• Nuclear Engineering and Technology
• /
• 제47권3호
• /
• pp.380-387
• /
• 2015

A lead slowing-down spectrometer (LSDS) system is a promising nondestructive assay technique that enables a quantitative measurement of the isotopic contents of major fissile isotopes in spent nuclear fuel and its pyroprocessing counterparts, such as $^{235}U$, $^{239}Pu$, $^{241}Pu$, and, potentially, minor actinides. The LSDS system currently under development at the Korea Atomic Energy Research Institute (Daejeon, Korea) is planned to utilize a high-flux ($>10^{12}n/cm^2{\cdot}s$) neutron source comprised of a high-energy (30 MeV)/high-current (~2 A) electron beam and a heavy metal target, which results in a very intense and complex radiation field for the facility, thus demanding structural shielding to guarantee the safety. Optimization of the structural shielding design was conducted using MCNPX for neutron dose rate evaluation of several representative hypothetical designs. In order to satisfy the construction cost and neutron attenuation capability of the facility, while simultaneously achieving the aimed dose rate limit (< $0.06{\mu}Sv/h$), a few shielding materials [high-density polyethylene (HDPE)eBorax, $B_4C$, and $Li_2CO_3$] were considered for the main neutron absorber layer, which is encapsulated within the double-sided concrete wall. The MCNP simulation indicated that HDPE-Borax is the most efficient among the aforementioned candidate materials, and the combined thickness of the shielding layers should exceed 100 cm to satisfy the dose limit on the outside surface of the shielding wall of the facility when limiting the thickness of the HDPE-Borax intermediate layer to below 5 cm. However, the shielding wall must include the instrumentation and installation holes for the LSDS system. The radiation leakage through the holes was substantially mitigated by adopting a zigzag-shape with concrete covers on both sides. The suggested optimized design of the shielding structure satisfies the dose rate limit and can be used for the construction of a facility in the near future.

• Journal of Forest and Environmental Science
• /
• 제29권3호
• /
• pp.200-210
• /
• 2013

Biochemical characteristics of 24 Pongamia pinnata genotypes (candidate plus trees) from three agroclimatic zones were estimated and molecular characterization through RAPD markers was done. Various biochemical characters viz. seed oil, total carbohydrates, protein, acid value and Iodine number recorded significant variation among different genotypes. The highest seed oil content was 41.87% while seeds of 14 genotypes recorded above average (32.11%) for the trait. Seed oil and protein content exhibited a significant positive correlation and moderate heritability. Out of the initially selected twenty-five random primers, twenty-two RAPD primers were found to be highly reproducible and produced a total of 183 loci of which 147 (80.32%) loci were polymorphic. Percentage of polymorphism varied from 44% to 100% with an average of 80.62%. High level of genetic variation was found among different genotypes of P. pinnata. Both molecular and oil content (biochemical) markers appeared useful in analyzing the extent of genetic diversity in Pongamia and the result of these analyses will help to better understand the genetic diversity and relationship among populations. Overall, the Pongamia genotypes included in the study showed a correlation with their geographical origins such that genotypes from the same region tend to have higher genetic similarity as compared to those from different regions. However, in UPGMA based Nei's analysis, some genotypes were found not to be grouped based on geographical origins possibly due to the exchange of germplasm over time between farmers across the regions. The results from oil content analyses showed that several genotypes in 'Central and Western Plateau' agroclimatic zone of Jharkhand displayed a good potential for high oil content. The study provides insight about P. pinnata populations in Jharkhand (India) and constitutes a set of useful background information that can be used as a basis for future breeding strategy and improvement of the species.

• 한국인터넷방송통신학회논문지
• /
• 제21권3호
• /
• pp.99-105
• /
• 2021

넥플릭스,유튜브로 대표되는 OTT 동영상 제작 서비스에 인공지능으로 콘텐츠를 개인별 맞춤식 추천 시스템은 보편화 되었다. 유튜브의 개인별 맞춤 추천서비스 시스템은 두 개의 신경망으로 구성되는데 신경망 하나는 추천 후보생성 모델이고 다른 하나는 순위평가 네트워크로 구성된다. Netflix의 동영상 추천 시스템은 두 개 데이터 분류 시스템으로 구성되어 있으며 콘텐츠 기반 필터링과 협업 필터링으로 나누어진다. 코로나 펜데믹으로 온라인 플랫폼 주도의 콘텐츠 제작이 활성화 되면서 인공지능을 활용한 가상 인플루언서 분야가 부각되고 있다. 가상인플루언서는 GAN(Generative Adversarial Networks) 인공지능으로 제작되는데 성격이 다른 두 시스템이 서로 경쟁하는 방식으로 학습이 반복되는 비교사(Unsupervised) 학습 알고리즘이다. 이 연구는 AI 개인별 추천 기반 플랫폼과 가상인플루언서(메타버스)가 향후 OTT의 핵심콘텐츠로의 발전 가능성도 연구해 보았다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.261-261
• /
• 2022

Cowpea [Vigna unguiculata (L.) Walp] is one of the most important grain legumes that enhance soil fertility and is well-adapted to various abiotic stress. Also, it is cultivated worldwide as a tropical annual crop, and the semi-arid regions are known as the main cowpea-produced regions. However, accumulation of soil salinity induced by low rainfall in these regions is reducing crop yields and quality. In general, plants exposed to soil salinity cause an accumulation of high ion chloride, which leads to the degradation of root and leaf proteins. In this study, we identified candidate genes associated with salinity tolerance through an analysis of differentially expressed genes (DEGs) in four cowpea germplasms with contrasting salinity tolerance. A total of 553,776,035 short reads were obtained using the Illumina Novaseq 6000 platform for RNA-Seq, which were subsequently aligned to the reference genome of cowpea Vunguiculata v1.2. A total of9,806 DEGs were identified between NaCl treatment and control of four cowpea germplasms. Among these DEGs, functions related to salt stress such as calcium transporter and cytochrome-450 family were associated with salt stress. In GO analysis and KEGG analysis, these DEGs were enriched in terms such as the "phosphorylation", ''extracellular region", and "ion binding". These RNA-seq results will improve the understanding of the salt tolerance of cowpea and can be used as useful basic data for molecular breeding technology in the future.

• 한국동물생명공학회지
• /
• 제38권3호
• /
• pp.158-166
• /
• 2023

Background: Copy number variation (CNV) can be identified using next-generation sequencing and microarray technologies, the research on the analysis of its association with meat traits in livestock breeding has significantly increased in recent years. Hanwoo is an inherent species raised in the Republic of Korea. It is now considered one of the most economically important species and a major food source mainly used for meat (Hanwoo beef). Methods: In this study, CNVs and the relationship between the obtained CNV regions (CNVRs) can be identified in the Hanwoo steer samples (n = 473) using Illumina Hanwoo SNP 50K bead chip and bioinformatic tools, which were used to locate the required data and meat traits were investigated. The PennCNV software was used for the identification of CNVs, followed by the use of the CNV Ruler software for locating the different CNVRs. Furthermore, bioinformatics analysis was performed. Results: We found a total of 2,575 autosomal CNVs (933 losses, 1,642 gains) and 416 CNVRs (289 gains, 111 losses, and 16 mixed), which were established with ranged in size from 2,183 bp to 983,333 bp and 10,004 bp to 381,836 bp, respectively. Upon analyzing the restriction of minor alleles frequency > 0.05 for meat traits association, 6 CNVRs in the carcass weight, 2 CNVRs in the marbling score, 3 CNVRs in the backfat thickness, and 2 CNVRs in the longissimus muscle area were related to the meat traits. In addition, we identified an overlap of 347 CNVRs. Moreover, 3 CNVRs were determined to have a gene that affects meat quality. Conclusions: Our results confirmed the relationship between Hanwoo CNVR and meat traits, and the possibility of overlapping candidate genes, annotations, and quantitative trait loci that results depended on to contribute to the greater understanding of CNVs in Hanwoo and its role in genetic variation among cattle livestock.

• Biomolecules & Therapeutics
• /
• 제32권4호
• /
• pp.467-473
• /
• 2024

In this study, we investigated the potential protective effects of (+)-afzelechin (AZC), a natural compound that is derived from Bergenia ligulata, on lipopolysaccharide (LPS)-induced inflammatory responses. AZC is known to have antioxidant, anticancer, antimicrobial, and cardiovascular protective properties. However, knowledge regarding the therapeutic potential of AZC against LPS-induced inflammatory responses is limited. Thus, we investigated the protective attributes of AZC against inflammatory damage caused by LPS exposure. We examined the effects of AZC on heme oxygenase (HO)-1, cyclooxygenase (COX)-2, and inducible nitric oxide synthase (iNOS) in LPS-activated human umbilical vein endothelial cells (HUVECs). In addition, the effects of AZC on the expression of iNOS, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were analyzed in the lung tissues of LPS-injected mice. Data revealed that AZC promoted the production of HO-1, inhibited the interaction between luciferase and nuclear factor (NF)-κB, and reduced the levels of COX-2/PGE2 and iNOS/NO, thereby leading to a decrease in the signal transducer and activator of transcription (STAT)-1 phosphorylation. Moreover, AZC facilitated the nuclear translocation of Nrf2, increased the binding activity between Nrf2 and the antioxidant response elements (AREs), and lowered the expression of IL-1β in the LPS-treated HUVECs. In the animal model, AZC significantly reduced the expression of iNOS in the lung tissue structure and the TNF-α level in the bronchoalveolar lavage fluid. These findings demonstrate that AZC possesses anti-inflammatory properties that regulate iNOS through the inhibition of both NF-κB expression and p-STAT-1. Consequently, AZC has potential as a future candidate for the development of new clinical substances for the treatment of pathological inflammation.

• Molecules and Cells
• /
• 제38권8호
• /
• pp.685-696
• /
• 2015

Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.

• 한국전자거래학회지
• /
• 제23권4호
• /
• pp.109-126
• /
• 2018

축산업 분야는 대량적이고 밀집적인 생산이 가능하기 때문에 양돈 양계 오리를 중심으로 대규모 형태의 자본 집약적인 산업으로 빠르게 진행되고 있다. 하지만 전염병이 급격히 확산되면 축산업과 국민 생활에 심각한 위협이 될 수 있다. 이에 대비하기 위해 국가는 가축 방역 5단계에 맞추어 2013년 국가동물방역통합시스템(KAHIS)을 구축하여 운영하고 있다. 이에 따라 구축된 디지털화된 데이터와 정보는 관리의 용이성이 뛰어나지만, 유기적 연계를 통한 대책 마련이 쉽지 않다는 어려움이 지적되고 있다. 최근 사물인터넷(IoT), 빅데이터, 인공지능(AI) 등과 같은 제4차 산업혁명 시대의 기술이 빠르게 발전하면서 이를 도입하여 스마트 가축방역으로의 발전이 추진되고 있다. 이에 따라 본 연구는 5개 방역단계별로 국내외 4차 산업혁명 기술 적용 현황을 조사하여 가까운 시일 내에 실천 가능한 후보 과제 13개를 도출하였다. 정책 수립의 우선순위를 확인하기 위하여 전문가 집단을 대상으로 조사하여 5개 방역단계의 우선순위와 각 단계별 우선순위를 조사하였다. 본 연구의 결과물은 스마트 가축방역 연구, 가축방역 분야의 선진화를 위한 정책 수립에 도움이 될 것으로 기대한다.

• 대한원격탐사학회지
• /
• 제37권5_2호
• /
• pp.1245-1257
• /
• 2021

세계 최초의 정지궤도 해색관측 위성인 GOCI의 임무를 승계한 천리안위성 2B호의 해양탑재체인 GOCI-II가 2020년 2월 발사되어 같은 해 10월부터 정규 운영되고 있다. 한국해양과학기술원은 실시간 수신한 GOCI-II 원시자료를 Level 1B와 26종 Level 2 산출물로 처리하며, 이 자료들은 국립해양조사원을 통해 서비스된다. 이 논문에서는 정규 운영 1년차의 위성자료 운영 현황을 소개하고, 향후 개선 방향을 제시하고자 하였다. GOCI-II의 기본 해색 산출물인 엽록소 농도, 총 부유물질 농도, 용존유기물 농도 산출물은 OC4, YOC 알고리즘으로 처리 중이며, 그 수식 및 프로세스에 대해 상세 기술하였다. GOCI-II에서 새롭게 추가된 전구 관측은 궤도상 시험운영기간 동안 태양천정각과 sun glint만 고려하여 관측 스케줄이 수립되었으나, 양질의 Level 2 산출물 생산을 위해 조건을 세분화하고 위성 천정각을 추가 고려하여 개선하였다. 그 결과 'Best Ocean'을 만족하는 슬롯의 개수가 15에서 78개로 대폭 증가하고, 'Bad Ocean'에 해당하는 슬롯이 55개에서 13개로 크게 감소하였다. GOCI-II의 산출물의 품질관리를 위해서 유럽우주국에서 정의하는 요구사항을 기반으로 GOCI-II 검보정 요구사항을 제시하였다. 그리고 GOCI 검보정 사이트를 기반으로 하되, 향상된 위성 스펙을 고려하여 지역 관측 검보정을 위한 추가 고정점 검보정 사이트 후보지를 제시하였다. 전구관측 자료의 품질관리는 국내외 해양인프라를 구축하고 있는 한국해양과학기술원의 연구선과 해외 기지를 활용하되, 국외 해역의 현장관측 자료 획득을 위해서는 GOCI-II 국제 검보정 네트워크 구축이 필요할 것으로 판단된다. 이러한 결과는 위성자료 사용자들의 산출물 처리에 대한 이해를 높이고, 향후 위성자료 품질관리 업무 수행 상세계획 수립에 도움이 될 것으로 기대된다.

• Journal of Animal Science and Technology
• /
• 제52권6호
• /
• pp.475-480
• /
• 2010

The bovine fatty acid binding protein 4 (FABP4) plays an important role to uptake intracellular fatty acid. It has been previously reported as a positional candidate gene for marbling score in livestock. The re-sequencing of FABP4 gene detected a polymorphic AT repeated sequence in intron II of FABP4 gene. Allelic distribution for this microsatellite marker was examined in other cattle breeds. A total of 8 alleles were detected with diverse repeat units (14 to 21 AT repeat) in Hanwoo and 7 breeds. Of the 8 alleles, the predominant alleles were $[AT]_{16}$, $[AT]_{18}$ and $[AT]_{19}$ in the Hanwoo and 7 cattle breeds. The linear mixed model for genotypic effect (3237AT) on carcass traits showed a significant effect on marbling score (MAR P=0.025) and live weight (LWT; P=0.04) in the 583 Hanwoo cattle population. Live weight (LW) was highest in the homozygous $(AT)_{17}$ genotype ($557.5{\pm}6.94$) and lowest in the heterozygous $(AT)_{16/17}$ genotype ($521.7{\pm}7.70$). On the other hand, the homozygous $(AT)_{17}$ genotype ($3.0{\pm}0.15$) has the highest effect on marbling score and the lowest effect was in homozygous (AT)$_{18}$ genotype ($2.2{\pm}0.15$). The marbling score difference between both groups was 0.8 which is around two times higher than SNP genotype effect on marbling score in Limousin $\times$ Wagyu crosses.