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http://dx.doi.org/10.14348/molcells.2015.0033

Proteomic Analysis to Identify Tightly-Bound Cell Wall Protein in Rice Calli  

Cho, Won Kyong (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Hyun, Tae Kyung (Department of Industrial Plant Science and Technology,College of Agricultural, Life and Environmental Sciences, Chungbuk National University)
Kumar, Dhinesh (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Rim, Yeonggil (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Chen, Xiong Yan (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Jo, Yeonhwa (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Kim, Suwha (Department of Life Science, Gwangju Institute of Science and Technology)
Lee, Keun Woo (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Park, Zee-Yong (Department of Life Science, Gwangju Institute of Science and Technology)
Lucas, William J. (Department of Plant Biology, University of California)
Kim, Jae-Yean (Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Publication Information
Molecules and Cells / v.38, no.8, 2015 , pp. 685-696 More about this Journal
Abstract
Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.
Keywords
callus; cell wall proteins; MudPIT; proteomics; rice;
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