Browse > Article
http://dx.doi.org/10.5657/KFAS.2018.0383

Molecular Evidence of Cyprinid Herpesvirus 2 (CyHV-2) in Domestic Goldfish Carassius auratus and Imported Pearlscale Goldfish Carassius auratus  

Song, Hae Deok (Department of Aquatic life Medical Sciences, Sunmoon University)
Park, Jeong Su (Department of Aquatic life Medical Sciences, Sunmoon University)
Kwon, Se Ryun (Department of Aquatic life Medical Sciences, Sunmoon University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.4, 2018 , pp. 383-388 More about this Journal
Abstract
Goldfish hematopoietic necrosis (GHN) affects hematopoietic organs and causes high mortality in goldfish. The causative agent of GHN is cyprinid herpesvirus 2 (CyHV-2). The purpose of this study was to detect CyHV-2 in ornamental cyprinid fish. CyHV-2 monitoring was conducted on a monthly basis for 1 year using goldfish Carassius auratus and pearlscale goldfish Carassius auratus that had been cultured at a domestic aquafarm and imported from Singapore, respectively. The results of polymerase chain reaction (PCR) assays using specific primers for the CyHV-2 helicase gene showed that 27.8% of goldfish and 33.3% of pearlscale goldfish were positive for CyHV-2. No cytophatic effects were detected in koi fin or common carp brain cells inoculated with tissue homogenates from either fish. Our data provides the useful data for establishing future quarantine and disinfection policies.
Keywords
Goldfish; Pearlscale goldfish; Cyprinid herpesvirus 2; CyHV-2; Goldfish hematopoietic necrosis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ito T, Kurita J and Haenen OLM. 2017. Importation of CyHV-2-infected goldfish into the Netherlands. Dis Aquat Org 126, 51-62. http://dx.doi.org/10.3354/dao03157.   DOI
2 Jeffrey KR, Bateman K, Bayley A, Feist SW, Hulland J, Longshaw C, Stone D, Woolford G and Way K. 2007. Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK. J Fish Dis 30, 649-656. http://dx.doi.org/10.1111/j.1365-2761.2007.00847.x.   DOI
3 Jung SJ and Miyazaki T. 1995. Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.). J Fish Dis 18, 211-220. http://dx.doi.org/10.1111/j.1365-2761.1995.tb00296.x.   DOI
4 KMI (Korea Maritime Institute). 2010. Report of policy direction for a high value-added ornamental fish industry. KMI, Busan, Korea, 1-160.
5 MOF (Ministry of Oceans and Fisheries). 2016. Rapid growth of ornamental fish market [Internet]. Retrieved from http://www.mof.go.kr/article/view.do?menuKey=376&boardKey=10&articleKey=11102 on Feb 24, 2018.
6 NFPQMS (National Fishery Products Quality Management Service). 2016. Statistic database for fisheries import quarantine [Internet]. Retrieved from http://www.nfqs.go.kr/2013/contents.asp?m=5&s=8&s2=3 on Feb 24, 2018.
7 Ryu JW. 2015. First detection of Herpesviral haematopoietic necrosis virus [cyprinid herpesvirus 2 (CyHV-2)] from goldfish Carassius auratus in Korea. MS Dissertation, Chonnam National University, Chonnam, Korea.
8 Stephens FJ, Raidal SR and Jones B. 2004. Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus. Aust Vet J 82, 167-169. http://doi.org/10.1111/j.1751-0813.2004.tb12650.x.   DOI
9 Groff JM, LaPatra SE, Munn RJ and Zinkl JG. 1998. A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology. J Vet Diagn Invest 10, 375-378. http://dx.doi.org/10.1177/104063879801000415.   DOI
10 Waltzek TB, Kurobe T, Goodwin AE and Hedrick RP. 2009. Development of a Polymerase Chain Reaction Assay to Detect Cyprinid Herpesvirus 2 in Goldfish. J Aquat Anim Health 21, 60-67. http://dx.doi.org/10.1577/H08-045.1.   DOI
11 Wang L, He J, Liang L, Zheng X, Jia P, Shi X, Lan W, Xie J, Liu H and Xu P. 2012. Mass mortality caused by Cyprinid Herpesvirus 2 (CyHV-2) in Prussian carp (Carassius gibelio) in China. B Eur Assoc Fish Pat 32, 164-173. https://eafp.org/download/2012-volume32/issue_5/164-Wang.pdf.
12 Goodwin AE, Sadler J, Merry GE and Marecaux EN. 2009. Herpesviral haematopoietic necrosis virus (CyHV-2) infection: case studies from commercial goldfish farms. J Fish Dis 32, 271-278. http://dx.doi.org/10.1111/j.1365-2761.2008.0098.x.   DOI
13 Boitard PM, Baud M, Labrut S, De boisseson C, Jamin M and Bigarre L. 2016. First detection of Cyprinid Herpesvirus 2 (CyHV-2) in goldfish (Carassius auratus) in France. J Fish Dis 39, 673-680. http://dx.doi.org/10.1111/jfd.12400.   DOI
14 Chang PH, Lee SH, Chiang HC and Jong MH. 1999. Epizootic of herpes-like virus infection in goldfish, Carassius auratus in Taiwan. Fish Pathol 34, 209-210. http://dx.doi.org/10.3147/jsfp.34.209.   DOI
15 Goodwin AE, Khoo L, LaPatra SE, Bonar A, Key DW, Garner M, Lee MV and Hanson L. 2006. Goldfish haematopoietic necrosis herpesvirus (Cyprinid Herpesvirus 2) in the USA: molecular confirmation of isolates from diseased fish. J Aquat Anim Health 18, 11-18. http://dx.doi.org/10.1577/H05-007.1.   DOI