KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction and Isolation of Antioxidant Fraction from Waste of Grape Products for Cosmetic Application

포도가공 부산물로부터 화장품용 항산화물질의 추출 및 분리

  • Kim, Eui-Jin (Department of Chemical & Biochemical Engineering, Chosun University) ;
  • Lee, Tae-Ho (Department of Chemical & Biochemical Engineering, Chosun University) ;
  • Shin, Hyun-Jae (Department of Chemical & Biochemical Engineering, Chosun University)
  • 김의진 (조선대학교 공과대학 생명화학공학과) ;
  • 이태호 (조선대학교 공과대학 생명화학공학과) ;
  • 신현재 (조선대학교 공과대학 생명화학공학과)
  • Published : 2009.10.29
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Abstract

Anthocyanin and origomeric proanthocyanidin (OPC) fractions showing antioxidizing activity are present in grape extracts. Grape extracts are widely used in cosmetic applications as functional ingredients. The aim of our study is to isolate the antioxidant fraction from waste of grape products. The extraction was done using soxhlet apparatus. Next, the extraction was subjected for identification of antioxidants by using HPLC. Antioxidant assay was performed by using 2,2-diphenyl-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD) method. Antioxidizing activity was found to be higher in grape seed extract when compared to grape skin extract. Using the extract, a novel formula with multi lamella emulsion structure has been developed and its safety and stability were confirmed by standard protocols.

본 연구는 포도가공부산물로부터 유용성분을 추출하여 자원의 활용과 환경오염을 줄이고, 공정을 간결하게 하여 실제산업현장에서 이용할 수 있는 공정의 개발을 목적으로 하였다. 포도가공 부산물을 포도과피와 포도씨로 나누어 과피에서는 anthocyanin을 씨에서는 oligomeric proanthocyanidin (OPC)를 주 대상물질로 하여 추출하기 위한 공정을 개발하였다. 추출된 항산화물질은 HPLC를 이용하여 정량하였으며, 이들의 효능은 DPPH법, SOD법을 이용하여 대조군들과의 비교를 통하여 확인하였다. 두 방법 모두 포도씨 추출물의 효능이 포도과피 추출물보다 뛰어난 항산화도를 보였다. 이렇게 생산된 추출물을 이용하여 multiple lamella emulsion의 화장품 제형을 개발하였고 이 제형의 안전성과 안정성을 실험적으로 확인하였다.

Keywords

References

  1. Shin, H. J., B. S. Kang, J. B. Ahn, and B. H. Kim (2007) Isolation and purification of resveratrol from a grape twig. Korean J. Biotechnol. Bioeng. 22: 351-355
  2. Luz, R., C. Erasmo, M. Julia, M. Carles, R. Joan, G. Xavier, G. Terasa, S. Xavier, and S. Antoni (2009) Recovery of organic wastes in the spanish wine industry. Technical, economic and environmental analyses of the composting process. J. Cleaner Prod. 17: 830-838 https://doi.org/10.1016/j.jclepro.2008.12.005
  3. Kim, H., J. Deshane, S. Barnes, and S. Meleth (2006) Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds. Life Sci. 78: 2060-2065 https://doi.org/10.1016/j.lfs.2005.12.008
  4. Sandra, P., T. L. Maria, G. Giuseppe, A. Donato, and L. N. Ennio (2009) Rapid screening for anthocyanins and anthocyanin dimers in crude grape extract by high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry. J. chromatogr. A 1216: 3864-3868 https://doi.org/10.1016/j.chroma.2009.02.068
  5. Mauro, B., C. Loredana, E. Elisa, L. Giulia, L. Luiga, R. Leonardo, S. Trifone, and V. Giuseppe (2008) An innovative method for purification of anthocyanins from grape skin extracts by liquid and sub-critical carbon dioxide. Sep. Purif. Technol. 64: 192-197 https://doi.org/10.1016/j.seppur.2008.10.012
  6. Choi, Y. and J. Lee (2009) Antioxidant and antiproliferative properties of a tocotrienol-rich fraction. Food Chem. 114: 1386-1390 https://doi.org/10.1016/j.foodchem.2008.11.018
  7. Furiga, C., A. Lonvaud-Funel, and C. Badet (2009) In vitro study of antioxidant capacity and antibacterial activity on oral anaerobes of a grape seed extract. Food Chem. 113: 1037-1040 https://doi.org/10.1016/j.foodchem.2008.08.059
  8. Yang, J., T. E. Martinson, and R. H. Liu (2009) Phytochemical profile and antioxidant activities of wine grapes. Food Chem. 116: 332-339 https://doi.org/10.1016/j.foodchem.2009.02.021