DOI QR코드

DOI QR Code

Purification of Vasopressin-related Peptide, [Arg8]-vasotocin, from the Brain of Conger Eel (Conger myriaster)

붕장어 (Conger myriaster)의 뇌로부터 Vasopressin-related Peptide, [Arg8]-vasotocin의 정제

  • Go Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM In Hye (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Hee Yeon (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • HONG Yong-Ki (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 박희연 (국립수산과학원 생명공학연구단) ;
  • 윤호동 (국립수산과학원 생명공학연구단) ;
  • 홍용기 (부경대학교 수산과학대학 생물공학과) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • Published : 2005.02.01

Abstract

Vasopressin (VP)-related peptide was purified from the brain extract of conger eel (Conger myriaster) by reverse-phase, ion-exchange high performance liquid chromatography (HPLC). This peptide with a molecular weight of 1,051.2 Da was determined as $H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Arg-Gly-NH_2$, whose Cys residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis, MALDI- TOF mass spectrometry. It's sequence was confirmed by identity of the elution position with the synthetic peptide in HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of VP-superfamily member, $[Arg^8]-vasotocin$. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-10}\;M$ on the intestinal smooth muscle of goldfish.

Keywords

References

  1. Acher, R., J. Chauvet, M.T. Chauvet and D. Crepy. 1967. Amphibian neurohypophysial hormones: Isolation and characterization of mesotocin and vasotocin from the toad, Bufo bufo. Gen. Comp. Endocrinol., 8, 337-343 https://doi.org/10.1016/0016-6480(67)90082-2
  2. Acher, R., J. Chauvet and M.T. Chauvet. 1968. Neurohypophysial hormones of reptiles: isolation of mesotocin and vasotocin of the viper, Vipera aspis. Biochem. Biophys. Acta, 154, 255-257 https://doi.org/10.1016/0005-2795(68)90287-0
  3. Acher, R., J. Chauvet and M.T. Chauvet. 1970. Molecular evolution of the neurohypophysial hormones: The active peptides of a primitive bony fish Polypterus bichir. FEBS Lett., 11, 332-335 https://doi.org/10.1016/0014-5793(70)80561-0
  4. Bodanszky, M., H. Sharaf, J.B. Roy and S.I. Said. 1992. Contractile activity of vasotocin, oxytocin, vasopressin on mammalian prostate. Eur. J. Pharmacol., 216, 311-313 https://doi.org/10.1016/0014-2999(92)90376-F
  5. Chauvet, M.T, D. Hurpet, J. Chauvet and R. Acher. 1983. Identification of mesotocin, lysine vasopressin, and phenypressin in the eastern gray kangaroo (Macropus giganteus). Gen. Comp. Endocrinol., 49, 63-72 https://doi.org/10.1016/0016-6480(83)90008-4
  6. Chiu, K.W., Y.C. Lee and P.K.T. Pang. 1990. Neurohypophysial hormones and cardiac activity in the frog, Rana trigrina, and the snake, Ptyas mucosa. Gen. Comp. Endocrinol., 78, 150-154 https://doi.org/10.1016/0016-6480(90)90055-Q
  7. Conklin, D.J., M.P. Smith and K.R. Olson. 1999. Pharmacological characterization of arginine vasotocin vascular smooth muscle receptors in the trout (Oncorhynchus mykiss) in vitro. Gen. Comp. Endocrinol., 114, 36-46 https://doi.org/10.1006/gcen.1998.7233
  8. Cruz, L.J., V. de Santos, G.C. Zafaralla, C.A. Ramilo, R. Zeikus, W.R. Gray and B.M. Olivera. 1987. Invertebrate vasopressin/oxytocin homologs. Characterization of peptides from Conus geographus and Conus striatus venoms. J. Biol. Chem., 262, 15821-15824
  9. Feuerstein G., R.L. Zerbe and A.L. Faden. 1984. Central cardiovascular effects of vasotocin, oxytocin and vasopressin in conscious rats. J. Pharmacol. Exp. Ther., 228, 348-353
  10. Guibbolini, M.E. and B. Lahlou. 1987. Neurohypophysial peptide inhibition of adenylate cyclase activity in fish gills. FEBS Lett., 220 (1), 98-102 https://doi.org/10.1016/0014-5793(87)80883-9
  11. Lane, T.F., S.A. Sower and H. Kawauchi. 1988. Arginine vasotocin from the pituitary gland of the lamprey (Petromyzon marinus): Isolation and amino acid sequence. Gen. Comp. Endocrinol., 70, 152-157 https://doi.org/10.1016/0016-6480(88)90104-9
  12. Le Mevel, J.C., T.F. Pamantung, D. Mabin and H. Vaudry. 1993. Effects of central and peripheral administration of arginine vasotocin and related neuropeptides on blood pressure and heart rate in the conscious trout. Brain Res., 610, 82-89 https://doi.org/10.1016/0006-8993(93)91220-M
  13. Mahmoud, I.Y., R.V. Cyrus and D.L. Wright. 1987. The effect of arginine vasotocin and ovarian steroids on uterine contractility in the snapping turtle, Chelydra serpentina. Comp. Biochem. Physiol., 86A, 559-564
  14. Moore, G.J., J.A. Thornhill, V. Gill, K. Lederis and K. Lukowiak. 1981. An arginine vasotocin-like neuropeptide is present in the nervous system of the marine mollusc Aplysia californica. Brain Res., 206, 213-218 https://doi.org/10.1016/0006-8993(81)90119-0
  15. Nielsen, D.B., J. Dykert, J.E. Rivier and J.M. McIntosh. 1994. Isolation of Iys-conopressin-G from the venom of the worm-hounting snail, Conus imperialis. Toxicon, 32, 845-848 https://doi.org/10.1016/0041-0101(94)90009-4
  16. Noteborn, H.P.J.M., J.P.H. Burbach and I. Ebels. 1987. Modified forms of vasopressin and oxytocin in a bovine pineal preparation. FEBS Lett., 216, 200-206 https://doi.org/10.1016/0014-5793(87)80689-0
  17. Nozaki, M. and A. Gorbman. 1983. Immunocytochemical localization of somatostatin and vasotocin in the brain of the Pacific hagfish, Eptatretus stouti. Cell Tissue Res., 229, 541-550
  18. Oumi, T., K. Ukena, O. Matsushima, T. Ikeda, T. Fujita, H. Minakata and K. Nomoto. 1994. Annetocin: An oxytocin-related peptide isolated from the earthworm, Eisenia foetida. Biochem. Biophys. Res. Commun., 14, 393-399
  19. Pevet, P., A.C. Reinharz and J. Dogterom. 1980. Neurophysins, vasopressin and oxytocin in the bovine pineal gland. Neurosci. Lett., 16, 301-306 https://doi.org/10.1016/0304-3940(80)90015-4
  20. Proux, J.P., C.A. Miller, J.P. Li, R.L. Carney, A. Girardie, M. Delaage and D.A. Schooley. 1987. Identification of an arginine vasopressin-like diuretic hormone from Locusta migratoria. Biochem. Biophys. Res. Commun., 149, 180-186 https://doi.org/10.1016/0006-291X(87)91621-4
  21. Reich, G. 1992. A new peptide of the oxytocin/vasopressin family isolated from nerves of cephalopod Octopus vulgaris. Neurosci. Lett., 134, 191-194 https://doi.org/10.1016/0304-3940(92)90514-8
  22. Salzet, M., P. Bulet, A. Van Dorsselaer and J. Malecha. 1993. Isolation, structural characterization and biological function of a Iysine-conopressin in the central nervous system of the pharyngobdellid leech Erpobdella octoculata. Eur. J. Biochem., 217, 897-903 https://doi.org/10.1111/j.1432-1033.1993.tb18319.x
  23. Sawyer, W.H. 1967. Evolution of antidiuretic hormones and their functions. Am. J. Med., 42, 678-686 https://doi.org/10.1016/0002-9343(67)90088-5
  24. Takuwa-Kuroda, K., E. Iwakoshi-Ukena, A. Kanda and H. Minakata. 2003. Octopus, which owns the most advanced brain in invertebrates, has two members of vasopressin/oxytocin superfamily as in vertebrates. Regul. Pept., 115, 139-149 https://doi.org/10.1016/S0167-0115(03)00151-4
  25. Warne, J. 2002. The role of arginine vasotocin in teleost fish osmoregulation. Symp. Soc. Exp. Biol., 54, 83-95
  26. Warne, J.M., N. Hazen, J.C. Rankin and R.J. Balment. 1994. A radioimmunoassay for the determination of arginine vasotocin (AVT): plasma and pituitary concentration in fresh- and seawater fish. Gen. Comp. Endocrinol., 96, 438-444 https://doi.org/10.1006/gcen.1994.1200
  27. Wilson, N. and M. Smith. 1969. Isolation and amino acid sequence of neurohypophysial hormones of Pacific chinook salmon (Oncorhynchus tschwytscha). Gen. Comp. Endocrinol., 13, 412-424 https://doi.org/10.1016/0016-6480(69)90264-0

Cited by

  1. Purification of Oxytocin-related Peptide, Isotocin from the Brain of Conger Eel Conger myriaster vol.38, pp.5, 2005, https://doi.org/10.5657/kfas.2005.38.5.286