-pounds showed varying antimicrobial activity.

• 한국정보시스템학회지:정보시스템연구
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• 제5권
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• pp.329-344
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• 1996

The role of Logistics Information Technology(LIT) activity within the firm has broadened considerably over the past decade and it been a topic of growing importance for both practitioners and academicians since the mid-1990's as logistics efficiencies have become a main concern in today's business management. This research empirically examines the difference between firm characteristics and LIT activity, thus offers an strategic guideline for practicers.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.369.2-369.2
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• 2002

Rumex crispus (Polygonaceae) is a well known perennial plant. which is called So-Ri-Jaeng-Yi. growing in the field and on the roadside. It has been used as a Korean Folk medicine in treating of acute and chronic cutaneous disease. cathartics, fever and jaundice. Also, the seed of this plant has been used as only a folk medicine for the treatment of digestion problems, liver diseases and many sorts of tumor. So we examined analgesic activity, anti-inflammatory activities and hepatoprotective activity using MeOH extraction and BuOH fraction in this plant. (omitted)

• 한국보육지원학회지
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• 제11권5호
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• pp.91-111
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• 2015

본 연구에서는 유아가 오감을 이용한 벼 관찰활동을 통해 유아의 과학과정기술이 어떻게 변화하는지 살펴보고자 실시되었다. 연구참여자는 경기도 오산시에 소재한 Y어린이집에 재원중인 만3세 유아 10명이었다. 유아의 과학과정기술의 변화를 살펴보기 위하여 Spradley(1980)의 참여관찰 분석과정을 최정열(2000)이 수정 보완한 분석과정을 사용하였다. 분석자료에는 유아 인터뷰 자료, 유아관찰일지, 교사 관찰기록 등이 사용되었다. 연구결과 오감을 이용한 벼 관찰활동은 유아의 과학과정기술에 긍정적인 효과가 있는 것으로 나타났다. 유아는 초기에 비해 후기로 갈수록 벼에 대해 예측하기, 관찰하기, 분류하기, 비교하기, 측정하기 능력이 향상되었다. 본 연구결과는 유아의 과학적 사고 신장을 위한 교수방법의 기초자료로 활용될 수 있을 것이다.

• Journal of Nutrition and Health
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• 제36권5호
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• pp.452-458
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• 2003

Soybean is a rich source of isoflavones such as genistein and daidzein. Soy isoflavones have both weak estrogenic and anti-estrogenic effects and are structurally similar to tamoxifen, an agent that has an effect similar to that of estrogen in terms of reducing postmenopausal bone loss. The purpose of this study was to determine the effects of differences in protein source (casein vs soy) and isoflavone levels (reduced vs higher levels) on selected bone markers and hormones in growing male rats. Thirty weanling Sprague-Dawley young rats were divided into 3 groups: The control group was fed a casein-based diet, the soy concentrate group was fed soy protein with totally reduced isoflavones content (isoflavones 0.07 mg/g protein), and the soy isolate group was fed soy protein with a higher than normal isoflavones content (isoflavones 3.4 mg/g protein). The degree of bone formation was estimated by measuring serum osteocalcin and alkaline phosphoatase (ALP). By determining collagen cross-linkage by immunoassay and correcting with creatinine values, the bone resorption rate was compared. Serum osteocalcin, growth hormone, estrogen and calcitonin were analyzed using radio immunoassay kits. The bone formation marker and ALP activity were differentiated by protein source, showing higher values than casein in feeding either soy isolate or soy concentrate. In this study using growing rats, the differences in isoflavone contents were not a significant factor in either bone formation or bone reaborption markers. Moreover, the soy isolate group had significantly higher levels of growth hormone than the casein group. The findings of this study suggest that growth hormone is partially responsible for its bone-formation effects in young growing rats. Soy protein and the isoflavones in soy protein are beneficial for bone-formation in growing male rats. Therefore, exposure to soy protein and isoflavones early in life may have long-term health benefits in preventing bone diseases such as osteoporosis. Further study to evaluate the mechanism of action of isoflavones on bones is warranted. (Korean J Nutrition 36(5): 452∼458, 2003)