• Food Science and Biotechnology
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• 제16권4호
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• pp.655-658
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• 2007

Bacillus polyfermenticus SCD was transformed by the recombinant shuttle vector for Bacillus and Escherichia coli containing 3 antibiotic resistant genes and an ${\alpha}$-amylase gene from Bifidobacterium adolescentis Int57. The ${\alpha}$-amylase gene fused to a secretion sequences was expressed under the control of the promoter of amylase gene from B. subtilis var. natto. The recombinant plasmid was maintained stably in the transformants producing the ${\alpha}$-amylase. The enzyme was secreted to outside of the cell and showed the similar enzyme activity as that of Bacillus subtilis BD170 under the same conditions of pH and growth temperature. Because of the relatively easy transformation and the secretion of the enzyme, the transformants of B. polyfermenticus SCD may give a new strategy in the production of foreign genes.

• 생명과학회지
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• 제24권1호
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• pp.81-85
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• 2014

산업폐수로부터 분리한 Pseudomonas sp. BCNU 106은 다양한 유기용매를 이용하여 인돌로부터 인디고를 합성할 수 있다. BCNU 106은 높은 수준의 인돌과 다량의 p-xylene, propylbenzene 그리고 mesitylene의 존재 하에서 효과적으로 인디고를 생산했다. 본 연구에서 인디고의 최대수율 조건은 20% (v/w)의 p-xylene과 4 g/l의 인돌인 것으로 확인되었다. 이러한 조건에서 인디고의 수율과 인돌의 전환효율은 각각 315.5 mg/l와 97%로 조사되었다. 그 결과 Pseudomonas sp. BCNU 106은 산업적으로 중요한 인디고 생산을 위한 잠재적인 균주가 될 것으로 판단된다.

• 천문학회보
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• 제36권2호
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• pp.145.1-145.1
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• 2011

We present results of two-color VR photometry of the intermediate polar RXS J1803. The data were aquired using the Korean 1-m telescope located at Mt. Lemmon, USA. Different "high" and "low" luminosity states, similar to other intermediate polars, were discovered. No statistically significant variability of the color index with varying luminosity was detected. The orbital variability was found to be not statistically significant. Spin maxima timings were determined, as well as the photometric ephemeris for the time interval of our observations. The spin period variations, caused by interaction of the accretion structure with the rotating magnetic white dwarf, were also detected. These variations are of complicated character, and their study requires further observations. We determine the color transformation coefficients for our photometric systems, and improve on the secondary photometric standards.

• Transactions on Electrical and Electronic Materials
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• 제3권4호
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• pp.21-26
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• 2002

Color correction methods of chromaticity coordinates using Color Matching Function (CMF) were studied to develop a device-independent portable-type urine analyzer. The reflection spectra were measured for the degrees of 10 test items of the urine reagent strip (urine strip) to develop a portable-type urine analyzer. A computer simulation was performed to quantitatively distinguish the color reactions of the urine system, by using the spectral power distribution of Light Emitting Diode(LED), the reflection of a urine strip, and spectral sensitivity of a photodiode. To develop a device-independent system, chromaticity coordinates were modified to reduce the color deviations in the urine strip, by using the temperature compensation of LED and the color transformation by CMF. The experimental values obtained by developed urine system exhibited the accuracy above 95% for all color samples.

• 건축역사연구
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• 제13권4호
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• pp.125-138
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• 2004

Open-rectangular plan Hanok is one of the major types that compose the urban tissues of Bukchon, a typical traditional residential area in Seoul. Through the comparison of the cadastral map of 1912 and the aerial photograph of 1962, the paper has given the outline of the and the distribution of Open-rectangular plan Hanok at Bukchon. And the paper defines the characteristics of Open-rectangular plan Hanok at Bukchon as followings. First, Open-rectangular plan Hanok at Bukchon is the mutated type of Open-rectangular plan Hanok in Kyong-gi Province. Second, the composition and characteristics of Bakat-chae and outer-courtyard have been changed through the adaptation itself to the compact lots of urban neighborhood. Third, the composition and characteristics of An-chae has not been changed except the inner corner bay, that gives the lights and view to An-bang through the window. And the comparison and observation of four examples, the paper defines the identities of Open-rectangular plan Hanok at Bukchon. The partial transformation comes from the conflicts and adjustments with the structure of alley and the topographical condition of lots.

• 조명전기설비학회논문지
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• 제29권11호
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• pp.66-73
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• 2015

AC-based power systems, having the advantages that voltage transformation and long distance transmission are easy, have been constructed since the last 19th century. However, DC-based power system is paid attention these days because of the development of power electronic devices as well as the increase of digital loads and distributed generation. For instance, the transmission systems using High Voltage DC (HVDC) are commercially operated in the world and the researches on distribution system using Low Voltage DC (LVDC) are gradually increased. This paper analyzes voltage sag, resulted from faults, in LVDC distribution system according to the number of poles. Modeling and simulation with various conditions are conducted by using ElectroMagnetic Transients Program (EMTP). Moreover, some countermeasures to reduce voltage sag in LVDC distribution system are suggested briefly.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.556-561
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• 2010

Lactoferrin is an 80-kDa iron-binding glycoprotein that is found in high concentrations in human milk. Human lactoferrin (hLF) has several beneficial biological activities including immune system modulation and antimicrobial activity. In the present study, we devolved a method of hLF expression through introducing the hLF gene construct into Oriza sativa cv. Nakdong using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the rice glutelin promoter was detected in the seeds of transgenic rice plants. Transformed rice plants were selected on media containing herbicide(DL-phosphinothricin) and the integration of hLF cDNA was confirmed by Southern blot analysis. The expression of the full length hLF protein from the grains of transgenic rice plants was verified by Western blot analysis. The lactoferrin expression levels in the transformed rice grains determined by enzyme-linked immunosorbant assay accounted for approximately 1.5% of total soluble protein. Taken together, these data indicate that rice grains expressing hLF can be directly incorporated into infant formula and baby food.

• 대한금속재료학회지
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• 제50권6호
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• pp.419-425
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• 2012

The purpose of this research was to develop a low Ni austenitic cast iron through replacing Ni by Cu and Mn because they are cheaper than Ni. The effect of the Cu content (6-12 wt%) on the microstructure characteristics was investigated in Fe-3%C-16%(Ni+Cu+Mn) cast iron. Contrary to general effect of the Cu on cast iron, the result of the microstructure analysis indicated that bainite and cementite were formed in high Cu content (>8 wt%Cu). A crystallized Cu-solution (Cu-Mn) phase and MnS in the Cu-solution were formed. The quantity of those phases increased as the Cu content increased. Consequently, the high Cu content in the composition ratio (Ni+Cu+Mn=16%) caused the formation of Cu-Mn/MnS and those phases decreased the effect of Cu and Mn on austenite formation. For this reason, bainite and cementite were formed in high Cu content.

• Genomics & Informatics
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• 제20권1호
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• pp.7.1-7.13
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• 2022

2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.

• 한국측량학회지
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• 제25권2호
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• pp.117-131
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• 2007

최근 지형공간정보의 활용분야가 증가하면서 건축물 내부 공간구축에 있어서 더욱 현실화된 다양한 정보의 필요성이 증대되고 있으며, 건축물 대장 및 설계도면은 효과적인 건축물의 공간 모델링에 활용되고 있다. 건축물의 실감적 3차원 시각화는 컴퓨터 그래픽 분야에 속하는 기술이지만, 시각화와 더불어 건축물의 외부 및 내부에 대한 정보생성과 공간분석을 위해서는 GIS의 다양한 기능이 요구된다. GIS에서 건축물 내부의 3차원 시각화 및 공간분석을 위해서 데이터베이스(database) 구축과정에 많은 작업이 요구되며, 건축물 설계 CAD 도면의 레이어(layer)를 편집하는 등의 전처리 과정과 CAD 도면의 좌표를 지상좌표로 변환하는 georeferencing 과정이 필요하다. 본 연구에서는 건축물 내부공간을 상세하게 구축하기 위하여 CAD 도면을 이용하였고, CAD 도면을 효율적으로 편집하기 위하여 AutoCAD VBA를 이용하여 자동화를 추구하였다. 또한, GIS의 주요기능인 공간분석을 위하여 topology를 생성하였으며, CAD 도면상의 건축물을 지상좌표로 변환하기 위하여 georeferencing을 수행하였다. 3차원 건축물 데이터베이스 구축공정을 제시하고, Georferencing 결과의 정확도를 분석하기 위하여 수치지도와 GPS 측량 자료를 이용하였다. 이와 같이 구축된 건축물 모델을 이용하여 3차원 시각화 모델을 생성하였으며, 수치지도를 기준으로 georeferencing 결과를 이용하여 건축물 내부와 건축물 간의 이동경로 설정을 위한 네트워크 모델을 생성하였다.