• 한국식품영양과학회지
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• 제33권4호
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• pp.705-710
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• 2004

$\varepsilon$-Polylysine에 grapefruit seed extract와 glyceride를 각각 혼합한 항균보존제의 식품부패균에 대한 항균효과를 MIC test와 실제 쌀밥에 적용하였다. 두 혼합 항균제 모두 clear zone을 나타내었으나, $\varepsilon$-polylysine과 grapefruit seed extract 혼합물이 $\varepsilon$-polylysine과 monoglyceride 혼합물보다 약 100배정도 낮은 minimum inhibitory concentration을 보였다. Glyceride 첨가제의 실험에서 Bacilius cereus와 Pseudomonas aerugines의 경우 0.1 $\mu$L/mL에서 생육이 저해된 것에 반해 Escherishia coli의 경우 15 $\mu$L/mL에 저해되어 E. coli가 이 항균제에 대하여 강한 저항성을 보이는 것으로 나타났다. 취반 시 glyceride 흔합항균제를 여러 농도별 (0.3%, 0.5%, 1%)로 첨가시킨 쌀밥을 72시간동안 저장하였을 때 총균수는 항균제의 농도가 증가할수록 감소하는 경향을 보였다. 쌀밥에 E. coli를 접종하여 항균제 농도에 따른 생육억제효과를 측정한 결과 항균제 농도가 높을수록 강하게 생육을 억제하였다. 항균제가 쌀밥의 관능에 미치는 영향은 맛, 냄새, 입안에서의 조직감에서는 유의적인 차이를 보이지 않았으나, 전체적인 기호도에서 1% 첨가군이 유의적인 차이를 보여 0.5%의 첨가가 적합한 것으로 나타났다.

• Elastomers and Composites
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• 제48권2호
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• pp.114-124
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• 2013

글리세라이드의 지방산 사슬길이 및 구조적 차이에 따른 영향을 평가하기 위하여 팜오일에서 추출된 글리세롤과 palmitic acid, stearic acid등 사슬길이가 $C_{12}{\sim}C_{18}$인 지방산을 이용하여, mono, di, tri등 다양한 글리세라이드 구조를 가진 신규 분산제를 합성하였고, 이를 실리카 충진배합물에 적용하여 기존의 금속염 분산제와 비교평가 하였다. 실험결과, 글리세라이드 분산제는 기존의 금속염 지방산 분산제 대비 혼합고무 내 윤활효과가 낮아서 혼합고무의 점도는 높으나, 실리카 분산이 크게 향상됨을 확인하였다. 이는 기계적 물성과 마모성능, Payne effect로 확인되었다. 또한 글리세라이드의 지방산 사슬길이가 길수록, 글리세롤에 중합된 지방산 수가 적을수록(tri- < di-< mono-glyceride)혼합고무 내 실리카 분산특성은 향상되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.300-306
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• 2000

This experiment was carried out to determine the contributions of acetate, glucose, amino acids and amino acid metabolites as carbon precursors for the incorporation of radioisotope, in intramuscular and subcutaneous adipose tissue and the effects of insulin on lipogenesis and adrenergic agent, norepinephrine on lipolysis in both tissues. The rate of incorporation of $C^{14}$ labelled acetate, glucose, leucine, isoleucine and ${\alpha}$-ketoisocaproic acid into adipose tissue has been measured in subcutaneous and intramuscular adipose tissues. The rate of incorporation was greater (p<0.05) from acetate than glucose in both subcutaneous and intramuscular adipose tissue and the rate of incorporation of carbon precursors into adipose tissues was greater in subcutaneous than in intramuscular adipose tissues. In comparison of amino acids, the rate was highest (p<0.05) with leucine followed by isoleucine and ${\alpha}$-ketoisocaproic acid in subcutaneous adipose tissue, in which there were no differences. Also, in intramuscular tissue, leucine was highest (p<0.05), and the rate of incorporation decreased in the same order. The rates of carbon precursor incorporation appeared to be higher in subcutaneous than in intramuscular tissue. For incorporation of radio-labelled acetate and glucose into intramuscular adipose tissue. preincubated for 48 hrs with insulin and IGF-1, insulin was the most effective to stimulate the incorporation of precursors in both substrates but there was no difference between insulin and IGF-1 in glucose incorporation. For glyceride-fatty acid synthesis, acetate was significantly (p<0.05) greater than glucose in both subcutaneous and intramuscular adipose tissue, and glyceride-glycerol synthesis was greater (p<0.05) for glucose than acetate in both adipose tissues. The rates of lipogenesis from both precursors were slightly greater in subcutaneous than intramuscular adipose tissue. There was significant (p<0.05) site effect in insulin treatment for glyceride-fatty acid synthesis. But there were no significance in control and norepinephrine. For glyceride-glycerol synthesis, there was no site effect caused by hormonal treatment. Insulin was the most effective (p<0.05) in glyceride fatty acid synthesis, while norepinephrine was the same as control. Compared with control, glyceride-glycerol synthesis from acetate in insulin treatment was significantly (p<0.05) low in subcutaneous, but high in intramuscular tissue. At the same time, in both tissues, it was lower in norepinephrine treatment than in control. Glyceride-glycerol synthesis from glucose was highest (p<0.05) in norepinephrine treatment followed by insulin although there was no significance between insulin and control. Lipolysis was not affected by insulin but was increased by norepinephrine when added to adipose tissue incubations in vitro. Rates of basal lipolysis were greater in subcutaneous adipose tissue than in intramuscular adipose tissue.

• Nutrition Research and Practice
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• 제2권2호
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• pp.130-133
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• 2008

This study was conducted to investigate the effects of lecithin, mono-glyceride and mono-diglyceride on apparent total tract and ileal nutrient digestibilities in nursery pigs. Twenty [(Landrace$\times$Yorkshire)$\times$ Duroc] barrows were surgically fitted with simple T-cannulas. Dietary treatments included 1) CON (basal diet: soy oil), 2) LO (lecithin 0.5%), 3) MO (mono-glyceride 0.5%), 4) MG (mono-glyceride 1.0%) and 5) MDG (mono-diglyceride 1.0%). In apparent total tract nutrient digestibility, dry matter (DM) and gross energy (GE) digestibilities of MDG treatments were higher than LO and MG treatments (p<0.05). In nitrogen (N) digestibility, LO treatment showed the lowest compared to others (p<0.05). The digestibility of crude fat was higher in MDG treatment than CON and LO treatments (p<0.05). In apparent ileal nutrient digestibility, DM digestibility was higher in MDG treatment than LO and MG treatments (p<0.05). GE digestibility was higher in MDG treatment than LO, MO and MG treatments (p<0.05). N digestibility of MDG treatment was greater than LO treatment (p<0.05). Also, the digestibility of crude fat was higher in MDG treatment than CON and LO treatments (p<0.05). In conclusion, mono-diglyceride can increase apparent total tract nutrient and apparent ileal nutrient digestibilities of DM, GE, N and crude fat.

• 한국식품영양과학회지
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• 제3권1호
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• pp.23-28
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• 1974

Fatty acids of Gingko biloba lipid and its binding position were determined by using pancreatic lipase. Optimum conditions for hydrolysis of glyceride were found as 9mg of lipase and 5 min reaction time for 50 mg of TG. The results showed that oleic acid and linoleic acid were presented about 40% and 29.7%, respectively, but linoleic acid was very small comparing with other seeds. It was found that both saturated and unsaturated fatty acids were almost equally distributed at ${\beta}\;and\;{\alpha}{\cdot}{\alpha}'-position$ of TG.

• KSBB Journal
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• 제13권1호
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• pp.90-95
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• 1998

Candida cylindracea유래의 리파제인 lipase-OF 360,000를 이용한 다중불포화지방산의 농축공정개발에 관한 연구를 수행하 였다. Lipase-OF 360,000은 5가지 종류에 대한 유지의 가수분해에서 다중불포화지방산인 n-3족 아실기를 다량 함유하는 물고기기름에 대해서만 낮은 활성을 보였다. 이 리파제에 의한 물고기기름의 가수분해에서 반응이 진행됨에 따라 DHA는 계속 농축되는 양상을 보이지만 EPA의 경우는 가수분해반응이 0-30% 진행시까지는 완만하게 농축이 진행되다가 30-50% 진행되는 동안은 더 이상의 농축이 진행되지 않았고 약 50% 이상부터는 감소하기 시작하여 반응 완료시에는 반응전 물고기기름에 있어서 EPA의 함량과 거의 비슷한 약 18% 정도로 감소되었다. 이러한 양상은 기질에 대한 Lipase-OF 360,000의 농도에 상관없이 거의 유사하게 일어났다. 본 연구결과로서 Lipase-OF 360,000의 아실 체인 특이성을 두 가지로 요약할 수 있는데 첫 번째는 가수분해에 있어서 물고기기름내의 n-3족 다중불포화지방산과 그 외의 다른 지방산들의 차별성이다. Lipase-OF 360,000은 n-3족 다중불포화지방산에 대해서만 현저히 낮은 활성을 보였다. 두 번째는 n-3족 다중불포화지방산이 가지는 구조적 차이가 Lipase-OF 360,000의 특이적 활성에 미치는 영향이다. 이 경우는 n-3족 다중불포화지방산내의 탄소수와 불포화도가 높을수록 Lipase-OF 360,000의 활성이 좋지 않았다.

• 한국식품영양학회지
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• 제4권1호
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• pp.21-34
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• 1991

The purposes of this studies were to investigate the effect of dietry Se levels and alcohol administration on the lipid hyperoxidation and the lipid metabolism in the rat. Seventy two male rats of Sprague-Dawley Strain weighting about 58~629 were divided into 12groups. The dietary Se levels were 10, 0.4 and Omg, and the dietary a-tocopherol levels were 150 and 0mg per kg diet, respectively. Alcohol-administrated groups received drinking water solution containing 10% of ethanol from the 3-weeks of experimental periods. The obtained experimental results are summarized as follows. 1. Food intake, body weight gain and food efficiency ratio were significantly lower in H-, L-and alcohol administrated groups(-A) by administrated Se and alcohol in diet. The weight of liver and spleen tended to be greater in H- and alcohol administrated groups. 2. The glutathione values in liver tend to be lower in alcohol or Vit. E administrated groups than nonadministrated groups. Also there were higher in H- and L- than C-groups, but the increasing range decreased due to administrated alcohol. The lipid peroxide values In liver were significantly higher in alcohol groups, and L- and tocopherol groups were higher values. Specially the increasing of lipid peroxide values were significantly effected by alcohol in low Se and Vit. E groups. 3. The contents of total glyceride in plasma were higher in alcohol groups, there were significantly higher values in alcohol administrated groups under low Se and Vit. E groups. The contents of total cholesterol and HDL-cholesterol In plasma were significantly higher in alcohol groups. 4. The contents of total lipid in liver were higher alcohol groups, and slightly higher values in low Se groups(L-groups) than other groups, also higher values in low Vit. E groups. Those of total glyceride in liver were significantly higher in alcohol groups, appeared highest values when alcohol was administrated in low Se and Vit. E groups. The increasing of total glyceride content was significantly effected by alcohol in low Se groups than that in C-groups.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.312-312
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• 2005

• Journal of Ginseng Research
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• 제23권1호
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• pp.8-12
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• 1999

알코올에 중독된 실험동물에 홍삼 산성다당류(AcPS)를 4주간 투여하며 성장지표를 비교해 본 결과, 알코을 중독시에 나타나는 영양 및 대사의 파괴를 막아주거나 경감시켜 지방간 및 간장비대 상태를 크게 호전시켰다. 혈중 지질대사 요소인 혈중 총콜레스테롤, HDL-cholesterol 및 중성지질의 농도는 정상치에 가깝게 낮추어 고지혈증상을 유의적으로 개선시킴을 확인할 수 있었으며, 혈중농도와 마찬가지로 간장조직중 농도에 미치는 영향도 신뢰성있는 개선 효과를 인정할 수 있었으며, 이러한 효과는 AcPS의 투여기간($1\~4$주)에 비례하는 것으로 나타났다.

• 한국약용작물학회지
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• 제7권2호
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• pp.107-114
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• 1999

미나리는 간을 보호하며 간질환을 예방, 치유하는 효능이 있는 기능성식물로 인식되어 민간요법에 널리애용되고 있다. 그래서 알콜을 렛트와 마우스에 처리하여 만성, 아급성, 급성중독을 유도하고 밭미나리 발효액과 추출물 그리고 물미나리추출물의 효과를 조사한 결과는 다음과 같다. 렛트와 마우스에 에탄올을 2일동안 투여한결과 total cholesterol과 total glyceride의 수치가 증가되었으며 20일동안 밭미나리발효액과 추출물 그리고 물미나리추출물을 투여한후 에탄올을 2일동안 급여한균에서는 total cholesterol과 total glyceride의 감소 효과가 있었으나 처리간의 유의성은 인정되지않았다. 그러나 실험동물의 혈청중에 알콜에의해서 높아졌던 alanine aminotransferase 와 asparate aminotransferase는 밭미나리발효액 과 추출물에 의해서 감소되었으며 밭미나리발효액투여군에서 가장 많이 감소되어 처리간에 유의성이 인정되었다. 렛트에 10%에탄올을 10개월간 투여하여 만성알콜중독을 유발시킨다음 5일동안 물만 급여한군과 밭미나리발효액을 투여한군을 비교한바 total cholesterol은 40%, total glyceride는 60%의 감소효과가 있었으며 alanine aminotransferase 와 asparate aminotrasferase는 87.2%와 91.7%를 감소시켜 정상에 가깝게 회복시키는효과가 인정되었다. alkaline phosphatase, catalase, superoxide dismutase 및 glutathione peroxidase의 활성은 알콜의 처리에 의해서 급만성모두 유의적인 변동을 나타냈으며 밭미나리 발효액의 처리에 의해서 alkaline phosphatase와 superoxide dismutase의 활성에 특히 유의적인 감소를 가져왔다. 이와같은 결과는 물미나리보다 밭미나리발효액이 알콜성 간질환에 효능이있음을 시 사하는것이라 하겠다.