• The Plant Pathology Journal
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• v.32 no.4
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• pp.371-376
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• 2016

Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.

• Korean journal of applied entomology
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• v.41 no.2
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• pp.137-143
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• 2002

Grape phylloxera, Daktulosphaira vitifoliae(Fitch), is a destructive grape insect pest because it forms root galls and kills the plant by directly destroying root system. The effects of insecticide treatments were examined to establish the chemical control system of phylloxera, and the damage by this insect was investigated in grapevine yards. The treatments of 40kg carbofuran 3GR per ha and carbosulfan 20WP of 10$\ell$ (1,000$\times$) per tree successfully reduced phylloxera densities. After the insecticide treatments in early season, the phylloxera population rebuilt up again at harvest season. In a grapevine yard where two times of carbofuran treatment (mid-May and post-harvest treatment) were applied a year from 1998 to 2000, total yield per ha in 2000 showed an increase of 30% over that of 1998. But the yield in 2000 was low 20% compared to the average standard yield of Korean grapevine yards. In a grapevine yard without chemical treatment during the same years, total yield per ha in 2000 showed 53% reduction compared to that of 1998. Also, damaged vines by phylloxera abruptly increased from 3% in 1998 to 50% in 2000.

• Research in Plant Disease
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• v.15 no.2
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• pp.77-82
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• 2009

The roots of grapevine in the field in which the crown gall was occurred severely in Chungbuk province were collected and Agrobacterium spp. were isolated from the roots using the selective media. The selected 13 isolates were identified as A. tumefaciens with fatty acid analysis using MIDI system, nucleotide sequence of 16S rDNA, biochemical characteristics, and PCR with the species-specific primers. A. vitis, a pathogen of crown gall disease of grapevine was not isolated from the roots. All of the isolates did not show pathogenicity on the tomato seedlings and the stem and root of grapevine. Eric-PCR showed that DNA band patterns of the root isolates were a little more similar to A. tumefaciens than A. vitis. However, overall similarity between the root isolates and the pathogenic strains of A. tumefaciens and A. vitis was low by rep-PCR. These results suggest that a pathogen causing crown gall in grapevine in Chungbuk province may transmitted through the seedlings rather than via soil or roots.

• Journal of the Ergonomics Society of Korea
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• v.30 no.3
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• pp.395-401
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• 2011

Objective: This study examined the effects of working chair that was developed for farmers who work in grapevine's cluster thinning. Background: Agricultural work involves some of the nation's highest occupational risk exposures. Fruit cultivation has been recognized as one of the most hazardous crops in which to work. Grapevine cluster thinning task involve activities related to the main risk factors associated with upper limb work-related musculoskeletal disorders. Method: An experiment was designed to test the working chair. Six healthy male($25.8{\pm}4.9years$) were selected as the subjects for this study. Electromyography(EMG) was used to monitor the muscles activity, and Electro-goniometer was used to measure the ranges of motions of the elbow, trunk and knee. Subjective test was also carried out to examine discomfort body parts and their pain intensity. A grapevine's working space was built for the experiment and working chair was installed on it. In order to examine the muscle activity and range of motion, subjects used to the working chair for 30 minutes for each experimental condition. Another test without working chair was also performed for comparison. %MVC was used to quantify the level of muscle activity. Results: Decreases of muscle activity was found in all leg muscles and significant decrease of muscle activity was found in left Gastrocnemius. The range of motion of the trunk and knee also decreased when working chair used. Discomfort in lower back, thigh and shank region were reduced significantly. However, in upper limbs muscle activity tended to increase in working chair compared with conventional task. Conclusion: Improvement for cushion in seat back and pan required to reduce discomfort in buttocks. Application: Overall findings verified that the working chair might help to prevent upper limb and lower back MSDs based on the current study. These results can be practically used for work improvement for the grapevine growers to prevent MSDs.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.374-381
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• 2011

Stilbenes are polyphenolic natural products, which have antioxidative and antifungal activities. In some plants, including grapevine, the stilbene compounds, as resveratrol derivatives, exist in very diverse forms. Experiments to identify the individual stilbene compounds were carried out first to quantify them in UV-irradiated grapevine leaves. For this, stilbene glycosides were extracted from grapevine leaves which irradiated intensively with UV light. The glycoside samples were hydrolyzed by ${\beta}$-glucosidase, before analyzed by HPLC-mass spectrometer at each m/z corresponding to the mass of specific stilbenes. As results, in chromatograms, the enzymatic hydrolysis resulted in decrease and increase of the peaks expected for glycosides and aglycones, respectively. The samples were also exposed to sunlight in order to photo-isomerize the stilbene compounds. The light exposure resulted in disappearance and appearance of peaks expected for trans- and cis-isomers of stilbenes, respectively. Such a change of the peaks in chromatograms provided information needed for the inference to peak components. In this way, it was possible to identify 16 kinds of stilbene compounds from grapevine leaves. The identified stilbenes were quantified from grapevine leaves irradiated mildly by UV light. The UV-irradiation increased markedly in the content of stilbene compounds, especially trans-resveratrol by several hundredfold. In addition, piceatannol, which is a mere minor component of stilbenes in control leaves and a more active radical scavenger than resveratrol, was also increased by several tenfold by the treatment. The increase in stilbene contents as influenced by UV irradiation seems to be one of the stress coping responses of grapevine as a hormesis phenomenon.

• The Korean Journal of Pesticide Science
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• v.4 no.1
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• pp.38-43
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• 2000

As the first step of a research on the establishment of control strategy for grapevine diseases and insects, the current status of pest control and yield losses by them were surveyed from grapevine growers of Korea. For insects, the most difficult to control was a grapevine stem borer and a grapevine clearwing moth was the next. On the other hand, several diseases including anthracnose, downy mildew, powdery mildew, bird's eye rot, leaf blight, were answered by the growers. The occurrence of the pests varied to the cultural practices. Yield loss was more serious in rainfall intercept culture than in plastic film house culture and diseases caused more loss in yield than insects did. However, the grapevine grower's potential for the identification of the kinds of pests was not high enough. When the pest was observed, the growers consulted mainly with local pesticide dealers or neighbouring growers and usually applied the pesticides prescribed by the dealer. More than half of the growers did not use pest control calendar, and most of the growers applied pesticides before any symptom appeared. Also, more than half of the growers applied mixture of at least more than 1 kind of pesticide and nutrients. Insecticides were applied less than 5 times during the season, but it was more than 6 times for fungicides. In the pesticide selection, the growers checked control effect first, regardless of the registration, and the pest control cost per unit area varied very much depending on the growers.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.327-335
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• 2012

Nutrient uptake of each part of grapevine in the new grapevine cultivars (Jinok, Hongisul) was analyzed for making standard of annual fertilizations at four years. One year grown diploid cultivar 'Jinok' was showed more vigorous growth of root than other cultivar. Annually total nutrient of grapevine was absorbed with the same ratio of three major nutrients (Nitrogen, Phosphate, and Potassium). However about 30% of total absorbed nutrient of 3~4 year grown grapevine was distributed bunches. Potassium was measured as major nutrient in the bunch, accumulated in the peel and flesh (about 1% of D.W.). Magnesium was mainly accumulated in the petiole (about 1% of D.W.). Calcium has accumulated in the leaf (about 0.95% of D.W.), that concentration in this part was similar concentration of nitrogen (about 1.25% of D.W.). Nutrient requirement of four year grown 'Jinok' (N; 55.5 g, P; 7.7 g, K; 42.0 g, Ca; 34.6 g, Mg; 11.1 g) required less fertilizers than 'Campbell Early' (N; 57.4 g, P; 7.9 g, K; 44.4 g, Ca; 37.3 g, Mg; 12.2 g) needed. 'Hongisul' required less fertilizers compared to other grapevine cultivars, but cultural practice system for production of grape should be develop to improve their poor bud burst and fruit set.

• Biomolecules & Therapeutics
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• v.9 no.3
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• pp.188-193
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• 2001

Free radical scavengers or quenching agents for reactive oxygen species (ROS) present in consumable fruits, vegetables, and beverages have received considerable attention as potential antioxidants, and thus uses for treatment of several human diseases. In this study, grapevine shoot extract (GSE) containing high concentration of resveratrol and viniferine was evaluated for antioxidant potential and inhibition of pro-inflam-matory mediator production. Utilizing 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and xanthine oxidase (XOD) inhibition assay the GSE showed inhibitory effects of DPPH radical scavenging and XOD activity with the $IC_{50}$/ values of 34.5 and 155 $\mu\textrm{g}$/ml, respectively. In addition, GSE also exhibited the inhibition of prostaglandin E$_2$ (PGE$_2$) and nitric oxide (NO) production in lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 cells with the $IC_{50}$/ value of 6.4 and 14.5 $\mu\textrm{g}$/ml, respectively. This result suggests that grapevine shoot extract has the potential activity as a natural antioxidant or antiinflammatory agent.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.155-160
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• 2003

Grapevine leafroll-associated virus 3(GLRaV-3) is one of the most severe pathogens for viral diseases found in Korea. This study was conducted to establish the virus-free stock production system for the virus disease control. The effects of thermotherapy, merestem culture and chemotheratpy to eliminate the GLRaV-3 in gratevines were tested. Thermotherapy at 37$\pm$2$^{\circ}C$ for 6∼8 weeks combined with 0.5∼1.0mm size of meristem culture method was the most effective for virus elimination. Thermotherapy alone was not effective. In chemotheratpy, DHT and Amantadine (20, 40mg/L) treatment in medium was more effective than Ribavirin to eliminate the GLRaV-3 in grapevine. However, Ribavirin spraying to potted was not available for virus elimination. Therefore, virus-free stock production system using the thermotherapy combined with shoot apical meristem culture was the most effective in grapevine.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.335-339
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• 2001

Grapevine leafroll-associated 3 closterovirus (GLRaV-3) is phloem limited virus, and one of the most severe viral diseases found in Korea. However, nonhomogenous distribution and low concentration and seasonal variations of GLRaV-3 in grapevines remain as main problems which prevent the introduction and molecular biology or serology experiments. Virus-infected plantlet in vitro was obtained from node tissue cuttings, which was GLRaV-3 infected 'kyoho' vines. The amount of purified virus was highest in vitro plantlet. Moreover, viruses seem to be relatively homogeneously distributed in all organs including leaf, stem and callus derived from in vitro plantlets. RT-PCR detection using in vitro plantlet tissue as template was most effective. When comparing ELISA to RT-PCR, RT-PCR detection was 1,000 times as effective as ELISA. These results can be explained by improved quality such as tenderness or less tannins in plantlet in vitro. In conclusion, until infected herbaceous host will be available, tissue culture can be usefully adopted as a technique for a good source of GLRaV-3 closterovirus for further studies.