• 제목/요약/키워드: green fusion technology

검색결과 80건 처리시간 0.027초

Improvement of Virus Safety of an Antihemophilc Factor IX by Virus Filtration Process

  • Kim, In-Seop;Choi, Yong-Woon;Kang, Yong;Sung, Hark-Mo;Sohn, Ki-Whan;Kim, Yong-Sung
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권7호
    • /
    • pp.1317-1325
    • /
    • 2008
  • Viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. One potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. In order to increase the viral safety of human antihemophilic factor IX, particularly in regard to non-enveloped viruses, a virus removal process using a polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. The most critical factor affecting the filtration efficiency was operating pH and the optimum pH was 6 or 7. Flow rate increased with increasing operating pressure and temperature. Recovery yield in the optimized production-scale process was 96%. No substantial changes were observed in the physical and biochemical characteristics of the filtered factor IX in comparison with those before filtration. A 47-mm disk membrane filter was used to simulate the process performance of the production-scale cartridges and to test if it could remove several experimental model viruses for human pathogenic viruses, including human hepatitis A virus (HAV), porcine parvovirus (PPV), murine encephalomyocarditis virus (EMCV), human immunodeficiency virus type 1 (HIV), bovine viral diarrhea virus (BVDV), and bovine herpes virus (BHV). Non-enveloped viruses (HAV, PPV, and EMCV) as well as enveloped viruses (HIV, BVDV, and BHV) were completely removed during filtration. The log reduction factors achieved were $\geq$6.12 for HAV, $\geq$4.28 for PPV, $\geq$5.33 for EMCV, $\geq$5.51 for HIV, $\geq$5.17 for BVDV, and $\geq$5.75 for BHV. These results indicate that the virus filtration process successfully improved the viral safety of factor IX.

녹색도로의 열 에너지 하베스팅을 위한 시스템 개발에 관한 연구 (A Study on the Development of Green Road System for Heat Energy Harvesting)

  • 조병완;이덕희;이동윤;이창섭
    • 한국도로학회논문집
    • /
    • 제13권1호
    • /
    • pp.87-96
    • /
    • 2011
  • 대량의 화석연료 소비로 인한 지구 생태계의 부작용에 따라 전 세계적으로 저탄소와 지속가능한 신재생에너지의 활용이 요구되고 있다. 본 논문에서는 여름철 아스팔트 포장도로에서 발생하는 열에너지를 열전현상을 이용하여 전기에너지로 변환하여 에너지 위기를 새로운 성장의 기회로 전환하려 하였다. 이를 위해 열발전소자의 전기적 특성실험과 아스팔트 포장도로 시험체에 열발전소자 매입 및 열 회수 파이프 매입 실험을 통해 에너지 하베스팅 전력을 측정하여 가장 효과적인 아스팔트 포장도로의 열에너지 하베스팅을 위한 시스템 구축방안을 검토하였으며, 결국 아스팔트 포장도로에서 발생하는 지속가능한 열 에너지원을 활용함과 동시에 환경보존과 경제성장을 도모할 수 있는 신개념의 녹색도로 사회기반시설을 제안하였다.

Li4SiO4 slurry conditions and sintering temperature for fabricating Li4SiO4 pebbles as tritium breeders for nuclear-fusion reactors

  • Young Ah Park;Ji Won Yoo;Yi-Hyun Park;Young Soo Yoon
    • Nuclear Engineering and Technology
    • /
    • 제55권8호
    • /
    • pp.2966-2976
    • /
    • 2023
  • A tritium breeder is a lithium-based material capable of producing tritium. Many researchers designing nuclear-fusion energy are studying tritium production using pebbles, which are solid-type breeders. The sphericity and size of the pebbles are critical in obtaining pebbles with good tritium-breeding efficiency. Furthermore, tritium-release efficiency can be increased by using pebbles with appropriate porosities. Promising raw materials for tritium-breeding materials include Li4SiO4 and Li2TiO3. Li4SiO4 has a higher lithium density than Li2TiO3 and exhibits excellent tritium-breeding efficiency. However, it has the disadvantage of being easily decomposed during the Li4SiO4-green-pebble sintering process because of its low structural stability at high temperatures and high lithium density. In this study, we attempted to determine the optimal conditions for manufacturing Li4SiO4 pebbles using the droplet-freeze-drying method. The optimal Li4SiO4 slurry conditions and sintering temperatures were determined. The optimal Li4SiO4 slurry-fabrication conditions were 3 wt% polyvinyl alcohol and 75 wt% Li4SiO4 based on the deionized-water weight content. The sintering temperature at which Li4SiO4 did not decompose and exhibited the optimum porosity of 10.8% was 900 ℃.

도시공원 유치거리를 고려한 녹지취약지역 분석 - 대구광역시 남구를 대상으로 - (Analysis on Green Vulnerable Areas Considering the Catchment Area of Urban Parks - Focused on Nam-gu in Daegu -)

  • 엄정희;이윤구
    • 한국지리정보학회지
    • /
    • 제19권2호
    • /
    • pp.117-131
    • /
    • 2016
  • 본 연구는 근린생활권 측면에서의 녹지취약지역을 개선할 수 있는 방안을 모색해보고자 대구광역시 남구에 위치한 공원를 대상으로 공원별 법적 유치거리를 기준으로 도시공원의 녹지서비스 공급지역과 녹지서비스 취약지역을 분석하였다. 또한, 녹지공간을 조성할 수 있는 후보지역을 선정하고 이를 기준으로 녹지서비스 공급지역을 분석하여, 향후 녹지서비스 취약지역의 개선 가능성을 제시하였다. 행정구역 대비 서비스 면적률을 비교하였을 때, 현재 조성되어 있는 16개 공원에 의한 녹지서비스가 충분한 지역은 대명6동(94.4%) 및 대명5동(92.3%)이었고, 녹지서비스가 취약한 지역은 이천동(1.6%)과 대명10동(24.1%)으로 분석되었다. 하지만, 남구에 계획되어있는 12개의 미집행공원이 조성된다면 이천동의 녹지서비스 면적률이 88.6%로 향상되고, 관공서와 학교를 중심으로 녹지공간이 추가로 조성된다면 대명10동 역시 녹지서비스 면적률이 40.1%로 향상되는 것으로 분석되었다. 본 연구의 결과는 도시공원의 접근성에 따른 형평성을 고려하는 녹지정책을 수립하기 위한 기초자료로 활용될 수 있을 것으로 판단된다.

환경수업에 대한 예비초등교사의 인식 (Recognition of Korean Pre-service Elementary Teachers Concerning Environment Class)

  • 김동욱;김성훈
    • 한국환경과학회지
    • /
    • 제23권8호
    • /
    • pp.1507-1512
    • /
    • 2014
  • Recognition of Korean pre-service elementary school teachers concerning the factor for the environmental class was investigated and analyzed by the factorial analysis. Korean pre-service elementary school teachers are aware of the factor of 'participation in environment' as well as the factor of 'environmental knowledge' for the environmental class. Furthermore, they had a very positive attitude about the factor of 'participation in environment'.

Dry-Heat Treatment Process for Enhancing Viral Safety of an Antihemophilic Factor VIII Concentrate Prepared from Human Plasma

  • Kim, In-Seop;Choi, Yong-Woon;Kang, Yong;Sung, Hark-Mo;Shin, Jeong-Sup
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권5호
    • /
    • pp.997-1003
    • /
    • 2008
  • Viral safety is a prerequisite for manufacturing clinical antihemophilic factor VIII concentrates from human plasma. With particular regard to the hepatitis A virus (HAV), a terminal dry-heat treatment ($100^{\circ}C$ for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor VIII concentrate. The loss of factor VIII activity during dry-heat treatment was of about 5%. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor VIII compared with those of the factor VIII before dry-heat treatment. The dry-heat-treated factor VIII was stable for up to 24 months at $4^{\circ}C$. The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV, murine encephalomyocarditis virus (EMCV), and human immunodeficiency virus (HIV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Bovine herpes virus (BHV) and bovine viral diarrhea virus (BVDV) were potentially sensitive to the treatment. However porcine parvovirus (PPV) was slightly resistant to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were ${\geq}5.55$ for HAV, ${\geq}5.87$ for EMCV, ${\geq}5.15$ for HIV, 6.13 for BHV, 4.46 for BVDV, and 1.90 for PPV. These results indicate that dry-heat treatment improves the virus safety of factor VIII concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid-enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment.

사파이어와 석영 기판 위에 성장된 SrSnO3:Tb3+ 녹색 형광체 박막의 특성 (Properties of SrSnO3:Tb3+ Green-Emitting Phosphor Thin Films Grown on Sapphire and Quartz Substrates)

  • 조신호
    • 한국전기전자재료학회논문지
    • /
    • 제29권9호
    • /
    • pp.546-551
    • /
    • 2016
  • $SrSnO_3:Tb^{3+}$ phosphor thin films were prepared on sapphire and quartz substrates in the growth temperature range of $100{\sim}400^{\circ}C$ by using the radio frequency magnetron sputtering deposition. The resulting $SrSnO_3:Tb^{3+}$ thin films were characterized by X-ray diffraction, scanning electron microscopy, ultraviolet-visible-infrared spectrophotometer, and photoluminescence spectrometer. The results indicated that the morphology, optical transmittance, band gap energy, and luminescence intensity of the phosphor thin films significantly depended on the growth temperature. All the thin films, regardless of the type of substrate, showed an amorphous behavior. As for the thin films deposited on sapphire substrate, the maximum crystallite size was obtained at a growth temperature of $400^{\circ}C$ and the strongest emission was green at 544 nm arising from the $^5D_4{\rightarrow}^7F_5$ transition of Tb3+. The average optical transmittance for all the thin films grown on sapphire and quartz substrates was decreased as the growth temperature increased from 100 to $400^{\circ}C$. The results suggest that the optimum growth temperatures for depositing highly-luminescent $SrSnO_3:Tb^{3+}$ phosphor thin films on sapphire and quartz substrates are 400 and $300^{\circ}C$, respectively.

A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins

  • Kim, Yun-Kyoung;Oh, Soo-Jin;Jin, Bong-Suk;Park, Chan-Hoo;Jeon, Hye Sung;Boo, Doo-Wan;Yu, Yeon-Gyu
    • Bulletin of the Korean Chemical Society
    • /
    • 제30권3호
    • /
    • pp.577-581
    • /
    • 2009
  • Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-$\gamma$. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.

In Vitro Selection of High Affinity DNA-Binding Protein Based on Plasmid Display Technology

  • Choi, Yoo-Seong;Joo, Hyun;Yoo, Young-Je
    • Journal of Microbiology and Biotechnology
    • /
    • 제15권5호
    • /
    • pp.1022-1027
    • /
    • 2005
  • Based on plasmid display technology by the complexes of fusion protein and the encoding plasmid DNA, an in vitro selection method for high affinity DNA-binding protein was developed and experimentally demonstrated. The GAL4 DNA-binding domain (GAL4 DBD) was selected as a model DNA-binding protein, and enhanced green fluorescent protein (EGFP) was used as an expression reporter for the selection of target proteins. Error prone PCR was conducted to construct a mutant library of the model. Based on the affinity decrease with increased salt concentration, mutants of GAL4 DBD having high affinity were selected from the mutant protein library of protein-encoding plasmid complex by this method. Two mutants of (Lys33Glu, Arg123Lys, Ile127Lys) and (Ser47Pro, Ser85Pro) having high affinity were obtained from the first generation mutants. This method can be used for rapid in vitro selection of high affinity DNA-binding proteins, and has high potential for the screening of high affinity DNA-binding proteins in a sequence-specific manner.

새로운 스트레스 단백질인 VISP의 세포내 위치 (Subcellular Localization of Novel Stress Protein VISP)

  • 문창훈;윤원준;고명석;김현주;박정우
    • 미생물학회지
    • /
    • 제42권4호
    • /
    • pp.271-276
    • /
    • 2006
  • 이전의 연구 결과 어류 rhabdovirus에 감염된 세포에서 virus-inducible stress protein (VISP)의 발현이 증가함을 확인하였다. 본 연구에서는 VISP의 세포내 위치를 확인하였으며, 또한 세포내 위치 결정에 중요한 역할을 담당하는 VISP의 부위를 확인하였다. 먼저 endogenous VISP의 세포내 위치를 확인하기 위하여 CHSE-214 세포를 VISP에 대한 단클론항체를 사용하여 염색한 후 confocal microscope로 관찰하였다. 그 결과 VISP가 세포의 핵 주변에 점구조를 형성함이 확인되었다. 이를 확인하기 위하여 VISP에 enhanced green fluorescent protein (EGFP)이 붙은 fusion gene을 발현하는 plasmid를 제조하였다. EGFP-VISP를 발현하는 plasmid 벡터를 세포에 transfection 시킨 후 confocal microscope로 관찰한 결과 핵 주변에 점구조를 형성함이 확인되었다. VISP의 아미노산서열 중 핵 주변의 점구조 형성에 관여하는 부분을 확인하기 위하여 VISP의 다양한 deletion mutant들을 제조하였다. 이 mutant를 사용한 transfection 실험 결과 VISP의 C-terminal 부위(aa 612-710)가 핵주변의 점구조 형성에 중요한 역할을 담당함이 확인되었으며, 이 부분의 functional motif 분석결과 691-TLTSLLL-697 부위에 nuclear receptor binding motif가 존재함이 확인되었다. 이와 같은 결과들을 종합하면, VISP는 핵 주변에 존재하며 VISP의 C-terminal부위가 혀 주위 분포에 중요한 역할을 담당함을 알 수 있었다. 이후의 연구로부터 VISP의 핵 주위 분포가 IHNV의 성장에 미치는 영향이 확인되면 IHNV 병원성의 새로운 기작을 밝혀내는 중요한 자료가 될 것이다.