• 한국약용작물학회지
• /
• 제16권2호
• /
• pp.74-78
• /
• 2008

감초의 에틸아세테이트 분획 중 flavonoid 지표물질인 liquiritin이 들어있는 분획 (GUE6)에 살구 및 복숭아 종자로부터 얻은 조효소액 (PDE, PAE, PPE)을 법제 처리하였다. 각 조효소액에서 ${\beta}-glucosidase$ 활성도는 아몬드 (P. dulcis) 259.6 U/g 살구 (P. persica), 복숭아 (P. persica) 조효소액의 ${\beta}-glucosidase$ 활성도가 가장 높게 관찰되었다. PDE, PAE, PPE를 이용한 발효 법제 후 liquiritigenin의 함량 비교 결과, 효소중의 ${\beta}-glucosidase$에 의해 liquiritin이 대사되어 항산화, 향군, 세포 독성 억제, 항치매, 항피부암 등 많은 약리효능을 가진 활성 물질인 liquiritigenin이 생산됨이 확인되었으며, 세효수 모두 liquiritin에 1.2배의 효소 처리 시 가장 대사가 활발한 것으로 나타나 변환을 위한 최적 농도로 결정되었다 세효소 중 특히 PPE 처리 시 liquiritin이 모두 liquiritigenin으로 변환됨으로써 liquiritin의 변환에 복숭아 종자 유래 효소가 가장 효율적인 것으로 밝혀졌다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
• /
• pp.15-15
• /
• 2010

Ginseng contained many different kinds of saponin which was the most valuable for people, but its yield cannot satisfy the demand using traditional extract methods. Enzyme transformation is a conformable and highly performed method which was fit for today. A ${\beta}$-glucosidase producing bacterium ($DCY51^T$) was isolated from Korean fermented-vegetable food kimchi. The 16S rRNA gene sequence analysis revealed that the strain $DCY51^T$ belongs to the genus Lactobacillus. The highest sequence similarity was found with Lactobacillus paracollinoides LMG $22473^T$ and Lactobacillus collinoides LMG $9194^T$ with levels of 16S rDNA similarity of 97.4% and 97.3%, respectively. Based on the above results the strain $DCY51^T$ placed in the genus Lactobacillus and proposed a new species, Lactobacillus kimchicus sp. nov. $DCY51^T$ (= KCTC $12976^T$ = JCM $15530^T$). It was culture solution reacted with Red Ginseng extract and $Rb_1$, respectively. The medium of bacteria was the liquid of MRS, the temperatures of growing and reacting between bacteria liquid and saponin were samely $37^{\circ}C$, there spective reacting time were 12 hours and 48 hours. Thus we got different saponins, and TLC and HPLC analysis showed that: enzyme respectively reacted with $Rb_1$ and Red Ginseng extract got the transformed saponin, respectively. The polarity position in TLC was a little higher than Rd; and the polarity position was the same as that of Compound K's, the saponin obtained from HPLC and other experimental results was not Compound K. The constitution of its saponin was hoped to be further confirmed.

• Journal of Ginseng Research
• /
• 제39권2호
• /
• pp.141-147
• /
• 2015

Background: Adipocytes, which are the main cellular component of adipose tissue, are the building blocks of obesity. The nuclear hormone receptor $PPAR{\gamma}$ is a major regulator of adipocyte differentiation and development. Obesity, which is one of the most dangerous yet silent diseases of all time, is fast becoming a critical area of research focus. Methods: In this study, we initially aimed to investigate whether the ginsenoside Rf, a compound that is only present in Panax ginseng Meyer, interacts with $PPAR{\gamma}$ by molecular docking simulations. After we performed the docking simulation the result has been analyzed with several different software programs, including Discovery Studio, Pymol, Chimera, Ligplus, and Pose View. All of the programs identified the same mechanism of interaction between $PPAR{\gamma}$ and Rf, at the same active site. To determine the drug-like and biological activities of Rf, we calculate its absorption, distribution, metabolism, excretion, and toxic (ADMET) and prediction of activity spectra for substances (PASS) properties. Considering the results obtained from the computational investigations, the focus was on the in vitro experiments. Results: Because the docking simulations predicted the formation of structural bonds between Rf and $PPAR{\gamma}$, we also investigated whether any evidence for these bonds could be observed at the cellular level. These experiments revealed that Rf treatment of 3T3-L1 adipocytes downregulated the expression levels of $PPAR{\gamma}$ and perilipin, and also decreased the amount of lipid accumulated at different doses. Conclusion: The ginsenoside Rf appears to be promising compound that could prove useful in antiobesity treatments.

• 한국콘텐츠학회논문지
• /
• 제11권6호
• /
• pp.59-66
• /
• 2011

자원 제약이 있는 프로젝트 스케줄링 문제는 자원의 양은 제한되어 있고 작업들 간에 선행조건이 있는 일정계획 문제로서 NP-hard 문제 중에 하나로 알려져 있다. 이러한 문제는 결정론적인 방법을 사용해서는 주어진 시간 내에 최적해를 구하기 어렵기 때문에 근사 최적해를 빠른 시간에 구할 수 있는 휴리스틱 방법을 이용한다. 본 논문에서는 자원 제약이 있는 프로젝트 스케줄링 문제를 효율적으로 해결할 수 있는 유전알고리즘을 소개한다. 제안한 유전알고리즘은 스키마 이론을 적용한 교차 연산자와 실세계 토너먼트 선택 전략을 이용하였다. 표준 문제에 실험한 결과는 제안한 알고리즘이 기존의 알고리즘 보다 우수함을 보여주었다.

• Interdisciplinary Bio Central
• /
• 제2권4호
• /
• pp.13.1-13.8
• /
• 2010

The National BioResource Project of Japan is a governmental project to promote domestic/international research activities using biological resources. The project has 27 biological resources including three cereal resources. The core center and sub-center which historically collected the cereal resources were selected for each cereal program. These resources are categorized into several different types in the project; germplasm, genetic stocks, genome resources and database information. Contents of rice resources are wild species, local varieties in East and Southwest Asia & wild relatives, MNU-induced chemical mutant lines, marker tester lines, chromosome substitution lines and other experimental lines. Contents of wheat resources are wild strains, cultivated strains, experimental lines, rye wild and cultivated strains; EST clones and full-length cDNA clones. Contents of barley resources are cultivar and experimental lines, core collection, EST/cDNA clones, BAC clones, their filters and superpool DNA. Each resource is accessible from the online database to see the contents and information about the resources. Links to the genome information and genomic tools are also important function of each database. The major contents and some examples are presented here.

• 한국정보통신학회논문지
• /
• 제20권8호
• /
• pp.1415-1421
• /
• 2016

무기체계의 기능 및 성능 검증을 위한 시험들이 지속적으로 증가함에 따라 가용 자원들의 효율적인 활용을 위한 방안에 관련된 연구가 대두되고 있으며, 자원할당 복잡도가 증가함에 따라 시험계획 시에 의사결정 지원이 요구되고 있다. 시험자원할당은 전통적인 FJSP(Flexible Job Shop Problem)와 기본적으로 동일한 문제이며, 이는 NP-hard문제로서 기존의 경험기반 시험자원 할당 방법으로는 시간 효율적인 자원할당에 있어서 한계가 존재한다. FJSP에 유전자알고리즘을 적용한 최적해 탐색 연구가 진행되어 왔지만, 하나의 기계조작에 대해 두 개 이상 기계의 동시 작동이 필요한 시험자원할당 도메인에서의 적용은 제한적이다. 이에 본 논문에서는 가변 그룹 유전자알고리즘을 제안한다. 제안하는 알고리즘은 수작업 기반의 기존 시험자원할당을 자동화하고 최적화함으로써 시험 효율을 향상시킬 것으로 기대되며, MATLAB을 이용한 시뮬레이션을 통해 그 적용성을 확인하였다.

• 국제학술발표논문집
• /
• The 9th International Conference on Construction Engineering and Project Management
• /
• pp.196-203
• /
• 2022

The resource constrained scheduling problem (RCSP) constitutes one of the most challenging problems in Project Management, as it combines multiple parameters, contradicting objectives (project completion within certain deadlines, resource allocation within resource availability margins and with reduced fluctuations), strict constraints (precedence constraints between activities), while its complexity grows with the increase in the number of activities being executed. Due to the large solution space size, this work investigates the application of Genetic Algorithms to approximate the optimal resource alolocation and obtain optimal trade-offs between different project goals. This analysis uses the cost of exceeding the daily resource availability, the cost from the day-by-day resource movement in and out of the site and the cost for using resources day-by-day, to form the objective cost function. The model is applied in different case studies: 1 project consisting of 10 activities, 4 repetitive projects consisting of 40 activities in total and 16 repetitive projects consisting of 160 activities in total, in order to evaluate the effectiveness of the algorithm in different-size solution spaces and under alternative optimization criteria by examining the quality of the solution and the required computational time. The case studies 2 & 3 have been developed by building upon the recurrence of the unit/sub-project (10 activities), meaning that the initial problem is multiplied four and sixteen times respectively. The evaluation results indicate that the proposed model can efficiently provide reliable solutions with respect to the individual goals assigned in every case study regardless of the project scale.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2015년도 춘계 학술논문 발표대회
• /
• pp.132-133
• /
• 2015

Efficient scheduling and resource management are the key factor to reduce construction project budget (e.g., labor cost, equipment cost, material cost, etc.). Resource-based line of balance (LOB) technique has been used to complement the limitations of time-driven scheduling techniques (e.g., critical-path method). Optimizing construction alternatives contributes cost savings while honoring the project deadline. However, existing LOB scheduling is lack of identifying optimal resource combination. This study presents a method which identifies the optimal construction alternatives, hence achieving resource minimization in a repetitive construction by using genetic algorithm (GA). The method provides efficient planning tool that enhances the usability of the system.

• 국제학술발표논문집
• /
• The 6th International Conference on Construction Engineering and Project Management
• /
• pp.158-160
• /
• 2015

Efficient scheduling and resource management are the key factor to reduce construction project budget (e.g., labor cost, equipment cost, material cost, etc.). Resource-based line of balance (LOB) technique has been used to complement the limitations of existing time-driven scheduling techniques (e.g., critical-path method). Optimizing construction alternatives contributes to cost savings while honoring the project deadline. However, existing LOB scheduling is lack of identifying optimal resource combination. This study presents a method which identifies the optimal construction alternatives, hence achieving resource minimization in a repetitive construction by using genetic algorithm (GA). The method provides efficient planning tool that enhances the usability of the system.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권4호
• /
• pp.574-579
• /
• 2014

A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific activity greater than 500 mg/L and 4,000 U/mg was obtained. Results at optimal pH and temperature showed recombinant lysozyme has higher lytic activity at pH 6.5 and $45^{\circ}C$. This study demonstrates the successful expression of recombinant lysozyme using the eukaryotic host organism P. pastoris paving the way for protein engineering. Additionally, this study shows the feasibility of subsequent industrial manufacture of the enzyme with this expression system together with a high purity scheme for easy high-yield purification.