• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.170-170
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• 2001

This study was designed to evaluate a repeated intravenous dose toxicity of a new anticancer agent, SB extracted from Pulsatilla korean Nakai in Beagle dogs. Animals were intravenously injected with dosages of 0, 0.062, 0.25, and 1 mg/kg of SB everyday for 13 weeks, respectively. There were no dose-related changes in clinical signs, body weight changes, food and water consumptions, opthalmoscopy, and urine analysis. There were somewhat significant differences compared with control group in organ weight, biochemical examination, and hematology findings of animals treated with SB. However, these changes were not dose-related changes. Gross and histopathological findings revealed no evidence of specific toxicity related to SB. These indicate that intraveous maximum tolerated dose value of SB may be over 1mg/kg in rats.

• Korean Journal of Pharmacognosy
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• v.42 no.3
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• pp.265-270
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• 2011

Acute toxicity of a combined preparation of the standardized extracts Scutellaria baicalensis GEORGI and Salvia miltiorrhiza BUNGE in a ratio of 3:1 was examined in male and female ICR mice. Mice were treated with the test substance intragastrically at a dose of 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. No death or abnormal clinical sign was shown during the observation period. Also there were no difference in net body weight gain, organ weight, and gross pathological findings at the terminal sacrifice. The results suggested that acute oral toxicity of a combined preparation of the standardized extracts is very low at the conditions employed in this study.

• Toxicological Research
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• v.27 no.4
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• pp.261-267
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• 2011

Artemisia iwayomogi, a member of the Compositae, is a perennial herb easily found in Korea and used as a traditional medicine to treat liver disease. AIP1, a water-soluble carbohydrate fraction from Artemisia iwayomogi, showed anti-tumor and immuno-modulating activities in animal studies. A subacute toxicological evaluation of AIP1 was performed for 4 weeks in ICR mice. After administration of AIP1 (0, 20, 100, 500 mg/kg/day), the clinical signs, mortalities, body weight changes, hematology, blood clinical biochemistry, urinalysis, organ histopathology, organ weights and gross finding were examined. The results showed that there were no significant differences in body weight changes, food intakes, water consumptions, or organ weights among different dose groups. Also we observed no death and abnormal clinical signs during the experimental period. Between the groups orally treated with AIP1 and the control group, there was no statistical significance in hematological test or serum biochemical values. Histopathological examination showed no abnormal changes in AIP1 groups. These results suggest that no observed adverse effect level (NOAEL) of the oral administration of AIP1 for 4 weeks was considered to be more than 500 mg/kg/day in mice under the condition investigated in current study.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.358-361
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• 2004

This paper describes the development process of high stiffness body for ride and handling performance. High stiffness and light weight vehicle is a major target in the refinement of passenger cars to meet customers' contradictable requirements between ride and handling performance and fuel economy. This paper describes the analysis approach process for high stiffness body through the data level of body stiffness. According to the frequency band, we can suggest the design guideline about Is cornering static stiffness, torsional and lateral stiffness, body attachment stiffness. The ride and handling characteristic of a vehicle is significantly affected by vibration transferred to the body through the chassis mounting points from front and rear suspension. It is known that body attachment stiffness is an important factor of ride and handling performance improvement. And high stiffness helps to improve the flexibility of bushing rate tuning between Handling and road noise. It makes it possible to design the good handling performance vehicle at initial design stage and save vehicles to be used in tests by using mother car at initial design stage. These improvements can lead to shortening the time needed to develop better vehicles.

• Journal of the Korea institute for structural maintenance and inspection
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• v.4 no.2
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• pp.103-111
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• 2000

The objectives of the study are to know the strain distribution and modal dynamic behaviour of steel bridge girders by actual traffic load. The live load effect depends on many parameters including the span length, gross vehicle weight, axle weight, axle configuration so on. For the estimation of static and dynamic characteristic, strain data caused by moving loads and traffic characteristics of passing vehicle under actual traffic load have measured using Bridge Weigh in Motion. To confirm the reliability of BWIM system, strain data measured using the $120{\Omega}$ strain gauge under the same condition. It is considered that the data acquired from BWIM system have reliability through the analysis and comparison between stress measured by strain data from BWIM and computed by FEM. Additionally according to the measured strain data of up-line and down-line on the highway, the up-line bridge grows more faster than the down-line bridge and girder 4 and 5 carry more load when vehicles pass the inner line and girder 2 and 3 does when vehicles pass the outer line as this case(the bridge composed with 5 girders).

• Journal of Pharmacopuncture
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• v.26 no.1
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• pp.86-93
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• 2023

Objectives: This study aimed to evaluate the potential toxicity of a recently developed and clinically used No-Pain pharmacopuncture (NPP) solution. We also assessed the lethal dose of the NPP agent following a single intramuscular injection in Sprague-Dawley (SD) rats. Methods: Animals were divided into two groups: the NPP test material group and the normal saline control group. A single intramuscular injection of the NPP agent (1.0 mL/animal) was administered to rats of the NPP test material group. The control group rats received the same volume of normal saline. Both female and male rats were included in each group. All rats were monitored for clinical signs and body weight changes for 14 days after administration of the test substance or saline. At the end of the observation period, a gross necropsy was conducted and localized tolerance at the injection site was analyzed. Results: No mortality was observed in the NPP test material and control groups. Moreover, no test substance-related effects were observed on clinical signs, body weight, necropsy findings, and localized tolerance at the injection site. Conclusion: The approximate lethal dose of the NPP agent is greater than 1.0 mL/animal under the conditions used in this study. Additional toxicity evaluations and clinical studies are needed to confirm the safety of NPP use in clinical practice.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.7
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• pp.1008-1013
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• 2001

This experiment was conducted to determine the effects of enzyme supplementation on the performance of 80 growing-finishing pigs (26.2 kg) fed diets containing either soybean or canola meal. Barley-based diets formulated using either soybean meal or canola meal were fed with or without enzyme (Allzyme Vegpro, Alltech Biotechnology Centre). Eight castrates and twelve gilts were fed each diet. Digestibility of dry matter, crude protein and gross energy was 8.0 (p=0.0001), 7.9 (p=0.0005) and 7.9 (p=0.0003) percent lower for pigs fed diets containing canola meal compared with soybean meal. Enzyme supplementation had no effect on nutrient digestibility (p>0.05). There was a significant interaction between protein source and enzyme for all three nutrients. Over the entire experimental period (26.2 to 77.9 kg), pigs fed canola meal consumed 9.4% less feed (p=0.001), gained weight 20.4% slower (p=0.001) and had a 12.9% poorer feed conversion (p=0.001) than pigs fed soybean meal. Weight gain, feed intake and feed conversion were unaffected by enzyme addition (p>0.05). Castrates gained weight 11.4% faster (p=0.001), consumed 9.3% more feed (p=0.001) and had a 2.6% better feed conversion (p=0.026) than gilts. There was a significant interaction between protein source and sex of pig for feed conversion. Pigs fed diets based on canola meal had a significantly lower carcass value index (p=0.01), lower lean yield (p=0.007) and lower lean depth over the loin (p=0.001) than pigs fed diets based on soybean meal. Enzyme addition significantly increased lean depth over the loin (p=0.01). There was a significant interaction between protein source and enzyme for carcass value index (p=0.04), estimated lean yield (p=0.05) and fat depth over the loin (p=0.05). These results confirm previous studies which have demonstrated poorer pig performance when canola meal completely replaces soybean meal in diets fed to growing-finishing pigs. In addition, the results provide little justification for the inclusion of the Vegpro enzyme in diets fed to pigs of this weight range.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.21 no.1
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• pp.33-39
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• 2011

The present study was carried out to investigate the potential acute toxicity of methylcyclohexane (MCH) by a single oral dose in female rats. The test chemical was administered once by gavage to female rats at dose levels 0, 1,250, 2,500, and 5,000 mg/kg. Mortalities, clinical findings, and body weight changes were monitored for the 14-day period following the administration. At the end of 14-day observation period, all animals were sacrificed and complete gross postmortem and histopathological examinations were performed. Treatment-related clinical signs, as evidenced by depression, soft feces, decreased locomotion activity, solid perineal region, crouching position, and anorexia were observed in all treatment groups in a dose-dependent manner. At the dose level of 5,000 mg/kg, decreased or suppressed body weight gain was found during the study period. At the scheduled necropsy, one case of congestion of the intestine and an increase in the weights of liver and kidney were observed in the 5,000 mg/kg group. Histopathological examinations exhibited an increased incidence of glomerular atrophy, congestion/hemorrhage, and focal degeneration/necrosis in the liver and an increased incidence of congestion, and inflammatory cell infiltration in the kidney. On the basis of the results, it was concluded that a single oral administration of MCH resulted in some adverse effects on clinical sign, body weight gain, and organ weight and histopathology in the liver and kidney in female rats. In the experimental conditions, the minimal lethal dose ($LD_{10}$) of MCH was greater than 5,000 mg/kg.

• The Journal of Korean Medicine
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• v.26 no.2 s.62
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• pp.32-51
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• 2005

Objectives: To study the effect of Artemisiae Capillaris Herba extracts, that have been used as oriental medicine to treat liver disease, on the perinatal and lactational n;)productive toxicity of SD rats when administered by oral lavage. Methods: Female SD rats were dosed from 6 days of gestation to 3 weeks postpartum. This was conducted in accordance with the recommendations of the KFDA Guidelines for Detection of Toxicity to Reproduction for Medicinal Products. Results: No Artemisiae Capillaris Herba extracts treatment-related changes in clinical signs, mortalities, implantation number, dead fetus number, loss rate of fetus, number of live young, survival rate of fetus, sex ratio of live young, external anomalies, pregnancy periods, viability index, lactational index, survival rate of litters at 4 days after birth or delivery index were demonstrated in any dosed levels in this study. However, the body weight and gains, food consumption and absolute organ weights of brain, adrenal glands, liver, spleen, kidney, ovaries and heart were significantly increased in 2000 or 1000mg/kg-dosing groups and the relative organ Weights of adrenal glands were significantly increased in 2,000mg/kg-dosing groups. Therefore, it was concluded that this increase was natural according to growth. Also, no changes of gross findings, clinical signs, mortalities, body weight and gains, physical development results, necropsy findings, organ weight, faculty test, open filed test and water-filled simple T-maze test, copulation, fertility, pregnancy indices, body weight and gains during gestation periods, necropsy findings, corpora lutea number, implantation number, implantation rate, dead fetus number, post-implantation loss rate, live young, post-implantation survival rate, sex ratio of live young, external anomalies and individual body weights of live young were demonstrated in any dosed levels in this study. Conclusions: It is considered that the NOAEL (No-Observed-Adverse-Effect Level) for perinatal and lactational reproductive toxicity of Artemisiae Capillaris Herba extracts was up to 2000mg/kg/day because no changes of other perinatal and lactational reproductive indices were demonstrated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.389-394
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• 2017

In a conventional ship structure, stiffeners with an asymmetric section, such as inverted angles, are used widely despite the disadvantage of strength compared to the stiffeners with a symmetric section, such as a built-up T. On the other hand, T-type built-up members are attracting more attention than L-type inverted angles due to the increased size of ships. The purpose of this study was to develop an optimal design program for a built-up T, and apply an evolution strategy as an optimization technique. In the optimization process, the gross thickness concept was adopted for the design variables and objective function, and the constraints are set up based on HCSR (Harmonized Common Structural Rules). Using the developed program in this study, the optimal stiffener design was carried out for 300K VLCC and 158K COT of which the orders were obtained lately. The optimal results revealed the weight reduction effect of 144 tons and 60 tons, respectively.