• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.856-863
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• 2013

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

• Journal of Plant Biotechnology
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• v.29 no.2
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• pp.117-121
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• 2002

We have developed an in vitro micropropagation system via shoot formation from axillary buds using nodal segments of Corylopsis coreana. Explants from both juvenile tree (one-year-old greenhouse stock seedlings) and mature tree (ten-years-old tree in nursery) were compared with regard to propagation efficiency. Combined treatment of both BA and zeatin were effective on shoot proliferation since the best result was obtained on MS medium supplemented with 0.5∼3.0 mg/L zeatin and 0.2 mg/L BA. Generally, juvenile explants were better in both shoot proliferation and growth than mature explants. However, as the duration of in vitro culture was proceed to 6 months, explants from mature tree also produced three shoots per explant. Distinctive differences in rooting and adaptability to soil of shoots obtained from mother trees. Whereas shoots originated from juvenile explants rooted as high as 97%, those from adult explants showed 62% rooting. Similar result was also observed in soil acclimatization. The plantlets derived from juvenile plants survived 67%, while only 48% of those from adult trees survived. The results showed a possibility of the micropropagation of Corylopsis coreana through shoot formation from axillary buds. In addition, the advance of the research still remain to enhance the frequency of acclimatization of plantlets from mature trees for practical application.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.19-28
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• 2014

Research was initiated in greenhouse to investigate effects of polymer, calcium, perlite and chitosan on the growth of Kentucky bluegrass (KB). A total of 24 treatment combinations were used in the study. Treatments were made of water-swelling polymer (WSP), calcium, perlite, and chitosan in soil organic amendment (SOA). Significant differences were observed in germination rate, turfgrass coverage, turfgrass density and top growth among treatments. Germination rate, density and plant height varied with time after seeding. A proper mix of WSP is considered to be lower than 3% for turfgrass coverage and density. Regarding survival capability and top growth, however, it was good under 6%. Overall KB growth was more influenced by calcium and perlite than chitosan. Calcium and perlite were the most effective elements for early survival capacity and turfgrass density, respectively. But no effect was found by chitosan. Top growth increased with three elements, being perlite > calcium > chitosan. The chitosan was effective in early germination, but there was no effect on top growth until 3 weeks, when compared with others. A further study is needed for investigating the effect of these materials on the growth characteristics in mixtures of sand and SOA before a field application.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.2
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• pp.141-146
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• 1982

Laboratory and greenhouse experments were conducted to find out the optimum dilution time of methane fermented effluent of hog waste which was high pH (7.85), $NH_4$-N (683ppm), electric conductivity (8.3mmhos/cm) and BOD (about 1,000ppm) and the interval of effluent change which will provide maximun yield of green algae (Scenedesmus accuminatus), duck weed (Lemner minor L.) and Azolla anabaena (Azolla pinnata). The results of this experiment are summerized as follows. 1. The maximum yield were obtained at 5 times diluted effluent ($NH_4$-N : 137ppm) and exchange of 4 day intertval, from 25 times to 50 times dilution ($NH_4$-N : 7-14ppm) and 4 day interval, and 50 times dilution ($NH_4$-N : 14 ppm) and exchange the effluent at 4-8 day interval for green algae, duck weed and azolla growth, respectively. 2. Purification of the effluent was enhenced by growth of the green algae, duck weed and azolla due to decrease of pH, $MH_4$-N and electric conductivity of effluent. The degree of purification was higher in case of azolla growth than duck week growth.

• Korean Journal of Weed Science
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• v.14 no.1
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• pp.34-41
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• 1994

To better understand the allelopathic effect of sorghum(Sorghum vulgare L.), the inhibitory activities of water extracts of the stem, leaf and root, and of residues of the stem to major crops and weeds associated with them were evaluated. The allelopathic activity of sorghum plants was species specific, and depended on source and concentration. Germination, and shoot and root length of all test species were inhibited by the different concentrations of the stem extract. Among the crop species, radish showed the most inhibition, followed by wheat and rice. Maize was the least sensitive species. Of the weed species, Ipomoea triloba was most inhibited, followed by Echinochloa colona and Rottboellia cochinchinensis. The water extracts of leaves, stems, and roots significantly inhibited germination and seedling growth in E. colona and radish. The stem extract gave the greatest inhibitory effect on E. colona while all three extracts produced similar response in radish. In the greenhouse trial, sorghum stem residue placed on the soil surface as mulch significantly inhibited seedling growth in E. colona and radish, but not that in rice.

• The Korean Journal of Mycology
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• v.42 no.3
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• pp.225-230
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• 2014

Sclerotinia sclerotiorum, a plant pathogenic fungus, can cause serious yield and quality losses in the winter lettuce field. For biological control of S. sclerotiorum, soil-born microorganisms that inhibit the mycelia growth of S. sclerotiorum and Fusarium oxysporum were isolated from diseased soil. Among the isolates, bacterial isolate, GG95, which was identified as Bacillus subtilis according to the morphological, physiological characteristics and by 16S rRNA similarity, showed the highest level of inhibitory activity. The growth conditions for B. subtilis GG95 were optimized in TSB media (pH 7) by culturing at $28^{\circ}C$ for 24 hrs. Maltose or fructose and peptone were selected as the best carbon and nitrogen sources, respectively. Greenhouse experiment was performed to test effectiveness of B. subtilis GG95 in the control sclerotinia rot. Drench application ($1{\times}10^8cfu/mL$, 3 times) of the bacterial culture broth to lettuce showed an effectiveness value of 88%, suggesting that B. subtilis GG95 would be a promising biocontrol agent for control of sclerotinia rot.

• Journal of Biosystems Engineering
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• v.25 no.3
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• pp.213-220
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• 2000

Grafting of fruit-bearing vegetables has been widely used to increase the resistance to soil-borne diseases, to increase the tolerance to low temperature or to soil salinity, to increase the plant vigor, and to extend the duration of economic harvest time. After grafting, it is important to control the environment around grafted seedlings for the robust joining of a scion and rootstock. Usually the shading materials and plastic films are used to keep the high relative humidity and low light intensity in greenhouse or tunnel. It is quite difficult to optimally control the environment for healing and acclimation of grafted seedlings under natural light. So the farmers or growers rely on their experience for the production of grafted seedling with high quality. If artificial light is used as a lighting source for graft-taking of grafted seedlings, the light intensity and photoperiod can be easily controlled. The purpose of this study was to develop a prototype system for the graft-taking enhancement of grafted seedlings using artificial lighting and to investigate the effect of air current speed on the distribution of air temperature and relative humidity in a graft-taking enhancement system. A prototype graft-taking system was consisted by polyurethane panels, air-conditioning unit, system controller and lighting unit. Three band fluorescent lamps (FL20SEX-D/18, Kumho Electric, Inc.) were used as a lighting source. Anemometer (Climomaster 6521, KANOMAX), T-type thermocouples and humidity sensors (CHS-UPS, TDK) were used to measure the air current speed, air temperature and relative humidity in a graft-taking system. In this system, air flow acted as a driving force for the diffusion of heat and water vapor. Air current speed, air temperature and relative humidity controlled by a programmable logic controller (UP750, Yokogawa Electric Co) and an inverter (MOSCON-G3, SAMSUNG) had an even distribution. Distribution of air temperature and relative humidity in a graft-taking enhancement system was fairly affected by air current speed. Air current speed higher than 0.1m/s was required to obtain the even distribution of environmental factors in this system. At low air current speed of 0.1m/s, the evapotranspiration rate of grafted seedlings would be suppressed and thus graft-taking would be enhanced. This system could be used to investigate the effects of air temperature, relative humidity, air current speed and light intensity on the evaportranspiration rate of grafted seedlings.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.3
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• pp.141-148
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• 2021

Agrivoltaic systems, also called solar sharing, stated from an idea that utilizes sunlight above the light saturation point of crops for power generation using solar panels. It is expected that agrivoltaic systems can realize climate smart agriculture by reducing evapotranspiration and methane emission due to the reduction of incident solar radiation and the consequent surface cooling effect and bring additional income to farms through solar power generation. In this study, to evaluate that agrivoltaic systems are suitable for realization of climate smart agriculture, we conducted agro-environmental observations (i.e., downward/upward shortwave/longwave radiations, air temperature, relative humidity, water temperature, soil temperature, and wind speed) in a rice paddy under an agrivoltaic system and compared with the environment outside the system using automated meteorological observing systems (AMOS). During the observation period, the spatially averaged incoming solar radiation under the agrivoltaic system was about 70% of that in the open paddy field, and clear differences in the soil and water temperatures between the paddy field under the agrivoltaic system and the open paddy field were confirmed, although the air temperatures were similar. It is required in the near future to confirm whether such environmental differences lead to a reduction in water consumption and greenhouse gas emissions by flux measurements.

• Korean Journal of Organic Agriculture
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• v.29 no.1
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• pp.111-123
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• 2021

This study was conducted to investigate the growth characteristics of strawberries and N₂O emission by treating the compost for each type of livestock manure, which was an organic farming material, as a basal fertilization in plastic film house. Livestock manure compost, which made from cattle manure, swine manure, and poultry manure as raw materials, were applied to this experiment, treated by mixing or single on the basis of nitrogen content with the standard amount of fertilizer for strawberries. Total emission of N₂O were 10.7% higher than those in poultry manure compost treatment compared to the inorganic fertilizer treatment, but 16.5~41.9% lower than those in other livestock manure compost treatment. The period of N₂O emission mainly was up to the 17th day after fertilizer application, accounting for 70~87% of the total amount of discharge, and 13~30% of the total amount was emitted for 158 days later. N₂O emission was decreased significantly NH₄⁺-N content in the soil, and increased NO₃^--N. As compared with control, the number of leaves, leaf width and crown diameter of livestock manure compost treatments were not significantly different, leaf length of cattle+poultry, cattle+ swine, swine+poultry treatment higher, and SPAD (soil plant analysis development) values of cattle+poultry treatment highest. There was no significant difference in weight and sugar content of strawberry fruits among treatments.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.502-509
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• 2013

To estimate greenhouse gas (GHG) emission, we established inventory of conventional rice cultivation from farmers in Gunsan and Iksan, Jeonbuk province in 2011~2012. This study was to calculate carbon footprint and to analyse the major factor of GHGs. We carried out a sensitivity analysis using the analyzed main factors of GHGs and estimated the mitigation potential of GHGs. Also we tried to suggest agricultural methods to reduce GHGs that farmers of this case study can apply. Carbon footprint of rice production unit of 1 kg was 2.21 kg $CO_2.-eq.kg^{-1}$. Although amount of $CO_2$ emissions is largest among GHGs, methane had the highest contribution of carbon footprint on rice production system after methane was converted to carbon dioxide equivalent ($CO_2$-eq.) multiplied by the global warming potential (GWP). Source of $CO_2$ in the cultivation of rice farming is incomplete combustion of fossil fuels used by agricultural machinery. Most of the $CH_4$ emitted during rice cultivation and major factor of $CH_4$ emission is flooded paddy field in anaerobic condition. Most of the $N_2O$ emitted from rice cultivation process and major sources of $N_2O$ emission is application of fertilizer such as compound fertilizer, urea, orgainc fertilizer, etc. As a result of sensitivity analysis due to the variation in energy consumption, diesel had the highest sensitivity among the energies inputs. If diesel consumption is reduced by 10%, it could be estimated that $CO_2$ potential reduction is about 2.5%. When application rate of compound fertilizer reduces by 10%, the potential reduction is calculated to be approximately 1% for $CO_2$ and approximately 1.8% for $N_2O$. When drainage duration is decreased until 10 days, methane emissions is reduced by approximately 4.5%. That is to say drainage days, tillage, and reducing diesel consumption were the main sources having the largest effect of GHG reduction due to changing amount of inputs. Accordingly, proposed methods to decrease GHG emissions were no-tillage, midsummer drainage, etc.