• 제목/요약/키워드: gentamicin resistance

검색결과 212건 처리시간 0.02초

서해안 수산생물에서 분리한 대장균(Escherichia coli)의 항생제 내성 및 다제 내성 양상 비교 (Comparison of Antimicrobial Resistance and Multi-Drug Resistance Patterns of Escherichia coli Isolated from Aquatic Organisms Off the West Coast of South Korea)

  • 정연겸;박보미;김민주;박진일;정연중;오은경
    • 한국수산과학회지
    • /
    • 제54권4호
    • /
    • pp.388-396
    • /
    • 2021
  • Antimicrobial resistance patterns of Escherichia coli were investigated. Strains were isolated from 310 shellfish, 36 crustaceans, and 12 fish collected off the West Coast of Korea from April 2019 to October 2020. Two hundred and ninety-five E. coli strains were isolated from shellfish, 100 from crustaceans, and 54 from fish. Strains isolated from shellfish showed the highest resistance to ampicillin (27.5%), whereas those from crustaceans were resistant to sulfisoxazole (30.0%) and those from fish were resistant to ampicillin (59.3%) and sulfisoxazole (59.3%). Ceftazidime resistance was observed in strains isolated from short neck and hard clams, whereas gentamicin resistance was observed in strains from fish. Multi-drug resistance was observed in 56 strains (48.7%) isolated from shellfish, 11 (28.2%) from crustaceans and 27 (73.0%) from fish. Depending on the source of isolation, the strains showed specific antimicrobial resistance tendency. Strains isolated from shellfish showed 12 different multi-drug resistance patterns, whereas those from crustaceans showed high resistance (59%) to a single antimicrobial agent and those from fish showed a broad trend of multi-drug resistance to more than eight antimicrobials.

임상검체로부터 분리된 methicillin 내성 Staphylococcus aureus의 독소 및 항생제 내성 (Toxins and Antibiotic Resistance of Methicillin-Resistant Staphylococcus aureus Isolated from Clinical Specimens)

  • 백근식;기광서;최한나;박성찬;고은초;김형락;성치남
    • 생명과학회지
    • /
    • 제21권2호
    • /
    • pp.257-264
    • /
    • 2011
  • 2009년 7월부터 12월까지 순천 소재 한 병원에 내원한 환자의 검체로부터 methicillin 내성 Staphylococcus aureus (MRSA) 75균주와 methicillin 감수성 S. aureus (MSSA) 24균주를 분리하였다. 분리균의 항생제 감수성 조사는 디스크 확산법을 사용하여 측정하였다. 분리균의 독소 유전자 보유는 multiplex PCR을 이용하여 장독소(enterotoxin; SE), 독성 쇼크 증상 독소 1(toxic shock syndrome toxin-1; TSST-1), 피부박탈성 독소(exfoliative toxin; ET) 및 백혈구 용해 독소(Panton-Valentine leukocidin; PVL) 유전자를 검출하였다. 분리된 MRSA 60개 균주는 1개 혹은 2개의 독소 유전자를 가지고 있으며, 22.7%의 균주가 seb, sec, seg, sei와 tst 유전자를 동시에 보유하고 있었으며 18.7%는 sec, seg, sei와 tst 유전자를 동시에 보유하고 있었다. 백혈구 용해독소를 암호하는 pvl 유전자는 검출되지 않았다. MRSA는 sec, seg, sei와 tst 유전자 보유에 높은 상관성을 보였다. MRSA 균주들은 erythromycin (분리균의 89%), gentamicin (70.7%), ciprofloxacin (69.3%), clindamycin (61.3%)과 tetracycline (58.7%)에 내성이 높은 반면, MSSA 균주들은 erythromycin를 제외한 다른 항생제에는 민감하였다. 독소 유전자 seb, sec와 tst는 tetracycline 내성과 높은 상관관계가 있었다.

오리 분변에서의 Campylobacter jejuni 오염도와 항생제 내성유형 조사 (Prevalence and antimicrobial resistance patterns of Campylobacter jejuni from duck feces)

  • 김능희;채희선;강영일;신방우;최농훈;김효비
    • 한국동물위생학회지
    • /
    • 제36권1호
    • /
    • pp.57-60
    • /
    • 2013
  • This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.

영남지방 돼지에서 분리한 Pasteurella multocida의 협막혈청형 및 항균제 감수성 조사 (Capsular serogrouping and antimicrobial drug susceptibility of Pasteurella multocida isolated from Youngnam swine herds)

  • 조길재;김봉환;탁연빈
    • 대한수의학회지
    • /
    • 제29권4호
    • /
    • pp.487-492
    • /
    • 1989
  • The capsular serogroupes and drug susceptibility of 111 isolates of Pasteurella multocida from pigs with atrophic rhinitis and pneumonic lesions were investigated. Of the 111 P multocida isolates, 42 were from lung lesions, 47 from nasal turbinate lesions and the remaining 22 from the nasal swabs. P multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavine auto-agglutination. Most isolates(64.9%) were type A, 23.4% were type D and the remaining 11.7% were untypable. Resistance to triple sulfa(97.3%) was most frequent, followed by resistance to tiamulin(71.2%), tylosin(56.8%), streptomycin(36.9%), and neomycin(36.0%). The majority of the organisms were susceptible in order of prevalence to baytril(100%), ampicillin(98.2%), linsmycin(97.3%), colistin(97.3%), cephalothin(94.6%), gentamicin(93.7%), amikacin(92.3%), tetracycline(91.9%), trimethoprim/sulfamethoxazole(91.0%), and kanamycin(90.1%). No differences in drug resistance in relation to capsular serogroupes of P multocida and the origin of lesions were noted. A high prevalence of multiple drug resistance was observed and the most common resistant patterns were Sss, Tm, Ty(12.6%) and Sm, Sss, Tm, Ty(8.1%) patterns.

  • PDF

돼지에서 분리한 Actinobacillus pleuropneumoniae의 혈청형 분포 및 항생제 감수성 (Serotype and antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolates from pigs in Korea)

  • 정지열;장현
    • 대한수의학회지
    • /
    • 제52권3호
    • /
    • pp.177-181
    • /
    • 2012
  • Actinobacillus (A.) pleuropneumoniae is the causative agent of pleuropneumonia which is one of the most important respiratory diseases in pigs worldwide. A total of 32 A. pleuropneumoniae isolates from diseased pigs during 2008 to 2010 were serotyped by polymerase chain reaction method. The susceptibility of the isolates to 13 antimicrobial agents were determined by disk diffusion test. In all the 32 isolates examined in this study, serotype 5 (16 isolates: 50%), 1 (7 isolates: 21.9%), 2 (5 isolates: 15.6%) and 12 (1 isolate: 3.1%) were found. Of all tested antimicrobial agents, resistance to oxytetracycline was found in 96.9% of isolates, followed by resistance to amikacin (81.2%), neomycin (68.7%), kanamycin (53.1%), penicillin (50.0%), gentamicin (43.7%), florfenicol (25.0%), ampicillin (18.7%), colistin (9.4%), trimethoprim/sulfamethoxazole, ceftiofur (8.3%), amoxicillin/clavulanic acid (3.1%) and enrofloxacin (0%). Oxytetracycline or florfenicol-resistant isolates were examined for the presence of resistance gene. Among the 31 oxytetracycline-resistant isolates, tetB, tetH and tetO genes were detected in 22 (71%), 8 (26%) and 1 (3%) isolates, respectively. The floR genes were detected in 8 (100%) of the 8 florfenicol-resistant A. pleuropneumoniae isolates.

Antimicrobial Susceptibility and Clonal Relatedness between Community- and Hospital-Acquired Methicillin-Resistant Staphylococcus aureus from Blood Cultures

  • Jung Sook-In;Shin Dong-Hyeon;Park Kyeong-Hwa;Shin Jong-Hee
    • Journal of Microbiology
    • /
    • 제44권3호
    • /
    • pp.336-343
    • /
    • 2006
  • We compared the antimicrobial resistance and clonal relationships among the community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) strains that were isolated from blood cultures in a university hospital over a 4-year period. A total of 131 MRSA isolates, including 28 CA-MRSA and 103 HA-MRSA strains, were identified; antimicrobial susceptibility testing indicated that the CA-MRSA isolates were more susceptible to erythromycin (21 % vs 6% ; P=0.02), clindamycin (46% vs 12%; P<0.01), ciprofloxacin (43% vs 11%; P<0.01), and gentamicin (43% vs 6%; P<0.01) than were the HA-MRSA isolates. Pulsed-field gel electrophoresis (PFGE) typing and antimicrobial resistance profiles separated the 20 CA-MRSA isolates into 14 and 10 different patterns, respectively, and the 53 HA-MRSA isolates were separated into 24 and 7 different patterns, respectively. Twenty-one (40%) of the 53 HA-MRSA isolates belonged to two predominant PFGE types, and most of them showed multi-drug resistant patterns. Four (20%) of the 20 CA-MRSA and 10 (19%) of the 53 HA-MRSA isolates fell into two common PFGE patterns, and each of them showed the same multi-drug resistant pattern. This study suggests that, although the CA-MRSA blood isolates showed diverse PFGE and antimicrobial resistance patterns, some of these isolates may have originated from the HA-MRSA strains.

클로람페니콜 내성 황색포도상구균의 플라스미드 동정 (Characterization of Plasmids of Chloramphenicol Resistant Staphylococcus aureus)

  • 박정희;이재윤;문경호
    • 약학회지
    • /
    • 제52권1호
    • /
    • pp.33-36
    • /
    • 2008
  • Staphylococcus aureus KH13 and Staphylococcus aureus KH28 were resistant to chloramphenicol, ampicillin, clindamycin, erythromycin, gentamicin, kanamycin, streptomycin, tobramycin, and norfloxacin. A plasmid (pKH13) and two plasmids (pKH14, pKH15) were isolated from Staphylococcus aureus KH13 and Staphylococcus aureus KH28, respectively and complete nucleotide sequences of three plasmids were determined. It was found that pKH13 and pKH15 mediated chloramphenicol resistance and pKH14 was a cryptic plasmid.

다제내성 황색포도상구균이 가지고 있는 클로람페니콜 내성 플라스미드의 동정 (Characterization of Chloramphenicol Resistant Plasmid of Multidrug-resistant Staphylococcus aureus)

  • 이대운;문경호
    • 약학회지
    • /
    • 제37권6호
    • /
    • pp.621-624
    • /
    • 1993
  • The clirical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEll, Hindlll, Hpall, and Xbal. The above restriction endonucleases have a single site, but nucleases BamHl, Bgll, BglII, EcoRl, EcoRV, HaeIII, Hpal, Kpnl, Pstl, PvnII, Sall, Smal, and XhoI have no site on this plasmid.

  • PDF

동물에서 분리한 Proteus mirabilis의 항생제 감수성에 관한 연구 (Antibiotics susceptibility of Proteus mirabilis isolated from domestic animals in Chonbuk province)

  • 조정곤
    • 한국동물위생학회지
    • /
    • 제26권2호
    • /
    • pp.95-103
    • /
    • 2003
  • Isolates of 70 Proteus mirabilis were tested against 10 different antibiotics by a disc diffusion method as recommended by National Committee for Clinical Laboratory Standards (NCCLS). The isolates were resistant in order of tetracycline(100.0%), enrofloxacin(95.7%), ampicillin(74.3%), choramphenicol(62.9%), cephalothin(58.6%), streptomycin(50%), kanamycin(47.2%), neomycin(35.8%), gentamicin(15.7%), and amikacin(2.9%). In the antibiotic resistant patterns, 37 kinds of multiple resistance patterns of P mirabilis isolates were detected. The highest resistant pattern was ampicillin-cephalothin-chloramphenicol-enrofloxacin-tetracycline(11.6%).

동물에서 분리된 용혈성 대장균의 항생제 내성 (Antibiotic Resistance of Hemolytic Escherichia coli Isolated from Animals in Korea)

  • 이계남;박용호;정병열;이연희
    • 미생물학회지
    • /
    • 제39권4호
    • /
    • pp.293-295
    • /
    • 2003
  • Total 70 isolates of Escherichia coli obtained from pigs were studied. Forty four isolates had $\textregistered$-hemolytic activity which was heat labile. Minimum inhibitory concentration test indicated that 40 isolates (57.1%), 15 isolates (21.4%), 23 isolates (32.9%), and 5 isolates (7.1%) were resistant to ampicillin, cephalothin, gentamicin, and norfloxacin, respectively. None of them were extended spectrum $\textregistered$-lactamases (ESBLs) producer when the double disk synergy test (DDST) was performed.