• Title/Summary/Keyword: green method

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Identification and Antioxidant Activity using Electron Spin Resonance Spectrometry of Antioxidant Producing Marine Actinomycetes Streptomyces sp. ACT-18 (항산화물질을 생산하는 해양방선균 Streptomyces Sp. ACT-18의 동정 및 Electron Spin Resonance Spectrometry를 이용한 항산화활성)

  • Kim, Man-Chul;Kim, Ju-Sang;Harikrishnan, Ramasamy;Han, Yong-Jae;Heo, Moon-Soo
    • Microbiology and Biotechnology Letters
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    • v.38 no.1
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    • pp.24-31
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    • 2010
  • For the research of the natural marine antioxidant, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-18 was gram positive, aerobic, non-motile spores. Substrate mycelia are dark green and yellow gray aerial mycelia. The cell size of the strain was $0.5{\sim}1.0\;{\mu}m$. 16S rDNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces sp. Results of cellular fatty acid analysis showed that major cellular fatty acids were $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%) and $C_{17:0}$ anteiso (10.99%). The antioxidant activity of methanol extract from Streptomyce sp. ACT-18 was evaluated by measuring 1,1-diphenyl- 2-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME (Streptomyces Broth Methanol Extract) A-18 was 46% at 0.1 mg/mL. Hydroxyl radical scavenging activity of SBME A-18 was 63% at 0.1 mg/mL. Alkyl radical scavenging activity of SBME A-18 was 39% at 0.1 mg/mL.

Preparation of Natural Compounds-tripeptides Derivatives and Their Melanogensis Inhibitory Activity (천연유래물질 펩타이드 유도체의 제조와 멜라닌 생성 저해 효과 탐색)

  • Shin, Kyong-Hoon;Lee, Jae-Ho;Ryu, Geun-Seok;Jee, Kyung-Yup;Park, Soo-Nam;Kim, Jae-Il;Cho, In-Shik;Kim, Han-Young
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.3
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    • pp.233-240
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    • 2010
  • Derivatives of a novel natural compunds, melanostatin (PLG-$NH_2$) were prepared by solid phase synthesis[1,2] and assayed to evaluate their melanogensis inhibitory activity. Also, a small library (natural compound-XLG-$NH_2$, natural compound-X LG-OH) was prepared with same method for increasing synthetic yield and cost-reduction. PLG-$NH_2$ (Proline-Leucine-Glycine-$NH_2$) was well-known tripeptide as its $\alpha$-MSH release-inhibiting activity and tyrosinase inhibitory activity[3-5]. In order to choose best candidate for peptide derivatization, various natural compounds were screened by their tyrosinase inhibitory activity. As a result, caffeic acid and coumaric acid were selected. Most of these derivatives showed better activities than the parent natural compound, melanostatin.

Effect of Reaction Conditions for n-Butane Dehydrogenation over Pt-Sn/θ-Al2O3 Catalyst (Pt-Sn/θ-Al2O3 촉매상에서 반응조건에 따른 n-부탄의 탈수소화 반응)

  • Cho, Kyung-Ho;Kang, Seong-Eun;Park, Jung-Hyun;Cho, Jun-Hee;Shin, Chae-Ho
    • Clean Technology
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    • v.18 no.2
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    • pp.162-169
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    • 2012
  • Pt-Sn/${\theta}-Al_2O_3$ catalyst for n-butane dehydrogenation reaction was prepared by incipient wetness method. To confirm the physicochemical properties of Pt-Sn/${\theta}-Al_2O_3$ catalyst, the characterization was performed using X-ray diffraction (XRD), $N_2$ sorption analysis, temperature programmed desorption of $NH_3$ ($NH_3$-TPD), temperature programmed reduction of $H_2$ ($H_2$-TPR) techniques. Also, the catalytic activities of Pt-Sn/${\theta}-Al_2O_3$ for n-butane dehydrogenation was tested as a function of pretreatment temperature, pretreatment time, reaction temperature, and the partial pressure of n-butane and hydrogen. The sum of selectivities to n-butenes consisting of 1-butene, cis-2-butene, and trans-2-butene was almost constant 95% in the range of conversion of n-butane 5-55%. The activation energy calculated from Arrhenius equation was $82.4kJ\;mol^{-1}$ and the reaction orders of n-butane and hydrogen from Power's law were 0.70 and -0.20, respectively.

Development of Oxo-biodegradable Transparent Bio Films Using Biomass and Biodegradable Catalyst (바이오매스 및 생분해 촉매제를 이용한 산화생분해 투명 바이오 필름 개발)

  • You, Young-Sun;Kim, Young-Tae;Park, Dae-Sung;Choi, Sung-Wook
    • Clean Technology
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    • v.23 no.2
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    • pp.133-139
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    • 2017
  • Bio-based plastics containing the biomass content higher than 25 wt% have been considered as environment-friendly materials due to their effects on the reduction in the $CO_2$ emission and petroleum consumption as well as biodegradability after use. In this study, poly vinyl chloride, plant-derived plasticizers, by adding a biodegradable catalyst was observed a change in the biodegradability and physical properties. To produce the oxidative decomposition transparent bio film, which is broken down in the initial percent elongation and physical properties such as tensile strength, it was to test the safety of the product as a food packaging material. Poly vinyl chloride, primary plasticizer, secondary plasticizer, anti fogging agent, the combined stabilizer were mixed in a high speed mixer, then extruded using an extrusion molding machine, after cooling, winding, to produce a oxidative decomposition transparent bio film and the control film, with a thickness of $12{\mu}m$ through winder role. Mechanical properties tensile strength, elongation, and the maximum load elongation and biodegradation test. Transparent bio film produced by biodegradation catalyst is compared with the control film. Tensile strength and elongation of films were found to be no significant difference. Further, as a result of the biodegradation test for 45 days based on the ASTM D6954-04 method, biodegrability of film is 61.4%.

Evaluation of Structural Response of Cylindrical Structures Based on 2D Wave-Tank Test Due to Wave Impact (파랑충격력에 의한 원형실린더구조물의 구조응답평가)

  • Lee, Kangsu;Ha, Yoon-Jin;Nam, Bo Woo;Kim, Kyong-Hwan;Hong, Sa Young
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.33 no.5
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    • pp.287-296
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    • 2020
  • The wave-impact load on offshore structures can be divided into green-water and wave-slamming impact loads. These wave impact loads are known to have strong nonlinear characteristics. Although the wave impact loads are dealt with in the current classification rules in the shipping industry, their strong nonlinear characteristics are not considered in detail. Therefore, to investigate these characteristics, wave-impact loads induced by a breaking wave on a circular cylinder were analyzed. A model test was carried out to measure the wave-impact loads due to breaking waves in a two-dimensional (2D) wave tank. To generate a breaking wave, the focusing wave method was applied. A series of 2D tank tests under a horizontal wave impact was carried out to investigate the structural responses of the cylindrical structure, which were obtained from the measured model test data. According to the results, we proposed a structural damage-estimation procedure of an offshore tubular member due to a wave impact load. Furthermore, a recommended wave-impact load is suggested that considers the minimum required thickness of each member. From the experimental results, we found that the required minimum thickness is dependent on the impact pressure located in a three-dimensional space on the surface of a tubular member.

Synthesis and Properties of SrMoO4 Phosphors Doped with Various Rare Earth Ions for Anti-Counterfeiting Applications (위조 방지 분야에 응용 가능한 다양한 희토류 이온이 도핑된 SrMoO4 형광체의 제조 및 특성)

  • Moon, Tae-Ok;Jung, Jae-Yong;Cho, Shinho
    • Korean Journal of Materials Research
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    • v.30 no.8
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    • pp.406-412
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    • 2020
  • SrMoO4:RE3+ (RE=Dy, Sm, Tb, Eu, Dy/Sm) phosphors are prepared by co-precipitation method. The effects of the type and the molar ratio of activator ions on the structural, morphological, and optical properties of the phosphor particles are investigated. X-ray diffraction data reveal that all the phosphors have a tetragonal system with a main (112) diffraction peak. The emission spectra of the SrMoO4 phosphors doped with several activator ions indicate different multicolor emissions: strong yellow-emitting light at 573 nm for Dy3+, red light at 643 nm for Sm3+, green light at 545 nm for Tb3+, and reddish orange light at 614 nm for Eu3+ activator ions. The Dy3+ singly-doped SrMoO4 phosphor shows two dominant emission peaks at 479 and 573 nm corresponding to the 4F9/26H15/2 magnetic dipole transition and 4F9/26H13/2 electric dipole transition, respectively. For Dy3+ and Sm3+ doubly-doped SrMoO4 phosphors, two kinds of emission peaks are observed. The two emission peaks at 479 and 573 nm are attributed to 4F9/26H15/2 and 4F9/26H13/2 transitions of Dy3+ and two emission bands centered at 599 and 643 nm are ascribed to 4G5/26H7/2 and 4G5/26H9/2 transitions of Sm3+. As the concentration of Sm3+ increases from 1 to 5 mol%, the intensities of the emission bands of Dy3+ gradually decrease; those of Sm3+ slowly increase and reach maxima at 5 mol% of Sm3+ ions, and then rapidly decrease with increasing molar ratio of Sm3+ ions due to the concentration quenching effect. Fluorescent security inks based on as-prepared phosphors are synthesized and designed to demonstrate an anti-counterfeiting application.

Change of Binocular Vision Induced by Longitudinal Chromatic Aberration during Near Work (근거리 작업 시 종색수차에 따른 양안시의 변화)

  • Kim, Se-il;Park, Mijung;Kim, So Ra
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.219-228
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    • 2015
  • Purpose: The current study was performed to compare the difference in binocular visual function depending on variable background colors at near work. Method: Fifty four adults (18 males, 34 females) who consented to the present study and had no ocular disease, ocular surgery history, strabismus and amblyopia with normal binocular vision were participated into this study. The subjects were asked to read the novels with black letter printed on white, red, green and blue background for 15 min. Then, their heterophoria, AC/A ratio, near point of convergence, accommodation facility, relative accommodation and vergence were measured before and after reading. The difference of measurements were compared. Result: Overall heterophoria was tended to decrease with regardless of background color. AC/A ratio showed a tendency of increase after reading the novels with all backgrounds except white background. Near point of convergence was significantly increased compared to before reading at all background color. Accommodative facility of dominant and non-dominant eyes were also significantly increased after reading however, binocular accommodative facility showed a tendency of decrease. Negative relative accommodation also decreased at all background colors however, the change of positive relative accommodation was not significantly different. In case of vergence, there was significant difference in break point of far BO and recovery point of far BI by the wavelength of background color. Conclusions: From the results, it was known there is convergence change depending on the wavelength of light even though same amount of accommodation and convergence is required when doing near work for certain period. Thus, it can be suggested that the adjustment of the near working environment which perception of various color was required, should be conducted according to the main wavelength.

Synthesis and Charaterization of Polymerizable Acryl's Emulsifier to prepare Green Glue (친환경 접착제 제조용 아크릴계 반응성 유화제의 합성)

  • Jeong, Noh-Hee;Park, Jong-Kwon;Kang, Yun-Seok
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.1
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    • pp.51-57
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    • 2016
  • In this study, we synthesized reactive surfactant which have functional radical group for effective chemical reaction. Reactive surfactant have been synthesized using synthesis of polyoxyethylene lauryl ether(POE 23) which is nonionic surfactant and methacrylic acid, acrylic acid. benzene was used as the solvent, p-TsOH was used as the catalyst. synthesized surfactant was confirmed by FT-IR, $^1H$-NMR spectra, and elemental analysis. Evaluation of physical properties was measured HLB, cloud point, surface tension, the critical micelle concentration, emulsifying power. HLB number was evaluated 11.62 to 12.09 range. The Critical Micelle Concentration(cmc) values evaluated was $1{\times}10^{-4}{\sim}5{\times}10^{-4}mol/L$ by surface tension method. The cloud point was $35^{\circ}C$, $39^{\circ}C$ each. The emulsifymvcqa ing properties of the synthesized surfactants was lower than polyoxyethylene lauryl ether. In addition, soybean oil was better than benzene. The experimental results confirmed the ester bond, the yield of 93.27%, 94.49% was found.

Strategies to Increase Domestic Lettuce Circulations through Improving Valuable End-User Traits (고부가가치 맞춤형 상추품종 개발을 통한 국내 상추유통 제고 전략)

  • Kim, Tae-Sung;Jang, Young-Hee;Hwang, Hee-Joong
    • Journal of Distribution Science
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    • v.16 no.8
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    • pp.63-68
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    • 2018
  • Purpose - Lettuce (Lactuca sativ L.) is one of the economically important vegetable crops, which worldwide market value is over 100 billion U.S. dollar. In Korea, about 89.7 kilo ton of lettuce was produced in 3400ha in 2016, recoded as No. 1 vegetable crop in domestic green house production. However, recently, domestic lettuce production and cultivation areas are all getting decreased. Thus, novel approaches are needed to be implemented to revive the production. Research design, data and methodology - In this review paper, we first prioritized the end-user traits which are imperative to positively stimulate the domestic lettuce market and discussed relevant genomics strategies. Especially, we assessed a possibility whether school meal program would be a potential niche market. Results - The genomics technologies, which become widely applied in the crop biotechnology since 2008 when next generation sequencing method was developed, may be a good solution in the crop improvement, efficiently gathering valuable information of agriculturally useful traits. Significantly, in lettuce, the high quality whole genome sequence, based on Lactuca sativa cv. Salinas, is publically available and this genomics platform, thus, would be implemented in lettuce breeding program to innovate relevant end-user traits both for the farmers and customers, including the disease resistance to the Fusarium wilt, productivity under hot weather conditions, various nutritional qualities and so forth. These improvements will boost domestic lettuce industries in the near future. Conclusions - Due to the nutritional distinctions comparing to the western style lettuces, domestic leaf lettuces could be one of the important vegetables in the school meal programs. To make it happen, we would better devise diverse recipes to make a salad with it, instead of only using as a wrap vegetable. Meanwhile, novel lettuce varieties need to be developed, which are favorable to the students and also easy to be handled with while processing. Overall, to achieve international competence in the lettuce industries, we need to create elite lettuce varieties that satisfies domestic farmers as well as customers, suitable to various niche markets, such as school meal program. Thus, efficient breeding programs using genomics approaches should be established in advance and careful monitoring on the preference of the related customers for a niche market be continued persistently.

Bactericidal Effect of Pathogenic Bacteria on Acid Treatment Combined with Red, Green, and Blue LED Light at a Low Temperature Environment (저온에서 산 처리와 적색, 녹색, 청색 LED 조사의 조합에 따른 식중독 세균의 살균 효과)

  • Do, Jung Sun;Chung, Hyun-Jung;Bang, Woo-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.11
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    • pp.1725-1732
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    • 2015
  • The bactericidal effects of 642, 521, and 461 nm LED were investigated on Escherichia coli O157:H7 and Staphylococcus aureus strains in TSB with pH 7.2, 4.0, and 3.5 for 10 h at $15^{\circ}C$. The bactericidal effect of 461 nm blue LED was the most pronounced compared to 642 nm and 521 nm LEDs at pH 3.5. When E. coli was exposed to pH 3.5 with 461 nm LED, populations of E. coli O157:H7 ATCC 43894 and 35150 decreased by 4 and 5 log CFU/mL for 2 h, respectively. Populations of E. coli ATCC 8739 decreased by 5 log CFU/mL for 2 h. Further, S. aureus ATCC 27664, 43300, and 19095 were inactivated by 4, 5 and 5 log CFU/mL for 2 h, respectively, at pH 3.5 with 461 nm LED. In conclusion, combined treatment with 461 nm LED and acidic conditions at low-temperature ($15^{\circ}C$) showed the greatest antimicrobial effects. This study suggests that LEDs may be potentially used as a method to maintain the safety of the food preservation technology.