• 생물환경조절학회지
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• 제16권4호
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• pp.372-378
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• 2007

본 연구는 칼륨의 시비농도를 인위적으로 조절하여 잎들깨의 생장과 결핍증상 발현에 미치는 영향을 구명하고, 생육을 우수하게 유지할 수 있는 식물체 및 토양의 한계농도를 밝히기 위하여 수행하였다. 칼륨 결핍 증상은 노엽에서 시작되었으며, 노엽의 가장자리가 황변하고, 황변된 부위가 점차 갈색으로 변화되었으며, 갈변 후 괴사하였다. 칼륨 시비농도를 8mM까지 높일 경우 엽장과 줄기직경이 길거나 굵어졌으며, 생체중 및 건물중이 무거워지고, 엽록소 함량이 감소하였다. 생육이 우수하였던 K 8mM 시비구의 식물체당 건물중과 K 함량이 5.01g과 2.76%였으며, 생장억제를 방지하기 위해서는 1.7% 이상의 K 함량을 가져야 한다고 판단되었다. 칼륨을 8mM로 시비한 처리의 엽병추출액내 K 농도가 $12,289mg{\cdot}kg^{-1}$였고, 1:2로 추출한 토양농도가 $11.65mg{\cdot}L^{-1}$였으며, 엽병추출액은 $8,700mg{\cdot}kg^{-1}$이상, 토양용액은 $4.5mg{\cdot}L^{-1}$을 유지하도록 시비하여야 수량 감소를 방지할 수 있다고 판단되었다.

• Applied Biological Chemistry
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• 제18권2호
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• pp.56-60
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• 1975

완두유묘의 자엽 및 뿌리조직중 탄소-14 방사능을 Liquid scintillation counter로 신속히 측정하는 새로운 방법에 관하여 실험하였다. 식물조직을 액체공기에 처리하여 분쇄한 다음 Gel phosphor에 헌탁시켜, Liquid scintillation counter로 측정하였다. 이시료에 다시 내부표준물질을 가한 다음 계측효율을 구하여 Apparent absolute activity를 얻었다. 이렇게 얻은 헌탁시료의 절대방사능은 자기 흡수현상 때문에, 따로 Flask combustion 방법으로 구한 절대방사능 보다는 낮았으나, 항상 비례하였다. 따라서 동일한 실험조건하에서 상술한 비율을 얻으면, 완두조직중 탄소-14의 점대방사능은, 비교적 간편한 헌탁측정(suspension counting) 만으로 가능하였다. 본방법은 완두 외에 다른 식물조직중 탄소-14 분석에도 쓰일 수 있다.

• Journal of Plant Biology
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• 제30권3호
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• pp.189-199
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• 1987

Morpho-histogenesis of fruit sculpture and dehiscence in Thespesia populnea is described. The fruit wall is differentiated into epicarp, mesocarp and endocarp. The epicarp is stony, rind-like, 30 to 35 layers thick and derived from outer epidermis, sub-epidermis and ground parenchyma of the ovary wall. The spherical and/of tangentially elongated, thick walled cells of epicarp are interspersed with radial bands of sclereids. The mesocarp is a product of the inner zone of ground parenchyma. At maturity 20 to 25 layers of thin walled parenchyma of mesocarp appear sinuous of disorgnized. The innermost 1 to 3 layers of ground parenchyma and sub-epidermis and inner epidermis form 35 to 40 layers thick endocarp. Due to the differentiation of fibrous tissue in the projection of median plane of carpel wall and a complete ring of fibrous zone in the endocarp, the dry capsule of Thespesia populnea dehises partially in loculicidal fashion.

• 대한치과교정학회지
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• 제14권1호
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• pp.103-113
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• 1984

This work was undertaken to evaluate the integumental response in lower face to hard tissue changes, and to grope the prediction equation for expected integumental profile changes. Cephalometric headplates of 25 persons consisted of 8 Angle's class 1 maxillary protrusive and 17 Angle's class II division 1 patients whose mean age was 15.2 years were traced, diagramatized, and statistically analyzed. The results were as follows; 1. Upper incisor and lips were retracted and convexity of integumental profile decreased concurrently with decrease of hard tissue procumbency, however soft tissue point A', B', and Pog' did not undergo significant changes after orthodontic treatment. 2. Remarkable increment of upper lip thickness and upper lip height was shown and this was related to upper incisor retraction. The ratio between the amount of upper incisor retraction and the increment o f upper lip thickness was approximately 1.16:1. 3. Moderate correlation of upper lip retraction to upper incisor retraction, and of lower lip retraction to lower incisor movement were arranged, and yet comparatively wide variability from subject to subject was shown. 4. It was possible to predict statistically for horizontal alteration of lip position and change of upper lip angulation ground in orthodontic treatment.

• Smart Structures and Systems
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• 제7권3호
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• pp.213-222
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• 2011

The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.

• Journal of Astronomy and Space Sciences
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• 제37권4호
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• pp.249-255
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• 2020

We develop the tissue-equivalent proportional counter (TEPC) type's space radiation dosimeter to measure in-situ aviation radiation. That was originally developed as a payload of small satellite in the low-earth orbit. This dosimeter is based on a TEPC. It is made of an A-150 tissue-equivalent plastic shell of an internal diameter of 6 cm and a thickness of 0.3 cm. TEPC is filled with pure propane at 13.9 torrs to simulate a cell diameter of 2 ㎛. And the associated portable and low power electronics are also implemented. The verification experiments have been performed by the calibration experiments at ground level and compared with Liulin observation at aircraft altitude during the flight between Incheon airport (ICN) and John F. Kennedy airport (JFK). We found that the TEPC dosimeter can be used as a monitor for space radiation dosimeter at aviation altitude based on the verification with Liulin observation.

• Journal of Chest Surgery
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• 제53권5호
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• pp.250-257
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• 2020

Background: Lung adenocarcinoma (LUAD) with ground-glass opacity (GGO) can become aggravated, but the reasons for this aggravation are not fully understood. The goal of this study was to analyze the genetic features and causes of progression of GGO LUAD. Methods: LUAD tumor samples and normal tissues were analyzed using an Illumina HiSeq 4000 system. After the tumor mutational burden (TMB) was calculated, the identified mutations were classified as those found only in GGO LUAD, those present only in nonGGO LUAD, and those common to both tissue types. Ten high-frequency genes were selected from each domain, after which protein interaction network analysis was conducted. Results: Overall, 227 mutations in GGO LUAD, 212 in non-GGO LUAD, and 48 that were common to both tumor types were found. The TMB was 8.8 in GGO and 7.8 in non-GGO samples. In GGO LUAD, mutations of FCGBP and SFTPA1 were identified. FOXQ1, IRF5, and MAGEC1 mutations were common to both types, and CDC27 and NOTCH4 mutations were identified in the non-GGO LUAD. Protein interaction network analysis indicated that IRF5 (common to both tissue types) and CDC27 (found in the non-GGO LUAD) had significant biological functions related to the cell cycle and proliferation. Conclusion: In conclusion, GGO LUAD exhibited a higher TMB than non-GGO LUAD. No clinically meaningful mutations were found to be specific to GGO LUAD, but mutations involved in the epithelial-mesenchymal transition or cell cycle were found in both tumor types and in non-GGO tissue alone. These findings could explain the non-invasiveness of GGO-type LUAD.

• 재활복지공학회논문지
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• 제10권4호
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• pp.259-265
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• 2016

본 논문에서는 폐질환 조기 검출을 위하여 Broncho vascular, Emphysema, Ground Glass Reticular, Ground Glass, Honeycomb, Normal의 6가지 폐조직에 대한 새로운 분류기법을 제안하였다. 단순 베이즈 분류기와 아다부스트 학습 기법을 도입하여 459개의 결합 히스토그램 특징인자로부터 유효한 특징인자를 선별함으로써 폐조직을 분류하였다. 다중 해상도 해석, 체적 LBP 및 CT 휘도를 기반으로 하는 결합 히스토그램 특징인자는 정확도, 민감도, 특이도 결과에서 기존의 3D AMFM보다 우수한 결과를 보였다. 제안한 특징인자와 3D AMFM 특징인자의 정확도는 각각 90.1%과 85.3%로서 제안한 특징인자의 우수한 분류 성능을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.385-392
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• 2007

A glucose clamp technique was used to compare dietary starch (ST), starch plus sucrose (ST+SU) and sucrose (SU) with regard to the effect on tissue responsiveness and sensitivity to insulin in intact adult male goats. The goats were fed diets containing 1.2 times of ME and CP for maintenance requirements twice daily for 21 d. Of the energy intake, 30% was offered with ST, ST+SU or SU for the respective diets, and 70% as alfalfa hay, ground corn and ground soybean meal at the respective weight ratio of 1, 1, and 0.3 for all diets. Tissue responsiveness and sensitivity to insulin were evaluated using a hyperinsulinemic euglycemic clamp technique with four levels of insulin infusion beyond 13 h after feeding. The concentrations of plasma metabolites and insulin were also determined at 3, 6 and 13 h after feeding to evaluate the effects of different carbohydrates on metabolic states in the body. Plasma glucose concentration was higher (p = 0.01) for SU diet than for ST and ST+SU diets. Increasing SU intake decreased (p<0.01) plasma acetate concentration across the time. At 3 h but not 6 and 13 h after feeding, high lactate (p = 0.01), and non-significant high propionate (p = 0.14) and low urea nitrogen (p = 0.19) concentrations were observed in plasma on SU compared with ST and ST+SU diets. Plasma insulin concentration was not different (p = 0.44) between ST and SU fed animals. In the glucose clamp experiment, considering the effects on the maximal glucose infusion rate (tissue responsiveness to insulin, p = 0.54) and the plasma insulin concentration at half-maximal glucose infusion rate (insulin sensitivity, p = 0.54), SU was not different from ST. It is concluded that SU may not be greatly different from ST with regard to the effect on tissue responsiveness and sensitivity to insulin in adult goats when fed twice daily as part of a high-concentrate diet. The possible greater effects of SU on plasma metabolites concentrations at 3 h than at 6 and 13 h after feeding suggest that a lack of persistency of SU effects during the postfeeding period may be associated with the poor response to SU in insulin action.

• ALGAE
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• 제19권2호
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• pp.123-127
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• 2004

A more rapid and efficient method to extract RNA from Ecklonia cava Kjellman (Laminariales, Phaeophyceae) was introduced in this study. Each step of the procedure was evaluated and the optimal concentration of each chemical in the lysis solution was determined. Tissue pulverization with PVPP and β-mercaptoethanol in the lysis solution were not essential for RNA extraction of this species. The highest yield and purity of E. cava RNA were obtained by the lysis solution containing 1% CTAB, 1 M NaCl, 0.7% PVP, 10mM EDTA and 100mM Tris-Cl (pH 9.0). Approximately 8μg of RNA was obtained from 200 mg of ground tissue. The ratios of the absorbance at 260 nm and 280 nm were from 1.6 to 1.8 and those of at 230 nm and 260 nm were from 1.8 to 2.0. The extracted RNAs obtained in this study turned out to have a sufficient quality for cDNA synthesis.