• Interdisciplinary Bio Central
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• 제2권4호
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• pp.12.1-12.7
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• 2010

The National Bio Resource Project-Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

• Journal of Ginseng Research
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• 제32권4호
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• pp.300-304
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• 2008

14년생 인삼의 뿌리로부터 cDNA library를 제작한 후 무작위로 뽑은 EST clone 중에서 cysteine proteinase(CP) 유전자에 높은 상동성을 나타내는 clone 6개를 선발하였고 이를 바탕으로 PgCysP1을 제작하였다. 인삼의 CP 유전자는 전장의 길이가 1,398bp로 366개의 아미노산을 코딩하는 1,101bp의 ORF를 가지고 있다. PgCysP1의 아미노산 서열과 이차구조를 분석한 결과, 기존에 보고된 식물들과 높은 상동성을 나타내었으며 PgCysP1는 papain family에 속하는 것으로 확인되었다. RT-PCR 분석결과, 인삼의 PgCysP1는 NaCl, 저온, wound, salicylic acid에 대해 그 발현 양상이 상동성을 나타내는 다른식물의 CP 유전자 발현과 유사한 양성을 보였고, 이에 PgCysP1은 CP gene으로 사료되며, 앞으로 인삼 식물체의 환경 스트레스에 대한 내성 연구가 요구된다.

• 한국약용작물학회지
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• 제16권2호
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• pp.94-99
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• 2008

인삼의 생장에서 염류의 집적은 우량 인삼의 생산에 많은 장애요인이 되고 있다. 본 연구에서는 순계 분리된 인삼의 우수 계통으로부터 NaCl 처리에 따른 생장율 조사와 ginsenoside의 생산에 미치는 영향을 조사하였다. 선발된 모상근(KGHR-8)으로부터 ginsenosides의 함량에 미치는 NaCl의 최적 농도를 조사하기 위하여 30일간 배양한 결과 NaCl의 농도가 증가함에 따라 모상근의 생장은 감소하였지만, total ginsenoside의 함량은 0.24M NaCl 처리구에서 높은 증가를 가져왔으며 특히 광을 조사하여 배양한 결과 높게 검출되었다. 0.24 M NaCl 농도로 광상태하에서 함량은 61.7% 증가하는 양상을 나타내었다. (Table 1). 또한 모상근의 생장을 최적 상태로 설정하기 위해 two step culture 방법을 조사한 결과, 0.05M, 0.1M NaCl 처리시 모상근의 생장율은 각각 약 62%, 76% 감소한 반면, ginsenoside의 함량은 29%, 48% 각각 향상되었다. 모상근은 방어기작의 일환으로 NaCl을 elicitor로 인지하고 2차대사산물인 사포닌의 생산에 영향을 미치는 것으로 확인되었다.

• Animal Systematics, Evolution and Diversity
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• 제26권1호
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• pp.15-19
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• 2010

The Russian ratsnake, Elaphe schrenckii, is found in Russia, China, and Korea, and is considered to be an endangered species by the Ministry of Environment in South Korea. Due to habitat loss and use in oriental medicine, their population has been severely decimated. In South Korea, two subspecies of E. schrenckii has been defined according to body color: E. s. schrenckii (blackish) and E. s. anomala (yellow-brownish). Molecular genetic studies on Elaphe schrenckii are very scarce and the taxonomy of Elaphe schrenckii subspecies is uncertain. From the present study, we attempted to identify the genetic differences of these two subspecies using species-specific microsatellites developed from the genomic library of E. schrenckii. Nine polymorphic loci were tested on 19 individuals from E. s. schrenckii (n=10) and E. s. anomala (n=9) in South Korea. The mean number of alleles was 3.78 in E. s. schrenckii and 4.11 in E. s. anomala. The average expected heterozygosity was 0.542 and 0.511 in E. s. schrenckii and E. s. anomala, respectively. We found a lack of genetic structure between two subspecies ($F_{ST}=0.016$) and no genetic discrimination between two subspecies was found. Based on the present findings by microsatellites, two subspecies can be considered as one species, E. schrenckii. However, further investigations on taxonomical status using mitochondrial and nuclear DNA sequences need to be performed and morphological & ecological data should be revised. The genetic markers should benefit future studies of the endangered species of other Elaphe species for the study of genetic diversity and potential conservation management.

• 한국통신학회논문지
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• 제33권11A호
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• pp.1095-1104
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• 2008

다중 사용자 OFDM 시스템에서 제한된 자원을 효율적으로 사용하기 위해서는 부반송파와 비트의 할당은 중요한 역할을 한다. 하지만 부반송파와 비트의 할당문제는 비선형적 문제로 모든 경우의 수를 계산하여 최적의 값을 얻기에는 사실상 불가능하다. 본 논문에서는 비선형적 문제의 효율적인 자원 활용을 위해서 새로운 유전자 알고리즘을 사용하였다. 논문에서 제안된 알고리즘은 기존의 정형화된 유전자 알고리즘보다 다양한 조합을 참고하여 해를 찾게 된다. 따라서 수치적 시뮬레이션 결과들을 통해서 기존의 알고리즘들과 제안된 알고리즘을 비교해 볼 때, 제안한 알고리즘이 기존의 알고리즘들보다 뛰어난 성능을 보임을 확인하였다.

• Genomics & Informatics
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• 제8권3호
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• pp.101-102
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• 2010

During the last decade, large community cohorts have been established by the Korea National Institutes of Health (KNIH), and enormous epidemiological and clinical data have been accumulated. Using these information and samples in the cohorts, KNIH set out to do a large-scale genome-wide association study (GWAS) in 2007, and the Korea Association REsource (KARE) consortium was launched to analyze the data to identify the underlying genetic risk factors of diseases and diverse health indexes, such as blood pressure, obesity, bone density, and blood biochemical traits. The consortium consisted of 6 research divisions, formed by 25 principal investigators in 19 organizations, including 18 universities, 2 institutes, and 1 company. Each division focused on one of the following subjects: the identification of genetic factors, the statistical analysis of gene-gene interactions, the genetic epidemiology of gene-environment interactions, copy number variation, the bioinformatics related to a GWAS, and a GWAS of nutrigenomics. In this special issue, the study results of the KARE consortium are provided as 9 articles. We hope that this special issue might encourage the genomics community to share data and scientists, including clinicians, to analyze the valuable Korean data of KARE.

• International Journal of Computer Science & Network Security
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• 제21권12호
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• pp.175-182
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• 2021

Deadlock is a situation in which two or more processes competing for resources are waiting for the others to finish, and neither ever does. There are two different forms of systems, multi-unit and single-unit resource systems. The difference is the number of instances (or units) of each type of resource. Deadlock problem can be modeled as a constrained combinatorial problem that seeks to find a possible scheduling for the processes through which the system can avoid entering a deadlock state. To solve deadlock problem, several algorithms and techniques have been introduced, but the use of metaheuristics is one of the powerful methods to solve it. Genetic algorithms have been effective in solving many optimization issues, including deadlock Problem. In this paper, an improved parallel framework of the genetic algorithm is introduced and adapted effectively and efficiently to deadlock problem. The proposed modified method is implemented in java and tested on a specific dataset. The experiment shows that proposed approach can produce optimal solutions in terms of burst time and the number of feasible solutions in each advanced generation. Further, the proposed approach enables all types of crossovers to work with high performance.

• 경영과정보연구
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• 제35권3호
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• pp.95-113
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• 2016

본 연구는 유전자은행을 통한 유전자원활용을 극대화하기 위한 맥락에서 유전자은행의 유전자원이용에 대한 만족과 지속적 이용의 정도차이를 판별할 수 있는 요인, 즉 유전자원이용자(=연구자)가 유전자원에 부여하는 유용성 속성을 실증적으로 분석하고자 하였다. 이를 위해 기존문헌 고찰을 통해 연구자들이 유전자원에 부여하는 유용성 속성을 연구 활동에의 부합성, 유용특성정보, 활용가치, 연구 활동의 촉진성, 신규성으로 도출하고, 미생물유전자원을 이용하는 연구자들을 대상으로 유전자원이용정도가 높은 집단과 낮은 집단(유전자원이용에 대한 만족과 지속적 이용에 기초하여 분류)을 판별할 수 있는 유용성 속성의 파악을 위해 판별분석을 수행하였다. 분석결과, 5개의 유용성 속성 중에서 유전자원의 활용가치와 연구 활동에의 부합성이 판별력이 가장 높은 것으로 나타났다. 유용특성정보와 연구 활동의 촉진성은 판별력이 그리 높지 않았으며, 신규성은 판별력이 없는 것으로 나타났다. 본 연구의 결과에 기초하여 유전자원의 활용촉진을 위한 정책적 시사점을 제공하였으며, 본 연구의 한계점과 이를 극복하기 위한 향후 연구방향도 제시하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권1호
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• pp.1-7
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• 2017

Objective: Three genome-wide association studies (GWAS) and a meta-analysis of GWAS were conducted to explore the genetic mechanisms underlying variation in pig teat number. Methods: We performed three GWAS and a meta-analysis for teat number on three pig populations, including a White Duroc${\times}$Erhualian $F_2$ resource population (n = 1,743), a Chinese Erhualian pig population (n = 320) and a Chinese Sutai pig population (n = 383). Results: We detected 24 single nucleotide polymorphisms (SNPs) that surpassed the genome-wide significant level on Sus Scrofa chromosomes (SSC) 1, 7, and 12 in the $F_2$ resource population, corresponding to four loci for pig teat number. We highlighted vertnin (VRTN) and lysine demethylase 6B (KDM6B) as two interesting candidate genes at the loci on SSC7 and SSC12. No significant associated SNPs were identified in the meta-analysis of GWAS. Conclusion: The results verified the complex genetic architecture of pig teat number. The causative variants for teat number may be different in the three populations

• Molecules and Cells
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• 제22권3호
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• pp.323-327
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• 2006

DNA barcoding, an inventory of DNA sequences from a standardized genomic region, provides a bio-barcode for identifying and discovering species. Several recent studies suggest that the sequence diversity in a 648 bp region of the mitochondrial gene for cytochrome c oxidase I (COI) might serve as a DNA barcode for identifying animal species such as North American birds, insects and fishes. The present study tested the effectiveness of a COI barcode in discriminating Korean bird species. We determined the 5' terminus of the COI barcode for 92 species of Korean birds and found that species identification was unambiguous; the genetic differences between closely related species were, on average, 25 times higher than the differences within species. We identified only one misidentified species out of 239 specimens in a genetic resource bank, so confirming the accuracy of species identification in the banking system. We also identified two potential composite species, calling for further investigation using more samples. The finding of large COI sequence differences between species confirms the effectiveness of COI barcodes for identifying Korean bird species. To bring greater reliability to the identification of species, increased intra- and interspecies sampling, as well as supplementation of the mitochondrial barcodes with nuclear ones, is needed.