• 한국식품영양과학회지
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• 제37권11호
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• pp.1401-1407
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• 2008

본 연구는 $\alpha$-glucosidase의 강력한 효소저해제인 1-deoxynojirimycin(DNJ)을 고효율로 생산하는 균주의 선발 및 동정, 배양최적화 및 균 배양액의 혈당강하효과 등에 관하여 조사하였다. 먼저 토양으로부터 $\alpha$-glucosidase에 대해 강력한 저해능을 나타내는 9균주를 대상으로 이 중에서 DNJ 생산성이 가장 우수한 한 개 균주를 최종 선발하였고, 이 균주를 16S rDNA 염기서열분석으로 균주동정을 하여 Bacillus subtilis S10이라 명명하였다. 본 균주의 배양액을 이온교환수지법(Amberlyst 15 $H^+$ form, Dowex $1{\times}2$-100 $OH^-$ form, Amberlite CG-50 $NH_3^+$ form)에 의해 DNJ를 정제하고, 정제된 DNJ를 LC-MS로 분석한 결과 표준 DNJ와 동일한 물질임을 확인하였다. DNJ 대량생산을 위한 S10균주의 배양 최적탄소원 및 최적질소원과 이들의 최적농도를 조사한 결과 각각 1% galactose, 1.6% polypeptone임이 밝혀졌으며, 확립된 최적화 조건에서의 DNJ 생산량은 0.75 g/L이었다. 고혈당 유도 마우스를 대상으로 각종 혈당강하제의 효과를 알아보기 위해 혈당치를 비교한 결과 무처리구의 혈당치 $510{\pm}10.9\;mg/dL$에 비해 acarbose 처리구는 $139.4{\pm}33.1\;mg/dL$, 누에분말 처리구는 $209.1{\pm}19.6\;mg/dL$, 그리고 S10 균주 배양액 처리구는 $208.6{\pm}39.8\;mg/dL$로써 무처리구에 비해 각각 72.7%, 59.0%, 그리고 59.1%의 혈당 억제율을 나타냈다. 이상의 연구결과 S10균주 배양액은 누에분말과 같은 수준의 고혈당 억제 및 완화효과가 있음이 확인되었으며 향후 S10균주의 대량생산을 통해 식후혈당조절을 위한 건 강기능식품 개발이 기대된다 하겠다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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• pp.53-53
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• 2003

Alpha-glucosidase inhibitor, 1-deoxynojirimycin, has been shown to eliminate the production of ER-budding viruses in the ER of host cell. This glucose-derived iminosugar derivative, 1-DNJ, has been largely purified from midgut of silkworm, Bombyx mori, Here, we have screened the antiviral activity in the GBV-B infected marmoset hepatocyte cell line. (omitted)

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1003-1008
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• 2009

The $O_sO_4$-catalyzed dihydroxylations of a monosubstituted allylic amine and $\gamma-amino-\alpha,\;\beta-unsaturated$ (E)-esters with bulky alkyl groups showed a high anti-selectivity. Since the acyclic conformation of N-acyloxy protected allylic amines was efficiently controlled by a bulky t-Bu or OBO ester group, the anti diastereoselectivity of >12.5:1 was obtained without applying a chiral reagent. The synthetic utility of the present method was demonstrated by a stereoselective and efficient synthesis of an $\alpha$-glucosidase inhibitor 15 from commercially available N-Cbz-L-serine 6 in 11 steps and 31% yield.

• 당뇨병
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• 제16권3호
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• pp.161-167
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• 2015

In Korea, diabetes mellitus, which causes micro and macrovascular complications, has been rapidly increasing during recent decades and has become a leading cause of disability-adjusted life years. The prevalence of diabetes mellitus was 12.4% in 2011 and that of prediabetes, a condition of high risk for developing diabetes mellitus, is 1.5- to 3-fold greater than that of diabetes. The diabetes prevention programs in other countries were shown to reduce or delay progression from prediabetes to diabetes mellitus. However, these results are not applicable to Korean people because of genetic and environmental differences. Therefore, we need to plan and perform a diabetes prevention study in Korean. Based on these results, we should design intervention tools for a Korean diabetes prevention program. We can consider several preventive interventions with lifestyle modification suitable for Korean people and pharmacologic treatments such as metformin or alpha-glucosidase inhibitor.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.546-552
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• 2005

The purpose of this study was to determine the effect of rat intestinal ${\alpha}-glucosidase$ inhibitor ; methanol(80%), ethanol(80%) and $dH_2O$ extract of Schizandra chinensis in Korea(KS : Schizandra chinensis in Korea) and China(CS : Schizandra chinensis in China). When the final concentration was 1 $mg/m{\ell}$ for each sample(KS and CS), MeOH extract of KS($IC_{50}$ 1.62 mg/ml) showed 46.8%, EtOH extract of KS($IC_{50}$ 1.48 mg/ml) showed 47.4%, $dH_2O$ extract of KS($IC_{50}$ 1.72 mg/ml) showed 46.3% and MeOH extract of CS($IC_{50}$ 8.35 mg/ml) showed 13.3%, EtOH extract of CS($IC_{50}$ 8.05 mg/ml) showed 16%, $dH_2O$ extract of CS($IC_{50}$ 8.37 mg/ml) showed 11.54% of inhibiter for p-nitrophenyl ${\alpha}$, D-glucopyranoside ${\alpha}-glcosidase$ activity, respectively, And the contents of total phenol, flavonoid of Schizandra chinensis were measured. When the final concentration was 1 $mg/m{\ell}$ for each sample(KS and CS), total phenol and flavonoid in KS were higher than CS, respectively.

• 한국식품영양학회지
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• 제30권6호
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• pp.1222-1228
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• 2017

In the current study, we investigated the inhibitory activity of water soluble ${\beta}-glucan$ from oat (Avena sativa) against various digestive enzymes such as ${\alpha}-glucosidase$, sucrase, maltase and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can significantly decrease the post-prandial increase of blood glucose level after a mixed carbohydrate diet. The ${\beta}-glucan$ had the highest documented rate of small intestinal sucrase inhibitory activity (2.83 mg/mL, $IC_{50}$) relevant for potentially managing post-prandial hyperglycemia. Furthermore, we evaluated the effects of ${\beta}-glucan$ on the level of post-prandial blood glucose in animal model. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without ${\beta}-glucan$ (100, and 500 mg/kg-body weight). The maximum blood glucose levels (Cmax) of ${\beta}-glucan$ administration group were decreased by about 23% (from $219.06{\pm}27.82$ to $190.44{\pm}13.18$, p<0.05) and 10% (from $182.44{\pm}13.77$ to $165.64{\pm}10.59$, p<0.01) in starch and sucrose loading test, respectively, when compared to control in pharmacodynamics study. The ${\beta}-Glucan$ administration significantly lowered the mean, maximum, and minimum level of post-prandial blood glucose at 30 min after meal. In view of the foregoing, it is felt that our findings suggest that ${\beta}-glucan$ from oat serves to reduce post-prandial blood glucose rise secondary to slower absorption of glucose in the small intestine, via carbohydrate hydrolyzing enzymes inhibition.

• 한국미생물·생명공학회지
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• 제42권4호
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• pp.402-406
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• 2014

본 연구에서는 아직까지 우리나라 미생물관련 학술지에 보고되어 있지 않은 Rhodosporidium diobovatum SY4-2, Cryptococcus bestiolae SY7-1, Kazachstania unispora SY14-1, Kazachstania servazzii SY14-3, Pichia holstii SY20-2와 Cryptococcus tephrensis SY26-1 등 6종의 새로운 국내 미기록 효모들을 전라북도 고군산열도의 선유도 일대 야생화들에서 분리, 동정된 61균주에서 선별하였다. 이들 균주들 모두 구형과 난형으로 50% 포도당을 함유한 YPD 배지에서 잘 생육하였고, Pichia holstii SY20-2는 20% NaCl을 함유한 YPD 배지에서 생육하는 극도 호염성 효모이었다. Cryptococcus tephrensis SY26-1은 포도당을 자화시키지 못하였으나 Cryptococcus bestiolae SY7-1과 함께 유당을 자화시켰다. 6종의 미기록 효모 균주들의 생리기능성을 조사한 결과 이들 균주들의 무세포추출물들의 항당뇨성 ${\alpha}$-glucosidase 저해활성이 86.8-98.6%로 매우 높았고, 특히 Kazachstania servazzii SY14-3의 무세포추출물이 98.6%로 가장 높았다. 또한, 미기록 효모 균주들의 배양상등액의 항고혈압성 안지오텐신 전환효소 저해활성도 58.6-80.4%로 비교적 높았다.

• 생명과학회지
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• 제32권8호
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• pp.589-594
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• 2022

이 연구에서 베툴린산이 STZ에 의해 유발된 고혈당 쥐에서 탄수화물 소화 효소의 활성을 억제하고 식후 고혈당을 감소시킬 수 있는지 여부를 알아보았다. 그 결과, 베툴린산이 α-글루코시다아제와 α-아밀라아제 활성에 강력한 억제 효과를 보여주었다. α-글루코시다아제와 α-아밀라아제에 대한 베툴린산의 IC₅₀는 각각 12.83± 6.81 및 18.32±3.24 μM으로, 이는 경구 혈당강하제인 acarbose의 IC₅₀ 보다 값이 낮아 베툴린산의 탄수화물 소화효소의 억제 활성이 높다는 것을 나타낸다. 당뇨쥐와 정상쥐에서 증가된 식후 혈당은 베툴린산을 투입한 고혈당군, 정상군 모두 대조군보다 유의하게 식후 혈당이 억제되었습니다. 30, 60, 120분에 각각 혈당을 측정하였을 떄, 당뇨쥐에서 베툴린산을 투여한 군의 혈당은 23.22±1.1, 24.38±1.31, and 21.05±1.36 μM 으로 대조군의 혈당인 24.64± 1.7, 27.22±1.58, and 26.36±1.40 μM 보다 유의하게 감소하였다. 당뇨쥐에서 베툴린산 투여한 군의 AUC도 대조군 쥐에 비해 유의하게 감소하였지만, acarbose를 투여한 군에 비해서는 더 많이 감소하지 않았다. 이러한 연구 결과는 베툴린산이 탄수화물 소화 효소의 강력한 억제제로의 가능성을 보여주어, 당뇨병 쥐의 식후 고혈당증을 개선할 수 있을 것이라 사료된다.

• 대한의생명과학회지
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• 제11권4호
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• pp.517-523
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• 2005

The purpose of this study was to determine the effect of rat intestinal a-glucosidase inhibitor; methanol $(80\%)$, ethanol $(80\%)$ and water extract of Schizandra chinensis in Korea (KS: Schizandra chinensis in Korea) and China (CS: Schizandra chinensis in China). When the final concentration was 1 mg/ml for each sample (KS and CS), methanol extract of KS ($IC_{50}$ 1.62 mg/ml) showed $46.8\%$, ethanol extract of KS ($IC_{50}$ 1.48 mg/ml) showed $47.4\%$, water extract of KS ($IC_{50}$ 1.72 mg/ml) showed $46.3\%$ and methanol extract of CS ($IC_{50}$ 8.35 mg/ml) showed $13.3\%$, ethanol extract of CS ($IC_{50}$ 8.05 mg/lml) showed $16\%$, water extract of CS ($IC_{50}$ 8.37 mg/ml) showed $11.54\%$ of inhibitor for p-nitrophenyl $\alpha-D-glucopyranoside$ (pNPG) $\alpha-glcosidase$ activity, respectively. And the contents of total phenol, flavonoid of Schizandra chinensis were measured. When the final concentration was 1mg/ml for each sample (KS and CS), total phenol and flavonoid in KS were higher than CS, respectively. The order superoxide dismutase (SOD) activity $IC_{50}$ values of each solvent extracts of KS were: 2.006 mg/ml methanol extract, 2.304 mg/ml ethanol extract and 2.5 mg/ml water extract, which were higher than that of each solvent extracts CS as: 2.881 mg/ml methanol extract, 3.085 mg/ml ethanol extract and 3.190 mg/ml water extract.

• Journal of Microbiology and Biotechnology
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• 제7권4호
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• pp.262-266
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• 1997

To increase the high production of 1-deoxynojirimycin (DNJ) from Streptomyces sp. SID9135, the effect of various carbon sources, nitrogen sources, cationic metal ions, the initial pH of the medium, and agitation speed were investigated. The most effective carbon and nitrogen sources were found to be lactose 2.5% (w/v) and soybean meal 2.0% (w/v), respectively. None of the cationic metal ions examined had any detectable stimulating effect on DNJ production except $Fe^{+2}$ ion. The initial optimum pH for DNJ production ranged from 6-8 and agitation speed was most effective at 400 rpm. In the jar fermentor experiments under optimal culture conditions, the accumulation of DNJ reached about $640{\mu}g$/ml after 5 days of cultivation and the level remained the same for a further two days.