The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Response of Barley Genotypes to Fusarium Head Blight under Natural Infection and Artificial Inoculation Conditions

  • Khanal, Raja (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Choo, Thin Meiw (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Xue, Allen G. (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Vigier, Bernard (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Savard, Marc E. (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Blackwell, Barbara (Ottawa Research and Development Centre, Agriculture and Agri-Food Canada) ;
  • Wang, Junmei (Chinese Barley Improvement Centre, Zhejiang Academy of Agricultural Sciences) ;
  • Yang, Jianming (Chinese Barley Improvement Centre, Zhejiang Academy of Agricultural Sciences) ;
  • Martin, Richard A. (Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada)
  • Received : 2021.06.08
  • Accepted : 2021.08.08
  • Published : 2021.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of Fusarium graminearum by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of F. graminearum by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (r = 0.47, P < 0.01) and FHB incidence (r = 0.56, P < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (r = 0.12, P > 0.05) under natural infection and it was not correlated with DON concentration (r = -0.23, P > 0.05) and FHB incidence (r = 0.19, P > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.

Keywords

Acknowledgement

The authors were grateful to Professor K. Takeda (Research Institute for Bioresources, Japan), Dr. T. Makino (National Agriculture Research Centre, Japan), Professors S. Ding and S. Xu (Zhejiang Agricultural University, China), Professor D. Rasmussion (University of Minnesota, USA), and Dr. Bill Legge (Brandon Research and Development Centre, Canada) for providing seeds of barley genotypes, and to Yuanhong Chen, Sally Buffam, and Sharon ter Beek for their assistance in conducting this study.

References

  1. Anonymous. 1982. Description of variety: Birka. Agriculture Canada, Ottawa, ON, Canada, pp. 1061-1062.
  2. Anonymous. 1991. Description of variety: Chapais. Agriculture Canada, Ottawa, ON, Canada.
  3. Buerstmayr, H., Legzdina, L., Steiner, B. and Lemmens, M. 2004. Variation for resistance to Fusarium head blight in spring barley. Euphytica 137:279-290. https://doi.org/10.1023/B:EUPH.0000040440.99352.b9
  4. Campbell, K. W., Brawn, R. I. and Ho, K. M. 1984. Rodeo barley. Can. J. Plant Sci. 64:203-205. https://doi.org/10.4141/cjps84-027
  5. Charmley, L. L. and Trenholm, H. L. 2015. Fact sheet: mycotoxins. URL http://www.inspection.gc.ca/animals/feeds/regulatory-guidance/rg-8/eng/1347383943203/1347384015909?chap=0#s1c1 [13 August 2021].
  6. Choo, T. M. 2006. Breeding barley for resistance to Fusarium head blight and mycotoxin accumulation. Plant Breed. Rev. 26:125-169.
  7. Choo, T. M., Ho, K. M., Martin, R. A. and ter Beek, S. 2001a. AC Klinck barley. Can. J. Plant Sci. 81:431-432. https://doi.org/10.4141/P00-127
  8. Choo, T. M., Ho, K. M., Martin, R. A., ter Beek, S. M., Dion, Y. and Rioux, S. 2006a. Chief barley. Can. J. Plant Sci. 86:485-487. https://doi.org/10.4141/P05-084
  9. Choo, T. M., Ho, K. M., ter Beek, S. M., Martin, R. A., Dion, Y. and Rioux, S. 2006b. Encore barley. Can. J. Plant Sci. 86:489-491. https://doi.org/10.4141/P05-085
  10. Choo, T. M., Martin, R. A., Ho, K. M., Bubar, J. S., Walton, R., Blatt, R. and Rodd, V. 1994. AC Sterling barley. Can. J. Plant Sci. 74:817-819. https://doi.org/10.4141/cjps94-147
  11. Choo, T. M., Martin, R. A., Ho, K. M., Shen, Q., Fedak, G., Savard, M., Voldeng, H., Falk, D. E., Etienne, M. and Sparry, E. 2004. Fusarium head blight and deoxynivalenol accumulation of barley in Eastern Canada: cultivar response and correlation analysis. Plant Dis. 88:837-844. https://doi.org/10.1094/PDIS.2004.88.8.837
  12. Choo, T. M., Martin, R. A., Savard, M. E. and Blackwell, B. 2014. Effects of planting date and earliness on deoxynivalenol contamination in barley under natural epidemic conditions. Can. J. Plant Sci. 94:1363-1371. https://doi.org/10.4141/cjps2013-369
  13. Choo, T. M., Martin, R. A., ter Beek, S. M., Caldwell, C. D., Price, M., Rowsell, J., Dion, Y., Rioux, S. and Pageau, D. 2009. Leader barley. Can. J. Plant Sci. 89:921-924. https://doi.org/10.4141/CJPS08232
  14. Choo, T. M., Martin, R. A., ter Beek, S., Ho, K. M., Caldwell, C. D., Walker, D. and Rodd, V. 2001b. AC Alberte hulless barley. Can. J. Plant Sci. 81:425-426. https://doi.org/10.4141/P00-125
  15. Choo, T. M., Martin, R. A., ter Beek, S. M., Ho, K. M., Caldwell, C. D., Walker, D., Rodd, V., Dion, Y. and Rioux, S. 2003. Island barley. Can. J. Plant Sci. 83:793-795. https://doi.org/10.4141/P02-149
  16. Choo, T. M., ter Beek, S. M., Martin, R. A. and Rowsell, J. 2010. AC Minoa. Can. J. Plant Sci. 90:869-871. https://doi.org/10.4141/cjps10064
  17. Cooper, G. W. 1993. Report on eastern six row barley registration test. Plant Research Centre, Agriculture Canada, Ottawa, ON, Canada.
  18. Ding, S., Xu, S., Guan, M. and Huang, J. 1987. Breeding, characteristics, and key cultural practices of barley cultivar Zhenongda 3. Zhejiang Agric. Sci. 6:251-254 (in Chinese).
  19. Falk, D. E. and Reinbergs, E. 1991. OAC kippen spring barley. Can. J. Plant Sci. 71:205-206. https://doi.org/10.4141/cjps91-027
  20. Gao, D. S. 1989. Zaoshu 3. In: Annals of barley cultivars in China, pp. 32-32. Agriculture Press, Beijing, China (in Chinese).
  21. Geddes, J., Eudes, F., Tucker, J. R., Legge, W. G. and Selinger, L. B. 2008. Evaluation of inoculation methods on infection and deoxynivalenol production by Fusarium graminearum on barley. Can. J. Plant Pathol. 30:66-73. https://doi.org/10.1080/07060660809507497
  22. Ho, K. M. 1990. Symko barley. Can. J. Plant Sci. 70:853-854. https://doi.org/10.4141/cjps90-102
  23. Immer, F. R. and Christensen, J. J. 1943. Studies on susceptibility of varieties and strains of barley to Fusarium and Helminthosporium kernel blight when tested under muslin tents or in nurseriess. J. Am. Soc. Agron. 35:515-522. https://doi.org/10.2134/agronj1943.00021962003500060007x
  24. McCallum, B. D., Tekauz, A. and Gilbert, J. 2004. Reaction of a diverse collection of barley lines to Fusarium head blight. Plant Dis. 88:167-174. https://doi.org/10.1094/PDIS.2004.88.2.167
  25. Rasmusson, D. C., Wilcoxson, R. D. and Wiersma, J. V. 1993. Registration of 'Stander' barley. Crop Sci. 33:1403. https://doi.org/10.2135/cropsci1993.0011183X003300060057x
  26. Rossnagel, B. G., Bhatty, R. S., Scoles, G. J. and Harvey, B. L. 1998. CDC Freedom 2-row hulless barley. URL http://wheat.pw.usda.gov/ggpages/BarleyNewsletter/41/CDCFreedom.html [13 August 2021].
  27. Sameri, M., Takeda, K. and Komatsuda, T. 2006. Quantitative trait loci controlling agronomic traits in recombinant inbred lines from a cross of oriental- and occidental-type barley cultivars. Breed. Sci. 56:243-252. https://doi.org/10.1270/jsbbs.56.243
  28. SAS Institute. 2004. SAS system viewer 9.1. SAS Institute Inc., Cary, NC, USA.
  29. Shands, R. G. 1939. Chevron, a barley variety resistant to stem rust and other diseases. Phytopathology 29:209-211.
  30. Sinha, R. C. and Savard, M. E. 1996. Comparison of immunoassay and gas chromatography methods for the detection of the mycotoxin deoxynivalenol in grain samples. Can. J. Plant Pathol. 18:233-236. https://doi.org/10.1080/07060669609500617
  31. Sinha, R. C., Savard, M. E. and Lau, R. 1995. Production of monoclonal antibodies for the specific detection of deoxynivalenol and 15-acetyldeoxynivalenol by ELISA. J. Agric. Food. Chem. 43:1740-1744. https://doi.org/10.1021/jf00054a061
  32. Takeda, K. and Heta, H. 1989. Establishing the testing method and a search for the resistant varieties to Fusarium head blight in barley. Jpn. J. Breed. 39:203-216 (in Japanese). https://doi.org/10.1270/jsbbs1951.39.203
  33. Urrea, C. A., Horsley, R. D., Steffenson, B. J. and Schwarz, P. B. 2005. Agronomic characteristics, malt quality, and disease resistance of barley germplasm lines with partial Fusarium head blight resistance. Crop Sci. 45:1235-1240. https://doi.org/10.2135/cropsci2003.0608
  34. Vivar, H. E., Gilchrist, L., Hayes, P., Zongzhen, L., Steffenson, B., Franco, J. and Henry, M. 1997. Head scab resistant barley for malting and food. Cereal Res. Commun. 25:693-697. https://doi.org/10.1007/BF03543815
  35. Xue, A. G., Armstrong, K. C., Voldeng, H. D., Fedak, G. and Babcock, C. 2004. Comparative aggressiveness of isolates of Fusarium spp. causing head blight on wheat in Canada. Can. J. Plant Pathol. 26:81-88. https://doi.org/10.1080/07060660409507117
  36. Yoshida, M., Kawada, N. and Nakajima, T. 2007. Effect of infection timing on fusarium head blight and mycotoxin accumulation in open- and closed-flowering barley. Phytopathology 97:1054-1062. https://doi.org/10.1094/PHYTO-97-9-1054
  37. Zhou, X., Chao, M. and Liang, X. 1991. Screening and testing of barley varieties for scab resistance Acta Phytophylacica Sin. 18:261-265 (in Chinese).