• 제목/요약/키워드: general forms

검색결과 862건 처리시간 0.033초

Molecular Cloning and Analysis of the Gene for P-450 Hydroxylase from Pseudonocardia autotrophica IFO 12743

  • Kim, Jung-Mee;Younmie Jin;Hyun, Chang-Gu;Kim, Jong-Hee;Lee, Hong-Sub;Kang, Dae-Kyung;Kang, Dae-Jung;Kim, Tae-Yong;Suh, Joo-Won
    • Journal of Microbiology
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    • 제40권3호
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    • pp.211-218
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    • 2002
  • A 4.8-kb DNA fragment encoding the P-450 type hydroxylase and ferredoxin genes was cloned from Pseudonocardia autotrophica IFO 12743 that can convert vitamin D$\_$3/ into its hydroxylated active forms. In order to isolate the P-450 gene cluster in this organism, we designed PCR primers on the basis of the regions of an oxygen binding site and a heme ligand pocket that are general characteristics of the P-450 hydroxylase. Sequencing analysis of the BamHI fragment revealed the presence of four complete and one incomplete ORFs, named PauA, PauB, PauC, and PauD, respectively. As a result of computer-based analyses, PauA and PauB have homology with enoyl-CoA hydratase from several organisms and the positive regulators belonging to the tetR family, respectively. PauC and PauD show similarity with SuaB/C proteins and ferredoxins, respectively, which are composed of P-450 monooxygenase systems for metabolizing two sulfonylurea herbicides in Streptomyces griseolus PauC shows the highest similarity with another CytP-450$\_$Sca2/ protein that is responsible for production of a specific HMG-CoA reductase inhibitor, pravastatin, in S. carbophilus. Cultures of Steptomyces lividans transformant, containing the P-450 gene cluster on the pWHM3 plasmid, was unable to convert vitamin D$\_$3/ to its hydroxylated forms.

생태복원재료 관련 국내 실정법의 속성 분석 (An Analysis on the Properties of the Domestic Laws Connected with Ecological Restoration Materials)

  • 신익순
    • 한국환경복원기술학회지
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    • 제7권1호
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    • pp.85-96
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    • 2004
  • The purpose of this study is to present the legal object in the related legislation taking the present condition of the domestic laws and the leading cases connected with ecological restoration materials and analyzing their properties according to the analytic standards which are legal class and ecological restoration material items, the present condition by the field of ecological restoration business, interrelationship between the law and the leading cases, comparison of the domestic laws with the foreign regulations. The results of this study are as follows; 1) Detailed enforcement regulation(36.5% of totals), enforcement regulation(32.4%) and law(31.1%) as legal class of the domestic legislation relating to ecological restoration materials are in the order of frequency that shows the little strong frequency at low-ranking class. By items, the number of legislation relating to planting material holds about majority, next to it, stone material, soil material and wood material are in the order of frequency. 2) By the field of ecological restoration business, legislation relating to administration forms the highest frequency(36.3% of totals), next to it, material properties(23.4%), plan design(13.0%) are in the order of frequency. 3) For the number of the leading cases by items of ecological restoration materials, those are, for the most part, them relating to planting material(93.8% of totals). The number of legislation relating to planting material forms the highest frequency at laws and the leading cases in common. 4) The domestic legislation connected with ecological restoration materials is mainly to be in legal class of the positive law, on the contrary, a foreign country has legislation widely consisted of laws, ordinances and other general regulations. Some foreign country legislated the topsoil conservation act, but not to domestic. The result of this study will be applied to legislature and court as reference materials, and to the public and public officer as a means of an understanding of ecological restoration materials.

Rhizopus oryzae로부터 정제한 두가지형의 Glucoamylase의 효소적(酵素的) 특성(特性) (Enzymatic Characteristics of Two Forms of the Purified Glucoamylase from Rhizopus oryzae)

  • 허원영;정만재
    • 한국식품과학회지
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    • 제16권4호
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    • pp.392-397
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    • 1984
  • Rhiz. oryzae가 생산(生産) 정제효소(精製酵素) glucoamylase I (G I) 과 glucoamylase II (G II) 의 gel filtration에 의(依)하여 추정(推定)된 분자량(分子量)은 120,000, 127,000이었다. glucoamylase I 과 II 의 등전점(等電点)은 각각(各各) pH 7.25. pH 7.75 이었고 반응(反應) 최적온도(最適溫度)는 다같이 $50^{\circ}C$, 안정온도범위(安定溫度範圍) 는 다같이 pH5.0부근(附近)이었으며 안정(安定)pH범위(範圍)는 각각(各各) $pH3.5{\sim}8.0$, $pH4.5{\sim}8.0$이었다. 가용성전분(可溶性澱粉)에 대(對)한 Michaelis 상수는 glucoamylase I 이 4.545mg/ml, glucoamylase II 가 5.560mg/ml이었다. glucoamylase I 은 $Hg^{++}$, $Pb^{++}$, p-CMB, IAA에 의하여, glucoamylase II는 $Hg^{++}$, $Mn^{++}$, p-CMB, IAA에 의하여 저해(沮害)되었다.

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Experimental verification of the linear and non-linear versions of a panel code

  • Grigoropoulos, G.J.;Katsikis, C.;Chalkias, D.S.
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제3권1호
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    • pp.27-36
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    • 2011
  • In the proposed paper numerical calculations are carried out using two versions of a three-dimensional, timedomain panel method developed by the group of Prof. P. Sclavounos at MIT, i.e. the linear code SWAN2, enabling optionally the use of the instantaneous non-linear Froude-Krylov and hydrostatic forces and the fully non-linear SWAN4. The analytical results are compared with experimental results for three hull forms with increasing geometrical complexity, the Series 60, a reefer vessel with stern bulb and a modern fast ROPAX hull form with hollow bottom in the stern region. The details of the geometrical modeling of the hull forms are discussed. In addition, since SWAN4 does not support transom sterns, only the two versions of SWAN2 were evaluated over experimental results for the parent hull form of the NTUA double-chine, wide-transom, high-speed monohull series. The effect of speed on the numerical predictions was investigated. It is concluded that both versions of SWAN2 the linear and the one with the non-linear Froude-Krylov and hydrostatic forces provide a more robust tool for prediction of the dynamic response of the vessels than the non-linear SWAN4 code. In general, their results are close to what was expected on the basis of experience. Furthermore, the use of the option of non-linear Froude-Krylov and hydrostatic forces is beneficial for the accuracy of the predictions. The content of the paper is based on the Diploma thesis of the second author, supervised by the first one and further refined by the third one.

방화 발생에 영향을 미치는 요인에 관한 연구 (A Study on the Factors Affecting the Arson)

  • 김영철;박우성;이수경
    • 한국화재소방학회논문지
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    • 제28권2호
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    • pp.69-75
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    • 2014
  • 본 연구에서는 방화발생에 영향을 미치는 요인을 도출하기 위하여 발생건수를 종속변수로 하고 경제 인구 사회적 요인을 독립변수로 하는 다중회귀분석을 실시하였다. 다중회귀분석은 선형함수, 준로그함수, 역준로그함수, 이중로그함수 4가지 함수형태에 대해 적용하였으며, 각 단계별로 변수의 선택과 제외를 고려하는 단계적선택 방식을 적용하였다. 다중공선성 문제와 자기상관 문제를 해결하기 위하여 분산확대지수(VIF)와 Durbin-Watson 계수 이용하였으며, 4가지 함수모형에 대하여 수정된 R 제곱(설명력) 값이 0.935 (93.5%)로 가장 값이 높고 통계적으로 유의한 선형함수모형을 최적의 모형으로 결정하고 모형에 대한 해석을 진행하였다. 선형함수모형 결과 방화발생에 영향을 미치는 요인은 범죄발생건수(0.829), 일반이혼율(0.151), 재정자주도(0.149), 소비자물가상승률(0.099) 순으로 도출되었다.

미간벌(未間伐) 인공림(人工林)에서 잔존림목(殘存林木) 추정(推定) 함수(函數)의 유도(誘導)와 적용(適用) (Derivation and Application of Survival Functions for Unthinned Forest Plantation)

  • 이봉일;정주상;권순덕
    • 한국산림과학회지
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    • 제88권3호
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    • pp.320-326
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    • 1999
  • 연구에서는 잔존목의 본수에 대한 수식형태 및 고사율 추정 미분함수형태를 가정하여 인공림에 대한 잔존목을 추정하기 위한 15개의 함수들을 적분 및 지수함수형태로 유도하였다. 또한 이 모델들을 간벌이 되지 않은 스트로부스 잣나무 인공림의 반복측정 자료를 이용하여 모델의 적용성을 검토하였다. 그 결과 $t^{\beta}$와 같이 임령의 지수형태를 포함하는 3개의 함수들이 시간에 따른 자연 간벌효과를 설명하는데 상대적으로 유효한 것으로 나타났다. 한편 지위가 자연 간벌에 미치는 효과를 분석하기 위하여 낙엽송 임분의 잔존목 추정을 위한 함수를 유도하였다. 그 결과 $N_{min}$이 지위지수와 부의 상관관계를 가지는 것으로 나타났고, 초기 임분밀도를 같은 값으로 가정한 경우에도 잔존목 추정함수의 곡선이 지위지수별로 달라짐을 알 수 있었다.

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수학 영재들을 4차원 도형에 대한 탐구로 안내하는 사례 연구 (A Case Study on Guiding the Mathematically Gifted Students to Investigating on the 4-Dimensional Figures)

  • 송상헌
    • 영재교육연구
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    • 제15권1호
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    • pp.85-102
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    • 2005
  • 이 연구는 경기과학고등학교 1학년 학생 5명을 대상으로 사사연구를 진행하면서 학생들이 탐구한 수학적인 내용에 대한 분석과 그 결과가 나오기까지 멘토링을 하는 지도교수의 역할을 설명하고 있다. 학생들이 탐구한 수학적인 내용은 4차원 도형의 모양과 그 도형들에 나타나는 수학적인 성질이다. 지도교수는 연구에 익숙하지 않은 학생들을 위하여 수학자 피코크가 제안했던 '형식불역의 원리'를 모델로 삼도록 했고, 지도교수는 학생들의 창조적인 산출물 생산을 격려하기 위해 수학교육학자 프로이덴탈의 '안내된 재발명의 방법'을 사용하였다. 학생들은 지도교수의 안내에 의한 (재)발명의 원리에 따라 기존에 이미 알고 있던 수학적 성질을 고차원 도형에 적용시키면서 확장, 일반화시켜나갔는데, 여기에는 '형식불역의 원리'라는 틀이 매우 유용하게 작용하였다. 지도교사는 학생들에게 3차원 도형을 2차원에 표현하는 겨냥도, 전개도, 평면그래프를 응용하여 4차원을 3차원과 2차원에 표현하는 방식을 탐구하도록 하였다. 이 과정에서 학생들은 이미 알려진 파스칼의 삼각형과 이항정리, 오일러 정리, 하세의 다이어그램 등을 4차원 이상의 도형을 탐구할 때에도 적용할 수 있음을 확인하였다. 그리고 몇 가지의 추측과 후속 연구 과제를 제안하였다. 학생들의 산출물들은 형식불역의 원리와 안내된 재발명의 방법의 결과물인 것이다. 이 연구는 사사연구의 과정에 도움이 될 수 있는 3가지의 제안과 그 실 예를 담고 있다.

외국인을 위한 한글맞춤법 시안 연구 (A Study on Hangeul Orthography Guidelines for Foreigners)

  • 한재영
    • 한국어교육
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    • 제28권4호
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    • pp.273-296
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    • 2017
  • This study focuses on a review of Hangeul orthography guidelines in Korean language regulations. It is indispensable to revise the guidelines thoroughly because it has been more than 80 years since a unified plan of Korean orthography was established in 1933, which the current orthography is based on. Also, it has been approximately 30 years since 1989, when the current guidelines were issued and promulgated. The viewpoint towards this review reflects the requirements by education fields of Korean as a foreign language and modern Korean users. Hangeul orthography consists of six clauses, along with an appendix regarding punctuation marks: 1) general rules, 2) consonants and vowels, 3) related to sounds, 4) about forms, 5) spacing between words, and 6) miscellaneous. This paper examined individual clauses and specific usages of the clauses, in terms of Korean as a foreign language. Based on the review, this paper suggests the following tasks in order to establish a draft of Hangeul orthography for foreigners. A. Among the individual clauses, some clauses that embody vocabulary education aspects should be addressed in a Korean dictionary, and deleted in Hangeul orthography guidelines. B. The clauses of Hangeul orthography guidelines should be edited for revision and substitution where necessary. C. The usage of individual clauses should be replaced with more appropriate examples aligned with everyday conversation. D. In order to establish 'Hangeul orthography for foreigners', linguists should continuously review several chapters and the appendix of Hangeul orthography, such as components about forms, spacing between words, miscellaneous, and punctuation marks. The purpose of this review is to pursue the simplicity of Hangeul orthography guidelines and the practicality in terms of reflecting more realistic examples. This review contributes to facilitate Korean language usage not only for non-native learners, but also native users.

백팩 착용 유무 및 형태에 따른 건강한 성인의 몸통과 하지의 근 활성도 비교분석의 융합연구 (Convergence of Comparison of Muscle Activities of Healthy Adults' trunk and Lower Extremities according to wearing/not-wearing and forms of backpack)

  • 김경훈;장상훈
    • 한국융합학회논문지
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    • 제10권11호
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    • pp.225-232
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    • 2019
  • 본 연구의 목적은 건강한 대학생을 대상으로 백팩 착용 유무 및 형태에 따른 몸통 하지 근 활성도 변인들의 차이점을 규명하는데 그 목적을 두고 있다. 26명의 건강한 성인이 연구에 참여하였다. 백팩의 무게 10%로 부하된 상태에서, 백팩을 착용하지 않은 상태, 일반적인 백팩을 착용한 상태, 모멘트 팔을 감소시키도록 고안된 백팩을 착용한 상태에서 몸통과 하지의 근활성도를 측정하였다. 배빗근육, 넙다리네갈래근, 앞정강근, 장딴지근의 근 활성도 분석을 위해 무선근전도 시스템을 사용하였다. 모멘트 팔을 감소시키도록 고안된 백팩을 착용한 상태에서 배빗근육, 넙다리네갈래근 그리고 장딴지근의 보행 구간에서 RVC에 변화가 통계학적으로 유의한 차이를 보였다. 그러므로, 백팩을 주로 사용하는 성인들에게 가방끈 조절을 적용하는 방법은 몸통과 다리근육의 활성에 긍정적인 측면을 제공한다는 것을 나타내며, 추후 가방의 디자인과 착용방법에 대한 지속적인 가방융합개발이 필요할 것이다.

Improving the Professional Competence of a Specialist in Poland by Implementing Multimedia Technologies

  • Kravchenko, Tetiana;Varga, Lesia;Lypchanko-Kovachyk, Oksana;Chinchoy, Alexander;Yevtushenko, Nataliia;Syladii, Ivan;Kuchai, Oleksandr
    • International Journal of Computer Science & Network Security
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    • 제22권9호
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    • pp.51-58
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    • 2022
  • The article emphasizes the features of the modern education system in Poland, reveals the peculiarities of improving the professional competence of a specialist in Poland through the implementation of multimedia technologies. Various forms of innovations implemented in improving the professional competence of a specialist are listed: improvement (rationalization), modernization, innovation. The forms of professional improvement through the introduction of computer technologies in general and multimedia technologies, in particular, primarily include various professional courses, qualification, preparatory, methodological conferences, seminars, postgraduate studies, foreign and state internships. At the same time, the main direction is self-education. The subject of professional improvement in the application of computer technologies by specialists is the updating of existing knowledge, exchange of professional experience, planning, as well as discussion of innovative works in which specialists participate. Professional growth of specialists can occur both during work and in higher education institutions during their studies. Modernization of computer technologies, especially multimedia ones, is a necessary condition for the functioning of specialists in modern society, since specialists are at the center of the educational process, during the improvement of professional competence. The main functions of the educational process necessary for improving the professional competence of specialists through the implementation of multimedia technologies are revealed. These functions not only contribute to the professional improvement of specialists, but also affect their solutions and optimize the maintenance of contacts between specialists. The importance of creating conditions that are consistent with the modern needs of innovative education is emphasized.