Browse > Article
http://dx.doi.org/10.5012/bkcs.2009.30.3.577

A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins  

Kim, Yun-Kyoung (Department of Chemistry, Kookmin University,Division of Life Science, Korea Institute of Science and Technology)
Oh, Soo-Jin (Division of Life Science, Korea Institute of Science and Technology)
Jin, Bong-Suk (Department of Chemistry, Kookmin University)
Park, Chan-Hoo (Department of Chemistry, Yonsei University)
Jeon, Hye Sung (Division of Life Science, Korea Institute of Science and Technology)
Boo, Doo-Wan (Department of Chemistry, Yonsei University)
Yu, Yeon-Gyu (Department of Chemistry, Yonsei University)
Publication Information
Bulletin of the Korean Chemical Society / v.30, no.3, 2009 , pp. 577-581 More about this Journal
Abstract
Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-$\gamma$. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.
Keywords
HBV; PreS; Capsid; Interaction;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Le Seyec, J.; Chouteau, P.; Cannie, I.; Guguen-Guillouzo, C.; Gripon, P. J. Virol. 1998, 72, 5573
2 Le Seyec, J.; Chouteau, P.; Cannie, I.; Guguen-Guillouzo, C.; Gripon, P. J. Virol. 1999, 73, 2052
3 Poisson, F.; Severac, A.; Hourioux, C.; Goudeau, A.; Roingeard, P. Virology 1994, 228, 115   DOI   ScienceOn
4 Tan, W. S.; Dyson, M. R.; Murray, K. J. Mol. Biol. 1999, 286, 797   DOI   ScienceOn
5 Stray, S. J.; Johnson, J. M.; Kopek, B. G.; Zlotnick, A. Nat. Biotechnol. 2006, 24, 358   DOI   ScienceOn
6 Bruss, V.; Ganem, D. Proc. Natl. Acad. Sci. USA 1991, 88, 1059   DOI   ScienceOn
7 Asif-Ullah, M.; Choi, K. J.; Choi, K. I.; Jeong, Y. J.; Yu, Y. G. Antiviral Res. 2006, 70, 85   DOI   ScienceOn
8 Choi, I.: Yu, Y. G. Infec. Dis. Drug Targets 2007, 7, 251   DOI   ScienceOn
9 Bruss, V.; Thomssen, R. J. Virol. 1994, 68, 1643
10 Eble, B. E.; MacRae, D. R.; Lingappa, V. R.; Ganem, D. Mol. Cell Biol. 1987, 7, 3591
11 Bruss, V.; Vieluf, K. J. Virol. 1995, 69, 6652
12 Glebe, D.; Urban, S. World J. Gastroenterol. 2007, 13, 22   DOI
13 Prange, R.; Streeck, R. E. EMBO. J. 1995, 14, 247
14 Patzer, E. J.; Nakamura, G. R.; Simonsen, C. C.; Levinson, A. D.; Brands, R. J. Virol. 1986, 58, 884
15 Loffler-Mary, H.; Dumortier, J.; Klentsch-Zimmer, C.; Prange, R. Virology 2000, 270, 358   DOI   ScienceOn
16 Yamamoto, M.; Hayashi, N.; Takehara, T.; Ueda, K.; Mita, E.; Tatsumi, T.; Sasaki, Y.; Kasahara, A.; Hori, M. Hepatology 1999, 30, 300   DOI
17 Bruss, V. J. Virol. 1997, 71, 9350
18 Choi, K. J.; Lim, C. W.; Yoon, M. Y.; Ahn, B. Y.; Yu, Y. G. Biochem. Biophys. Res. Commun. 2004, 319, 959   DOI   ScienceOn
19 Ponsel, D.; Bruss, V. J. Virol. 2003, 77, 416   DOI   ScienceOn
20 Butz, K.; Denk, C.; Fitscher, B.; Crnkovic-Mertens, I.; Ullmann, A.; Schroder, C. H.; Hoppe-Seyler, F. Oncogene 2001, 20, 6579   DOI   ScienceOn
21 Scaglioni, P.; Melegari, M.; Takahashi, M.; Chowdhury, J. R.; Wands, J. Hepatology 1996, 24, 1010   DOI   ScienceOn
22 von Weizsacker, F.; Kock, J.; Wieland, S.; Offensperger, W. B.; Blum, H. E. Hepatology 1999, 30, 308   DOI
23 Bottcher, B.; Tsuji, N.; Takahashi, H.; Dyson, M. R.; Zhao, S.; Crowther, R. A.; Murray, K. EMBO. J. 1998, 17, 6839   DOI   ScienceOn
24 Dyson, M. R.; Murray, K. Proc. Natl. Acad. Sci. USA 1995, 92, 2194   DOI   ScienceOn
25 Schodel, F.; Peterson, D.; Zheng, J.; Jones, J. E.; Hughes, J. L.; Milich, D. R. J. Biol. Chem. 1993, 268, 1332
26 Lemmon, M. A.; Ferguson, K. M.; O'Brian, R.; Sigler, P. B.; Schlessinger, J. Proc. Natl. Acad. Sci. USA 2006, 92, 10472   DOI   ScienceOn
27 Stauffer, T. P.; Ahn, S.; Meyer, T. Curr. Biol. 1998, 8, 343   DOI   ScienceOn
28 Vives, E.; Brodin, P.; Lebleu, B. J. Biol. Chem. 1997, 272, 16010   DOI   ScienceOn
29 Pumpens, P.; Borisova, G. P.; Crowther, R. A.; Grens, E. Intervirology 1995, 38, 63
30 Kratz, P. A.; Bottcher, B.; Nassal, M. Proc. Natl. Acad. Sci. USA 1996, 96, 1915   DOI   ScienceOn