Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins

  • Kim, Yun-Kyoung (Department of Chemistry, Kookmin University,Division of Life Science, Korea Institute of Science and Technology) ;
  • Oh, Soo-Jin (Division of Life Science, Korea Institute of Science and Technology) ;
  • Jin, Bong-Suk (Department of Chemistry, Kookmin University) ;
  • Park, Chan-Hoo (Department of Chemistry, Yonsei University) ;
  • Jeon, Hye Sung (Division of Life Science, Korea Institute of Science and Technology) ;
  • Boo, Doo-Wan (Department of Chemistry, Yonsei University) ;
  • Yu, Yeon-Gyu (Department of Chemistry, Yonsei University)
  • Published : 2009.03.20
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Abstract

Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-$\gamma$. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.

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