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Endophytic Yeasts Colonize Roots of Ulmus parvifolia Jacq. and Quercus salicina Blume

  • Kim, Jong-Shik;Kim, Dae-Shin
    • Korean Journal of Environmental Agriculture
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    • v.36 no.2
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    • pp.135-139
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    • 2017
  • BACKGROUND: Identification and characterization of endophytic yeasts inhabiting the roots of Ulmus parvifolia Jacq. and Quercus salicina Blume require biotechnological and culture-based techniques. METHODS AND RESULTS: Homogenized U. parvifolia and Q. salicina root samples were spread onto four types of agar medium containing ancgtibiotics, L-sorbose, and Triton X-100. In total, 25 yeast strains were isolated and subjected to phylogenetic analysis based on their internal transcribed spacer region sequences. The results revealed that the yeast genera Cyberlindnera (12 isolates) and Cryptococcus (1 isolate) were associated with roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) were associated with roots of Q. salicina. Additionally, a Kluyveromyces isolate produced a detectable level of bioethanol. The yeast strains reported herein may be used in industrial production of biosurfactants and bioethanol. CONCLUSION: Our findings revealed that the endophytic yeast genera Cyberlindnera and Cryptococcus predominated in roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) predominated in roots of Q. salicina. Additionally, Kluyveromyces isolates produced a detectable level of bioethanol.

Drosophilid Fauna of Six Regions Near the Demilitarized Zone in Korea (韓國非武裝地帶隣近 6個地域의 초파리 分布相)

  • Kang, Yung-Sun;Moon, Kwang-Woong
    • The Korean Journal of Zoology
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    • v.11 no.2
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    • pp.65-68
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    • 1968
  • Collections of the drosophilid flies were made at 6 regions near the dimilitarized znoe form October 1966 to June 1968, with particular attention to their habitats and distribution. A total of 5022 flies representing 34 species belonging to 7 genera were obtained mostly by the traps baited with fermenting apples and banana, partially by the aid of net. The data accumulated for about 21 months are summarized in this paper. Drosophila auraria and D. angularis were found to be most common species showing the most extensive distribution. In eastern part, a total of 1552 individuals(30.90%) representing 27 species, 7 genera were sampled. In central part, a total of 2658 flies (52.93%) yielded 29 species, 6 genera. In western part, a total of 812 specimens(16.17%) which belong to 16 species representing 3 genera were collected. Most specimens belonging to the genus Scaptomyza have been found on grasses.

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Host Plants of Popillia quadriguttata (Coleoptera: Scarabaeidae) (녹색콩풍뎅이(Popillia quadriguttata)의 기주식물)

  • 이동운;추호렬;정재민;이상명;사공영보
    • Korean journal of applied entomology
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    • v.41 no.1
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    • pp.15-19
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    • 2002
  • Host plants of Popillia quadriguttata (Fabricius) were investigated in golf courses, Fifteen plant species of 15 genera in 13 families were confirmed as host plants of P. quadriguttata. Thus, total number of host plants of P. quadriguttata was 26 species of 25 genera in 18 families including 11 recorded species in literature. In addition, P. quadriguttata adults fed on 30 plant species of 25 genera in 19 families out of the 69 plant species of 53 genera in 33 families supplied as food in laboratory, P. quadriguttata adults preferred Wistaria floribunda in golf courses but Tilia mandshuric, Platanus orientalis, Diospyros lotus, Punica granatum and Chionanthus retusa in laboratory.

Fungi Isolated from Pine Wood Nematode, Its Vector Japanese Pine Sawyer, and the Nematode-Infected Japanese Black Pine Wood in Korea

  • Hyun, Min-Woo;Kim, Ji-Hye;Suh, Dong-Yeon;Lee, Seung-Kyu;Kim, Seong-Hwan
    • Mycobiology
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    • v.35 no.3
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    • pp.159-161
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    • 2007
  • Pine tree death caused by pine wood nematode(PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle(JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. Frpm JPS adult beetles, nine genera of Aspergillus, Gibberalla, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidio-mycetes were found. Ten genera from PWN-infected weed were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectria, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

Description of 42 unrecorded bacterial species in Korea, belonging to the class Alphaproteobacteria

  • Liu, Qingmei;Kim, Seung-Bum;Yoon, Jung-Hoon;Joh, Kiseong;Seong, Chi-Nam;Jeon, Che-Ok;Kim, Wonyong;Kim, Myung Kyum;Im, Wan-Taek
    • Journal of Species Research
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    • v.8 no.4
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    • pp.351-364
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    • 2019
  • Here we describe indigenous prokaryotic species in Korea, a total of 42 bacterial strains affiliated to the class Alphaproteobacteria isolated from various environmental samples: fermented vinegar, sea water, beach sand, fresh water, salt flats, moss, algae, activated sludge, and soil. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to predefined bacterial species. There is no official report that these 42 species included in Alphaproteobacteria in Korea: 15 species of 6 genera in the order Rhodospirillales, 12 species of 10 genera in the order Rhizobiales, 10 species of 8 genera in the order Rhodobacterales, 4 species of 4 genera in the order Sphingomonadales and 1 species of 1 genus in the order Caulobacterales. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

Geographical Isolation and Root-Associated Fungi in the Marine Terrains: A Step Toward Establishing a Strategy for Acquiring Unique Microbial Resources

  • Park, Jong Myong;Hong, Ji Won;Lee, Woong;Lee, Byoung-Hee;You, Young-Hyun
    • Mycobiology
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    • v.49 no.3
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    • pp.235-248
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    • 2021
  • This study aimed to understand whether the geo-ecological segregation of native plant species affects the root-associated fungal community. Rhizoplane (RP) and rhizosphere (RS) fungal microbiota of Sedum takesimense native to three geographically segregated coastal regions (volcanic ocean islands) were analyzed using culture-independent methods: 568,507 quality sequences, 1399 operational taxonomic units, five phyla, and 181 genera were obtained. Across all regions, significant differences in the phyla distribution and ratio were confirmed. The Chao's richness value was greater for RS than for RP, and this variance coincided with the number of genera. In contrast, the dominance of specific genera in the RS (Simpson value) was lower than the RP at all sites. The taxonomic identity of most fungal species (95%) closely interacting with the common host plant was different. Meanwhile, a considerable number of RP only residing fungal genera were thought to have close interdependency on their host halophyte. Among these, Metarhizium was the sole genus common to all sites. These suggest that the relationship between potential symbiotic fungi and their host halophyte species evolved with a regional dependency, in the same halophyte species, and of the same natural habitat (volcanic islands); further, the fungal community differenced in distinct geographical regions. Importantly, geographical segregation should be accounted for in national culture collections, based on taxonomical uniqueness.

Nineteen new records of plant species including two new genera recorded from the Bhutan Himalayas

  • DORJI, Rinchen;PHUENTSHO, Phuentsho;DORJI, Kencho;TSHEWANG, Sangay;WANGDI, Phuntsho;TOBGAY, Kezang;GYELTSHEN, Nima;GYELTSHEN, Choki
    • Korean Journal of Plant Taxonomy
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    • v.52 no.3
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    • pp.173-183
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    • 2022
  • Through the collection of herbarium specimens in Bhutan over the years, new plant species are discovered nearly annually. Thus, this paper reports two new genera and 19 new records of flowering plant species from Bhutan. The new genera include Eurycorymbus of the family Sapindaceae and Homalium of the family Salicaceae. The new records of plant species are Eranthemum erythrochilum (Acanthaceae), Hemidesmus indicus (Apocynaceae), Ilex umbellulata (Aquifoliaceae), Canarium strictum (Burseraceae), Ehretia acuminate (Boraginaceae), Vaccinium sikkimense (Ericaceae), Nothapodytes foetida (Icacinaceae), Machilus edulis (Lauraceae), Grewia asiatica (Malvaceae), Hibiscus fragrans (Malvaceae), Cipadessa baccifera (Meliaceae), Baccaurea javanica (Phyllanthaceae), Canthiumera glabra (Rubiaceae), Homalium napaulense (Salicaceae), Eurycorymbus cavaleriei (Sapindaceae), Acmella radicans (Asteraceae), Silene latifolia (Caryophyllaceae), Cleome rutidosperma (Cleomaceae), and Cuphea carthagenensis (Lythraceae). Morphological determinations of the genera and species were carried out at the National Herbarium (THIM) of the National Biodiversity Centre of Bhutan. Brief descriptions of the species, phenology, and photo plates are provided in this annotated checklist.

A List of Important Species and Distribution of Marine Phytoplankton in Korea (국내 해양식물플랑크톤의 주요종과 분포에 대한 조사)

  • 문성기;최철만
    • Journal of Environmental Science International
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    • v.12 no.7
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    • pp.725-733
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    • 2003
  • This survey was carried out to assess important species of phytoplankton in the ocean and bay in Korea from 32 references. The number of important species assessed from 50 genera 116 species. Of them, Bacillariophycea(diatoms) 34 genera 89 speceis(76.6%), Dinophyceae(dinoflagellates) 11 genera 22 species(18.9%), Chrysophyceae 2 genera 2 species(1.7%), Cyanophycea(blue-green algae), Raphidophyceae, Euglenophyceae(euglenoids) 1 genera 1 species(0.9%) respectively. By ecological characters, the number of dominant species were 79 species including Actinoptychus seranius, 50 species including Coscinodiscus centralis were recorded as frequently apperaing species and 36 species including Cochlodinium polykrikoides were recorded as red-tide causative organism. Also, 11 species including Prorocentrum micans were surveyed as the indicator including all ecological characters.

Bacterial Diversity in the Guts of Sea Cucumbers (Apostichopus japonicus) and Shrimps (Litopenaeus vannamei) Investigated with Tag-Encoded 454 Pyrosequencing of 16S rRNA Genes (16S rRNA 유전자의 454 파이로서열 분석을 이용한 해삼(Apostichopus japonicas)과 새우(Litopenaeus vannamei)의 장내 세균의 다양성 연구)

  • Noh, Eun Soo;Kim, Young-Sam;Kim, Dong-Hyun;Kim, Kyoung-Ho
    • Korean Journal of Microbiology
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    • v.49 no.3
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    • pp.237-244
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    • 2013
  • Bacterial diversities in the guts of sea cucumbers (Apostichopus japonicus) and shrimps (Litopenaeus vannamei) were investigated using barcoded or tag-encoded 454 pyrosequencing of 16S rRNA genes. In sea cucumbers, most of sequences were related to two genera, the genus Propionigenium in the phylum Fusobacteria and an unclassified genus in the family Flavobacteriaceae of phylum Bacteroidetes. Shrimps showed various kinds of genera including Lactococcus, Leuconostoc, Prochlorococcus, and Vibrio as well as the unclassified genera in the families, Flavobacteriaceae, Rhodobacteraceae, Desulfobulbaceae, and Helicobacteraceae and in the order Mycoplasmatales. Unclassified genera containing environmental sequences only are more than half of genera from sea cucumbers and shrimps. Sea cucumbers and shrimps could be unexplored sources of novel microbes and the bacterial diversity of them was revealed by high throughput 454 pyrosequencing.

A Herbological Study on the Plants of Crassulaceae in Korea (한국산 경천과(景天科) 식물에 관한 본초학적(本草學的) 연구)

  • Joo, Jung-Suk;Choi, Chan-Hun;Kim, Jeong-Sang;Kim, Chae-Hyun;Jeong, Jong-Gil
    • The Korea Journal of Herbology
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    • v.25 no.3
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    • pp.139-147
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    • 2010
  • Objectives:For the purpose of developing Korean herbalogy of the plants belonging to Crassulaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods:The examined herbalogical books and research papers which published at home and abroad. Results: 1. There were totaled to 6 genera and 39 species in Crassulaceae in Korea and among them medicinal plants are 5 genera, 14 species, some 36% in total. 2. Sedum genus is main kind enough that it has 20 species among 39 species in the Crassulaceae, of which medicinal plants are 9 species. 3. The herb is the main medicinal parts of medicinal plants in the Crassulaceae which is used in 14 species. 4. According to nature and flavor of medicinal plants in the Crassulaceae, they were classified into neutral 12 species; sour taste 14 in the order. 5. According to meridian propism of medicinal plants in the Crassulaceae, they were classified into liver meridian 13 species. 6. According to the properties and principal curative action in the Crassulaceae, they were classified into drugs for detoxicant 19 species, drugs for styptic 18, drugs for detumescence 14 in the order. 7. The number of toxic species in the Crassulaceae was examined to be 3 species. Conclusions:There were totaled to 6 genera and 39 species in Crassulaceae in Korea and among them medicinal plants are 5 genera, 14 species, some 36% in total.