• Journal of the Korean Society of Environmental Restoration Technology
• /
• 제23권6호
• /
• pp.57-73
• /
• 2020

A study was conducted to present the criteria for evaluating the revegetation method of the slope of the expressway. The results of comparative analysis between 2019-2020 field survey and existing research data (2015-2016) are as follows. Soil is a very important factor at the beginning of revegetation work. However, after the plant has settled, the importance of the soil composition diminishes. Among the plants used, the number of plants sown at the beginning gradually decreases. And it changes as it competes with invading plants. Among the plants used for sowing, it was found to affect the vegetation composition in the order of exotic grass > native herb and wildflower > native tree. Plant coverage is continuously evaluated as an important factor regardless of the time. The vegetation structure on the slope will change continuously over time. New items need to be evaluated in situations where a lot of time has elapsed since the application of revegetation work. It is desirable to use the current evaluation standard only to perform the evaluation within 1 to 2 years. In the long run, it is necessary to establish a new evaluation standard that adjusts the weights of each item.

• Journal of The Korean Society of Grassland and Forage Science
• /
• 제36권4호
• /
• pp.325-332
• /
• 2016

The objective of this study was to determine the growth characteristics of cool ($C_1$) and warm season grasses ($C_2$) in pastures mixed with $C_1$ and $C_2$ suitable for grazing horses and effect of pastures mixed with tall and short grasses on the intake characteristics of horses. $C_1$ used in this study was Kentucky bluegrass, Redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. The short type grass used as $C_2$ was Bermudagrass. This study had the following four treatment groups: 1) Treatment 1 (Bermudagrass + Kentucky bluegrass + Redtop) 2) Treatment 2 (Bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + Redtop) 4) Treatment 4 (tall fescue + orchardgrass). There was no winterkilling or lodging problem at an altitude of 200 m. Plant heights in mixed pasture of Treatment 1, 2, 3, and 4 were 53.9, 58.2, 57.5, and 78.1 cm, respectively. Plant height was the highest in Treatment 4. Dry matter yield was in the following order : Treatment 4 > Treatment 3 > Treatment 2 > Treatment 1. In the first investigation regarding vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 70% and 66.7%, respectively. Overall, other grasses showed poor growth. In the second investigation of vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 80% and 60.7%, respectively. Crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility value, and nutritive values were the lowest in Treatment 4, followed by those in Treatment 1, Treatment 2 and Treatment 3.

• Weed & Turfgrass Science
• /
• 제7권2호
• /
• pp.140-147
• /
• 2018

In Korea, mowing the grass of a grave (Beolcho) is a very important event preceded by Chuseok (Korean thanks giving day) and Si-Hyang (annual ancestral feast). Because Beolcho usually takes place one or two weeks before Chuseok, the traffic accidents on roads are getting worse and safety accidents are getting more and more. Erroneous Beolcho methods can cause the soil to be exposed and cause the loss of soil or the low shoot density of graveyard grass or weed occurrence. Therefore, the dispersion of the Beolcho timing and the approprite method can be import issues. The present study assessed the effects of grass mowing timing and height before Chuseok on the growth of Zoysia japonica and Z. matrella. The mowing timing was evaluated at 1 week, 2 weeks, 3 weeks, and 4 weeks before Chuseok, and the mowing height differently according to the two type of grass. In Z. japonica, the faster mowing before Chuseok, the longer the plant height, and the coverage rate and shoot density were higher. In Z. matrella, the faster mowing, the longer the shoot length, but the coverage rate and shoot density did not show any difference or tendency. As compared to Z. japonica, the plant height of Z. matrella was kept short regardless of the time of mowing. In both grasses, the plant height was shortened when mowing was done at low cutting height, but the coverage rate and shoot density were decreased. In addition, it was shown that it is advantageous to maintain the shoot density of graveyard lawn by 40-50 mm or 30-40 mm cutting above the ground for Z. japonica or Z. matrella, respectively.

• Applied Biological Chemistry
• /
• 제47권4호
• /
• pp.414-421
• /
• 2004

3D QSAR studies for protox inhibition activities against root and shoot of the rice plant (Orysa sativa L.) and barnyardgrass (Echinochloa crus-galli) by a series of new 1-(5-methyl-3-phenylisoxazolin-5-yl)methoxy-2-chloro-4-fluorobenzene derivatives were conducted based on the results (Sung, N. D. et al.'s, (2004) J. Korean Soc. Appl. Biol. Chem. 47(3), 351-356) using comparative molecular similarity indices analysis (CoMSIA) methodology. Four CoMSIA models, without hydrogen bond donor field for the protox inhibition activities against root and shoot of the two plants, were derived from the combination of several fields using steric field, hydrophobic field, hydrogen bond acceptor field, LUMO molecular orbital field, dipole moment (DM) and molar refractivity (MR) as additional descriptors. The predictabilities and fitness of CoMSIA models for protox inhibition activities against barnyard-grass were higher than that of rice plant. The statistical results of these models showed the best predictability of the protox inhibition activities against barnyard-grass based on the cross-validated value $r^2\;_{cv}\;(q^2=0.635{\sim}0.924)$, non cross-validated, conventional coefficient $r^2\;_{ncv.}$ value $(r^2=0.928{\sim}0.977)$ and PRESS value $(0.255{\sim}0.273)$. The protox inhibition activities exhibited a strong correlation with the steric $(5.4{\sim}15.7%)$ and hydrophobic $(68.0{\sim}84.3%)$ factors of the molecules. Particularly, the CoMSIA models indicated that the groups of increasing steric bulk at ortho-position on the C-phenyl ring will enhance the protox inhibition activities against barnyard-grass and subsequently increase the selectivity.

• Korean Journal of Soil Science and Fertilizer
• /
• 제18권4호
• /
• pp.392-406
• /
• 1985

The soil management practices before transplanting the ginseng plant were studied with two organic matter sources such as a traditional organic matter (wild grass) and commercial organic fertilizer (byproducts of amino acid fermantation) during the late spring to late autumn. During the soil management practices, the soil received 40kg N/10a from five different combination treatments with above two organic matter sources, a wild grass and a commercial organic fertilizer. After the application of the treatments, the soil were ploughed regularly at the interval of 20 days and the changes of physicochemical properties during the soil management practices were investigated. The next year after soil management practices, ginseng plants were transplanted to each treatment, growth and the content of some organic components of ginseng plant were measured for comparision of the different treatments. 1. The decrease in bulk density observed during the first 40 days of management was considered to be the effect of the improved physical conditions caused by ploughing, The decrease in bulk density observed after 40 days of management was considered to be the effect of organic matter. Similar results were observed in particle density, however porosity increased with time. 2. Soil pH tended to decrease during the first 40 days of management, after which period the pH increased and was stabilized. However, CEC increased with organic matter treatment and the exchangeable $NH^+_4-N$ and $NO^-_3-N$ increased in 20 and 40 days after the management practices, respectively, and after that period it became steady. 3. The decomposition rate of treated organic matter was measured by the incubation test in laboratory conditions. The rate of decomposition was rapid during the first 20 days of management, after which period it showed slight changes. 4. The weight of ginseng root significantly increased in the treatment of 10kg N/10a organic fertilizer and 30kg N/10a wild grass. 5. The saponin content of ginseng root was highest in the 40kg N/10a wild grass treatment. The addition of organic fertilizer at the rate of more than 20kg N/10a caused the decrease in the saponin content.

• Journal of Plant Biotechnology
• /
• 제43권4호
• /
• pp.450-456
• /
• 2016

Rhizoctonia leaf blight (large patch) has become a serious problem in Korean lawn grass, which is extremely hard to treat and develops mostly from the roots of lawn grass to wither it away. Rhizoctonia leaf blight (large patch) is caused by Rhizoctonia solani AG2-2 (IV). To develop zoysia japonica with strong disease tolerance against this pathogenic bacterium, ${\beta}-1,3-glucanase$ was cloned from zoysia japonica, which is one of the PR-Proteins known to play a critical role in plant defense reaction. ${\beta}-1,3-glucanase$ is known to be generated within the cells when plant tissues have a hypersensitive reaction due to virus or bacterium infection and secreted outside the cells to play mainly the function of resistance against pathogenic bacteria in the space between the cells. This study utilized the commonly preserved part in the sequence of corn, wheat, barley, and rice which had been researched for their disease tolerance among the ${\beta}-1,3-glucanase$ monocotyledonous plants. Based on the part, degenerate PCR was performed to find out the sequence and full-length cDNA was cloned. E.coli over-expression was conducted in this study to mass purify target protein and implement in vitro activation measurement and antibacterial test. In addition, to interpret the functions of ZjGlu1 gene, each gene-incorporating plant transformation vectors were produced to make lawn grass transformant. Based on ZjGlu1 protein, antibacterial activity test was conducted on 9 strains. As a result, R. cerealis, F. culmorum, R.solani AG-1 (1B), and T. atroviride were found to have antibacterial activity. The gene-specific expression amount in each organ showed no huge difference in the organs based upon the transformant and against 18s gene expression amount.

• Journal of The Korean Society of Grassland and Forage Science
• /
• 제15권3호
• /
• pp.198-206
• /
• 1995

Baled silage making(BS) of selected forage crops was discussed during 1991-1993, to determine the best cutting time of the plants for BS production, BS yields and silage quality. Seven species of forage crops and pasture grasses(rye, barley, spring oat, Italian ryegrass, orchardgrass, alfalfa and grass-legume pasture mixtures) were harvested at different stage of growth from young plant to physiological maturity, and baled in a self constructed square baling chamber. Each bales, measured 90cm length, 60cm width and 50cm height, were wrapped with 0.05mrn thick polyethylene plastic film, and stored in stack silo. Each bales were weighed between 15-20 kg in dry matter basis. The effects of pre wilting and formic acid addition on the silage quality of young plant materials, which contained high water concentration, was also evaluated during the experiment. Rye plant including of barley and spring oat were evaluated as a good materials for baled silage making. Fodder rye produced high quality BS with a value of silage quality point 84(Flieg's point) when the plant harvested at stage of greatest dry matter accumulation by 12.64 tonha. The best quality BS of barley was obtained at stage of hard dough to yellow stage by 11.9 ton/ha dry matter yield and 81 point silage quality. Italian ryegrass and pasture grasses including of orchardgrass, alfalfa and grass-legume pasture mixtures procuced also high quality bale silage by harvesting at stage of late blooming. However pre witting operation and formic acid addition was required for BS production of grass materials because of high water contents. Water contents of Italian ryegrass and other pasture species ranged 18.9%(Italian) to 20.8%(alfalfa). Silage quality point of Italian BS harvested at late blooing was increased from 72 to 88 by 1/2-one day pre wilting and 0.3% formic acid treatment. Silage quality of young plant materials of rye and other forage crops, barley and spring oat were also improved markedly through the pre wilting treatment and formic acid addition.

• Journal of the Korean Institute of Landscape Architecture
• /
• 제30권1호
• /
• pp.61-74
• /
• 2002

The purpose of this study is to propose vegetation management devices through analyzing the actual vegetation, flora, plant community structure and soil chemical concentrations in Weolmi urban nature park, Incheon. The actual vegetation of Weolmi Park in various areas is composed of urbanization area(2 types, 25.9%), landscape planting area(4 types, 16.1%), grass and marsh area(5 types, 7.6%) and mountain forest areal(14 types, 50.4%). The flora is composed of 295 taxa with 80 families, 253 species, 35 varieties and 7 formas, and among them there are 16 naturalized plant families, 39 species, 3 varieties. In reflection of size, the number of the species seems high but most of the them are under influence of human disturbance. Nine survey plots of plant community structure are classified into two groups. One is the semi-natural plant community(Prunus sargentii-Acer palmatum, Quercus accutissma-Prunus sargentii, Quercus serrata-Quercus accutissma-Prunus sargentii, Prunus sargentii, and Zelkova serrata-Prunus sargentii) that migrated finn the planting forest to the natural forest and the other is planting forest(Pinus koraienssis-Pinus thunbergii-Abies holophylla-Chamaectparis obtusa, Prunus sargentii, Pinus thunbergii-Alnus firma, Zelkova serrata). The average pH is 4.65 which means the soil acidity is quite high. The concentration of K, Ca, Mg and base saturation is very low. It seems that the environmental pollutants from Incheon Port and industrial plants near by survey site and long-distance transport of air pollutants from China made the soil condition worse. On the basis of the results above, six vegetation management devices are suggested: 1) removing the hazard plants(Pueraia thunbergiana and Humulus japonica), 2) natural landscape management of the middle and long term, 3) increasing species diversity, 4) Robinia pseudoacacia management, 5) keeping the naturalized plants from being distributed any further inside the mountain forest, 6) improving soil acidification.

• The Plant Pathology Journal
• /
• 제15권1호
• /
• pp.14-20
• /
• 1999

Twenty isolates of Helminthosporium species were obtained from various grass plants and tested for controlling efficacy on the development of plant diseases. An isolate of Helminthosporium sp. TP-4 was chosen and six antibiotic substances were purified from cultures of the fungus by repeated silica gel column chromatography and preparative thin-layer chromatography. They were identified as ophiobolin a, 6-epiophiobolin A, 3-anhydroophiobolin A, 3-anhydro-6-epiophiobolin A, iphiobolin B, and iphiobolin I mainly by mass spectrometry and nuclear magnetic resonance spectrometry. Ophiobolins inhibited the growth of a grampositive bacterium Streptomyces griseus, but were not active against gram-negative bacteria. They also showed an antifungal activity. In in vivo tests, iphiobolin B exhibited potent controlling activities against rice blast, tomato late blight, and wheat leaf rust with control values more than 90% and 70% at concentration of $500\mu\textrm{m}$/ml and 100 ${\mu}{\textrm}{m}$/ml. Ophiobolin A and 6-epiophiobolin A controlled the development of wheat leaf rust more than 80% at concentrations of 100 /ml and $500\mu\textrm{m}$/ml respectively. 3-Anhydro-6-epiophiobolin A was not active against any plant disease. On the other hand, the A-series ophiobolins other than 3-anhydroophiobolin A showed stronger phytotoxic activity in a leaf-wounding assay using 8 plant species than those of 3-anhydroophiobolin A, ophiobolin B, and ophiobolin I. The results indicate that there is little correlation between antifungal activity and phytotoxicity of ophiobolins.

• Journal of Plant Biotechnology
• /
• 제30권2호
• /
• pp.115-121
• /
• 2003

In this study, plant regeneration and Agrobacterium tumefaciens-mediated transformation Kentucky bluegrass(Poa pratensis L.) were evaluated. Three different types of calli were produced depending on the combinations of growth regulators. They were non-friable brown or gray-colored callus (type I), compact, friable and yellow or white-colored callus (typeII), and soft, watery translucent callus with differentiated structure (typeIII). The highest regenerable organogenic callus (typeII) was obtained on the medium containing 1mg/L, 2,4-D and 0.1mg/L BA. Additionally, the production of typeII calli increased significantly when AgNO$_3$ was added to the callus induction and growth medium. The highest frequency of multiple shout formation from typeII callus was obtained on MS medium containing 1mg/L BA and 1mg/L Thidiazuron(TDZ). The organogenic calli(typeII) were inoculated with Agrobacterium tumefaciens strain EHA101 harboring the binary vector pIG121Hm with $\beta$-glucuronidase gene, and various factors were found to influence the transfer-DNA delivery efficiency. The highest transient GUS activity was observed on typeIIcallus. In the present work, we reported the first transient GUS activity of Kentucky bluegrass mediated by Agrobacterium tumefaciens. Our system may contribute to genetic improvement for breed-recalcirtrant grass species, Kentucky bluegrass.