• Korean Journal of Organic Agriculture
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• v.13 no.3
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• pp.291-300
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• 2005

Diseases were surveyed in 5 Rice paddy field areas of Organic paddy field and conventional paddy field. The 3 major diseases, rice leaf blast, bacterial leaf blight and sheath blight in rice were surveyed at duck raising, rice bran and conventional rice paddy field. They were serious in duck raising paddy field, rice bran paddy field more than conventional paddy field. The Ilpum variety were infected seriously more than Chucheong. At the effectivity test of the environment-friendly substance for the rice-seed sterilization, 1000-times diluted brown-rice vinegar showed controlling effect against Bakanae disease, and germination rate also was good. There was no Weeds Control efficacy by cultivating of hairy vetch in rice paddy field. However, extract of hairy vetch Leaf and root repressed the germinating of lettuce seed.

• Journal of Korean Society of Forest Science
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• v.28 no.1
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• pp.36-42
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• 1975

In the beginning of the germination of Castanea creata seeds, seedlings with identical genetic characteristics from only one seed were produced by longitudinally splitting the radicle and epicotyl in half with a razor blade. The results are summarized as follows: 1. 2-8 genetically identical Castanca crenata seedlings were produced by longitudinally splitting the radicles and epicotyles from one to four in number in half with a razor blade 1-3 times about 7 days apart, while the seeds were germinating. 2. The proportion of success of splitting seedlings was very high (90-100%). When some of the spliting seedlings were withered a sprout appeared again and it was possible the seedling to revive. 3. The production rate, growth and T/R rate of seedlings which were split only once were lower that those of the seedlings which were split several times. 4. The growth and T/R rate of split seedlings which were one of no more than four seedlings produced from one seed showed no difference in comparison with normal seedlings, but in seedlings which were one of more than four, the growth and T/R rate were considerably decreased. 5. With the progress of growth, the external shapes of splitting seedlings completely returned to the original state, and no problems in growth, were found.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.4
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• pp.64-73
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• 1999

This study was conducted to find out the effects of hydroseeding material and seed mixture on the revegetation of rock cut-slopes by PEC4 (Polymer-Ecology-Control) Hydroseeding Measures. PEC4 hydroseeding material was applied to four cut-slopes using hydroseeding measures from April to August, 1999, and the field survey was carried out by monthly. PEC4 material consisted of bark compost and organic soil amendments. This material has high content of organic matter and high level of water holding capacity. PEC4 hydroseeding material shows low level of soil hardness, so it gives to good condition for seed germinating and plant growing in early stage. PEC4 material attached at rock cut-slopes by two types of adhesive agent was not eroded by rainfall. The plant coverage and number of plant species were affected by mixing ratio of seeds and seeding timing. From the viewpoint of plant establishment, the optimal hydroseeding timing of mixed seeds for plant growth seems to be in May. Most of the plant seeds were germinated well and they covered rock cut-slopes so quickly and effectively. Plant importance value of Silene armeria and Platycodon grandiflorum. were higher than any other seeded-native species in the competition between native species and exotic species, so they have enough possibility to be used for slope revegetation works. Thus it leads to conclusion that the revegetation method used in this experiment was a very effective method for plant establishment on rock cut-slopes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.53-62
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• 1985

A radioimmunoassay technique has been developed for the determination of abscisic acid (ABA) in crude extracts from germinating rice (Oryza sativa L.). By this method, the changes in ABA level of rice during germination was investigated. The ABA content in rice seeds was found to be 76.5ng/g dry weight in Dong-jin variety and 91.1ng/g dry weight in Sam-gang variety. A rapid decrease in ABA content of rice occurred during germination within 24 hours after seed imbition. The decreasing rate of ABA content during germination showed a significant direct proportion to the imbibition temperature and water-absorbing rate of rice. The decrease in ABA content during germination was found to be caused partly by an elution of ABA from the tissue to the imbibing fluid, and partly by a metabolic conversion of ABA to another compounds. The germination process of rice occurred only when the tissue ABA level decreased below a certain level, and the decreasing rate of ABA level during germination correlated with the ability for germination at low temperature of rice.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.72-82
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• 2009

Research was initiated to investigate early establislment characteristics and germination pattern of perennial ryegrass (PR, Lolium perenne L.). Eight varieties of PR were evaluated in the study. An alternative environmental condition for a PR germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination pattern were measured and analyzed daily for 30 days. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and final germination rate. Germination rates were variable among the varieties, being 66.00 to 93.75% at the end of study. There were considerable variations in early germination characteristics and germination pattern among PR varieties. The first germination in all entries tested was initiated from 3 to 5 DAS (days after seeding) under ISTA conditions, being 2 days in differences among the varieties. It was 4 to 7 DAS in days to the 50% germination, being 1 to 3 days later from the first germination and three days were variable among them. Days to the 75% germination were 5.93 to 10.50 DAS under ISTA conditions, being 4.57 days in variety differences. Pennant II and Brightstar II were the fastest varieties. The slowest ones were Revenge GLX and Brightstar SLT. Considering days to the first germination, days to 75% germination, and germination pattern etc, Pennant II and Brightstar II were regarded as excellent varieties under ISTA conditions. From tills study, information on differences in germination characteristics and pattern would be usefully applied for golf course design and construction, when established with PR.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.5
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• pp.81-89
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• 2010

This study was conducted to evaluate the effects of early revegetation by analyzing the characteristics of germination and growth of Chrysanthemum zawadskii using vegetation-plant in rock slopes. After making up a growing basis of approximately 20-cm depth and 10-cm diameter by using a boring machine, the surface of rock slopes was planted with vegetation-plant. The number of germinating populations by soil media was 41 in H.s, 4 in T.s, 3 in M.s, and 0 in M.g.s. The germination rate (%) by soil media was 20.0% in H.s, 3.3% in T.s, 2.5% in M.s and 0% in M.g.s. In monthly changes of growth rate, the aspect was northwest direction, the soil media was H.s, and the treatment was microorganism plot. The main factors affecting survivorship and growth of population were soil media and treatment plot. The interaction between each factor had a good effects in bearing x treatment plot, soil media x treatment plot. but, it is recommended that the mulching of vegetation plant is highly needed to help the germination of seed and growth of vegetation because of loss of seed and soil media occurred due to rainfall. Therefore, The result suggests that the revegetation technique using boring in rock slope was very efficient in respect of the early revegetation and the landscape.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.3
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• pp.607-612
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• 2009

For the development of ecological sound proof wall, successful seed germination and growth of plants are very important. In our present study, we observed germination of thirty five different seed species in growth chamber at $24^{\circ}C$, humidity 60% and alternation of 12 hours photoperiods. Germination of same seeds were carried out at room temperature on 6:4, 7:3, 8:2, and 9:1 proportion of pure and mineral soils respectively. Out of studied species, maximum germination was recorded for Perennial penant (92%), followed by Brassica napus (86%), and Silene armeria (82%). The measurement of root/shoot length showed, maximum root/shoot length for Perennial penant, followed by perennial sonata and Rebolsentri sp. Based on germination percentage and root/shoot length of germinating seeds, we considered Perennial penant, perennial sonata and Rebolsentri sp. were proper species for the construction of ecological sound proof walls

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.159-168
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• 1998

Effect of plant growth regulators and end-product on the enzyme activities in cotyledons of Vigna angularis during germination was investigated by measuring the changes of $\alpha-amylase$ activities in attached and detached cotyledons applied growth regulators and sugars. The higher levels of $\alpha-amylase$ in detached cotyledons than those in cotyledons attached to the embryonic axis were due to both faster synthesis and slower degradation of the enzyme in the detached cotyledons than in the attached cotyledons. Levels of $\alpha-amylase$ activity were reduced by high concentrations of glucose and sucrose, and it is suggested that this effect was caused mostly by osmotic stress and partly by end-product repression. In detached cotyledons exogenously supplied $GA_3,$ IAA, kinetin, or their combinations has a small promotive effect on the developmental patterns of $\alpha-amylase$ activity ABA and uniconazole both prevented the synthesis of $\alpha-amylase$. Glucose inhibition of enzyme activity was partly reversed by the application of $GA_3,$ and CAMP. $GA_3,$ and cAMP seemed to act through a similar mechanism. The addition of inhibitors of protein and RNA synthesis largely prevented the increase of enzyme activity in the presence or absence of exogenous $GA_3,$. The pretreatment experiments with canavanine indicated that the earlier the time of addition was, the lower the amylase activity was.

• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.213-219
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• 2004

Zygotic embryos were excised from immature and mature seeds of the Himalayan yew, Taxus wallichiana. The embryos germinated precociously when kept in darkness for 5 weeks and developed into full seedlings within 10-12 weeks. The highest rate of embryo germination ($81\%$) was obtained in modified Lloyd & McCown' s woody plant medium containing macro and micronutrients at half strength supplemented with $1\%$ activated charcoal, which supported both the best embryonic growth ($43\%$) and seedling development ($32\%$). However, the supplementation of basal media with kinetin, thidiazuron, 6-benzyl aminopurine or $GA_3$ had no effect on the germination of the embryos. The embryos derived from immature seeds germinated but the frequency of embryonic growth was better in mature seeds. Stratification of seeds effected precocious germination of embryos. Seeds kept at $4^{\circ}C$ for 1 week germinated earlier and at a higher frequency irrespective of the stage of seed maturity, while the germination rate declined with prolonged cold treatment for 1 month at that same temperature. Analysis of taxanes in germinating seedlings revealed that root tissues contained high levels of taxol, 10-deacetyl-baccatin ill and baccatin ill as compared to shoots. Thus embryo culture technique appears to overcome the lengthy dormancy requirement of T. wallichiana seeds.

• Journal of Life Science
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• v.11 no.5
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• pp.489-495
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• 2001

The effect of ozone-water treatment on the morphological change of endosperm cells and the activity of acid phosphatase during Glycine max germination was investigated with electron microscope. Acid phosphatase showed the activity in the cell organelles of germinating endosperm of seed. it's activity occurrs in 12 hrs cultivation after 0.5 ppm ozone-water treatment. As the differentiation of endosperm, reaction products of the acid phosphatase appear to be accumulated invacuole after treatment of ozone-water. This result confirm that acid phosphatase is inveolved in the decomposition and translation of the intracellular storage materials. The characteristics of grganelle in the endosperm cell during germination were discussed.