• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2001.10b
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• pp.162-162
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• 2001

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.231.1-231.1
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• 2000

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.4
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• pp.341-344
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• 1997

To check the possibility to use the pharmaceutical by product of ginkgo leaf for the improvement soil fertility, a pot experiment was conducted with paddy rice. There were three treatment; NPK alone, NPK+7000kg(air dry)/ha of ginkgo leaf waste and NPK+700 kg of water-washed ginkgo leaf waste (air dry)/ha. The result indicated that the application of ginkgo leaf waste severely retarded the growth of rice. Water washing did reduce the severity of retardation, but the water washed ginkgo leaf waste also retarded the growth of rice significantly. The result of this study suggested that ginkgo leaf waste may contain some growth inhibiting substance.

• Korean journal of applied entomology
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• v.36 no.3
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• pp.243-248
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• 1997

The local distribution and life cycle of Mahasma aurea (Butler) attacking Ginkgo biloba L. in Seoul and Incheon areas were studied during 1994 - 1996. The species had one generation a year. The overwintered 3rd larvae begin to feed on buds of the host plant from mid May and then on the leaves until early June. Newly hatched larvae mostly infested the leaves from mid August to late September. Pupation took place from mid June to early July, and the moths emerged in early July.

• Journal of Environmental Health Sciences
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• v.32 no.5 s.92
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• pp.492-498
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• 2006

Ultraviolet(UV) radiation causes a variety of biological effects on the skin, including inflammation, pigmentation, photoaging and cancer. Free radicals are involved in inflammatory skin reactions induced by UVB radiation. In this study, we investigated the effects of antioxidants(Tea, Korean red ginseng, Ginkgo biloba extract) on UVB-induced skin damage. Tea, KRG and EGb 761 were topically treated to dorsal skin of ICR mouse. The mice were also treated soon after IMED ($1.4KJ/m^{2}$) of UVB irradiation. Skin pigmentation of irradiated mouse was observed by a chromameter after 2 weeks. Topical application of Tea, KRG and EGb 761 for 2 weeks decreased skin pigmentation compared to DVB control group(p<.05). Tea, KRG and EGb 761 also reduced UVB-induced infiltration of inflammatory cells. These results showed that Tea, KRG and EGb 761 as a topical application may have preventive effect against UVB-induced skin damage.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.11 no.1
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• pp.240-250
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• 1998

Background: Ginkgo biloba extract is used in disorser of cerebral and peripheral blood circulation, dysfunction of brain, atherosclerosis etc., but there are little study about GbE in oriental medicine. We wished to assessthe efficacy of GbE for the treatment of cerebral infarction Method : The study group comprised 40 patients who arrived at hospital during 48 hours after attack. All patient were devided into two group. The control group was treated with Uhuangcheongsimhuan, Seonghyangjeonggisan, acupuncture therapy only, while the GbE group was treated with above therapy plus 5 days of administration of GbE(40mg three times per day). Result: 1. Symptom improve scores did not showed significant difference between control and GbE group. 2. Vasoreactivity of carotid siphon increased significantly in GbE group after treatment (in the left only : p<0.05). 3. Vasorcactivity of radial artery increased significantly in GbE group after treatment(in the right only ; p<0.05). 4. PT, a-PTT, Fibrinogen did not showed significant changes between before and after treatment in both group. Conclusion: These findings suggest that vasoreactivity increasing effect of GbE may be useful in the prevention and treatment of cerebral infarction. But the vasoreactivity increasing effect of GbE may be different from symptom imroving.

• BMB Reports
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• v.39 no.2
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• pp.158-166
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• 2006

In many organisms, trehalose acts as protective metabolite against harsh environmental stresses, such as freezing, drought, nutrient starvation, heat and salt. Herein a cDNA (designated as GbTPS, GenBank Accession Number AY884150) encoding a trehalose-6-phosphate synthase homologue was isolated and characterized from the living fossil plant, Ginkgo biloba, which is highly tolerant to drought and cold. GbTPS encoded an 868-amino-acid polypeptide with a predicted isoelectric point of 5.83 and molecular mass of 97.9 kD. Amino acid sequence alignment revealed that GbTPS shared high identity with class II trehalose-6-phosphate synthase homologues (67% identical to AtTPS7), but had only 17% and 23% of identity with OstA from Escherichia coli and ScTPS1 from S. cerevisiae, respectively. DNA gel blot analysis indicated that GbTPS belonged to a small multi-gene family. The expression analysis by RT-PCR showed that GbTPS expressed in a tissue-specific manner in G biloba and might involve in leaf development. GbTPS was also found to be induced by a variety of stresses including cold, salt, drought and mannitol.

• Molecules and Cells
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• v.26 no.6
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• pp.536-547
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• 2008

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzed the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. The full-length cDNA and genomic DNA sequences of ANS gene (designated as GbANS) were isolated from Ginkgo biloba for the first time. The full-length cDNA of GbANS contained a 1062-bp open reading frame (ORF) encoding a 354-amino-acid protein. The genomic DNA analysis showed that GbANS gene had three exons and two introns. The deduced GbANS protein showed high identities to other plant ANSs. The conserved amino acids (H-X-D) ligating ferrous iron and residues (R-X-S) participating in 2-oxoglutarate binding were found in GbANS at the similar positions like other ANSs. Southern blot analysis indicated that GbANS belonged to a multi-gene family. The expression analysis by real-time PCR showed that GbANS expressed in a tissue-specific manner in G. biloba. GbANS was also found to be up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS. The recombinant protein was successfully expressed in E. coli strain with pET-28a vector. The in vitro enzyme activity assay by HPLC indicated that recombinant GbANS protein could catalyze the formation the cyanidin from leucocyanidin and conversion of dihydroquercetin to quercetin, suggesting GbANS is a bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.29 no.2
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• pp.95-102
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• 2023

Despite the easing of social distancing, demand for non-face-to-face services continues to rise. Recently, the EU is pursuing a comprehensive plastic use reduction by expanding the scope of plastic use regulations for packaging plastics according to the New Cyclical Economy Action Plan(NCEAP). In response to this trend, the packaging industry is moving away from conventional non-degradable/petroleum-based plastics and conducting research on packaging materials using biodegradable plastics such as PLA(Poly Lactic Acid), PBAT(Poly Butylene Adipate-co-butylene Terephthalate). On the other hand, ginkgo leaves occur in large quantities in Korea and act as a cause of slip accidents and flooding. In this study, a method to utilize ginkgo biloba leaf as a new alternative biomass resource was proposed by producing lactic acid through pretreatment, enzymatic saccharification, and fermentation processes. For the efficiency of lactic acid production, a comparative analysis of lignin content from before and after browning was performed. In addition, the degree of glucan extraction was evaluated by applying a pretreatment method using three catalysts: hot water, sulfuric acid, and sodium hydroxide. It is difficult to expect high production of lactic acid with single process. Therefore, an integrated process operation using both the pretreated hydrolyzate and the residual solid enzymatic saccharification solution must necessarily be applied.

• Journal of Korean Society of Forest Science
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• v.84 no.2
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• pp.131-144
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• 1995

To determine the structure of chromosome and to identify the sex chromosome of Ginkgo biloba and G. biloba var. fastigiata, the samples were obtained from root tips of trees growing in seven different provinences. The results are as follows. The basic number of somatic chromosomes was 2n=24. The range of a relative length of long chromosome was between $14.88{\sim}11.18{\mu}m$ and that of short chromosome was $8.11{\sim}6.24{\mu}m$. The chromosome sets were composed with one long pairs of m type and 11 short pairs of sm or st type. These short pairs showed the continuous descending in length. There was a satellite on the short arm of the Longest chromosome pair, and were satellites of the one or both long arm of 7th or 8th chromosome pair which were sm or st type, or the shortest st type chromosome pair. Sometimes, a satellite on the short arm of the longest chromosome pairs of Ginkgo biloba was double satellite, but that of G. biloba var. fastigiata was not. Karyotype was $2n=24=2^{2s}A^m+2B^{st\;or\;sm}+2C^{st}+2D^{st}+2E^{st}+2F^{st\;or\;sm}+2G^{sm}+2^{2s}H^{sm}\;or\;(^{1s}H^{sm}+H^{sm})+2I^{st}+2J^{st}+2K^{st}+2^{2s}L^{st}\;or\;(^{1s}L^{st}+L^{st})$. The male and female trees were not apparently distinguished by the chromsome structures. However the differences between the satellites could be used to identify the male and females. The male tree has double satellite on short arm of a longest chromosome pairs and females' has not. Also female trees have a satellite on a short chromosome more frequently than male trees.