• Title/Summary/Keyword: fusion center

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Ubiquitin Fusion System for Recombinant Peptide Expression and Purification: Application to the Cytoplasmic Domain of Syndecan-4

  • Chae, Young-Kee;Lee, Ha-Yan;Lee, Weon-Tae
    • Bulletin of the Korean Chemical Society
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    • v.28 no.9
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    • pp.1549-1552
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    • 2007
  • The cytoplasmic domain of syndecan-4, a type I transmembrane heparan sulfate proteoglycan, was overexpressed as a fused form with the ubiquitin molecule in Escherichia coli, and the fusion protein was purified using immobilized metal affinity chromatography (IMAC). The cytoplasmic domain was released from its fusion partner by using yeast ubiquitin hydrolase (YUH), and subsequently purified by reverse phase chromatography. The integrity of the resulting peptide fragment was checked by MALDI-TOF and NMR spectroscopy. The yield of the peptide was 3.0-1.5 mg per liter in LB or minimal medium, respectively. The recombinant expression and purification of this domain will enable us its structural and functional studies using multidimensional NMR spectroscopy.

The Efficacy of Titanium Mesh Cage in Tuberculous Spondylitis Treated with Anterior Intervertebral Fusion (척추 결핵의 전방유합술시 Titanium Mesh Cage의 효과)

  • Jeong, Ju-Ho;Lee, Sang-Gu;Yoo, Chan-Jong;Han, Ki-Soo;Kim, Woo-Kyung;Kim, Young-Bo;Park, Cheol-Wan;Lee, Uhn
    • Journal of Korean Neurosurgical Society
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    • v.30 no.8
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    • pp.998-1003
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    • 2001
  • Objective : The maintenance of the correction of kyphotic deformity is one of the difficult problem in tuberculous spondylitis after anterior debriment and fusion with tricortical bone graft. The goal of this study is to find out the efficacy of titanium mesh cage impacted with autogenous bone chip in tuberculous spondylitis treated with anterior intervertebral fusion. Materials and Method : Twelve patients were treated with anterior intervertebral fusion using titanium mesh cage for tuberculous spondylitis from January 1996 to June 1999. We analized the changes in the correction of kyphotic deformity, changes of ESR and CRP, fusion state and recurrence after anterior intervertebral fusion with titanium mesh cage. Results : Clinical symptoms were improved in all twelve patients without any neurologic complications. The mean kyphotic angle corrected was 7.3 degrees immediately after operation, but the loss of correction of kyphotic angle was 2.2 degrees after 3 months and 2.6 degrees after 6 months. We found that the loss of correction of kyphotic deformity occurred mainly within the first 3 months after surgery. Only one patient, suffered from acute hepatic failure after first operation and had an insufficient anti-tuberculous medication therapy, showed recurrence of tuberculous spondylitis after 6 months. The patient underwent a second operation with posterior fixation procedure with good outcome. The changes of ESR and CRP were not specifically important factor to reveal recurrence of tuberculosis of the spine in our series. Conclusion : The surgical procedure of tuberculous spondylitis using titanium mesh cage with bone chip seems to be an effective procedure to minimize loss of the correction of kyphotic deformity without any aggravating inflammatory change and recurrence with titanium mesh cage, when sufficient debridement and anti-tuberculous chemotherapy are achieved.

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Fusion algorithm for Integrated Face and Gait Identification (얼굴과 발걸음을 결합한 인식)

  • Nizami, Imran Fareed;Hong, Sug-Jun;Lee, Hee-Sung;Ann, Toh-Kar;Kim, Eun-Tai;Park, Mig-Non
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2007.11a
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    • pp.15-18
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    • 2007
  • Identification of humans from multiple view points is an important task for surveillance and security purposes. For optimal performance the system should use the maximum information available from sensors. Multimodal biometric systems are capable of utilizing more than one physiological or behavioral characteristic for enrollment, verification, or identification. Since gait alone is not yet established as a very distinctive feature, this paper presents an approach to fuse face and gait for identification. In this paper we will use the single camera case i.e. both the face and gait recognition is done using the same set of images captured by a single camera. The aim of this paper is to improve the performance of the system by utilizing the maximum amount of information available in the images. Fusion is considered at decision level. The proposed algorithm is tested on the NLPR database.

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Reducing Spectral Signature Confusion of Optical Sensor-based Land Cover Using SAR-Optical Image Fusion Techniques

  • ;Tateishi, Ryutaro;Wikantika, Ketut;M.A., Mohammed Aslam
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.107-109
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    • 2003
  • Optical sensor-based land cover categories produce spectral signature confusion along with degraded classification accuracy. In the classification tasks, the goal of fusing data from different sensors is to reduce the classification error rate obtained by single source classification. This paper describes the result of land cover/land use classification derived from solely of Landsat TM (TM) and multisensor image fusion between JERS 1 SAR (JERS) and TM data. The best radar data manipulation is fused with TM through various techniques. Classification results are relatively good. The highest Kappa Coefficient is derived from classification using principal component analysis-high pass filtering (PCA+HPF) technique with the Overall Accuracy significantly high.

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Fuctionalization of SBA-16 Mesoporous Materials with Cobalt(III) Cage Amine Complex

  • Han, Sang-Cheol;Sujandi, Sujandi;Park, Sang-Eon
    • Bulletin of the Korean Chemical Society
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    • v.26 no.9
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    • pp.1381-1384
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    • 2005
  • Surface modification of tridimensional cubic mesoporous silica, SBA-16, was investigated with pendant arm functionalized cobalt diaminosarcophagine (diAMsar) cage complex which covalently grafted onto the silica surface through the silication with sylanol group. The spectroscopic results showed that the mesoporous structure was preserved under the $[Co(diAMsar)]^{3+}$ grafting reaction condition. Successful grafting prevented the cobalt diAMsar cage from leaching out from the SBA-16 support.

Future trends in multisensor integration and fusion

  • Luo, Ren-C.;Kay, Michael-G.;Lee, W.Gary
    • 제어로봇시스템학회:학술대회논문집
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    • 1992.10b
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    • pp.22-28
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    • 1992
  • The need for intelligent systems that can operate in an unstructured, dynamic environment has created a growing demand for the use of multiple, distributed sensors. While most research in multisensor fusion has revolved around applications in object recognition-including military applications for automatic target recognition-developments in microsensor technology are encouraging more research in affordable, highly-redundant sensor networks. Three trends that are described at length are the increasing use of microsensors, the techniques that are used in the handling of partial or uncertain data, and the application of neural network techniques for sensor fusion.

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CoMFA Based Quantitative Structure Toxicity Relationship of Azo Dyes

  • Pasha, F.A.;Nam, Kee-Dal;Cho, Seung-Joo
    • Molecular & Cellular Toxicology
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    • v.3 no.2
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    • pp.145-149
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    • 2007
  • Studies of relationship between structure and toxicity of azo dyes have been performed with comparative molecular field analysis (CoMFA) techniques. 3D QSTR analyses indicate that the steric and electrostatic interactions are important. The steric field based model gives strong correlation ($q^2$=0.57, $r^2$= 0.92). The steric field in conjunction with electrostatic field give more strong correlation ($q^2$=0.57, $r^2$=0.95). All study indicates that a bulky and electronegative group at benzene ring and a small group at position 3 of aniline ring might be significant to reduce the mutagenicity.

A Method of Preparing Recombinant Fusion Antigen from Rotavirus and Norovirus

  • Oh, Ho-Kyung;Huh, Chul-Sung;Baek, Young-Jin;Yoo, Dong-Wan
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2004.05a
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    • pp.353-356
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    • 2004
  • Rotavirus and Norovirus are major causative agents of acute diarrhea and gastroenteritis. In our study, Each viral RNA was isolated from the feces of patients for viral diarrhea in Korea, respectively. And cDNA library were constructed using RT-PCR. Also, cDNAs encoding VP8 derived from Rotavirus and Capsid protein derived from norovirus were subesequently cloned and expressed in Echerichia coli as a fusion antigen. Molecular weight of fusion antigen was approximately 60kDa. Also, substantial overexpression was accomplished. We yielded egg yolk lgY which is potentially useful in controlling of Rotavirus and Norovirus which are one of the most prevalent pathogenic viruses.

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