The Plant Pathology Journal
- Volume 15 Issue 1
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- Pages.14-20
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- 1999
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- 2093-9280(eISSN)
Antibiotic and Phytotoxic Activities of Ophiobolins from Helminthosporium Species
- Kim, Hyun-Ju (Antibiotic and Phytotoxic Activities of Ophiobolins from Helminthosporium Species) ;
- Kim, Jin-Cheol (Antibiotic and Phytotoxic Activities of Ophiobolins from Helminthosporium Species) ;
- Kim, Byung-Sup (Antibiotic and Phytotoxic Activities of Ophiobolins from Helminthosporium Species) ;
- Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University, Taejon) ;
- Cho, Kwang-Yun (Antibiotic and Phytotoxic Activities of Ophiobolins from Helminthosporium Species)
- Published : 1999.02.01
Abstract
Twenty isolates of Helminthosporium species were obtained from various grass plants and tested for controlling efficacy on the development of plant diseases. An isolate of Helminthosporium sp. TP-4 was chosen and six antibiotic substances were purified from cultures of the fungus by repeated silica gel column chromatography and preparative thin-layer chromatography. They were identified as ophiobolin a, 6-epiophiobolin A, 3-anhydroophiobolin A, 3-anhydro-6-epiophiobolin A, iphiobolin B, and iphiobolin I mainly by mass spectrometry and nuclear magnetic resonance spectrometry. Ophiobolins inhibited the growth of a grampositive bacterium Streptomyces griseus, but were not active against gram-negative bacteria. They also showed an antifungal activity. In in vivo tests, iphiobolin B exhibited potent controlling activities against rice blast, tomato late blight, and wheat leaf rust with control values more than 90% and 70% at concentration of