• 한국약용작물학회지
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• 제16권4호
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• pp.218-224
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• 2008

국내자생 쑥속의 식물을 약용작물 및 산업화에 활용하기 위한 기초자료를 얻고자 본 연구를 수행하였다. 그 결과, 수집된 쑥속 식물들은 사철쑥 (A. capillaris)을 포함한 20종 1아종 2변종 24분류군으로 분류 되었으며, 이를 바탕으로 25개의 화기형질을 이용하여 주성분 분석과 군집분석을 수행하였다. 주성분 분석결과 제1주성분은 전체 분산의 44.73%, 제2주성분은 16.86%, 제 3주성분은 8.88%, 제4주성분은 7.07%의 기여율을 보였으며, 상위 제4주성분까지의 누적 기여율이 77.56%였다. 군집분석 결과, 자방의 퇴화, 아관목, 두화의 크기 등의 주요형질에 의해 크게 3개의 군으로 구분되어졌으며, 화기구조의 식별형질로는 기발표된 Dracunculus, Abrotanum, Absinthium 3절과 완전히 일치하지는 않았으나 국내 자생쑥의 분류형질로 활용이 가능하였다.

• 생명과학회지
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• 제16권1호
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• pp.12-16
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• 2006

항암유전자 Brca1의 변이는 유방암 및 난소암에 대한 감수성을 증가시키며, Brca1은 DNA손상신호후 세포주기 조절에 필수적인 역할을 한다. 연구결과, Brca1이 세포주기 S기와 G2/M 조절점에서 중요한 역할을 담당함이 밝혀졌다. 그러나, Brca1의 spindle checkpoint 관여여부는 알려져 있지 않다. 본 연구에서는 spindle checkpoint를 활성화시키는 nocodazole를 처리하여 야생형, $p53^{-/-}$ 그리고 $p53^{-/-}\;Brca1^{-/-}$ 세포주의 세포주기 변화를 조사하였다. 야생형과 $p53^{-/-}$ 세포주는 신속한 mitosis기 정지가 나타난 반면, $p53^{-/-}\;Brca1^{-/-}$ 세포주의 경우 모든 세포가 M기에서 정지하지 않았다. Double-thymidine block 기법에 의한 세포주기 동조화후 nocodazole 처리시에도 $p53^{-/-}\;Brca1^{-/-}$ 세포주에서는 일부세포가 M기 조절점을 통과하여 계속 G1기로 진행하였다. 형태학적 분석에서도 nocodazole 함유배지에서 계속 증식하는 세포형태가 관찰되었다. 이와 같은 결과들은 Brca1이 spindle checkpoint가 정상적으로 작동하는데 중요한 역할을 담당한다는 것을 의미하고 있다.

• 생약학회지
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• 제24권4호
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• pp.267-281
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• 1993

To find antitumor components from higher fungi, the mycelia of Collybia confluens (Pers. ex Fr.) Kummer were cultured in artificial media. For efficient production of the mycelia, the influences of various modifications of culture conditions were examined. A water-soluble protein-bound polysaccharide fraction, Fr. A, was obtained from the mycelia by hot water extraction. When Fr. A was purified and fractionated by DEAE-cellulose and Sepbadex G-200 gel filtration chromatographies into four fractions which were designated B, C, C-I and C-II. The tumor inhibition ratios of these fractions ranged from 46% to 75% against the solid forms of sarcoma 180 in ICR mice at doses of 20 and 50 mg/kg/day when given intraperitoneally. Especially, Fr. C which was named Collyban(CB) exhibited a marked life-prolonging effect of the mice against ascitic forms of sarcoma 180 at a dose of 50 mg via i.p. administration. To extend spectra of the antitumor activities and eliminate the effects of allograft rejection, the characterization of antitumor effects of CB was performed in syngeneic host-tumor systems. It did not show any antitumor activity against L1210 murine leukemia in $CD_2Fl$ mice but prolonged their life span against ascitic forms of $MM_{46}$ carcinoma in $C_3H/He$ mice. Also it exhibited antitumor activity against human cervical cancer HeLa cells that were xenografted into nude mice having BALB/c genetic backgrounds by the i.p. injection at a dose of 100 mg/kg/day. In order to characterize the antitumor components, CB was examined by chemical analysis. It was acidic protein-bound polysaccharides composed of 31% polysaccharide, 27% protein and 3% hexosamine. CB was fractionated into two fractions, Fr. C-I(M.W.: 500 Kd) and Fr. C-II(M.W.:30 and 8 Kd) by Sephadex G-200 gel filtration chromatography.

• 대한치과교정학회지
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• 제49권1호
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• pp.3-11
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• 2019

Objective: The purpose of this study was to investigate the influence of heritability on the craniofacial soft tissue cephalometric characteristics of monozygotic (MZ) twins, dizygotic (DZ) twins, and their siblings (SIB). Methods: The samples comprised Korean adult twins and their siblings (mean age, 39.8 years; MZ group, n = 36 pairs; DZ group, n = 13 pairs of the same gender; and SIB group, n = 26 pairs of the same gender). Thirty cephalometric variables were measured to characterize facial profile, facial height, soft-tissue thickness, and projection of nose and lip. Falconer's method was used to calculate heritability (low heritability, $h^2$ < 0.2; high heritability, $h^2$ > 0.9). After principal components analysis (PCA) was performed to extract the models, we calculated the intraclass correlation coefficient (ICC) value and heritability of each component. Results: The MZ group exhibited higher ICC values for all cephalometric variables than DZ and SIB groups. Among cephalometric variables, the highest ${h^2}_{(MZ-DZ)}$ and ${h^2}_{(MZ-SIB)}$ values were observed for the nasolabial angle (NLA, 1.544 and 2.036), chin angle (1.342 and 1.112), soft tissue chin thickness (2.872 and 1.226), and upper lip thickness ratio (1.592 and 1.026). PCA derived eight components with 84.5% of a cumulative explanation. The components that exhibited higher values of ${h^2}_{(MZ-DZ)}$ and ${h^2}_{(MZ-SIB)}$ were PCA2, which includes facial convexity, NLA, and nose projection (1.026 and 0.972), and PCA7, which includes chin angle and soft tissue chin thickness (2.107 and 1.169). Conclusions: The nose and soft tissue chin were more influenced by genetic factors than other soft tissues.

• 한국버섯학회지
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• 제20권4호
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• pp.218-226
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• 2022

팽이는 국내 생산량이 느타리, 큰느타리 다음으로 많고, 수출량도 버섯 전체 수출량의 60%이상 차지할 정도로 대표적인 버섯이며 가격이 저렴하여 소비자들이 쉽게 접근할 수 있는 품목이다. 그러나 팽이는 소비자들에게는 단순한 부재료로 인식되어 수요를 높이기에는 한계가 있다. 이에 팽이의 소비한계를 극복하고 품종의 차별성을 높이고자 기능성이 강화된 품종 개발이 필요하다. 따라서 본 연구에서는 팽이의 기호성과 기능성이 높은 육종소재를 발굴하고자 유전자원 26점을 대상으로 아미노산, 무기물을 포함하는 일반 영양성분, 베타글루칸 및 폴리페놀, 지방산 5종 및 GABA 물질까지 분석하였다. 칼슘, 칼륨, 마그네슘과 같은 무기물과 아미노산 15종에서 4148 균주가 가장 함량이 높았다. 면역 활성을 높이는 베타글루칸과 항산화 효능이 있는 폴리페놀은 일부 균주를 제외하고는 유색자원이 흰색자원들에 비해 높은 경향성을 보였다. 지방산은 5가지 중 오메가-6 지방산인 리놀렌산과 오메가3-지방산 중 하나인 알파-리놀렌산이 버섯에서 검출되었으며 특히, 알파-리놀렌산은 느타리 등 5가지 주요버섯에는 없고 팽이에만 유일하게 확인되었다. 신경안정에 도움이 되는 GABA는 유색자원에서는 '아람'(4232) 품종이 15.38 ㎍/ml으로 가장 높았으며, 흰색은 '백승'(4230) 품종이 20.56 ㎍/ml으로 가장 높았다. 본 연구결과는 기능성이 강화된 팽이 신품종을 개발하는데 유용한 자료로 활용될 것으로 기대된다.

• 한국작물학회지
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• 제37권5호
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• pp.431-435
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• 1992

벼의 기원지 아닌 우리나라에서도 재배도와 형태적으로 유사한 적미가 준야성의 장태로 잔존하므로 전국에서 이들을 1,113계통 수집하여 유용 유전 자원으로서의 활용성을 탐색하고자 853계통에 대하여 수량구성요소, 간장 및 수장을 조사한 결과를 요약하면 다음과 같다. 1. 장ㆍ단립형 적미는 수수는 각각 평균 21.5, 11.8로서 장립형적미가 약 2배 많았다. 2. 장ㆍ단립형 적미의 이삭당영화수는 각각 평균 86.1, 108.7로서 단립형 적미가 많았다. 3. 장ㆍ단립형 적미의 천립중은 각각 20.1g, 20.2g 임실율은 80.4%, 79.4%로서 군간에는 유의차가 없었으며 재배도의 소립종과 비슷한 천립중을 나타내었다. 4. 장ㆍ단립형 적미의 간장은 평균 102.6%cm, 94.8%cm로서 장립형적미가 컸고 양군간에 고도의 유의차가 인정되었다. 5. 장ㆍ단립형 적미의 수장은 각각 평균 22.1cm, 21.3cm로서 양군간에 유의차가 없었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.38-38
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• 2023

Sorghum (Sorghum bicolor L.) is a promising biomass crop with a high lignocellulose content. This study aimed to select high salt-tolerance sorghum lines for cultivation on reclaimed land. Using 7-day seedlings of the sorghum population consisted of 71 radiation-derived mutants (M₂ to M₆) and 33 genetic resources, survival rate (SR), plant height (PH), root length (RL), fresh weight (FW), and chlorophyll content (CC) were measured for two weeks after 102 mM (0.6%) NaCl treatment. Furthermore, the characteristics of the sorghum population were confirmed using correlation analysis, PCA (principal component analysis), and the FCE (fuzzy comprehensive evaluation) method. Under 102 mM NaCl conditions, SR ranged from 4.9 (IS645-200-6) to 82.4% (KLSo79125-200-1), with an average of 49.9%. PH varied from 7.5 (Mesusu-100-2) to 33.2 cm (DINE-A-MITE-100-2-10), with an average of 20.4 cm. RL ranged from 1.0 (IS645-200-1) to 17.0 cm (30-100-2), with an average of 7.7 cm. FW varied from 0.1 (IS645-200-6) to 4.5 g/plant (DINE-A-MITE-100-2-10), with an average of 2.1 g/plant. CC ranged from 0.9 (DINE-A-MITE-100-2-2) to 3.1 mg/g (IS12937), with an average of 1.7 mg/g. An overall positive correlation, with SR and FW (r = 0.86, P < 0.01), and FW and CC (r = 0.79, P < 0.01), was shown by correlation analysis. Among the five traits, two principal components were extracted by PCA analysis. PC1 was significantly associated with FW, while PC2 was highly involved with RL. To evaluate the salt tolerance level of the sorghum population when an FCE based on trait data was performed, MFV (membership function value) was 0.68. As a result of compiling the MFV of each line, eight lines with MFV > 0.68 were selected. Ultimately, the radiation-derived mutant lines, DINE-A-MITE-100-2-10 and DINE-A-MITE-100-2-12 were selected as salt-tolerant sorghum lines. The results are expected to inform salt-tolerant sorghum breeding programs, and the high salt-tolerance sorghum lines might be advantageous for cultivation on reclaimed land.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.15-15
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• 2021

Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.

• 한국환경복원기술학회지
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• 제27권2호
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• pp.43-53
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• 2024

This study attempted to elucidate the ecological niches and influencing environmental factors of Quercus variabilis and Quercus acutissima, which are representative deciduous broad-leaved trees in Korean forests, taxonomically close and genetically similar, under climate change conditions. Under climate change conditions induced by increased CO₂ and temperature, soil moisture and nutrient environments were manipulated in four gradients. At the end of the growing, plants were harvested to measure growth responses, calculate ecological niches, and compare them with those of the control. Eperimental plants were grown for 180 days in a glass greenhouse designed with four gradients each for soil moisture and nutrient environments under climate change conditions induced by increased CO₂ and temperature. After harvesting, growth responses of leaf traits were measured, ecological niches were calculated, and these were compared with those of the control groups. Furthermore, the responses of the two species' populations were interpreted using principal component analysis(PCA) based on leaf trait measurements. As a result, under climate change conditions, the ecological niche breadth for moisture environment was broader for Quercus variabilis than Quercus acutissima, whereas for the nutrient environment, Quercus acutissima exhibited a broader niche breadth than Quercus variabilis. And the rate of change in ecological niche breadth due to climate change decreased for Quercus variabilis in both moisture and nutrient environments, while for Quercus acutissima, it increased in the moisture environment but decreased in the nutrient environment. Additionally, in terms of group responses, both Quercus variabilis and Quercus acutissima expanded their ecological niches under climate change conditions in both soil moisture and nutrient conditions, with Quercus acutissima exhibiting a broader niche than Quercus variabilis under nutrient conditions. These results indicate that the changes in leaf morphological characteristics and the responses of individuals reflecting them vary not only under climate change conditions but also depending on environmental factors.

• 한국작물학회지
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• 제68권4호
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• pp.343-370
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• 2023

Developing improved wheat varieties is vital for global food security to meet the rising demand for food. Therefore, assessing the genetic diversity across wheat genotypes is crucial. This study examined the diversity of 168 durum wheat and 47 common wheat collections from 54 different countries using twelve agro-morphological parameters. Geumgang, a prominent Korean common wheat variety, was used as a control. Both qualitative and quantitative agronomical characteristics showed wide variations. Most durum wheats were shown to possess dense spikes (90%), while common wheats showed dense (40%) or loose (38%) spikes, with yellowish-white being the dominant spike color. The majority of the accessions were awned regardless of wheat type, yellowish-white being the main awn color. White or red kernels were produced, with white kernels dominating in both common (74%) and durum (79%) wheats. Days to heading (DH) and days to maturity (DM) were in the ranges of 166-215 and 208-250 days, respectively, while the culm length (CL), spike length (SL), and awn length (AL) were in the ranges of 53.67-163, 5.33-18.67, and 0.50-19.00 cm, respectively. Durum wheats possessed the shortest average DH, DM, and SL, while common wheat had the longest CL and AL (p < 0.05). Common wheats also exhibited the highest average one-thousand-kernel weight. Hierarchical cluster analysis, aided by principal component analysis, grouped the population into seven clusters with significant differences in their quantitative variables (p < 0.05). In conclusion, this research revealed diversity among common and durum wheat genotypes. Notably, 26 durum wheat and 17 common wheat accessions outperformed the control, offering the potential for developing early-maturing, high-yielding, and lodging-resistant wheat varieties.