• Title/Summary/Keyword: genotypes

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Responses of Soybean Genotypes to Different Levels of Irrigation

  • Rabbani, M.F.;Ashrafuzzaman, M.;Hoque, A.M.;Karim, M.Abdul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.2
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    • pp.131-135
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    • 2004
  • To find out the responses of soybean genotypes in terms of different levels of irrigation with the aim of evaluating the growth, yield, and its optimum levels of irrigation, an experiment was conducted at the Field of Crop Botany Department, Bangladesh Agricultural University, Mymensingh during the period from November 2000 to February 2001. Five levels of irrigation viz. $\textrm{I}_0$: no irrigation, $\textrm{I}_1$: one time irrigation at 20 days after sowing (DAS), $\textrm{I}_2$:two times irrigation at 20 and 40 DAS, $\textrm{I}_3$: three times irrigation at 20, 40, and 60 DAS, and $\textrm{I}_4$: four times irrigation at 20, 40, 60, and 80 DAS and three genotypes of soybean viz. BS-3, BS-16, and BS-60 were used in this experiment. The crop was grown in a split plot design having three replications. The plant height, leaf area index, crop growth rate, shoot dry weight, branches $\textrm{plantI}_{-1}$, filled pods $\textrm{plantI}_{-1}$, seeds $\textrm{plantI}_{-1}$, seed yield, and harvest index were influenced significantly by irrigation and these were found to be highest at three times irrigation except branches $\textrm{plantI}_{-1}$. The chlorophyll content increased but empty pods $\textrm{plantI}_{-1}$ decreased with increase in irrigation levels. Genotypes of soybean varied significantly in terms of growth attributes at various growth stages except shoot dry weight at 90 DAS. The genotype BS-3 performed better compared to other genotypes and gave maximum seed yield.

Response of Barley Genotypes to Fusarium Head Blight under Natural Infection and Artificial Inoculation Conditions

  • Khanal, Raja;Choo, Thin Meiw;Xue, Allen G.;Vigier, Bernard;Savard, Marc E.;Blackwell, Barbara;Wang, Junmei;Yang, Jianming;Martin, Richard A.
    • The Plant Pathology Journal
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    • v.37 no.5
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    • pp.455-464
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    • 2021
  • Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of Fusarium graminearum by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of F. graminearum by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (r = 0.47, P < 0.01) and FHB incidence (r = 0.56, P < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (r = 0.12, P > 0.05) under natural infection and it was not correlated with DON concentration (r = -0.23, P > 0.05) and FHB incidence (r = 0.19, P > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.

The whole wheat effect and refined with E-selectin polymorphism on breast cancer

  • Zakariya, Bilal Fadil;Almohaidi, Asmaa M. Salih;Simsek, Secil Akilli;Kamal, Areege Mustafa;Al-Dabbagh, Wijdan H.;Al-Waysi, Safaa A.
    • Analytical Science and Technology
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    • v.35 no.4
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    • pp.161-168
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    • 2022
  • Wheat is rich in sources of fiber, oligosaccharides, and resistant starch, simple carbohydrates which may have a protective role against carcinoma. Additionally, Whole wheat/bran as well includes contains phytochemicals such as flavonoids, lignans, folate, phytosterols, phenolic acids, and tocols. The above phytochemicals suitable forms antioxidant and cholesterol-reducing activities. Phytoestrogens are regarded as especially essential in the preventative measures of hormonally dependent malignancies such as breast cancer (BC). In this study lowered BC risk has been associated with whole grain/bran consumption with an odds ratio (OR=0.24 and 95 %CI=0.10-0.56). Wheat/bran appears to have a reliable protective impact against BC. While intake of white bread has been associated with a high risk of BC (OR=2.63 and CI 95 %=1.07-6.48). Also, the E-Selectin (SELE) Single nucleotide polymorphisms (SNPs) rs5353 A/G and rs932307 C/T were investigated using the sanger sequences approach. There was a positive association between genotypes (rs5353 GG+AG) and (rs932307 TT+CT) with rate consumption of wheat-white/bread and these genotypes were more frequent in patients had BC in comparison with a significant difference (P=0.03) (P=0.01), respectively. The genotypes (GG+AG) frequency of the rs5353 polymorphism and (TT+CT) genotypes of the rs932307 polymorphism in the present study had a high risk of cancer with (OR=3.05), (OR=4.17) respectively. While these genotypes showed no significant association with the rate of whole grain consumption in patients and control. Therefore, the type of wheat may associate with increased incidence of disease-related with type of Polymorphism because some present genotypes of SNPs showed high (OR) which may refer to their positive associated with disease, the white wheat consumption may active the risky association between SNPs and BC.

Association analysis of polymorphisms in six keratin genes with wool traits in sheep

  • Sulayman, Ablat;Tursun, Mahira;Sulaiman, Yiming;Huang, Xixia;Tian, Kechuan;Tian, Yuezhen;Xu, Xinming;Fu, Xuefeng;Mamat, Amat;Tulafu, Hanikezi
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.6
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    • pp.775-783
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    • 2018
  • Objective: The purpose of this study was to investigate the genetic effects of six keratin (KRT) genes on the wool traits of 418 Chinese Merino (Xinjiang type) (CMXT) individuals. Methods: To explore the effects and association of six KRT genes on sheep wool traits, The polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP), DNA sequencing, and the gene pyramiding effect methods were used. Results: We report 20 mutation sites (single-nucleotide polymorphisms) within the six KRT genes, in which twelve induced silent mutations; five induced missense mutations and resulted in $Ile{\rightarrow}Thr$, $Glu{\rightarrow}Asp$, $Gly{\rightarrow}Ala$, $Ala{\rightarrow}Ser$, $Se{\rightarrow}His$; two were nonsense mutations and one was a same-sense mutation. Association analysis showed that two genotypes of the KRT31 gene were significantly associated with fiber diameter (p<0.05); three genotypes of the KRT36 gene were significantly associated with wool fineness score and fiber diameter (p<0.05), three genotypes of the KRT38 gene were significantly associated with the number of crimps (p<0.05); and three genotypes of the KRT85 gene were significantly associated with wool crimps score, body size, and fiber diameter (p<0.05). Analysis of the gene pyramiding effect between the different genotypes of the gene loci KRT36, KRT38, and KRT85, each genotype in a gene locus was combined with all the genotypes of another two gene loci and formed the different three loci combinations, indicated a total of 26 types of possible combined genotypes in the analyzed population. Compared with the other combined genotypes, the combinations CC-GG-II, CC-HH-IJ, CC-HH-JJ, DD-HH-JJ, CC-GH-IJ, and CC-GH-JJ at gene loci KRT36, KRT38, and KRT85, respectively, had a greater effect on wool traits (p<0.05). Conclusion: Our results indicate that the mutation loci of KRT31, KRT36, KRT38, and KRT85 genes, as well as the combinations at gene loci KRT36, KRT38, and KRT85 in CMXT have significant effects on wool traits, suggesting that these genes are important candidate genes for wool traits, which will contribute to sheep breeding and provide a molecular basis for improved wool quality in sheep.

Changes in Physiological Characteristics of Barley Genotypes under Drought Stress (한발저항성 정도가 다른 보리 품종들의 한발처리에 따른 생리적 특성변화)

  • 이변우;부금동;백남천;김정곤
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.506-515
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    • 2003
  • Six barley varieties that showed different degree of drought tolerance were grown with and without drought stress treatment (control), and investigated for the temporal changes in growth and several physiological traits after drought treatment. Soil water potential was -0.05 ㎫ at the initial stage of drought treatment and dropped to -0.29 ㎫ at 19 days after withholding irrigation. Soil water potential (SWP) maintained at -0.05 ㎫ in the control. The dry weight (DW) under the drought treatment were reduced compared to the control as follows: Dicktoo-S (short awn), 69% ; Dicktoo-L (long awn), 70%; Dicktoo-T (tetra), 86%; Dongbori-1, 69%; Suwonssalbori-365, 55% and Tapgolbori, ,37%. Dicktoo lines and Dongbori-1 were more tolerant than Suwonssalbori-365 and Tapgolbori. Leaf relative water contents (RWC) and leaf water potential (LWP) decreased obviously under the drought condition, the decrease being greater especially in the less drought-tolerant barley genotypes. Dongbori-1 and Dicktoo-L in drought treatment showed net photosynthesis of 38% and 17% compared to the control, respectively, and the other four genotypes much lower photosynthesis of 1.1% to 7.0%. Stomatal conductance, mesophyll conductance, and the photochemical efficiency (Fv/Fm) of PS II were reduced by drought treatment, the reduction being greater in drought-sensitive genotypes. The drought-tolerant genotypes had greater osmotic adjustment (OA) capacity under water stress. Thus, the decrease of RWC and LWP was lower and the turgor pressure conservation capacity was higher under water stress in drought-tolerant genotypes. Drought-tolerant genotypes showed less decrease of photosynthesis because stomatal conductance, mesophyll conductance and the ratio (Fv/Fm) of the variable to maximal fluorescence of drought-resistant genotype was decreased less in the drought stress condition. In conclusion, the drought-tolerant genotypes had better water conservation capacity through efficient OA, and this led to the lower decrease of photosynthesis and growth in water stress condition.

Variation of Antioxidant Activity and Bioactive Compounds Content in Cucurbitaceas and Solanaceae Seeds (박과와 가지과 유전자원 종자의 항산화력 및 바이오 활성 화합물 함량 변이)

  • Kim, Sung Kyeom;Lee, Sang Gyu;Lee, Hee Ju;Choi, Chang Sun;Kim, Jin Sun;Kim, Su;Lee, Woo Moon
    • Journal of agriculture & life science
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    • v.51 no.2
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    • pp.47-59
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    • 2017
  • The objectives of this study were to select the seeds of Cucurbitaceae and Solanaceae genotypes in terms of superior with bioactive compounds content and to inform sophisticated data for developing the high value-added products. We evaluated to aspects of the antioxidant activity, polyphenol content, and flavonoid contents in seeds from two vegetable family. We used in the Cucurbitaceae(watermelon, squash, bitter gourd, and sponge gourd) and Solanaceae(hot pepper, sweet pepper, and egg plant) the total 408 genotypes. In Cucurbitaceae, polyphenol content of watermelon and squash genotypes were ranged 19.9-343.8 and $6.1-81.2mg{\cdot}100g^{-1}\;DW$, respectively. The polyphenol content of watermelon genotypes was 12% among all genotypes over $160mg{\cdot}100g^{-1}\;DW$. The mean of flavonoid content in watermelon and squash genotypes represented 80 and $41.3mg{\cdot}100g^{-1}\;DW$, respectively. In Solanaceae, flavonoid content of hot pepper genotypes was ranged $64.4-472.5mg{\cdot}100g^{-1}\;DW$, with an average of $165.0mg{\cdot}100g^{-1}$. The 23 hot pepper genotypes were classified over 90% antioxidant activity. The antioxidant activity of sweet pepper was ranged 35.9-90.3%, and 23% of all genotypes represented 82% antioxidant activity. The polyphenol and flavonoid content of egg plant was ranged $38.1-642.0mg{\cdot}100g^{-1}\;DW$ and $14.2-1217.0mg{\cdot}100g^{-1}\;DW$, respectively. In addition, we selected that 8 egg plant with the superior genotypes for antioxidant activity, polyphenol, and flavonoid content. Results revealed that there was significant variation of antioxidant activity and bioactive compounds contents in both vegetable famaily. In addition, we suggested that selected genotypes seeds with high contain bioactive compounds will be more efficiency to develop natural value-added products.

Sires' MC1R Genotypes and Coat Color of the Offspring of the Chikso (Korean Brindle Cattle) (칡소 씨수소의 MC1R 유전자형과 자손의 모색 발현)

  • Park, Jae-Hee;Lee, Chang-Woo;Lee, Hae-Lee;Choi, Jae Won;Choy, Yun Ho;Kwon, A-Nam;Ji, Yeoen Hee;Kim, Jong Gug
    • Journal of Embryo Transfer
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    • v.29 no.1
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    • pp.21-27
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    • 2014
  • The objective of this study was to determine the effect of the MC1R genotypes of the Chikso (Korean brindle cattle) sires on the coat colors of their offspring. In this study, 15 Chikso sires with known MC1R genotypes were used for breeding in the Gangwon Province Livestock Research Center, the Chungbuk Institute of Livestock and Veterinary Research, and the Livestock Experiment Station, Jeonbuk Institute of Livestock and Veterinary Research from either 2011 or 2012 to 2013. There were 6 sires with $E^+E^+$ genotypes and 9 sires with $E^+e$ genotypes, and their coat colors were all whole brindle (more than 50 of the body). Among the 90 calves produced in 2011~2013 or 2012~2013 from the 15 sires, 50 (55.6%) of them were females and 40 (44.4%) of them were males. Coat colors of the offspring were determined when they reached over 6 months of age. Calves with whole brindle, part brindle, brown and black coat colors were 42 (48.3%), 11 (12.6%), 18 (20.7%) and 16 (18.4%), respectively. Ratio of calves with whole brindle coat color was higher than any other coat colors. Among the offspring with whole brindle color, 20 (41.7%) calves were female and 22 (51.3%) calves were male. By determining the MC1R genotypes of the dams and calves in this study along the family lines, and investigating other genes that may be involved in the coat colors of the Chikso, better breeding system may be established to increase the brindle coat color appearance in the future.

Study on the Relationship between Polymorphisms in Glutathione S-transferase and Ischemic Cerebrovascular Disease

  • Han Sang-Hyuk;Park Sae-Wook;Shin Yong-Il;Cho Kwang-Ho;Moon Byung-Soon
    • The Journal of Korean Medicine
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    • v.25 no.4
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    • pp.36-42
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    • 2004
  • Objective : Glutathione S-transferase polymorphism (GST) were examined in 120 cases with ischemic cerebrovascular disease (ICVD) to test the hyperthesis that GST polymorphisms confer a risk to an individual to develop ICVD. Tobacco smoking is a major cause of both cancer and vascular disease. Methods : therefore We were stratified the subjects with ICVD for smoking status, and then examined whether polymorphisms in this detoxification enzyme gene, GST, influence risk of ICVD Results : Neither GSTM1 nor GSTT1 genotypes in the ICVD group was significantly different from the control group (n=207), even in smokers. We attempted the combined analyses for GSTM1 and GSTT1 genotypes in ICVD for smoking status. No significant association observed between the combined genotypes and ICVD Conclusion : Our observation do not confirm the effect of the GSTM1 and GSTT1 genotypes as a risk factor for ICVD, even in smokers.

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Genetic Deletions of GSTM1 and GSTT1 in Head and Neck Cancer: Review of the Literature from 2000 to 2012

  • Masood, Nosheen;Yasmin, Azra;Kayani, Mahmood A.
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3535-3539
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    • 2013
  • Head and neck cancer is one of the leading causes of deaths worldwide. Two genes GSTM1 and GSTT1 involved in phase II of carcinogen detoxification have been frequently studied in the literature. Their null genotypes are thought to be associated with increased head and neck cancer risk. However, the published reviews are not up to date and many important papers have been skipped. The current literature review was restricted to the null genotypes of the GSTM1 and GSTT1 genes with special emphasis on the genotypic status. We found that the size of study sample varied greatly and the oral cavity cancer was more influenced by GSTM1 and GSTT1 gene deletions. With respect to ethnicity Asians are more prone to head and neck cancers with these null genotypes as compared to Europeans and Americans. The current review showed significant associations (OR=9.0, 95%CI; 1.4-9.5; OR=3.7, 95%CI; 1.4-9.5) of GSTM1 and GSTT1 null genotypes with head and neck cancers. Review confirms the data of previous reviews that GSTM1 and GSTT1 gene polymorphisms may be risk factors for cancer initiation.