• Title/Summary/Keyword: gas chromatography/mass spectrometry identification

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Identification of New 2-Deoxy Type Brassinosteroids in Immature Seed of Phaseolus vulgaris by Gas Chromatography-Mass Spectrometry

  • Kim, Seong-Ki
    • Journal of Plant Biology
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    • v.37 no.4
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    • pp.411-415
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    • 1994
  • In immature seeds of Phaseolus vulgaris, the presence of threee new 2-deoxy type brassinosteroids (trihydroxybrassinosteroids) have been demonstrated. These less polar brassinosteroids have been demonstrated. These less polar brassinosteroids have been tentatively characterized to be (3ξ, 22ξ, 23ξ)-2-deoxy-25-methylodolicholide, (3ξ, 22ξ, 23ξ)-2-deoxoy-dolichosterone and (3ξ, 22ξ, 23ξ)-2-deoxy-24-ethylbrassinone by analysis of gas chromatography-mass spectrometry. Their less biological activity and oxidation state than those of tetrahydroxybrassinosteroids suggest that they are potent biosynthetic precusors of tetrahydroxybrassinosteroids.

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Identification of urinary metabolite(s) of CKD-712 by gas chromatography/mass spectrometry in rats

  • Jean, Hee-Kyung;Choi, Hae-Yeon;Kim, Youn-Jung;Kwon, Oh-Seung;Ryu, Jae-Chun
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.314.1-314.1
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    • 2003
  • Examination was made of the urinary metabolite(s) of CKD-712, which is a chiral compound, named S-YS49 derived from higenamine (one component of Aconite spp.) derivatives. First of all. to analyze the metabolite(s) of CKD-712, a simple and sensitive detection method for CKD-712 was developed by using gas chromatography-mass spectrometry(GC/MS). (omitted)

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GC-MS and GC-FID Analysis of Citronella Oil Products for Indicator Ingredient Identification

  • Sumin Kang;Wooil Kim;Jin Wuk Lee;Sangwon Cha
    • Mass Spectrometry Letters
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    • v.14 no.4
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    • pp.160-165
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    • 2023
  • Citronella oil, an essential oil extracted through steam distillation from the leaves and stems of Cymbopogon, is a natural complex substance (NCS) regulated by the Korean government for its use in insect repellents. The component ratios of NCSs like citronella oil vary due to differences in manufacturing processes and origins, presenting a challenge in identifying and quantifying these substances in consumer chemical products. This study analysed ten commercially available products of the most commonly used types of citronella oil, specifically Java and Ceylon types, using gas chromatography (GC)-mass spectrometry (MS) and GC with flame ionization detection (FID). Through chromatographic data, we aimed to determine the components that can qualitatively identify citronella oil and the indicator ingredients that can be used for content analysis.

Identification of Propentofylline Metabolites in Rats by Gas Chromatography/Mass Spectrometry

  • Kwon, Oh-Seung;Ryu, Jae-Chun
    • Archives of Pharmacal Research
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    • v.23 no.4
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    • pp.374-380
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    • 2000
  • Propentofylline (PPF, 3-methyl-1-(5-oxohexyl)-7-propylxanthine) has been reported to be a compound for treatment of both vascular dementia and dementia of the Alzheimer type. The short half-life (about 15 min) of PPF at the terminal elimination phase and poor bioavailability after oral administration of PPF to rabbits (Kim et al., 1992) suggest in part that this drug takes the extensive first-pass metabolism in the liver. In addition, the metabolic pathway for PPF remains unclear. The objective of this experiment is to identify urinary metabolites of PPF in rats. For the identification of the metabolites, rat urine was collected after oral administration of 100${m}g/kg$ PPF. PPF metabolite, 3-methyl-1-(5-hydroxyhexyl)-7-propylxanthine, was synthesized and confirmed by gas chromatography/mass spectroscopy (GC/MS) and $^1H$ nuclear magnetic resonance spectroscopy. The urinary metabolites of PPF were extracted with diethyl ether and identified by electron impact and chemical ionization GC/MS. One urinary metabolite was confirmed to be 3-methyl-1-(5-hydroxyhexyl)-7-propylxanthine by synthesized authentic compound. Several metabolites of monohydroxy- and dihydroxy-PPF were identified based on mass fragmentation of both intact and trimethylsilylated derivatives of PPF metabolites and the novel structure of these metabolites is suggested based on mass spectra.

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Identification of Higenamine nad its Metabolites in Rat by Gas Chromatography/Mass Spectrometry

  • Ryu, Jae-chun;Song, Yun-Seon;Kim, Myung-Soo;Cho, Jung-Hyuck;Yunchoi, Hye-Sook
    • Archives of Pharmacal Research
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    • v.16 no.3
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    • pp.213-218
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    • 1993
  • ($\pm$)-Higenamine is known as a cardiotonic principle of aconite root (root of Aconitum spp., Ranunculaceae). A simple and sensitive detection method for higenamine was developed by using gas chromatography-mass spectrometry (GC/MS). The recovery of higenamine after extraction and concentration with XAD-2 resin column was around 95% from rat biological fluids such as bile, plasma and urine. The limits of detection of higenamine in these biological fluids were approximately 0.1 ng/ml each. It has well been suggested that tetrahydroisoquinolines possessing catechol moiety such as higenamine should be subjected to the catechol-O-methyl transferase (COMT) activity in vivo. We detected two major peaks of presumed metabolites of higenamine in the total ion chromatogram obtained from the rat urine sample after the oral adminstration of ($\pm$)-higenamine. The scan mass spectrum of one of the metabolties coincided with those obtained from coclaurine $(C_6$-O-methyl higenamine) and those of the other metabolite are suggestive of isococlaurine $(C_7$-O-methyl higenamine).

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Direct Analysis of Organic Additives in Cured Rubber by Pyrolysis-Gas Chromatography/Mass Spectrometry (열분해-가스크로마토그래피/질량분석법에 의한 가황고무중의 유기첨가제의 직접분석)

  • Kim, Seung Wook;Heo, Gwi Suk;Lee, Gae Ho
    • Journal of the Korean Chemical Society
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    • v.41 no.10
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    • pp.524-534
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    • 1997
  • Analysis of additives in cured rubber is often a difficult task for analytical chemists because of a wide variety of complex components. Conventional analyses of additives and rubbers have been done in multistep, off-line processes with large sample size and extensive sample preparations. The coumarone-indene resin, resorcinol-formaldehyde resin, and prevulcanization inhibitor have been characterized by their pyrolysis pathways and mass spectra of characteristic pyrolyzates. Pyrolysis Gas Chromatography/Mass Spectrometry (Py-GC/MS) was used in the identification of additives without any sample pretreatment. This result shows that several organic additives in cured rubber can be directly analyzed.

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On the Pyrolysis of Polymers II. Identification of the Products from Polymer Pyrolysis by Gas Chromatography (高分子物質의 熱分解에 關한 硏究 (第2報) Gas Chromatography 에 依한 熱分解生成物의 檢索)

  • Chwa-Kyung Sung
    • Journal of the Korean Chemical Society
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    • v.7 no.2
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    • pp.106-114
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    • 1963
  • The products from polymer pyrolysis at $450^{\circ}$ are cooled with ice, then liquid and gaseous portions are analysed by gas chromatography. Di-2-ethyl hexyl sebacate column, silicone oil column, silica gel column and tetraethyleneglycol dimethylether column, which was most effective for the separation of hydrocarbon gases, are used. Identification of isomers could be secured more effectively by gas chromatography than mass spectrometry. Elucidation of the mechanism for thermal decomposition of polymers could be done through the identification of pyrolysis products. Although more extensive work is needed, some patterns of polymer pyrolysis are discussed.

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Identification of Marker Compounds for Discriminating between Embryogenic and Nonembryogenic Calluses of Higher Plants Using Pyrolysis Gas Chromatography Mass Spectrometry and Genetic Programming

  • Kim Suk-Weon;Ban Sung-Hee;Yoo Ook-Joon;Liu Jang-Ryol
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.1
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    • pp.38-42
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    • 2006
  • When whole cells are subjected to pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) analysis, it provides biochemical profiles containing overlapping signals of the majority of compounds. To determine marker compounds that discriminate embryogenic calluses from nonembryogenic calluses, samples of embryogenic and nonembryogenic calluses of five higher plant species were subjected to Py-GC/MS. Genetic programming of Py-GC/MS data was able to discriminate embryogenic calluses from nonembryogenic calluses. The content ratio of 5-meyhyl-2-furancarboxaldehyde and 5-(hydroxymethyl)-2-furancarboxaldehyde was greater in nonembryogenic calluses than in embryogenic calluses. However, the content ratio of phenol, p-cresol, and $^1H-indole$ in embryogenic calluses was 1.2 to 2.4 times greater than the ratio in nonembryogenic calluses. These pyrolysates seem to be derived from the components of the cell walls, which suggests that differences in cell wall components or changes in the architecture of the cell wall playa crucial role in determining the embryogenic competence of calluses.