• Title/Summary/Keyword: green weight

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Increased antioxidative effects of Seolitae Chungkukjang added with green tea powders (녹차 첨가 서리태 청국장의 항산화 증진 효과)

  • Cho, Eun-Ju;Park, Hyun-Young;Kim, Hyun-Young
    • Korean Journal of Agricultural Science
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    • v.39 no.1
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    • pp.75-79
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    • 2012
  • To increase antioxidative activity and minimize off-flavor of Chungkukjang, Seoliate as main ingredient and green tea as minor ingredient were selected. The protective efffect of green tea for Chungkukjang was evaluated under in vitro. Seolitae Chungkukjang added with green tea (SCG) was prepared on the basis of the protective effect of green tea from oxidative stress. Green tea showed the strong protective effect against nitric oxide (NO) and superoxide anion ($O_2{^-}$) radicals. The SCG perparation method was as follows. Seolitae was cleaned and washed, soaked in 1.5-fold water ($15^{\circ}C$) of Seolitae weight for 48 hr. Seolitae was steamed about 40 min by autoclave and then cooled about $40^{\circ}C$. Bacillus subtilis was inoculated and 5.0-fold green tea of weight was added, and then it was fermented for 60 hr at $40^{\circ}C$. According to the preparation method, Seolitae Chungkukjang (SC) and Seolitae Chungkukjang added with green tea 5.0% (SCG5.0) were prepared. Among the Chungkukjang groups SCG5.0 exerted more effective scavenging activity of NO than SC. In addition, SC added with green tea 5.0% showed high $O_2{^-}$ scavenging effect. These results suggest the antioxidative potential against free radical-induced oxidative damage of SCG.

Enzymatic Production of High Molecular Weight Chitooligosaccharides Using Recombinant Chitosanase from Bacillus thuringiensis BMB171

  • Kang, Lixin;Jiang, Sijing;Ma, Lixin
    • Microbiology and Biotechnology Letters
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    • v.46 no.1
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    • pp.45-50
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    • 2018
  • The chitosanase gene (btbchito) of Bacillus thuringiensis BMB171 was cloned and heterologously expressed in the yeast Pichia pastoris. After purification, about 300 mg of recombinant chitosanase was obtained from the 1-1 culture medium with a specific activity of 240 units/mg. Results determined by the combined use of thin layer chromatography (TLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) showed that the chitooligosaccharides (COSs) obtained by chitosan (N-deacetylated by 70%, 80%, and 90%) hydrolysis by rBTBCHITO were comprised of oligomers, with degrees of polymerization (DP) mainly ranging from trimers to heptamers; high molecular weight chitopentaose, chitohexaose, and chitoheptaose were also produced. Hydrolysis products was also deduced using MS since the COSs (n) are complex oligosaccharides with various acetyl groups from one to two, so the non-acetyl COSs (GlcN)n and COSs with more acetyls (> 2) were not detected. The employment of this method in the production of high molecular weight COSs may be useful for various industrial and biological applications, and the activity of chitosanase has great significance in research and other applications.

Effects of herbal acupuncture (Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) in obese Rats induced by high fat diet (마황(麻黃), 마황합록다(麻黃合綠茶) 약침(藥鍼)이 고지혈증(高脂血症) 유발(誘發) 백서(白鼠)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Kang, Ja-Don;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.21 no.1
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    • pp.51-60
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    • 2004
  • Objectives : This research was performed to investigate the effect of herbal acupuncture(Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) on weight gain, food intakes, food efficiency ratios, HDL-cholesterol, triglyceride, free fatty acid on rats fed high fat diet for 5weeks. Methods : Experimental groups were divided into normal group(Sham), high fat diet group(Con), high fat diet and Ephedra sinica-herbal acupuncture in SP9 ST40 treated group(ES), high fat diet and Green tea-herbal acupuncture in SP9 ST40 treated group (GT), high fat diet and Ephedra sinica+Green tea-herbal acupuncture in SP9 ST40 treated group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 19.125mg/ml per 2 days during 5 weeks. Results : Body weight, feed intake and feed efficiency ratio were decreased in Sham, ES, ES+GT, compared with control group. The level of serum HDL-cholesterol was increased in sham, tender to increase in ES, ES+GT. The change on that of serum triglyceride was decreased in sham, ES, ES+GT and free fatty acid was decrease in sham, ES+GT. Conclusions : Ephedra sinica with Green tea-herbal acupuncture in SP9 ST40 can control the Body weight, feed intake and feed efficiency ratio, HDL-cholesterol, triglyceride, free fatty acid.

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The effects of light colour on female rabbit reproductive performance and the expression of key genes in follicular development

  • Xiaoqing, Pan;Xinglong, Wang;Le, Shao;Jie, Yang;Feng, Qin;Jian, Li;Xia, Zhang;Pin, Zhai
    • Journal of Animal Science and Technology
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    • v.64 no.3
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    • pp.432-442
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    • 2022
  • The purpose of this study was to analyse the effects of light colour on rabbit reproductive performance and the expression of key follicular development genes. Rabbits (n = 1,068, 5 months old, 3.6-4.4 kg live body weight) were divided randomly into four groups, housed individually in wire mesh cages and exposed to red, green, blue, and white light-emitting diode (LED) light (control). The lighting schedule was 16 L : 8 D-15 d / 150 lx / 6:00 am-22:00 pm (3 d preartificial insemination to 12 d postartificial insemination). Red light and white light affected the conception rate and kindling rate and increased the total litter size at birth (p < 0.05). The effects of red light on litter size at weaning, litter weight at weaning, and individual weight at weaning increased compared with the green and blue groups. The effects of red light on live litter size at birth were increased compared with those in the blue group (p < 0.05). Compared to white light, green and blue light reduced the number of secondary follicles (p < 0.05). Compared to red light, green and blue light reduced the number of tertiary follicles (p < 0.05). Compared with white light, red LED light resulted in greater ovarian follicle stimulating hormone receptor and luteinizing hormone receptor mRNA expression (p < 0.05). Compared with green and blue LED light, red LED light resulted in greater B-cell lymphom-2 mRNA expression (p < 0.05). Compared with green LED light, red LED light inhibited FOXO1 mRNA expression in rabbit ovaries (p < 0.05). Red light can affect the reproductive performance of female rabbits and the expression of key genes for follicular development.

Evaluation on Adaptation of Zosia japonica as Effected by Different Green Roof System under Rainfed Conditon (무관수 옥상녹화시스템의 차이에 따른 들잔디 적응성 평가)

  • Ju, Jin-Hee;Kim, Won-Tae;Choi, Woo-Young;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.19 no.9
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    • pp.1137-1142
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    • 2010
  • This study proposes a guideline of a green roof system suitable for the local environment by verifying the growth of Zoysia japonica in a shallow, extensive, green roof system under rainfed condition. The experimental soil substrates into which excellent drought tolerance and creeping Z. japonica was planted were made with different soil thicknesses(15cm, 25cm) and soil mixing ratios(SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$). The plant height, green coverage ratio, fresh weight, dry weight and chlorophyll contents of Z. japonica were investigated. For the soil thickness of 15cm, the plant height of Z. japonica was significantly as affected by the soil mixing ratio and it was shown in the order SL= $P_4P_4L_2$ < $P_7P_1L_2$ = $P_5P_3L_2$ < $P_6P_2L_2$. For the soil thickness of 25cm, the plant height was increased in order to SL < $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ < $P_4P_4L_2$. The green coverage ratio was not observed by soil the mixing ratio or soil thickness. However, the green coverage ratio was 86~90% with a good coverage rate overall. The chlorophyll contents of Z. japonica were not significantly affected by the soil mixing ratio in the soil thickness of 15cm, but were higher in the natural soil than in the artificial soil at 25cm soil thickness. The fresh weight and dry weight of Zoysia japonica were heavier in the 25cm thickness than in the 15cm thickness and in the artificial soil mixture than in the natural soil. The result indicated that the growth of Zoysia japonica was more effective in the 25cm soil thickness with artificial soil than in the 15cm soil thickness with natural soil in the green roof system under rainfed condition.

Biochemical Studies on Colon Tumor Non-Promoting Effect of Green Tea Extract in Chemical Carcinogen-Treated Rats (화학적 발암원을 투여한 쥐에서 녹차 추출물의 대장암 억제효과에 관한 생화학적 연구)

  • 박현서
    • Journal of Nutrition and Health
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    • v.33 no.6
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    • pp.632-638
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    • 2000
  • This study was designed to observe the effect of green tea on colon tumor incidence and biomarkers of colon carcinogenesis in 1, 2-dimethlhydrazine-treated rats. Male Sprague Dawley rats at 7 weeks of age were divided into two groups: control and green tea(GT) groups. Control rats had distilled water as drinking water but GT group received green tea extracts(2.5%, w/v water) as drinking water throughout the experiment periods. All rats were fed the experimental diet containing 15% fat by weight for 20 weeks. and were i.m. injected with DMH for 6 weeks to give total dose of 180mg/kg body weight. Tumor incidence was reduced in GT group (39%) compared with control group (56%) Green tea significantly reduced cell proliferation (total cells per crypt, crypt length and proliferative zone) in colonic mucosa and also significantly reduced the levels of preformed prostalandin E2(PGE2) and thromboxance B2(TXB2) in colonic mucosa but the fatty acid profile of total lipid in colonic mucosa was not significantly influenced by green tea. However the relative percent of C20:4 and the levels f preformed PGE2 and TXB2. were significantly higher in tumor tissue compared with normal surrounding mucosa.Green tea increased the fecal excretion of total bile acid but not scondary bile acid which is known as one of promoters for colon cancer,. These results suggest that green tea could have preventive effect against colon cancer when consumed daily by influencing on antioxidant effect and the metabolism of arachidonic acid.

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A Study on Green Management of Petroleum Refining and Chemical Companies Applying to Assessment Indicator of Green Management (녹색경영 평가지표를 적용한 국내·외 정유/화학기업의 녹색경영 비교 연구)

  • Paik, Jang Hyun
    • Journal of Climate Change Research
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    • v.3 no.2
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    • pp.101-116
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    • 2012
  • Green management is, as the response to climate changes emerges to a global issue, a concept which reduction of greenhouse gases, alternative energy development and other low-carbon green growth factors are added on the existing environmental management. Also as discussions on the performance measurement of firms' domestic and international green managements proliferate and the importance of understanding, analyzing and evaluating firms' environmental maintenance rises, variety forms of green management performance measurement indicators are being developed and practiced. However, specific implementation methodologies about green management have been lacking. Hence in this research, In order to have objective evaluations on firms' green managements through the proposed indicator, AHP(Analytic Hierarchy Process) and experts' survey on weight dependence were applied. Sustainability reports and other associated documents of ten major oil/ chemical corporations of in and out of the country were analyzed and corporations were evaluated and compared through weight differentiated indicator subsections.

The Preference and Frequency of Beverages related to Health Factor in University Students (대학생들의 건강관련 요인이 커피와 전통음료의 기호도와 섭취빈도에 미치는 영향)

  • Shin, Sun-Young;Chung, La-Na
    • Journal of the Korean Society of Food Culture
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    • v.22 no.4
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    • pp.420-433
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    • 2007
  • This study was performed to investigate the effect of the health related factors on the preference and frequency of intake of coffee and traditional beverages among 280 university students (128 males and 152 females) who were residing in Incheon areas. The results were as follows; 1. Male students of over weight and obese were more than female students and female students of under weight were more than male students. Female students were interested in weight control and had an experience in weight control more than male students. 2. The group who was much interested in weight control preferred green tea, yuja tea and dunggulre tea. The group who was much interested in health when drink beverages preferred green tea, ginseng tea, dunggulre tea, vinegar drinks and water but didn’t prefer coffee. 3. The frequency of intake of green tea is high in the group who had an experience in weight control. And the frequency of intake of honey tea was high in the group who drank alcohol almost every day. Coffee intake was the highest among beverages in smokers and water intake was the highest among beverages in nonsmokers. In conclusion this study showed that as an interest in health is higher, the preference and frequency of intake of coffee was low whereas those who intake of traditional beverages was high in general.

Decolorization in Dyebath by Dye Adsorption of Chitin -Effects on the Adsorption in Direct Dyebath- (키틴의 염료 흡착에 의한 염액의 색도제거에 관한 연구 -직접염료의 염액을 중심으로-)

  • 유혜자;김정희;이혜자;이전숙
    • Textile Coloration and Finishing
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    • v.13 no.1
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    • pp.38-44
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    • 2001
  • The adsorption ability of dyes on chitin, a natural polymer was investigated for decolorization of dye wastewater. Chitin was manufactured in lab by decalcification in dilute aqueous hydrochloric acid solution and deproteination in dilute aqueous sodium hydroxide solution with shrimp shells. Absorbance of residue solution of dyebaths after dye adsorptions of chtin were measured in varieties of dye concentration and dipping periods. Three kinds of Direct dyes -C.I. Direct Red 81 (red 81), C.I. Direct Brown l(brown 1) and C.I. Direct Green 26(green 26) - were used. Red 81 and brown 1 have smaller molecular weight than green 26. The results from experimentals were of]tamed as follows: 1) Adsorption of green 26 was improved in dyebath by addition of salt, but red 81 and brown 1 were not found any improvements. 2) Smaller size of chitin particles could be absorbed much more speedy. In this experiments, the smallest range of chitin particle size was $250\mu{m}$ and less. 3) The most efficient amount of chitin for 20m1 of dyebath was 0.2g, 4) Both of red 81 and brown 1 showed good and speedy adsorption abilities as dyestuffs of over 90 percent in just one minute in dyebath of 0.01% dye concentration. But green 26 was absorbed slowly because of its large molecular weight. It took 40minutes to absorb dyestuffs of over 90 percent in dyebath of 0.01% dye concentration.

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Meta-regression analysis for anti-diabetic effect of green tea (녹차의 항-당뇨 효과에 대한 메타회귀분석)

  • Yun, A-Reum;Choi, Ki-Heon
    • Journal of the Korean Data and Information Science Society
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    • v.22 no.4
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    • pp.717-726
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    • 2011
  • The present study was carried out to summarize the effect of green tea in the diabetic rats by meta-analysis related studies. The association measure to test effect of green tea was Hedges' standardized mean difference. In this particular fixed effect model, body weight was significantly increased. Also, blood glucose, triglycerides were significantly decreased. In this case of heterogeneous variable, random effect model was applied. In this model, body weight was significantly increased. Also, blood glucose was significantly decreased in green tea treated group. According to the Meta-regression analysis, duration of injection was not significant for variables.