• ALGAE
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• 제32권1호
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• pp.75-86
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• 2017

Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron spin resonance spectrometry and for intracellular reactive oxygen species scavenging activity in the Chang liver cell line. Characterization of CPs was done by Fourier transform infrared (FTIR) spectroscopy and by analysis of the monosaccharide composition. Time-dependent density functional theory quantum-chemical calculations at the RB3LYP/6-31G(d,p) level for constructed dimeric units of the corresponding polysaccharides were used to resolve the FTIR spectra. CPs from Chnoospora minima showed the highest DPPH and alkyl radical scavenging activities and higher intracellular reactive oxygen species scavenging effects for both AAPH and $H_2O_2$ induced ROS production in "Chang" cells. The major polysaccharide constituent in C. minima CP was identified as fucoidan and it displayed a higher sulfate content. The degree of sulfation of these polysaccharides suggests a positive correlation with the observed antioxidant properties.

• 인간행동과 음악연구
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• 제18권2호
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• pp.87-107
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• 2021

본 연구의 목적은 연주자가 사물놀이에서 경험하는 악기와의 대상관계 현상을 탐구하는 것이다. 이를 위해 연구자는 사물놀이 연주자 5명을 대상으로 반구조화된 개방형 질문을 통한 심층 면담을 시행하고 자료를 수집하였으며 Giorgi(2004)의 현상학적 연구 방법으로 분석하였다. 연구 결과, 121개의 의미단위, 7개의 하위 범주, 3개의 대범주가 도출되었다. 3개의 대범주는 '중간대상의 매체', '내적 욕구 표현 및 인식의 수단', '타인 인식과 소통의 도구'로 나타났다. 사물놀이에서 악기는 연주자에게 내적 몰입을 도와주는 대상 경험을 하게 하는 매체가 된다. 또한 연주자는 악기연주를 통해 자신의 내적 욕구를 표현하고 자신의 성격과 정체성에 대해 새롭게 인식한다. 이와 동시에 연주자는 악기 특성과 사물놀이에서의 역할에 따라 악기를 사람처럼 대상화하여 내적 표상을 형성하고 이를 중심으로 연주에서의 관계를 이해하고 타연주자와 소통한다. 마지막으로 사물놀이에서 악기는 연주자에게 이상적 자기 성격에 따른 대리적 대상의 역할을 한다. 본 연구의 결과는 사물놀이라는 합주 형태가 연주자의 대상관계 발달을 촉진하며 연주자의 내면화된 표상체계와 상호작용 방식을 외화하고 있음을 알 수 있게 해준다. 본 연구는 연주자와 악기 간의 심리내적인 기능적 관계를 밝히고 음악치료에서 사물놀이 악기 활용에 대한 기초를 제시해주었다는데 의의가 있다.

• Physical Therapy Rehabilitation Science
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• 제8권3호
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• pp.146-151
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• 2019

Objective: The purpose of this study was to investigate the test-retest reliability and concurrent validity of the joint angle of the lower extremities during sit-to-stand movements with wearable sensors based on a portable gait analysis system (PGAS), and the results were compared with a analysis system (MAS) to predict the clinical potential of it. Design: Cross-sectional study. Methods: Sixteen persons with stroke (9 males, 7 females) participated in this study. All subjects had the MAS and designed PGS applied simultaneously and eight sensor units of designed PGAS were placed in a position to avoid overlap with the reflexive markers from MAS. The initial position of the subjects was 90º of hip, knee, and ankle joint flexion while sitting on a chair that was armless and backless. The height of the chair was adjusted to each individual. After each trial, the test administrator checked the quality of data from both systems that measured sit-to-stand for test-retest reliability and concurrent validity. Results: As a result, wearable sensor based designed PGAS and MAS demonstrated reasonable test-retest reliability for the assessment of joint angle in the lower extremities during sit-to-stand performance. The intra-class correlation coefficients (ICCs) for wearable sensor based designed PGAS showed an acceptable test-retest reliability, with ICCs ranging from 0.759 to 0.959. In contrast, the MAS showed good to excellent test-retest reliability, with ICCS ranging from 0.811 to 0.950. In concurrent validity, a significant positive relationship was observed between PGAS and MAS for variation of joint angle during sit-to-stand movements (p<0.01). A moderate to high relationship was found in the affected hip (r=0.665), unaffected hip (r=0.767), affected knee (r=0.876), unaffected knee (r=0.886), affected ankle (r=0.943) and unaffected ankle (r=0.823) respectively. Conclusions: The results of this study indicated that wearable sensor based designed PGAS showed acceptable test-retest reliability and concurrent validity in persons with stroke for sit-to-stand movements and wearable sensors based on developed PGAS may be a useful tool for clinical assessment of functional movement.

• 한국정보통신학회논문지
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• 제26권6호
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• pp.850-858
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• 2022

리소스가 제한된 임베디드 장치에 GRU를 배포하기 위해 이 논문은 구조적 압축을 가능하게 하는 재구성 가능한 FPGA 기반 GRU 가속기를 설계한다. 첫째, 조밀한 GRU 모델은 하이브리드 양자화 방식과 구조화된 top-k 프루닝에 의해 크기가 대폭 감소한다. 둘째, 본 연구에서 제시하는 재사용 컴퓨팅 패턴에 의해 외부 메모리 액세스에 대한 에너지 소비가 크게 감소한다. 마지막으로 가속기는 알고리즘-하드웨어 공동 설계 워크플로의 이점을 얻는 구조화된 희소 GRU 모델을 처리할 수 있다. 또한 모든 차원, 시퀀스 길이 및 레이어 수를 사용하여 GRU 모델에 대한 추론 작업을 유연하게 수행할 수 있다. Intel DE1-SoC FPGA 플랫폼에 구현된 제안된 가속기는 일괄 처리가 없는 구조화된 희소 GRU 네트워크에서 45.01 GOPs를 달성하였다. CPU 및 GPU의 구현과 비교할 때 저비용 FPGA 가속기는 대기 시간에서 각각 57배 및 30배, 에너지 효율성에서 300배 및 23.44배 향상을 달성한다. 따라서 제안된 가속기는 실시간 임베디드 애플리케이션에 대한 초기 연구로서 활용, 향후 더 발전될 수 있는 잠재력을 보여준다.

• 대한영양사협회학술지
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• 제28권2호
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• pp.67-84
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• 2022

This study aimed to explore the status of food service outsourcing behavior of long-term care institutions (LTCIs) through a cross-sectional survey using a questionnaire administered between July 16th and August 7th, 2020. The survey respondents were either dietitians or facility managers, who worked at 731 nursing homes, 477 group homes, and 673 day-care centers. Approximately 25.9% of nursing homes, 11.7% of group homes, and 33.1% of day-care centers used a managed-services company to operate their food service units. The main reason for outsourcing food service by nursing homes was related to the staffing of dietitians and cooks, whereas group homes and day-care centers outsourced food services due to factors relating to meal costs and the cooking process. Almost all the LTCIs entered into private contracts for outsourced food services. Only a few food service contracts included the types of meals, nutrition standards such as protein and calories per meal, and the parameter or ratio of food cost. Of the respondents, 84.5% from nursing homes, 87.5% from group homes, and 87.1% from day-care centers agreed that the quality of outsourced food services of the LTCIs should be regulated. Meals are essential for maintaining the health and functional status of LTCI users. As more LTCIs outsource their food services, we suggest the following: (1) Increasing the minimum dietitian staffing standards for LTCIs as per the Welfare of Senior Citizens Act and requiring at least one dietitian for every nursing home, (2) Making it mandatory to use a standard food service contract template when drafting food service contract, and (3) Developing realistic standards for food service operations considering the size and operation type of the LTCIs.

• 한국소프트웨어감정평가학회 논문지
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• 제16권2호
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• pp.1-8
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• 2020

컴퓨터 프로그램의 표절 또는 복제에 대한 유사도 검출은 구현에 사용된 프로그래밍 언어, 분석 대상 코드의 종류에 따라 각기 다른 감정 방법과 도구가 필요하다. 최근 들어 증가하고 있는 내장형 시스템의 목적 코드에 대한 유사도 감정은 원시 코드와 비교해 더욱 복잡한 과정 및 고도의 기술과 함께 상당한 자원을 요구하고 있다. 본 연구에서는 목적 코드를 대상으로 역 어셈블리 기법의 적용과 같은 역공학 방법을 이용하여, 목적 코드의 어셈블리어 변환을 통한 어셈블리어 레벨에서의 함수 단위 유사도 감정 방법에 관하여 연구하였다. 그리고, 어셈블리어 레벨에서의 유사도 비교를 위해 코드의 구문분석을 통한 명령어 및 오퍼랜드 테이블을 생성하고, 이를 대상으로 유사도를 검출할 수 있는 도구의 설계에 관하여 기술하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.28-28
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• 2022

This paper presents the first genome assemblies of Philippine mangoes that provide valuable reference for varietal improvement and genomic studies on mango and related fruit crops. WE sequenced whole genomes of3 species, Mangifera odorata (Huani), Mangifera altissima (Paho), and Mangifera indica 'Carabao' (Sweet Elena). 'Carabao' is the major export variety of the Philippines; Paho is identified as vulnerable by the IUCN Red List of Threatened Species; Huani has fruit sap acrid which is the primary defense mechanism against insects and birds. We used Falcon, a diploid aware -de novo assembler to assemble SMRT generated long-read sequences. Falcon-unzip was employed to phase the output assembly producing larger contig sets (primary contigs) and shorter contigs corresponding to haplotypes (haplotigs). Assembly statistics were generated by comparing the assembly to a reference genome, Tommy Atkins, using Quality Assessment Tool (QUAST). Moreover, the extent of duplication and completeness of gene content was measured using Benchmarking Universal Single-Copy Orthologs (BUSCO). Draft assemblies with high duplications were processed using Purge Haplotigs and Purge Dups to lessen duplications with minimal impact on genome completeness. De novo assemblies of Huani, Paho and 'Carabao' were then generated with primary contig sizes of 463.64 Mb, 508.95 Mb and 401.51 Mb respectively. These draft assemblies of Huani, Paho and 'Carabao' showed 96.90%, 95.17% and 99.07% complete BUSCOs respectively which is comparable to 'Tommy Atkins' genome (98.6%). Using two mango transcriptome data (pooled RNA-seq from different mango varieties and tissues), 91-96% or 24-30 million reads were successfully mapped back for each generated assembly indicating high degree of completeness. The results obtained demonstrated the highly contiguous, phased, and near complete genome assembly of three Philippine mango species for structural and functional annotation of gene units, especially those with economic importance.

• Journal of Microbiology and Biotechnology
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• 제33권8호
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• pp.1101-1110
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• 2023

Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2-2.2 × 10⁶ colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.

• Human Ecology Research
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• 제61권4호
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• pp.647-661
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• 2023

This study explored the relationship between Generation MZ female consumers' participation in sports activity, consumption of body-exposing sportswear, body-esteem, and appearance management behavior. In-depth interviews were conducted with twenty Generation MZ female consumers who regularly participate in sports activity and consume body-exposing sportswear. Based on transcriptions of in-depth interviews conducted with research subjects, the researcher and two assistant researchers collectively categorized and compared common characteristics found in their responses, categorized according to themes using units of words/terms and paragraphs. The main finding were as follows. Firstly, Generation MZ female consumers participated in various sports activities for an average of approximately one hour, three times per week. The effects of such participation were improvements in physical and psychological health. Secondly, Generation MZ female consumers considered aesthetical, functional, and economical factors when they consumed body-exposing sportswear, regardless of the activity in which they participated. This sportswear served to increase the efficiency of exercise and also played a motivational role. Thirdly, the overall body-esteem of Generation MZ female consumers who participated in sports activity regularly and consumed body-exposing sportswear was high. Fourthly, Generation MZ female consumers who regularly participated in sports activity and consumed body-exposing sportswear were practicing active appearance management behavior in order to show how their body had been shaped by sports activity. In addition, they were pursuing four self-images: "polished image pursue", "self-care image pursue", "natural image pursue", and "trustworthy image pursue".

• Genomics & Informatics
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• 제21권3호
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• pp.40.1-40.11
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• 2023

Microbial community profiling using 16S rRNA amplicon sequencing allows for taxonomic characterization of diverse microorganisms. While amplicon sequence variant (ASV) methods are increasingly favored for their fine-grained resolution of sequence variants, they often discard substantial portions of sequencing reads during quality control, particularly in datasets with large number samples. We present a streamlined pipeline that integrates FastP for read trimming, HmmUFOtu for operational taxonomic units (OTU) clustering, Vsearch for chimera checking, and Kraken2 for taxonomic assignment. To assess the pipeline's performance, we reprocessed two published stool datasets of normal Korean populations: one with 890 and the other with 1,462 independent samples. In the first dataset, HmmUFOtu retained 93.2% of over 104 million read pairs after quality trimming, discarding chimeric or unclassifiable reads, while DADA2, a commonly used ASV method, retained only 44.6% of the reads. Nonetheless, both methods yielded qualitatively similar β-diversity plots. For the second dataset, HmmUFOtu retained 89.2% of read pairs, while DADA2 retained a mere 18.4% of the reads. HmmUFOtu, being a closed-reference clustering method, facilitates merging separately processed datasets, with shared OTUs between the two datasets exhibiting a correlation coefficient of 0.92 in total abundance (log scale). While the first two dimensions of the β-diversity plot exhibited a cohesive mixture of the two datasets, the third dimension revealed the presence of a batch effect. Our comparative evaluation of ASV and OTU methods within this streamlined pipeline provides valuable insights into their performance when processing large-scale microbial 16S rRNA amplicon sequencing data. The strengths of HmmUFOtu and its potential for dataset merging are highlighted.