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http://dx.doi.org/10.5352/JLS.2011.21.2.257

Toxins and Antibiotic Resistance of Methicillin-Resistant Staphylococcus aureus Isolated from Clinical Specimens  

Baik, Keun-Sik (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Ki, Gwang-Seo (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Choe, Han-Na (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Park, Seong-Chan (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Koh, Eun-Cho (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Kim, Hyung-Rak (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Seong, Chi-Nam (Department of Biology, College of Life Science and Natural Resources, Sunchon National University)
Publication Information
Journal of Life Science / v.21, no.2, 2011 , pp. 257-264 More about this Journal
Abstract
Seventy five methicillin- resistant Staphylococcus aureus (MRSA) strains and 24 methicillin- susceptible S. aureus (MSSA) were isolated from clinical specimens obtained from a hospital in Suncheon, Jeonnam province, Korea, from July to December, 2009. Antibiotic resistance was determined using the disc diffusion method. Genes encoding enterotoxin (SE), toxic shock syndrome toxin-1 (TSST-1), exfoliative toxin (ET) and Panton-Valentine leukocidin (PVL) were detected by multiplex PCR-mediated amplification using specific primers. Sixty (80%) MRSA isolates possessed either one or more toxin genes and the most common pattern that coexisted in MRSA was seb, sec, seg, sei and tst (22.7%) followed by coexistence of sec, seg, sei and tst genes (18.7%). Gene pvl encoding leukocidin was not found. Significant correlation between the production of sec, seg, sei and tst genes was found. MRSAs were resistant to erythromycin (89% of the isolates), gentamicin (70.7%), ciprofloxacin (69.3%), clindamycin (61.3%) and tetracycline (58.7%), while MSSAs were susceptible to the antibiotics with the exception of erythromycin. Toxin genes seb, sec and tst were related to the tetracycline resistance of MRSA.
Keywords
Methicillin- resistant Staphylococcus aureus (MRSA); antibiotic resistance; enterotoxin; tetracycline resistance;
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