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http://dx.doi.org/10.5657/KFAS.2018.0383

Molecular Evidence of Cyprinid Herpesvirus 2 (CyHV-2) in Domestic Goldfish Carassius auratus and Imported Pearlscale Goldfish Carassius auratus  

Song, Hae Deok (Department of Aquatic life Medical Sciences, Sunmoon University)
Park, Jeong Su (Department of Aquatic life Medical Sciences, Sunmoon University)
Kwon, Se Ryun (Department of Aquatic life Medical Sciences, Sunmoon University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.4, 2018 , pp. 383-388 More about this Journal
Abstract
Goldfish hematopoietic necrosis (GHN) affects hematopoietic organs and causes high mortality in goldfish. The causative agent of GHN is cyprinid herpesvirus 2 (CyHV-2). The purpose of this study was to detect CyHV-2 in ornamental cyprinid fish. CyHV-2 monitoring was conducted on a monthly basis for 1 year using goldfish Carassius auratus and pearlscale goldfish Carassius auratus that had been cultured at a domestic aquafarm and imported from Singapore, respectively. The results of polymerase chain reaction (PCR) assays using specific primers for the CyHV-2 helicase gene showed that 27.8% of goldfish and 33.3% of pearlscale goldfish were positive for CyHV-2. No cytophatic effects were detected in koi fin or common carp brain cells inoculated with tissue homogenates from either fish. Our data provides the useful data for establishing future quarantine and disinfection policies.
Keywords
Goldfish; Pearlscale goldfish; Cyprinid herpesvirus 2; CyHV-2; Goldfish hematopoietic necrosis;
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