• The Korean Journal of Pesticide Science
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• v.20 no.2
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• pp.83-92
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• 2016

New tree wound-treatment formulations (WTF) were developed in this study. Stimulating effects of plant growth regulating substance on callus formation were evaluated in vivo twig disc culture of Zelkova serrata, Ziziphus jujuba, Pinus densiflora, Ginkgo biloba. and Aesculus turbinata. Based on the evaluation, WTF were prepared with 2,4-D, NAA, and IBA using xanthan gum as an extending agent. WTF were pasted on $2{\times}10cm$ artificial wound on the stem and the effects were evaluated 12 weeks later with the thickness of new callus-tissues. The effects varied with tree species and the growth regulators. In Z. serrata and A. turbinata, 2 mg/L of 2,4-D was the best WTF. In P. koraiensis and G. biloba, 8 mg/L of NAA and 1 mg/L of IBA were the best, respectively. Callus formation was quite lower in G. biloba compared to other species. Stimulating effect of thiophanate-methyl paste was excellent only in Z. serrata and lower than certain WTF in other species. Vaseline, which was used generally as an WTF, remained longer on the wound and causing decay. From the results, it could be recommended that 2,4-D, NAA and IBA were the best WTF for Z. serrata/A. turbinata, P. koraiensis and G. biloba, respectively.

• Journal of Korean Society of Forest Science
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• v.77 no.1
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• pp.43-52
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• 1988

One-year-old seedlings of Ginkgo biloba were treated with various simulated acid rains(pH 2.0, pH 3.0, pH 9.0 and pH 5.0) to examine the effects of simulated acid rain on the chemical properties of the tested soil. The seedlings were grown in a pot($4500cm^3$)containing one of three different soils(nursery soil, mixed soil and sandy soil). Simulated acid rain was made by diluting sulfuric and nitric acid solution($H^1SO^4$ : $HNO^3$ =3 : 1, V/V) with tap water and tap water(pH 6.4), and treated by 5mm each time for three minutes during the growing seasons(April to October 1985). Acid rain treatments were done three times per week to potted seedlings by spraying the solutions. The chemical properties of potting media were compared among three soil types as well as among the various pH levels. The results obtained in this study were as follows : 1. Exchangeable calcium and magnesium contents and base saturation of the soil decreased with decreasing pH levels of acid rain, and their decreasing rates were as follows : sandy soil was the highest, followed by mixed and nursery soils, However, exchangeable aluminum content rather increased as the pH levels decreased. 2. Available phosphate in the soil decreased as the pH levels of acid rain decreased. Its content increased in nursery soil, compared with those before acid gain treatment, Gut decreased in mined and sandy soils. 3. Soil sulfate and nitrate contents increased remarkably as the pH levels decreased, and the only significant difference in the sulfate was found among the pH levels. Soil sulfate content was the highest in nursery soil, followed by mixed and sandy soils.

• Journal of Korean Society of Forest Science
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• v.76 no.3
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• pp.230-240
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• 1987

Half-sib seedlings of Ginkgo biloba (one-year-old) were treated with various simulated acid rains(pH2.0, pH3.0, pH4.0 and pH5.0) to examine the effects of acid rain on leaf surface area, leaf injury, leaf chlorophyll content and photosynthetic ability of the leaf tissue. The seedlings were grown in a pot($4500cm^3$) containing one of three different soils(nurseryy soil, mixed soil and sandy soil). Simulated acid rain was made by diluting sulfuric and nitric acid solution($H_2SO_4:HNO_3=3:1$, V/V) with tap water and tap water(pH6.4), and treated by 5mm each time for three minutes during the growing seasons(April to October 1985). Acid rain treatments were done three times per week to potted seedlings by spraying the solutions. The results obtained in this study were as follows : 1. Leaf surface area per seedling at pH2.0 level was the lowest among the levels of pH, but those at other pH levels were not significantly different. 2. Leaf injury(injured leaf rate and injured leaf area) increased with decreasing pH levels of acid rain. 3. Leaf chlorophyll content measured during the period June through October was significantly different among the soil types, and that of the seedling in nursery soil was the highest. The lower pH levels of simulated acid rain was treated ; more leaf chlorophyll content was measured at the beginning of treatment, and the more it severely decreased at the late growing period. 4. Photosyntetic abilities, and the highest value was shown in nursery soil. Significand difference in photosynthetic ability among the levels of pH was observed only in August. Photosynthetic ability increased with decreasing pH levels at the beginning of treatment, but decreased rapidly after July.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.2
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• pp.141-147
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• 2005

This study investigated air pollutant levels and physiological variation of Ginkgo biloba in Chuncheon. The results were as follows: The annual average concentrations of $SO_2,\;NO_2\;and\;PM10$ were 0.004ppm, 0.013 ppm and $66{\mu}g/m^3$, respectively. The volume weighted average concentrations of ionic components were $SO_4\;^{2-}\;3.584 mg/m^3,\;NO_3^-\; 2.803 mg/m^3,\;Cl^-\;1.485 mg/m^3\;and\;NH_4\;^+\;0.998 mgg/m^3$ in precipitation. The annual wet deposition amount of the major ions was shown to be $SO_4^{2-}\;3.865g/m^2/yr,\;NO_3^-\;2.924g/m^2/yr,\;Cl^-\;2.773g/m^2/yr\;and\; NH_4\;^+\;1.485 g/m^2/yr$ during this study period. The seasonal averaged pH in leaves were spring pH 5.9 0.5, summer pH 5.5 0.4 and fall pH 5.1 0.3. The seasonal average water soluble sulfur content in leaves were spring 0.012 0.004%, summer $0.012\;0.002\%\;and\;fall\;0.020\;0.007\%$. The seasonal average water soluble sulfur content in bark were spring $0.0071\;0.0003\%,\;summer\; 0.0066\;0.0004\%,\;fall\;0.0063\;0.0004\%\;and\;winter\;0.0071\;0.0003\%$.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.5
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• pp.1025-1034
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• 2002

The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by behavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed (～100%), whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mANA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidylethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.6
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• pp.1-11
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• 2004

This study was conducted to investigate the plants status of the landscape plant species of 10 elementary schools in the Chungju city, and to obtain the fundamental informations for proper use of planting system in the elementary school landscape. The results are summarized as follows : 1. 199 species were planted in the 10 elementary schools, It was constituted 89 of woody plants, 108 of herbaceous plants and 2 of others. The average number of plants per school was about 60 species. The rate of plants species of Evergreen Trees to the Deciduous was 23 : 60 and the rate the trees to the shrubs was 59 : 24. 2. Almost the same species were planted in school landscaping zone. Juniperus chinensis var. kaizuka, Ginkgo biloba, Zelkova serrata were mainly planted in the species of trees. To improve the present conditions of school landscape should be considered for the purpose of function and the aspect of ecology. 3. To see the selected school trees and flowers, Zelcova serrata was selected to the school tree in the 4 of schools. Ginkgo biloba was selected 3 of schools, the others were Juniperus chinensis Pinus densiflora, etc. Rhododendron lateritium was appointed to the school flower in the 6 of schools, the others were Rosa spp., Forsythia koreana etc. To set the school tree and flower consider the preserved trees of the community.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.895-905
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• 2004

Herb found in the farm of Korea were classified into 3 divisions, 5 classes, 31 orders, 51 families, 134 genera and 226 kinds. In the taxon of family, labiatae, compositae and umbelliferae have larser number of herbs than another families. In the taxon of genus, Rosa (34 kinds), Mentha (8), Thymus (7), Pelargonium (6), Salvia (6), Lavandula (5), Citrus (4), Ocimum (4), Allium (4) in order contain many species. In the geographical floristic regions, Holarctic were 149 kinds, and next came Paleotropical of 26 kinds, Neotropical of 18 kinds, Capetown of 8 kinds, and Australian of 6 kinds in order. Plants growing naturally over 3 geographical region were 4 kinds including Passiflora caerulea. And 166 kinds were growing in the only 1 geographical region. Among the all farms, (1) farm (in Cheongwon) were highest number of 137 kinds. On the other hand, (3) farm (in Cheongsong) were lowest number of 59 kinds. Herb cultivating in all farms were 37 kinds including Pelargonium crispum. Among these, plants of 24 kinds belong to the labiatae family. Also, herb cultivating in the only 1 farm were 50 kinds including Humulus lupulus. About the numbers of usage of herb, the first in numbers was for food $(54.4\%,\;122\;kinds)$ including Ginkgo biloba, the second for medicinal purpose $(39.8\%,\;90\;kinds)$ including Equisetum arvense, the third for cosmetic $(15.9\%,\;36\;kinds)$, th fourth for flavor $(14.2\%,\;32\;kinds)$, the next for perfume $(12.4\%,\;28\;kinds)$, the next for dye $(10.8\%,\;23\;kinds)$, the next for insecticide $(6.6\%,\;15\;kinds)$, the next for ornament $(1.8\%,\;4\;kinds)$, the last for antiseptic $(0.9\%,\;2\;kinds)$.

• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.832-846
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• 2020

The purpose of this research was to evaluate the vapor permeability of nine different species of domestic softwood and the vapor permeability evaluation through the production of small wall structures for the developing applications, utilizing the vapor permeability of wood. In addition, the permeability evaluation was attempted by means of the production of a small wall structure injected with a waste material, bottom ash, as a moisture absorbent for improving the permeability. Consequently, the results of the vapor permeability evaluation by means of S_d value are as follows: (1) It was observed that Abies holiphylla, Picea jezoensis, Ginkgo biloba, Pinus koraiensis and Pinus rigida are permeable to moisture among 9 species of domestic softwood in Korea. (2) By means of this, semi-permeability efficiency was evaluated when producing a small wall structure. (3) Besides, improved effects of permeability were evaluated when producing a small wall structure inserted with bottom ash. As a result, it was confirmed that the S_d value of Pinus koraiensis turned out to be 1.63, which is superior to other 8 tree species.

• Journal of Periodontal and Implant Science
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• v.25 no.3
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• pp.478-486
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• 1995

Periodontal therapy for treatment of periodontitis involves the elimination of bacterial plaque and elimination of the anatomic defects by regenerative procedure. The purpose of this study was to evaluate on the biological effect of magnolia and Ginkgo biloba extract to the antimicrobial, antiinflammatory and cellular activity. Antimicrobial assay was performed with the diffusion method of the extract by measuring of growth inhibitory zone of B. cereus from blood agar plate. Effect of the extract to cellular activity of gingival fibroblast were examined using MTT method and measured the result with optical density on 570nm by ELISA reader. Inhibitory effects of $PGE_2$ production from gingival fibroblast was performed with the addition of $IL-l{\beta}$ and the extract to the well and examined to the product of $PGE_2$ from cell by ELISA reader. In vivo anti-inflammatory effect was performed with injection examined with clinically and histologically for their extent of mecrosis and inflammation. Antimicrobial activity of Magnolia extract showed significantly higher activity than that of control. However, GBE did not showed significant activity to compare with control, and mixture of Magnolia and GBE extract showed significantly higher activity than that of control. The effect of cellular activity to gingival fibroblast showed no significant differences of between control and Magnolia extract. However, GBE showed significantly higher rate of cellular activity to compare with control and even to PDGF-BB, and also showed same degree of cellular activity even though mixed with Magnolia extract. The inhibitory effect of $PGE_2$ production showed significantly reduction of $PGE_2$ production to compare with control, but its inhibitory effect was not much strong to compare with Indomethacin. In vivo, antiinflammatory effect of Magnolia extract to P. gingivalis injection of Hamster buccal check showed significantly reduction of inflammatory cell infiltration and tissue necrosis, but GBE showed no effect on the inhibition of inflammatory process. These results suggested that Magnolia and GBE extract possessed different kind of biological activity and also can be compensated on their activity with each other for elimination of bacterial plaque and anatonical defect.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.328-336
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• 1993

This study was conducted to investigate sensitivity of tree seedlings to $SO_2$. Visible injury symptoms and changes of ethylene production were investigated in tree seedlings with the fumigation of $SO_2$ in gas chamber 4 hours a day for six days. The symptoms of visible injury did not appear below 0.5ppm level of $SO_2$ exposure but a change of visible injury with the passage of time appeared at 1.5 and 2.5ppm in all seedlings. With the higher the concentration and/or the longer exposure of $SO_2$ the visible injury symptoms on leaves increased in all seedlings. The sensitivity of seedlings to $SO_2$ was the highest in Liriodendron tulipifera followed by Pinus strobus, Ginkgo biloba, Pinus densiflora and Pinus koraiensis. The amount of ethylene production was more at 1.5 and 2.5ppm of $SO_2$ exposure than at 0.5ppm and the peak time of it came faster at higher levels. The amount of ethylene production was significantly different among tree seedlings. It showed a higher at production of ethylene in Liriodendron tulipifera compared to Ginkgo biloba and the ethylene production of Pinus trees to $SO_2$ were the highest in Pinus strobus followed by Pinus densiflora and Pinus koraiensis. In needle of Pinus strobus the ethylene production increased with the increasing rate of visible injury until the injury rate of 40-50% and than decreased with the increasing rate of visible injury since the rate of 50%.