• Nutrition Research and Practice
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• 제4권2호
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• pp.114-120
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• 2010

The effects of grape seeds extract and grape peels extract prepared from grape pomace on the activity of antioxidant enzymes, degree of lipid peroxidation in serum and liver tissue were investigated in rabbits fed on high cholesterol diet. New Zealand white rabbits were divided as follows ; 1) NOR (normal group); 2) CHOL (cholesterol group); 3) GSH (cholesterol + grape seed extract group); 4) GPE (cholesterol + grape peel extract); 5) GSP (cholesterol + grape seed powder); 6) GPP (cholesterol + grape peel powder); 7) GE (cholesterol + grape seed and peel extract); 8) GP (cholesterol + grape seed and peel powder). Eight groups of rabbits were studied for 8 weeks. At the end of the experimental period, rabbits were sacrificed and the liver tissue were removed. Then, GSH, GPx, GST, CAT and MDA in the liver were measured. In liver tissues, total glutathione contents (GSH), glutathione peroxidase (GPx) and catalase (CAT) activity, which was significantly higher by grape seed extract supplementation. The level of malondialdehyde (MDA) was lower in the serum of rabbits fed grape seed extract or grape peel powder plus cholesterol than in the serum of rabbits fed cholesterol alone. It is therefore likely that grape seed extract prepared from grape pomace functioned as antioxidants in vivo, negating the effects of the oxidative stress induced by 1% cholesterol diet. The grape seed extract was found effective in converting the oxidized glutathione into reduced glutathione, and in removing $H_2O_2$ that is created by oxidative stress. The grape peel powder was found to have small influence on reduced glutathione content, CAT and GPX activity, but it increased GST activity in liver tissues, resulting in promoting the combination of lipid peroxide and glutathione (GSH), and further, lowering the formation of lipid peroxide in the serum. Therefore, grape pomace (grape seed extract and grape peel powder) supplementation is considered to activate the antioxidant enzyme system and prevent damage with hypercholesterolemia.

• 한국응용과학기술학회지
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• 제26권3호
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• pp.341-349
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• 2009

Natural grape extraction was extracted from grape component using diethyl ether as a solvent, and we tested various pharmaceutical and chemical characteristics of this extract. Characteristic experiments to use natural grape extract tested antimicrobial experiment using microbe in pharmaceutical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of characteristics experiment, it obtained about 7.5%-grape extraction ratio as semi-solid state, and after dried in freezing from grape extract of semi-solid state, it obtained about 10%-grape extraction ratio as solid state of dark purple color. From result of antimicrobial experiment of grape extract, number of staphylococcus aureus (KCMC-01) and aspergillus niger (KCMC-02) in microbe decreased more and more according to time passage. This phenomenon showed that grape extract influences to antimicrobial effect. From the result of dye experiment of grape extract, it appeared in direction of dark purple color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope(OEM), we could know that it appears darker silk than cotton.

• 한국식품조리과학회지
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• 제12권3호
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• pp.291-294
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• 1996

This study attempted to examine the effect on sensory characteristics and physiochemical properties of Grape jelly in various volume of adding carrageenan. The pH of Grape extract was 3.98. The viscosity was increased and the turbidity was decreased according to the increase concentration Grape jelly. The sensory evaluation of Grape jelly was the best with the ratio 0.5% carrageenan. The sensory evaluation of Grape jelly mold salad showed that the most preferable jelly was the one made by 0.9% carrageenan and 40% fruits(apple, orange and sweet persimmons).

• 한국식생활문화학회지
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• 제21권3호
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• pp.330-335
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• 2006

The objectives of this study were to characterize the quality of soy kefir made with soymilk in combination with fructose (5%, 10%) and one of the extracts from orange (10%, 15%) and grape (5%, 10%) with differently adjusted amounts as defendant variables. The lactic acid bacteria, yeast and total microbial counts of soy kefir were respectively $1.3{\times}10^7$ CFU/ml, $1.6{\times}10^8$ CFU/ml, $1.5{\times}10^8$ CFU/ml, soy kefir was propered to drink. pH of soy kefir mixed by orange and grape extracts was decreased significantly according to add fructose 5%. Acidity became significantly high when orange and grape extracts were added, which means acidity showed similar tendency in the opposite direction. The saccharinity of soy kefir was not significantly in orange extract, but soy kefir added fructose 10% was high more than fructose 5% in grape extract. In sensory evaluation, soy kefirs added orange extract 15%, fructose 5% and grape extract 10%, fructose 5% were estimated highly on color, astrigent taste, sour taste, mouth feel and overall quality.

• 청정기술
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• 제27권4호
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• pp.306-314
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• 2021

포도껍질은 항산화 물질을 다량 함유한 유용자원임에도 불구하고 사람들은 포도의 과육만 섭취하고 포도껍질을 폐기하고 있다. 본 연구에서는 포도껍질 추출물을 기능성 화장품 원료로의 사용 가능성을 조사하였다. 건조된 포도껍질 분말을 증류수에 넣고 1 h 동안 교반시킨 후 고형물로부터 분리된 상등액을 추출물로 사용하였다. 추출 수율은 17.8 ~ 31.4%였고 추출물에 함유된 총 플라보노이드와 폴리페놀 함량은 각각 1.8 ~ 2.5 mg-QE g-extract^-1와 16.9 ~ 20.3 mg-GAE g-extract^-1였다. 추출물은 2,2-diphenyl-1-picrylhydrazyl (DPPH)와 Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS^TM) 라디칼을 효과적으로 제거하였고 소거정도는 추출물 농도에 따라 증가하였다. Collagenase의 콜라겐 가수분해 활성이 추출물에 의해 저해를 받았고 추출물 농도 증가에 따라 활성 저해율이 84.2%까지 증가하였다. 그러나 추출물에 의한 tyrosinase 활성 저해가 크지 않았다. 추출물에 편백수를 첨가함에 따라 tyrosinase 활성 저해율이 높아졌으나 DPPH 라디칼 소거활성은 오히려 감소하였다. 본 연구를 통하여 포도껍질 추출물은 항산화능과 주름개선 효능이 높은 반면 미백 효능은 낮은 것으로 확인되었다. 추출물에 편백수를 적절히 혼합함으로써 항산화능과 주름개선 효능이 우수할 뿐만 아니라 미백 효능도 향상되었다.

• 한국식품영양과학회지
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• 제32권1호
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• pp.109-114
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• 2003

포도종실 에탄올 추출물의 각 검정 균주에 대한 생육 억제효과를 페이퍼디스크법 및 액체배양법에 의해 조사한 결과, B. subtilis ATCC 6538 혹은 S. aureus. ATCC 6538에 대하여는 농도 의존적인 증식억제 효과를 나타냈으며 P. aeruginosa IFO 3080 및 Sal. enteritidis. IFO3313에 대해서는 제한적인 증식억제 효과를 나타내기도 하였으나 E. coli ATCC 25922 에 대해서는 뚜렷한 항균 효과를 확인하기 어려웠다. 또한 포도종실 에탄을 추출물의 순차 용매 분획물 중에서 에틸아세테이트 분획과 부탄올 분획물만이 각각 35.7 mg/g과 20.2 mg/g의 카테킨을 함유하고 있었는데 에틸아세테이트 분획에 비하여 부탄을 분획물이 각 검정 균주에 대해 뚜렷한 항균활성 을 나타내었다. 부탄올 분획물은 에틸아세테이트 분획물에 비하여 카테킨의 절대 함량은 적었지만 Cl8 카트리지로 소분획하였을 때 고분자체 중심의 폴리페놀 화합물로 구성되어 있음을 알 수 있었다. 따라서 포도종실 에탄올 추출물 의 항균 활성은 카테킨과 같은 플라반-3-올 화합물의 절대 함량과 더불어 플라반-3-올 화합물의 중합도가 관계하는 것으로 예측되었다.

• Food Science and Biotechnology
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• 제14권6호
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• pp.715-721
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• 2005

Antioxidant activities of grape seeds extracted with various solvents were evaluated by measuring total phenol and flavanol contents, thiobarbituric acid reactive substances (TBARS) following lipid peroxidation, 2-deoxyribose degradation, SOD-like activity, 2,2'-azino-bis(3-ethylbenzthizaoline-6-sulfonic acid (ABTS) radical-scavenging ability, and electron-donating ability using 1,1-diphenyl-2-pycryl hydrazil (DPPH) method. Total phenol and flavanol contents of mixted-solvent extracts were higher than those of single-solvent extracts, with the mixing ratio of 17:3 (ethyl acetate: water) (EW) showed the highest contents. Antioxidant activities (%) of TBARS following phosphatidylcholine peroxidation were 14, 45, 45, 7, 4, 25, 21, 23, and 20% for ascorbic acid (AA), butylated hyroxytoluene (BHT), quercetin (Q), acetone extract (AT), ethyl acetate (EA) extract, methanol (MeOH) extract, 4:1 (EA) extract, 9:1 (EW)-extract, and 17:3 EW extract, respectively. Antioxidant activities for 2-deoxyribose degradation were 5, 80, 87, 78, 56, 73, 64, 60, and 75% in AA, BHT, Q, AT, EA, MeOH extract, 4:1 EW extract, 9:1 EW extract, and 17:3 EW extract, respectively. MeOH grape seed extract showed distinctly stronger electron-donating activity than other solvent extracts.

• Food Science and Biotechnology
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• 제16권6호
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• pp.884-888
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• 2007

The aim of this study was to analyze the antioxidant properties of different cultivars of grape skin extract in an in vitro system. The extracts were prepared from eight grape cultivars: 'Campbell Early' (CE), 'Kyoho' (K), 'New Kyoho' (NK), 'Muscat of Alexandria' (MOA), 'Seibel' (S), 'Morgen Schow' (MS), 'Gold Finger' (GF), and 'Meru' (M). The total phenolic acid contents were highest in MS and K. Resveratrol content was high in NK (50.88 mg/l g of coat), and quercetin content was significantly higher in K (0.68 mg/l g of coat) than in the other grape species (0.21-0.44 mg/l g of coat). The K and MS grape species, in which total phenol content was comparatively high (K: $24.15\;{\mu}g/mL$, MS: $25.52\;{\mu}g/mL$), also showed a high level of electron donating activity (K, 53%; MS, 59%). The hydrogen radical scavenging activity of M (50.36%) was significantly higher than the other grape species, including the S (50.21%), MS (49.43%), and K (49.06%) cultivars. Antioxidant activity varied depending on grape species, but overall it was highest in the MS and K cultivars.

• Nutrition Research and Practice
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• 제2권4호
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• pp.227-233
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• 2008

The aim of the present study was to assess the anti-obesity effects of grape seed extract (GSE) supplement in C57BL/6J mice. Thirty mice were divided into three groups; normal diet control group (ND), high fat diet control group (HD) and high fat diet plus grape seed extract supplemented group (HD+GSE). Results were as follows: 1. GSE supplement reduced the weight gain in mice fed high fat diets; epididymal and back fat weights, were lower compared to non-supplemented HD group. 2. Blood lipid concentrations were lower in the HD+GSE group than in the HD group. Serum HDL-C concentrations were higher in the HD+GSE group compared with the other groups. 3. The concentrations of acid-insoluble acylcarnitines, (AIAC) in serum and liver were higher in the HD+GSE group than in the HD group. 4. GSE supplementation increased mRNA levels of lipolytic genes such as carnitine palmitoyltransferase-l (CPT-1) and decreased mRNA levels of lipogenic genes such as acetyl CoA carboxylase (ACC). These findings suggest that grape seed extract supplements in high fat diet might normalize body weight, epididymal and back fat weights, lipid concentrations, and carnitine levels through controlling lipid metabolism.

• KSBB Journal
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• 제24권5호
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• pp.477-482
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• 2009

본 연구는 포도가공부산물로부터 유용성분을 추출하여 자원의 활용과 환경오염을 줄이고, 공정을 간결하게 하여 실제산업현장에서 이용할 수 있는 공정의 개발을 목적으로 하였다. 포도가공 부산물을 포도과피와 포도씨로 나누어 과피에서는 anthocyanin을 씨에서는 oligomeric proanthocyanidin (OPC)를 주 대상물질로 하여 추출하기 위한 공정을 개발하였다. 추출된 항산화물질은 HPLC를 이용하여 정량하였으며, 이들의 효능은 DPPH법, SOD법을 이용하여 대조군들과의 비교를 통하여 확인하였다. 두 방법 모두 포도씨 추출물의 효능이 포도과피 추출물보다 뛰어난 항산화도를 보였다. 이렇게 생산된 추출물을 이용하여 multiple lamella emulsion의 화장품 제형을 개발하였고 이 제형의 안전성과 안정성을 실험적으로 확인하였다.