• Title/Summary/Keyword: general forms

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Molecular Cloning and Analysis of the Gene for P-450 Hydroxylase from Pseudonocardia autotrophica IFO 12743

  • Kim, Jung-Mee;Younmie Jin;Hyun, Chang-Gu;Kim, Jong-Hee;Lee, Hong-Sub;Kang, Dae-Kyung;Kang, Dae-Jung;Kim, Tae-Yong;Suh, Joo-Won
    • Journal of Microbiology
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    • v.40 no.3
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    • pp.211-218
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    • 2002
  • A 4.8-kb DNA fragment encoding the P-450 type hydroxylase and ferredoxin genes was cloned from Pseudonocardia autotrophica IFO 12743 that can convert vitamin D$\_$3/ into its hydroxylated active forms. In order to isolate the P-450 gene cluster in this organism, we designed PCR primers on the basis of the regions of an oxygen binding site and a heme ligand pocket that are general characteristics of the P-450 hydroxylase. Sequencing analysis of the BamHI fragment revealed the presence of four complete and one incomplete ORFs, named PauA, PauB, PauC, and PauD, respectively. As a result of computer-based analyses, PauA and PauB have homology with enoyl-CoA hydratase from several organisms and the positive regulators belonging to the tetR family, respectively. PauC and PauD show similarity with SuaB/C proteins and ferredoxins, respectively, which are composed of P-450 monooxygenase systems for metabolizing two sulfonylurea herbicides in Streptomyces griseolus PauC shows the highest similarity with another CytP-450$\_$Sca2/ protein that is responsible for production of a specific HMG-CoA reductase inhibitor, pravastatin, in S. carbophilus. Cultures of Steptomyces lividans transformant, containing the P-450 gene cluster on the pWHM3 plasmid, was unable to convert vitamin D$\_$3/ to its hydroxylated forms.

An Analysis on the Properties of the Domestic Laws Connected with Ecological Restoration Materials (생태복원재료 관련 국내 실정법의 속성 분석)

  • Shin, Ick-Soon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.1
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    • pp.85-96
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    • 2004
  • The purpose of this study is to present the legal object in the related legislation taking the present condition of the domestic laws and the leading cases connected with ecological restoration materials and analyzing their properties according to the analytic standards which are legal class and ecological restoration material items, the present condition by the field of ecological restoration business, interrelationship between the law and the leading cases, comparison of the domestic laws with the foreign regulations. The results of this study are as follows; 1) Detailed enforcement regulation(36.5% of totals), enforcement regulation(32.4%) and law(31.1%) as legal class of the domestic legislation relating to ecological restoration materials are in the order of frequency that shows the little strong frequency at low-ranking class. By items, the number of legislation relating to planting material holds about majority, next to it, stone material, soil material and wood material are in the order of frequency. 2) By the field of ecological restoration business, legislation relating to administration forms the highest frequency(36.3% of totals), next to it, material properties(23.4%), plan design(13.0%) are in the order of frequency. 3) For the number of the leading cases by items of ecological restoration materials, those are, for the most part, them relating to planting material(93.8% of totals). The number of legislation relating to planting material forms the highest frequency at laws and the leading cases in common. 4) The domestic legislation connected with ecological restoration materials is mainly to be in legal class of the positive law, on the contrary, a foreign country has legislation widely consisted of laws, ordinances and other general regulations. Some foreign country legislated the topsoil conservation act, but not to domestic. The result of this study will be applied to legislature and court as reference materials, and to the public and public officer as a means of an understanding of ecological restoration materials.

Enzymatic Characteristics of Two Forms of the Purified Glucoamylase from Rhizopus oryzae (Rhizopus oryzae로부터 정제한 두가지형의 Glucoamylase의 효소적(酵素的) 특성(特性))

  • Hou, Won-Nyong;Chung, Man-Jae
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.392-397
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    • 1984
  • These experiments were conducted to investigate general enzymatic characteristics of two forms(glucoamylase I and glucoamylase II) of the purified glucoamylase produced by Rhizopus oryzae. Molecular weights of glucoamylase I and glucoamylase II estimated by Sephadex G-100gel filtration, were approximately 101,000 and 115,000, respectively, and those estimated by SDS-polyacrylamide gel electrophoresis being 120,000 and 127,000, respectively. Isoelectric points of the above enzyme were pH 7.25 and pH 7.75. The optimum temperature was $50^{\circ}C$ and the enzyme was stable below $45^{\circ}C$. Optimum pH of both glucoamylase I and glucoamylase II was about pH 5.0. The stable pH range of them were pH 3.5-8.0 and 4.5-8.0, respectively. Michaelis constants of glucoamylase I and glucoamylase II toward souluble starch were 4.545 mg/ml and 5.560 mg/ml, respectively. $Hg^{++}$, $Pb^{++}$, p-CMB and IAA were inhibitors of glucoamylase I and $Hg^{++}$, $Mn^{++}$, p-CMB and IAA were inhibitors of glucoamylase II.

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Experimental verification of the linear and non-linear versions of a panel code

  • Grigoropoulos, G.J.;Katsikis, C.;Chalkias, D.S.
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.3 no.1
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    • pp.27-36
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    • 2011
  • In the proposed paper numerical calculations are carried out using two versions of a three-dimensional, timedomain panel method developed by the group of Prof. P. Sclavounos at MIT, i.e. the linear code SWAN2, enabling optionally the use of the instantaneous non-linear Froude-Krylov and hydrostatic forces and the fully non-linear SWAN4. The analytical results are compared with experimental results for three hull forms with increasing geometrical complexity, the Series 60, a reefer vessel with stern bulb and a modern fast ROPAX hull form with hollow bottom in the stern region. The details of the geometrical modeling of the hull forms are discussed. In addition, since SWAN4 does not support transom sterns, only the two versions of SWAN2 were evaluated over experimental results for the parent hull form of the NTUA double-chine, wide-transom, high-speed monohull series. The effect of speed on the numerical predictions was investigated. It is concluded that both versions of SWAN2 the linear and the one with the non-linear Froude-Krylov and hydrostatic forces provide a more robust tool for prediction of the dynamic response of the vessels than the non-linear SWAN4 code. In general, their results are close to what was expected on the basis of experience. Furthermore, the use of the option of non-linear Froude-Krylov and hydrostatic forces is beneficial for the accuracy of the predictions. The content of the paper is based on the Diploma thesis of the second author, supervised by the first one and further refined by the third one.

A Study on the Factors Affecting the Arson (방화 발생에 영향을 미치는 요인에 관한 연구)

  • Kim, Young-Chul;Bak, Woo-Sung;Lee, Su-Kyung
    • Fire Science and Engineering
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    • v.28 no.2
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    • pp.69-75
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    • 2014
  • This study derives the factors which affect the occurrence of arson from statistical data (population, economic, and social factors) by multiple regression analysis. Multiple regression analysis applies to 4 forms of functions, linear functions, semi-log functions, inverse log functions, and dual log functions. Also analysis respectively functions by using the stepwise progress which considered selection and deletion of the independent variable factors by each steps. In order to solve a problem of multiple regression analysis, autocorrelation and multicollinearity, Variance Inflation Factor (VIF) and the Durbin-Watson coefficient were considered. Through the analysis, the optimal model was determined by adjusted Rsquared which means statistical significance used determination, Adjusted R-squared of linear function is scored 0.935 (93.5%), the highest of the 4 forms of function, and so linear function is the optimal model in this study. Then interpretation to the optimal model is conducted. As a result of the analysis, the factors affecting the arson were resulted in lines, the incidence of crime (0.829), the general divorce rate (0.151), the financial autonomy rate (0.149), and the consumer price index (0.099).

Derivation and Application of Survival Functions for Unthinned Forest Plantation (미간벌(未間伐) 인공림(人工林)에서 잔존림목(殘存林木) 추정(推定) 함수(函數)의 유도(誘導)와 적용(適用))

  • Li, Fengri;Chung, Joosang;Kwon, Soonduk
    • Journal of Korean Society of Forest Science
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    • v.88 no.3
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    • pp.320-326
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    • 1999
  • In this study, 15 survival functions in integral and difference forms for forest plantation were derived based on assumptions for the number of surviving trees and the differential forms of the mortality rate model. Then, performance of the models was evaluated by fitting to remeasurement data of unthinned white pine(Pinus strobes) forest plantation. As a result, three equations associated with a power function of age, $t^{\beta}$, are somewhat more suitable for describing the effect of self-thinning over time. On the other hand, a general survival function for Japanese larch(Larix leptolepts) forest plantation was derived in order to exam the effect of site quality on self-thinning procedures. The results indicate that the $N_{min}$ is negatively correlated with site index and, even though the same initial stand density was assumed, the survival function curves differ in shapes associated with site index values.

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A Case Study on Guiding the Mathematically Gifted Students to Investigating on the 4-Dimensional Figures (수학 영재들을 4차원 도형에 대한 탐구로 안내하는 사례 연구)

  • Song, Sang-Hun
    • Journal of Gifted/Talented Education
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    • v.15 no.1
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    • pp.85-102
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    • 2005
  • Some properties on the mathematical hyper-dimensional figures by 'the principle of the permanence of equivalent forms' was investigated. It was supposed that there are 2 conjectures on the making n-dimensional figures : simplex (a pyramid type) and a hypercube(prism type). The figures which were made by the 2 conjectures all satisfied the sufficient condition to show the general Euler's Theorem(the Euler's Characteristics). Especially, the patterns on the numbers of the components of the simplex and hypercube are fitted to Binomial Theorem and Pascal's Triangle. It was also found that the prism type is a good shape to expand the Hasse's Diagram. 5 mathematically gifted high school students were mentored on the investigation of the hyper-dimensional figure by 'the principle of the permanence of equivalent forms'. Research products and ideas students have produced are shown and the 'guided re-invention method' used for mentoring are explained.

A Study on Hangeul Orthography Guidelines for Foreigners (외국인을 위한 한글맞춤법 시안 연구)

  • Han, Jae young
    • Journal of Korean language education
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    • v.28 no.4
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    • pp.273-296
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    • 2017
  • This study focuses on a review of Hangeul orthography guidelines in Korean language regulations. It is indispensable to revise the guidelines thoroughly because it has been more than 80 years since a unified plan of Korean orthography was established in 1933, which the current orthography is based on. Also, it has been approximately 30 years since 1989, when the current guidelines were issued and promulgated. The viewpoint towards this review reflects the requirements by education fields of Korean as a foreign language and modern Korean users. Hangeul orthography consists of six clauses, along with an appendix regarding punctuation marks: 1) general rules, 2) consonants and vowels, 3) related to sounds, 4) about forms, 5) spacing between words, and 6) miscellaneous. This paper examined individual clauses and specific usages of the clauses, in terms of Korean as a foreign language. Based on the review, this paper suggests the following tasks in order to establish a draft of Hangeul orthography for foreigners. A. Among the individual clauses, some clauses that embody vocabulary education aspects should be addressed in a Korean dictionary, and deleted in Hangeul orthography guidelines. B. The clauses of Hangeul orthography guidelines should be edited for revision and substitution where necessary. C. The usage of individual clauses should be replaced with more appropriate examples aligned with everyday conversation. D. In order to establish 'Hangeul orthography for foreigners', linguists should continuously review several chapters and the appendix of Hangeul orthography, such as components about forms, spacing between words, miscellaneous, and punctuation marks. The purpose of this review is to pursue the simplicity of Hangeul orthography guidelines and the practicality in terms of reflecting more realistic examples. This review contributes to facilitate Korean language usage not only for non-native learners, but also native users.

Convergence of Comparison of Muscle Activities of Healthy Adults' trunk and Lower Extremities according to wearing/not-wearing and forms of backpack (백팩 착용 유무 및 형태에 따른 건강한 성인의 몸통과 하지의 근 활성도 비교분석의 융합연구)

  • Kim, Kyung-Hun;Jang, Sang-Hun
    • Journal of the Korea Convergence Society
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    • v.10 no.11
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    • pp.225-232
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    • 2019
  • This study aims to compare muscle activities of trunk and lower extremities according to wearing/not-wearing and forms of backpack. Twenti-six healthy adults were participated in the study. In the backpack with 10% body weight, trunk and lower extremities were measured during carrying the no backpack state(NBs), general backpack state(GBs), a decrease moment arm backpack state(DMBs). The muscle activity in a fore muscles(Obilique, Quadriceps, Tibialis anterior, Gastrocnemius) were analyzed with using wireless electromyogram measuring system. DMBs showed significant improvemet in obilique, quadriceps and gastrocnemius muscle RVC.

Improving the Professional Competence of a Specialist in Poland by Implementing Multimedia Technologies

  • Kravchenko, Tetiana;Varga, Lesia;Lypchanko-Kovachyk, Oksana;Chinchoy, Alexander;Yevtushenko, Nataliia;Syladii, Ivan;Kuchai, Oleksandr
    • International Journal of Computer Science & Network Security
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    • v.22 no.9
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    • pp.51-58
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    • 2022
  • The article emphasizes the features of the modern education system in Poland, reveals the peculiarities of improving the professional competence of a specialist in Poland through the implementation of multimedia technologies. Various forms of innovations implemented in improving the professional competence of a specialist are listed: improvement (rationalization), modernization, innovation. The forms of professional improvement through the introduction of computer technologies in general and multimedia technologies, in particular, primarily include various professional courses, qualification, preparatory, methodological conferences, seminars, postgraduate studies, foreign and state internships. At the same time, the main direction is self-education. The subject of professional improvement in the application of computer technologies by specialists is the updating of existing knowledge, exchange of professional experience, planning, as well as discussion of innovative works in which specialists participate. Professional growth of specialists can occur both during work and in higher education institutions during their studies. Modernization of computer technologies, especially multimedia ones, is a necessary condition for the functioning of specialists in modern society, since specialists are at the center of the educational process, during the improvement of professional competence. The main functions of the educational process necessary for improving the professional competence of specialists through the implementation of multimedia technologies are revealed. These functions not only contribute to the professional improvement of specialists, but also affect their solutions and optimize the maintenance of contacts between specialists. The importance of creating conditions that are consistent with the modern needs of innovative education is emphasized.