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http://dx.doi.org/10.5423/PPJ.NT.11.2015.0237

Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus  

Park, Sang-Ho (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Choi, Hoseong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Kim, Semin (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Cho, Won Kyong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Kim, Kook-Hyung (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Publication Information
The Plant Pathology Journal / v.32, no.4, 2016 , pp. 371-376 More about this Journal
Abstract
Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.
Keywords
Grapevine Algerian latent virus; virus-based;
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1 Lovato, A., Faoro, F., Gambino, G., Maffi, D., Bracale., M., Polverari, A. and Santi, L. 2014. Construction of a synthetic infectious cDNA clone of grapevine Algerian latent virus (GALV-Nf) and its biological activity in Nicotiana benthamiana and grapevine plants. Virol. J. 11:186.   DOI
2 Muruganantham, M., Moskovitz, Y., Haviv, S., Horesh, T., Fenigstein, A., du Preez, J., Stephan, D., Burger, J. T. and Mawassi, M. 2009. Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera. J. Virol. Methods 155:167-174.   DOI
3 Myles, S., Boyko, A. R., Owens, C. L., Brown, P. J., Grassi, F., Aradhya, M. K., Prins, B., Reynolds, A., Chia, J. M., Ware, D., Bustamante, C. D. and Buckler, E. S. 2011. Genetic structure and domestication history of the grape. Proc. Natl. Acad. Sci. U. S. A. 108:3530-3535.   DOI
4 Ohki, T., Uematsu, S., Nakayama, Y., Lesemann, D. E., Honda, Y., Tsuda, S. and Fujisawa, I. 2006. Characterization of grapevine Algerian latent virus isolated from nipplefruit (Solanum mammosum) in Japan. J. Gen. Plant Pathol. 72:119-122.   DOI
5 Pignatta, D., Kumar, P., Turina, M., Dandekar, A. and Falk, B. W. 2007. Quantitative analysis of efficient endogenous gene silencing in Nicotiana benthamiana plants using tomato bushy stunt virus vectors that retain the capsid protein gene. Mol. Plant-Microbe Interact. 20:609-618.   DOI
6 Pogue, G. P., Lindbo, J. A., Garger, S. J. and Fitzmaurice, W. P. 2002. Making an ally from an enemy: plant virology and the new agriculture. Annu. Rev. Phytopathol. 40:45-74.   DOI
7 Powers, J. G., Sit, T. L., Heinsohn, C., George, C. G., Kim, K. H. and Lommel, S. A. 2008. The red clover necrotic mosaic virus RNA-2 encoded movement protein is a second suppressor of RNA silencing. Virology 381:277-286.   DOI
8 Scholthof, H. B., Scholthof, K. B. and Jackson, A. O. 1995. Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell 7:1157-1172.   DOI
9 Scholthof, K. B., Mirkov, T. E,. and Scholthof, H. B. 2002. Plant virus gene vectors: biotechnology applications in agriculture and medicine. Genet. Eng. (N. Y.) 24:67-85.
10 Seaberg, B. L., Hsieh, Y. C., Scholthof, K. B. and Scholthof, H. B. 2012. Host impact on the stability of a plant virus gene vector as measured by a new fluorescent local lesion passaging assay. J. Virol. Methods 179:289-294.   DOI
11 Shamekova, M., Mendoza, M. R., Hsieh, Y. C., Lindbo, J., Omarov, R. T. and Scholthof, H. B. 2014. Tombusvirusbased vector systems to permit over-expression of genes or that serve as sensors of antiviral RNA silencing in plants. Virology 452-453:159-165.   DOI
12 Yi, L., Lesemann, D. E., Koenig, R., Rudel, M. and Pfeilstetter, E. 1992. Isometric plant viruses in ditches and streams in agricultural areas: recovery of previously found viruses and identification of hitherto unrecorded carmoand tombusviruses including grapevine Algerian latent virus. J. Phytopathol. 134:121-132.   DOI
13 Brigneti, G., Martin-Hernandez, A. M., Jin, H., Chen, J., Baulcombe, D. C., Baker, B. and Jones, J. D. 2004. Virus-induced gene silencing in Solanum species. Plant J. 39:264-272.   DOI
14 Burch-Smith, T. M., Anderson, J. C., Martin, G. B. and Dinesh-Kumar, S. P. 2004. Applications and advantages of virusinduced gene silencing for gene function studies in plants. Plant J. 39:734-746.   DOI
15 Cannizzaro, G., Rosciglione, B. and Castellano, M. A. 1990. The presence of phytopathogenic viruses in waterways of western Sicily. Inf. Fitopatol. 40:55-56.
16 Dalmay, T., Rubino, L., Burgyan, J., Kollar, A. and Russo, M. 1993. Functional analysis of cymbidium ringspot virus genome. Virology 194:697-704.   DOI
17 Dawson, W. O. and Folimonova, S. Y. 2013. Virus-based transient expression vectors for woody crops: a new frontier for vector design and use. Annu. Rev. Phytopathol. 51:321-337.   DOI
18 Fofana, I. B., Sangare, A., Collier, R., Taylor, C. and Fauquet, C. M. 2004. A geminivirus-induced gene silencing system for gene function validation in cassava. Plant Mol. Biol. 56:613-624.   DOI
19 Fuchs, E., Gruntzig, M., Auerbach, I., Einecke, I., Muller, C. and Kragenow, M. 1994. On the occurrence of plant pathogenic viruses in waters in the region of Halle/Saale (German Federal State of Saxony-Anhalt). Arch. Phytopathol. Plant Prot. 29:133-141.   DOI
20 Fujinaga, M., Ogiso, H., Wakabayashi, H., Morikawa, T. and Natsuaki, T. 2009. First report of a grapevine Algerian latent virus disease on statice plants (Limonium sinuatum) in Japan. J. Gen. Plant Pathol. 75:157-159.   DOI
21 Gallitelli, D., Martelli, G. P. and Di Franco, A. 1989. Grapevine Algerian latent virus, a newly recognized tombusvirus. Phytoparasitica 17:61-62.
22 Gleba, Y., Klimyuk, V. and Marillonnet, S. 2007. Viral vectors for the expression of proteins in plants. Curr. Opin. Biotechnol. 18:134-141.   DOI
23 Haviv, S., Galiakparov, N., Goszczynski, D. E., Batuman, O., Czosnek, H. and Mawassi, M. 2006. Engineering the genome of grapevine virus A into a vector for expression of proteins in herbaceous plants. J. Virol. Methods 132:227-231.   DOI
24 Hefferon, K. L. 2012. Plant virus expression vectors set the stage as production platforms for biopharmaceutical proteins. Virology 433:1-6.   DOI
25 Holzberg, S., Brosio, P., Gross, C. and Pogue, G. P. 2002. Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J. 30:315-327.   DOI
26 Kang, M., Seo, J. K., Cho, H., Choi, H. S. and Kim, K. H. 2016. Establishment of a simple and rapid gene delivery system for cucurbits by using engineered zucchini yellow mosaic virus. Plant Pathol. J. 32:70-76.   DOI
27 Kim, S., Cho, W. K., Lee, H. G., Park, S. H., Sohn, S. H. and Kim, K. H. 2012. The p19 protein of grapevine Algerian latent virus is a determinant of systemic infection of Chenopodium quinoa. Virus Res. 165:81-89.   DOI
28 Koenig, R., Verhoeven, J. T., Fribourg, C. E., Pfeilstetter, E. and Lesemann, D. E. 2004. Evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates. Arch. Virol. 149:1733-1744.
29 Kumagai, M. H., Donson J., della-Cioppa, G., Harvey, D., Hanley, K. and Grill, L. K. 1995. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc. Natl. Acad. Sci. U. S. A. 92:1679-1683.   DOI
30 Kurth, E. G., Peremyslov, V. V., Prokhnevsky, A. I., Kasschau, K. D., Miller, M., Carrington, J. C. and Dolja, V. V. 2012. Virusderived gene expression and RNA interference vector for grapevine. J. Virol. 86:6002-6009.   DOI
31 Liu, Y., Schiff, M. and Dinesh-Kumar, S. P. 2002. Virus-induced gene silencing in tomato. Plant J. 31:777-786.   DOI