• The Plant Pathology Journal
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• v.36 no.2
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• pp.170-178
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• 2020

The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV-infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been developed to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65℃ and 60℃ for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction assays. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.183-187
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• 2014

Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMV-RoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of the LMV isolates contains two large subgroups, LMV-Common and LMV-Most. To date, however, no Korean LMV isolate has been classified and characterized. In this study, LMV-Muju, the Korean LMV isolate, was isolated from lettuce showing pale green and mottle symptoms, and its complete genome sequence was determined. Classification method of LMV isolates based on nucleotide sequence divergence of the NIb-CP junction showed that LMV-Muju was categorized as LMV-Common. LMV-Muju was more similar to LMV-O (LMV-Common subgroup) than to LMV-E (LMV-RoW group but not LMV-Common subgroup) even in the amino acid domains of HC-Pro associated with pathogenicity, and in the CI and VPg regions related to ability to overcome resistance. Taken together, LMV-Muju belongs to the LMV-Common subgroup, and is expected to be a seed-borne, non-resistance-breaking isolate. According to our analysis, all other LMV isolates not previously assigned to a subgroup were also included in the LMV-RoW group.

• Korean Journal of Organic Agriculture
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• v.15 no.4
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• pp.399-411
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• 2007

Korean organic farming has been well developed over the last two decades. It demonstrates that the number of certificated farm for organic agriculture and products have been drastically increased in recent year. However, the organic farmers have thought that organic farming rely only on organic fertilizer and they don't keep organic farming principle in which organic farmer should enhance biological activity and crop rotation. This study was to compare nutrient input, recommendation, cropping system and organic product circulation between the early $20^{th}$ century and beginning of the $21^{st}$ century. The population of Korea has increased 7.3 times more than that of a century ago but cultivated land has been decreased during 100 years. The rice production in 2002 was 4.2 times higher than that of production in 1912. The input of N, P and K in 1907 on the basis of King's suggestion was 95.6kg/ha, 15.9kg/ha and 3.0kg/ha, respectively. Nitrogen came from excreta (40%), green manure (55%) and compost (5%) in the early 20th century. On the other hand, organic farmer input organic resources such as wood chip (30.1%), compost (27.8%), rice straw (14%) and others (25%) these days. In terms of nutrient balance calculated nutrient and absorption by plants, organic rice farmer apply excessive nitrogen and phosphorus to the soil. They was used to put $7{\sim}10$ times more nitrogen than that of a century ago. Nutrient recommendation was similar in N and P between early 20th century and early $21^{st}$ century. Farmers in both century did not rotate crops in the field. Today, organic farmers engaged in more continuous cultivation than in early 20th century. Farmers in the early $20^{th}$ century produced locally, consumed locally the agricultural products, but organic farmers in the $21^{st}$ century produce the organic product in the local farmland and consumed in the large city and also a lot of foreign organic products have been imported in recent year.

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.181-187
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• 2005

For 4 years (2000 ${\sim}$ 2003), annual fluctuations of standing crops of cyanobacteria and physicochemical factors were examined at five sites from Bridge of Seungsoo to Bridge of Seungsan in the lower part of Han River. The cyanobacterial abundance (ND to 4,167 cells $mL^{-1}$) was strongly decreased during the heavy rains in every year. During the similar periods in 2003, cyanobacteria hardly observed, and comprised below of 10 percentage of total phytoplankton. In the period of little cyanobacteria, some green algae and diatom dominated the phytoplankton community, while the concentration of chlorophyll a has not largely change. These results indicate that heavy frequent precipitation strongly limited the growth of cyanobacteria, and lead an algal succession by the appearance of new algal groups.

• Magazine of the Korean Society of Agricultural Engineers
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• v.16 no.2
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• pp.3430-3437
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• 1974

.It is very importaut to know the water consumption of crops in planning irrigation works and practicing suitable soil moisture management. For the purpose of making it clear that how much water be consumed to cultivate the Chinese cabbage, Chamber method has been applied. Main equipments in the transpiration chamber are flowers, manometer and electric thermograph. The chamber made of vynyl plate has a small entrance at the base and an exit at the top, and the ventilation in the chamber was carried out by a flower through the entrance and exit. Air-flow adjusted by an orifice manometer enters the chamber from the outside over the crop canopy through the pipe like a chimney and finally goes out to the outside. Two sets which consist of a pair of dry and wet bulb made by thermistor are installed in the entrance and exit tube, and record air temperature automatically. Evapotranspiration amount is computed from the air-flow quantity and difference in absolute humidity between at the entrance and exit of the chamber by the following equation: ET=(X2-X1)${\times}$Q where ET=evapotranspiration amount X1=absolute humidity at the entrance(g/㎥) X2=absolute humidity at the exit(g/㎥) Q=air-flow quantity(㎥) This study was carried out at the upland farm of the Institute of Agriculture Engimeering and Utilization, Suwon, Korea. from 1971 to 1973. The results obtained in this experiment are as follows: 1. The total amount of evapotranspiration of Chinese Cabbage that is cultivated in autumn is 408.1mm during growth period. 2. Chinese cabbage rapidly grows up in the second ten days of September, 40th to 50th days after seeding. At the same time, the maximum amount of evaportranspiration of Chinese cabbage is 61.6mm/10 days 3. The correlation between Pan-evaporation and evapotranspiration is high, coefficient of correlation r=0.88**, and can be shown as The following regression equation: ET=0.913E+20.273 4. Evapotranspiration is closely related with meteorological factors: r=0.85**, for insolation, r=0.76** for air temperature, respectively. 5. The percentage of evapotranspiration amount, at the beginning of growth stage, gradually increases in proportion as the Chinese Cabbage grows but is largely affected by meteorological factors after the green cover formation. 6. By Blaney and Griddle formula, evaportranspiration coefficient "K" are within from 0,85 to 1.27.

• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.223-227
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• 2013

Purpose: The objectives of this study were to develop a general purpose baler system that is appropriate for the domestic forage cultivation environment and operated by the medium size tractor for production of bale silage made of green forage crops, and to test its performance. Methods: In a first experiment, the time of formation per one bale and densities of bales that are produced from bale system, were measured. In a second experiment, power requirement was measured by a power measurement system manufactured during bale system work. Results: The power measurement system was constructed with strain-gage sensors to measure torque of a PTO axle and proximity sensor to measure rotating speed of a PTO axle. Thus, the power requirement was calculated by PTO torque and PTO rotating speed. For evaluation of bale quality, the samples of bales were analyzed for contents of moisture, ADF, NDF and TDN. Conclusions: If the results of this study will be utilized, the coefficient of utilization of agricultural machinery will be increased by the operation of a medium size tractor that is a major disseminated tractor in farm, and it will contribute tremendously to make a forage production base for livestock farms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47
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• pp.150-162
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• 2002

Perilla, Perilla frutescens. (L.) Britton, is a traditional oil seed crops grown in Korea. The seeds and seed oil is used for edible and some industrial sectors. The seeds of perilla contains 35-54% of a drying oil which is similar to the linseed oil. The fatty acids of seed oil is composed with linolenic acid, linoleic acid, and oleic acid. The majority of fatty acids of the oil is $\alpha$-linolenic acid proportioned 51-71% of the oil. This high linolenic acid makes it unstable of the oil and owing to the fast oxidation. Therefore, the plant breeders are challenges to develope a new varieties with low linolenic acid for edlible oil and high linolenic acid for industrial uses. Perilla foliage is also used as a potherb. The green leaves contains a special flavor, perilla aldehyde, and some abundant minerals and vitamins. The vitamin C and $\beta$-carotene is more available than lettuce and crown-daisy of which used for similar potherb and vegetables in traditional Korean food table. The authors are reviewed and discussed on the current status and prospects of the quality evaluations and researches in perilla seeds and leaves to provide and refers the condensed informations on their quality.

• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.668-676
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• 2015

The objective of this paper is to elicit some policy implications to promote agroforestry in South Korea from the consumers' perspective. We conducted internet survey to 640 urban households about the recognition of forest farming products. Consumers purchased forest farming products for their nutrition, medical function, and safety and regarded them as higher quality than crops cultivated from agricultural land. Consumers preferred forest farming products but revealed no difference in recognition of naturally-grown products. Consumers buying forest products prioritize the quality and medical function among attributes. Among consumers as their income increases, they regard naturally grown products more important than price and freshness. Therefore, to promote agroforestry in South Korea, certification for naturally-grown products to win the consumers' trust and the $6^{th}$ industrialization by combining experience and green tourism program must be considered.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.5
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• pp.383-390
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• 2017

Heavy metal contamination of soil might be a cause of serious concern due to the potential health impacts of consuming contaminated products. In this study, the total content of heavy metals (Cd, Cr, Cu, Ni, Pb, Zn, As, and Hg) in soils was analyzed, and the difference of heavy metal contents depending on crops, soil characteristics, and topography was compared in 169 greenhouse soils obtained from Gyeongnam Province. The concentrations of the heavy metals were $0.25mg\;kg^{-1}$ (ranged 0.01~0.44) for Cd, $28.94(0.53{\sim}72.63)mg\;kg^{-1}$ for Cr, $26.03(0.5{\sim}166.13)mg\;kg^{-1}$ for Cu, $14.91(1.27{\sim}33.22)mg\;kg^{-1}$ for Ni, $15.76(0.43{\sim}57.1)mg\;kg^{-1}$ for Pb, $119.72(6.33{\sim}239.39)mg\;kg^{-1}$ for Zn, $2.54(0.01{\sim}23.57)mg\;kg^{-1}$ for As, and $0.049(0.012{\sim}0.253)mg\;kg^{-1}$ for Hg in topsoils. The concentrations of Pb and As in topsoil were highest in green pepper and those of Cd, Cr, and Ni were highest in melon. In addition, the concentrations of Cr and Ni were highest in diluvial terrace compared with the other topographies. Higher concentrations of Cd, Cr, and Ni were found in silty clay loam and silt loam soils than sandy loam and loam soils.

• Journal of Korean Society on Water Environment
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• v.20 no.3
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• pp.301-306
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• 2004

The present water quality standards for agricultural were established without considering the effects of water quality on the safety, growth, yield and quality of crops. This study was carried out to investigate the effects of irrigation water quality on the growth, yield, and grain quality of rice, and to acquire basic knowledges to set up water quality standards for irrigation. The field and pot experiments were conducted with irrigation water that was previously adjusted four concentrations (control, 5, 10, 20 mg/L) and six concentrations (control, 5, 10, 15, 20, 30 mg/L) by $NH_4NO_3$ solution and replicated three and four times with randomized block design, respectively. The results of this study showed that the inorganic nutrient of rice plant, rice protein contents and number of panicle tended to increase as the T-N concentration in irrigation water was increased. In addition, grain yield at T-N 10 mg/L and 20mg/L were significantly higher than the control at the field experiment. From the pot experiment at T-N 30 mg/L, the percentage of head rice was slightly lower due to the increase of green kernel and white belly/core kernel.