• Title/Summary/Keyword: fusion

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Bifunctional Recombinant Fusion Enzyme Between Maltooligosyltrehalose Synthase and Maltooligosyltrehalose Trehalohydrolase of Thermophilic Microorganism Metallosphaera hakonensis

  • Seo, Ju-Seok;An, Ju-Hee;Cheong, Jong-Joo;Choi, Yang-Do;Kim, Chung-Ho
    • Journal of Microbiology and Biotechnology
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    • v.18 no.9
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    • pp.1544-1549
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    • 2008
  • MhMTS and MhMTH are trehalose ($\alpha$-D-glucopyranosyl-[1,1]-$\alpha$-D-glucopyranose) biosynthesis genes of the thermophilic microorganism Metallosphaera hakonensis, and encode a maltooligosyltrehalose synthase (MhMTS) and a maltooligosyltrehalose trehalohydrolase (MhMTH), respectively. In this study, the two genes were fused in-frame in a recombinant DNA, and expressed in Escherichia coli to produce a bifunctional fusion enzyme, MhMTSH. Similar to the two-step reactions with MhMTS and MhMTH, the fusion enzyme catalyzed the sequential reactions on maltopentaose, maltotriosyltrehalose formation, and following hydrolysis, producing trehalose and maltotriose. Optimum conditions for the fusion enzyme-catalyzed trehalose synthesis were around $70^{\circ}C$ and pH 5.0-6.0. The MhMTSH fusion enzyme exhibited a high degree of thermostability, retaining 80% of the activity when pre-incubated at $70^{\circ}C$ for 48 h. The stability was gradually abolished by incubating the fusion enzyme at above $80^{\circ}C$. The MhMTSH fusion enzyme was active on various sizes of maltooligosaccharides, extending its substrate specificity to soluble starch, the most abundant natural source of trehalose production.

A Study on the Importance, Satisfaction, Perception and Intake Frequency of Fusion Menu (퓨전 메뉴의 중요도, 만족도, 인지도 및 섭취 빈도에 관한 연구)

  • Kang, Hye-Jung;Lee, Yeon-Jung
    • Culinary science and hospitality research
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    • v.14 no.4
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    • pp.134-149
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    • 2008
  • This thesis is aimed to analyze the importance, satisfaction, perception and intake frequency of fusion menu in order to develop the market segmentations and marketing strategies for useful information on the fusion menu and its improvement in the food industries. The findings of the study are as follows: First, the study revealed that diet menu(low fat, low-cal) and vegetarian menu items have more influence on females than males in regard to the importance of fusion menu when examining gender. Second, the study revealed that Bulgogi pizza, Bulgogi burger, cheese cutlet, cheese kimbab, sweet and sour pork items have great influence on customers in their 10s while green tea latte, rice burger, Bulgogi pizza, kimchi hamburger items have a high effect on customers in their 20s in regard to the perception of fusion menu when examining age. Finally, the study revealed that the taste of food, the cleanliness of vessels, food hygiene, the freshness of food, the quality of menu, the portions of food, the nutrition of food, the speed of food service, food material harmony, the temperature of food, the flavor of food, distinctions from existing food, environment-friendly organic agriculture food material items have maintaining the good performance of fusion menus. It also showed that various strategies for price of fusion menu should be made when examining the IPA analysis.

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Fusion of Decisions in Wireless Sensor Networks under Non-Gaussian Noise Channels at Large SNR (비 정규 분포 잡음 채널에서 높은 신호 대 잡음비를 갖는 무선 센서 네트워크의 정보 융합)

  • Park, Jin-Tae;Kim, Gi-Sung;Kim, Ki-Seon
    • Journal of the Korea Institute of Military Science and Technology
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    • v.12 no.5
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    • pp.577-584
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    • 2009
  • Fusion of decisions in wireless sensor networks having flexibility on energy efficiency is studied in this paper. Two representative distributions, the generalized Gaussian and $\alpha$-stable probability density functions, are used to model non-Gaussian noise channels. By incorporating noise channels into the parallel fusion model, the optimal fusion rules are represented and suboptimal fusion rules are derived by using a large signal-to-noise ratio(SNR) approximation. For both distributions, the obtained suboptimal fusion rules are same and have equivalent form to the Chair-Varshney fusion rule(CVR). Thus, the CVR does not depend on the behavior of noise distributions that belong to the generalized Gaussian and $\alpha$-stable probability density functions. The simulation results show the suboptimality of the CVR at large SNRs.

A Study on Modern Fusion Up-style with a Motive Japanese Up-style (일본 업스타일을 모티브로한 현대 퓨전 업스타일의 연구)

  • Han, Sung-Jin;Kim, Sung-Nam
    • Journal of the Korean Society of Fashion and Beauty
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    • v.5 no.2 s.13
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    • pp.12-22
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    • 2007
  • Even though Up style field generally rise economic development and GNP, many demand got to cultural development and direct ratio. When you up style is main study of concrete study purpose which end of hair. Variation is upper spread, down spread, middle spread, symetric form, asymetric from etc. As a result is followed; 1. According to chignon's position, fusion style reproduced korea modem through various data and categorize style. 2. Modem korea fusion style reproduced theme up style(wedding, evening, causal, han-bok, fusion show). 3. The thesis compared and analyzed modem korea fusion up style and japanese fusion up style. (1) According to chignon's position and balance, up style compared and analyzed it. (2) Theme up style(wedding, evening, causal, han-bok, fusion show) compared and analyzed categorize. End of hair classified variation according to position and balance external image affected to it which bale on design component as a result, classified up-style reconstruct.

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A Novel Multifocus Image Fusion Algorithm Based on Nonsubsampled Contourlet Transform

  • Liu, Cuiyin;Cheng, Peng;Chen, Shu-Qing;Wang, Cuiwei;Xiang, Fenghong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.3
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    • pp.539-557
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    • 2013
  • A novel multifocus image fusion algorithm based on NSCT is proposed in this paper. In order to not only attain the image focusing properties and more visual information in the fused image, but also sensitive to the human visual perception, a local multidirection variance (LEOV) fusion rule is proposed for lowpass subband coefficient. In order to introduce more visual saliency, a modified local contrast is defined. In addition, according to the feature of distribution of highpass subband coefficients, a direction vector is proposed to constrain the modified local contrast and construct the new fusion rule for highpass subband coefficients selection The NSCT is a flexible multiscale, multidirection, and shift-invariant tool for image decomposition, which can be implemented via the atrous algorithm. The proposed fusion algorithm based on NSCT not only can prevent artifacts and erroneous from introducing into the fused image, but also can eliminate 'block effect' and 'frequency aliasing' phenomenon. Experimental results show that the proposed method achieved better fusion results than wavelet-based and CT-based fusion method in contrast and clarity.

Analysis of fusogenic activity of autographa californica nuclear polyhedrosis virus (Ac NPV) gp64 envelope glycoprotein

  • Kim, Hee-Jin;Yang, Jai-Myung
    • Journal of Microbiology
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    • v.34 no.1
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    • pp.7-14
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    • 1996
  • Teh baculovirus gp64 glycoprotein is a major component of the envelope of budded virus (BV) and has been shown that it plays an essential role in the infection process, especially virus-cell membrane fusion. We have cloned Autographa californica Nuclear Polyhedrosis Virus (AcNPV) gp64 protein were examined for membrane fusion activity by using a synchtium formation assay under various conditions. The optimal conditions required for inducing membrane fusion are 1) form pH 4.0 to 4.8 2) 15 min exposure of cells to acidic pH 3) at least 1 .mu.g of gp64 cloned plasmid DNA per 3 * 10$^{6}$ cells 4) and an exposure of cells to acidic pH at 72 h post-transfection. In order to investigate the role of hydrophobicity of the gp64 glycoprotein for the membrane fusion, the two leucine residues (amino acid position at 229 and 230) within hydrophobic region I were substituted to alanine by PCR-derived site-directed mutagenisis and the membrane fusion activity of the mutant was anlaysed. The gp64 glycoprotein carrying double alamine substitution mutation showed no significant difference in fusion activity. This result suggested that minor changes in hydrophobicity at the amino acid position 229 and 230 does not affect the acid-induced membrane fusion activity of the gp64 glycoprotein.

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The Sensory-Motor Fusion System for Object Tracking (이동 물체를 추적하기 위한 감각 운동 융합 시스템 설계)

  • Lee, Sang-Hee;Wee, Jae-Woo;Lee, Chong-Ho
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.52 no.3
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    • pp.181-187
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    • 2003
  • For the moving objects with environmental sensors such as object tracking moving robot with audio and video sensors, environmental information acquired from sensors keep changing according to movements of objects. In such case, due to lack of adaptability and system complexity, conventional control schemes show limitations on control performance, and therefore, sensory-motor systems, which can intuitively respond to various types of environmental information, are desirable. And also, to improve the system robustness, it is desirable to fuse more than two types of sensory information simultaneously. In this paper, based on Braitenberg's model, we propose a sensory-motor based fusion system, which can trace the moving objects adaptively to environmental changes. With the nature of direct connecting structure, sensory-motor based fusion system can control each motor simultaneously, and the neural networks are used to fuse information from various types of sensors. And also, even if the system receives noisy information from one sensor, the system still robustly works with information from other sensors which compensates the noisy information through sensor fusion. In order to examine the performance, sensory-motor based fusion model is applied to object-tracking four-foot robot equipped with audio and video sensors. The experimental results show that the sensory-motor based fusion system can tract moving objects robustly with simpler control mechanism than model-based control approaches.

Multi-Frame Face Classification with Decision-Level Fusion based on Photon-Counting Linear Discriminant Analysis

  • Yeom, Seokwon
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.14 no.4
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    • pp.332-339
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    • 2014
  • Face classification has wide applications in security and surveillance. However, this technique presents various challenges caused by pose, illumination, and expression changes. Face recognition with long-distance images involves additional challenges, owing to focusing problems and motion blurring. Multiple frames under varying spatial or temporal settings can acquire additional information, which can be used to achieve improved classification performance. This study investigates the effectiveness of multi-frame decision-level fusion with photon-counting linear discriminant analysis. Multiple frames generate multiple scores for each class. The fusion process comprises three stages: score normalization, score validation, and score combination. Candidate scores are selected during the score validation process, after the scores are normalized. The score validation process removes bad scores that can degrade the final output. The selected candidate scores are combined using one of the following fusion rules: maximum, averaging, and majority voting. Degraded facial images are employed to demonstrate the robustness of multi-frame decision-level fusion in harsh environments. Out-of-focus and motion blurring point-spread functions are applied to the test images, to simulate long-distance acquisition. Experimental results with three facial data sets indicate the efficiency of the proposed decision-level fusion scheme.

Abnormal Behavior in Color Tracking in the Fringe-Field Switching (FFS) Liquid Crystal Display

  • Jung, Jun-Ho;Ha, Kyung-Su;Chae, Mi-Na;Cho, In-Young;Kim, Woo-Il;Kim, Dae-Hyun;Kim, Sung-Min;Lee, Seung-Hee
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.616-619
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    • 2009
  • Color tracking behavior of in the fringe-field switching (FFS) mode using a liquid crystal with positive dielectric anisotropy has been studied. In the in-plane switching and vertical alignment devices, color chromaticity at normal direction changes from bluish to yellowish white linearly with increasing grey levels from dark to white state. Interestingly, abnormal behavior in color tracking is observed in FFS devices using a liquid crystal with positive dielectric anisotropy, that is, it changes from bluish to yellowish up to a certain middle grey level but turns over to bluish white with further increasing from a grey level to a fully white state. In this paper, we analyze this abnormal effect from the calculated and experimental results.

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Generalized IHS-Based Satellite Imagery Fusion Using Spectral Response Functions

  • Kim, Yong-Hyun;Eo, Yang-Dam;Kim, Youn-Soo;Kim, Yong-Il
    • ETRI Journal
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    • v.33 no.4
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    • pp.497-505
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    • 2011
  • Image fusion is a technical method to integrate the spatial details of the high-resolution panchromatic (HRP) image and the spectral information of low-resolution multispectral (LRM) images to produce high-resolution multispectral images. The most important point in image fusion is enhancing the spatial details of the HRP image and simultaneously maintaining the spectral information of the LRM images. This implies that the physical characteristics of a satellite sensor should be considered in the fusion process. Also, to fuse massive satellite images, the fusion method should have low computation costs. In this paper, we propose a fast and efficient satellite image fusion method. The proposed method uses the spectral response functions of a satellite sensor; thus, it rationally reflects the physical characteristics of the satellite sensor to the fused image. As a result, the proposed method provides high-quality fused images in terms of spectral and spatial evaluations. The experimental results of IKONOS images indicate that the proposed method outperforms the intensity-hue-saturation and wavelet-based methods.