• 한국어병학회지
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• 제16권2호
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• pp.111-118
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• 2003

Androgen plays an important role in the regulation of gonadotropin production in vertebrates . We have investigated the transcriptional pattern of androgen receptor (AR) in a variety of tissues in maturing male and female goldfish by RT-PCR. Specific primer for AR was designed based on goldfish AR gene from the GenBank (accession number AY090897). AR was shown 10 be maturity- and tissue-dependent gene expression pattern in goldfish. In immature male goldfish, significantly higher transcript level of AR was observed in the pituitary und testis , compared [0 brain and liver. Mature male goldfish showed a similar expression pattern to immature male goldfish. Interestingly. when compare to male goldfish, female goldfish showed AR mRNA expression that was found 10 be weak in pituitary, and very low expression in brain. They could not be found 10 have expression in any other tissues. Taken together. the- transcriptional analysis of AR depending on the tissue, sex. and maturity of a goldfish provides the opportunity for the study of goldfish reproductive physiology ,The results provided for the first time a comparison of the tissue distribution of AR mRNA in sexually maturating male and female goldfish.

• 한국수산과학회지
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• 제51권4호
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• pp.383-388
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• 2018

Goldfish hematopoietic necrosis (GHN) affects hematopoietic organs and causes high mortality in goldfish. The causative agent of GHN is cyprinid herpesvirus 2 (CyHV-2). The purpose of this study was to detect CyHV-2 in ornamental cyprinid fish. CyHV-2 monitoring was conducted on a monthly basis for 1 year using goldfish Carassius auratus and pearlscale goldfish Carassius auratus that had been cultured at a domestic aquafarm and imported from Singapore, respectively. The results of polymerase chain reaction (PCR) assays using specific primers for the CyHV-2 helicase gene showed that 27.8% of goldfish and 33.3% of pearlscale goldfish were positive for CyHV-2. No cytophatic effects were detected in koi fin or common carp brain cells inoculated with tissue homogenates from either fish. Our data provides the useful data for establishing future quarantine and disinfection policies.

• 생명과학회지
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• 제22권8호
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• pp.1046-1051
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• 2012

$^{18}F$ 방사성동위원소 피폭으로 금붕어 뇌에서 유도된 생리활성물질을 조사하기 위해 금붕어 뇌에 존재하는 신경전달물질의 생성 변화에 관한 연구를 수행하였다. $^{18}F$ 580 mCi를 납으로 밀폐된 공간에 두고 선원으로부터 4 cm 떨어진 위치에 금붕어들을 수조에 넣어 4시간 동안 노출시켰다. 거리, 노출시간 및 $^{18}F$의 반감기로부터 계산된 흡수선량은 약 2 Gy이었다. $^{18}F$에 의해 피폭된 금붕어 뇌와 피폭되지 않은 정상 금붕어의 뇌를 각각 10마리씩 절개하여 즉시 냉동 보관 하였다. 각각의 조직들은 초산으로 추출하였으며, 동결건조 후 샘플들은 증류수로 녹여 HPLC를 사용하여 물질을 정제하였다. $^{18}F$로 피폭된 금붕어와 피폭되지 않은 금붕어의 뇌에 존재하는 생리활성물질의 흡광도의 세기는 전체적으로 커다란 차이점은 없었지만, 방사선에 노출된 금붕어 뇌 추출물의 경우 13분대에 해당하는 물질 피크만이 정상 금붕어 뇌 추출물에 비해 매우 크게 증가하였다. 분석 결과, 이 물질은 트립토판(tryptophan, Trp)으로 밝혀졌다. 따라서 금붕어의 뇌에 존재하는 신경전달물질인 트립토판은 $^{18}F$ 방사성동위원소 피폭으로 금붕어 뇌에서 농도 변화가 유도된다는 것을 이 결과는 나타내고 있다.

• 한국해양생명과학회지
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• 제1권1호
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• pp.41-49
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• 2016

Kisspeptin (Kiss) and its cognate receptor, kisspeptin receptor (KissR; G protein coupled receptor 54, GPR54), have recently been recognized as potent regulators of reproduction in teleosts. Additionally, leptin plays an important role in energy homeostasis and reproductive function in teleosts. The purpose of this study was to examine differences in the concentration of the hormones of the Kiss/KissR system and leptin and the expression of their underlying genes, all of which are involved in the sexual maturation of female goldfish, Carassius auratus, following treatment with Kiss. The expression levels of KissR increased after the Kiss injection. Furthermore, the peptide hormone leptin also increased after the injection (in vivo and in vitro). Additionally, the expression of GnRH and GTHs (GTHα, FSHβ, and LHβ) increased in the brain and pituitary (in vitro and in vitro). These results support the hypothesis that Kiss plays important roles in the direct regulation of the hypothalamus-pituitary-gonad axis and leptin in goldfish. Therefore, we suggest that Kiss system gene expression is correlated with energy balance and reproduction.

• 한국수산과학회지
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• 제38권5호
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• pp.286-290
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• 2005

Oxytocin (OT)-related peptide, isotocin was purified from the brain extract of conger eel (Conger myriaster) using reverse-phase, ion-exchange and size exclusion high performance liquid chromatography (HPLC). The sequence of the peptide, with a molecular weight of 967.30 Da, was determined as Cys-Tyr-Ile-Ser-Asn­Cys-Pro-Ile-Gly-$NH_2$, where the Cys between 1st and 6th residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis and MALDI-TOF mass spectrometry. The sequence was confirmed by identical elution with the purified and synthetic peptide using the HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of OT -superfamily member, isotocin. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-7}M$ on the intestinal smooth muscle of goldfish (Carassius auratus).

• 한국수산과학회지
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• 제38권1호
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• pp.6-11
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• 2005

Vasopressin (VP)-related peptide was purified from the brain extract of conger eel (Conger myriaster) by reverse-phase, ion-exchange high performance liquid chromatography (HPLC). This peptide with a molecular weight of 1,051.2 Da was determined as $H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Arg-Gly-NH_2$, whose Cys residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis, MALDI- TOF mass spectrometry. It's sequence was confirmed by identity of the elution position with the synthetic peptide in HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of VP-superfamily member, $[Arg^8]-vasotocin$. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-10}\;M$ on the intestinal smooth muscle of goldfish.