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http://dx.doi.org/10.5657/kfas.2005.38.5.286

Purification of Oxytocin-related Peptide, Isotocin from the Brain of Conger Eel Conger myriaster  

GO Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM In Hae (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
AN Sang Hyun (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
SOHN Hee-Young (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
PARK Jin-IL (Biotechnology Research, National Fisheries Research & Development Institute)
PARK HEE Yun (Biotechnology Research, National Fisheries Research & Development Institute)
YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute)
PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.38, no.5, 2005 , pp. 286-290 More about this Journal
Abstract
Oxytocin (OT)-related peptide, isotocin was purified from the brain extract of conger eel (Conger myriaster) using reverse-phase, ion-exchange and size exclusion high performance liquid chromatography (HPLC). The sequence of the peptide, with a molecular weight of 967.30 Da, was determined as Cys-Tyr-Ile-Ser-Asn­Cys-Pro-Ile-Gly-$NH_2$, where the Cys between 1st and 6th residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis and MALDI-TOF mass spectrometry. The sequence was confirmed by identical elution with the purified and synthetic peptide using the HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of OT -superfamily member, isotocin. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-7}M$ on the intestinal smooth muscle of goldfish (Carassius auratus).
Keywords
Brain; Conger myriaster; Conger eel; Contractile activity; Isotocin;
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