한국산업보건학회지 (Journal of Korean Society of Occupational and Environmental Hygiene)

  • 제21권1호
  • /
  • Pages.33-39
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  • 2011
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  • 2384-132X(pISSN)
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  • 2289-0564(eISSN)
한국산업보건학회 (Korean Industrial Hygiene Association)
권호 표지

암컷 랫드에서 Methylcyclohexane의 단회 경구투여 독성시험

Single Oral Dose Toxicity Study of the Methylcyclohexane in Female Rats

  • 김성환 (전남대학교 수의과 대학) ;
  • 임정현 (전남대학교 수의과 대학) ;
  • 신인식 (한국한의학연구원 한약EBM연구센터) ;
  • 문창종 (전남대학교 수의과 대학) ;
  • 김성호 (전남대학교 수의과 대학) ;
  • 신동호 (전남대학교 수의과 대학) ;
  • 김종춘 (전남대학교 수의과 대학)
  • Kim, Sung-Hwan (College of Veterinary Medicine, Chonnam National University) ;
  • Lim, Jeong-Hyeon (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, In-Sik (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Changjong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Dong-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University)
  • 투고 : 2011.01.03
  • 심사 : 2011.03.05
  • 발행 : 2011.03.31
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초록

The present study was carried out to investigate the potential acute toxicity of methylcyclohexane (MCH) by a single oral dose in female rats. The test chemical was administered once by gavage to female rats at dose levels 0, 1,250, 2,500, and 5,000 mg/kg. Mortalities, clinical findings, and body weight changes were monitored for the 14-day period following the administration. At the end of 14-day observation period, all animals were sacrificed and complete gross postmortem and histopathological examinations were performed. Treatment-related clinical signs, as evidenced by depression, soft feces, decreased locomotion activity, solid perineal region, crouching position, and anorexia were observed in all treatment groups in a dose-dependent manner. At the dose level of 5,000 mg/kg, decreased or suppressed body weight gain was found during the study period. At the scheduled necropsy, one case of congestion of the intestine and an increase in the weights of liver and kidney were observed in the 5,000 mg/kg group. Histopathological examinations exhibited an increased incidence of glomerular atrophy, congestion/hemorrhage, and focal degeneration/necrosis in the liver and an increased incidence of congestion, and inflammatory cell infiltration in the kidney. On the basis of the results, it was concluded that a single oral administration of MCH resulted in some adverse effects on clinical sign, body weight gain, and organ weight and histopathology in the liver and kidney in female rats. In the experimental conditions, the minimal lethal dose ($LD_{10}$) of MCH was greater than 5,000 mg/kg.

키워드

과제정보

연구 과제 주관 기관 : 교육과학기술부

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