• 한국식생활문화학회지
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• 제36권1호
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• pp.130-142
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• 2021

This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

• 한국응용과학기술학회지
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• 제32권2호
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• pp.312-319
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• 2015

In this study, n-butanol and ethyl acetate extracts were prepared from raw yam (Dioscorea batatas DECNE.). Their antioxidative potencies were investigated employing various in vitro methods, such as ferrous ion chelating, ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging and nitrite scavenging activity. The n-butanol fraction was assayed to possess stronger antioxidant activity by ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and NO radical scavenging activity. However, ethyl acetate extract was more effective in chelating ferrous ion and scavenging nitrite. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

• The Korean Journal of Physiology and Pharmacology
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• 제10권4호
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• pp.181-186
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• 2006

This study was undertaken to evaluate whether peroxisome proliferator-activated-receptor-gamma $(PPAR-{\gamma})$ agonist-rosiglitazone (ROSI) induces postischemic functional recovery in Langendorf heart model. Hearts isolated from normal rats were subjected to 20 min of normoxia or 25 min zero-flow ischemia followed by 50 min reperfusion. In this acute protocol, ROSI $(20\;{\mu}g/ml)$ administered 10 min before ischemia had no effect on hemodynamic cardiac function, but had protective effect on lipid peroxidation in in vitro experiments. In chronic protocol in which ROSI was given by daily gavage (4 mg/kg) for three consecutive days, ROSI could not prevent the hemodynamic alteration on cardiac performance, but has protective effect on the activity of superoxide dismutase (SOD). There was no significant difference in the contents of reduced glutathione (GSH) and catalase activity between ischemia-reperfusion (IR) and ROSI treated IR hearts. Although ROSI had no effect on hemodynamic factor, it had effect on antioxidant activity. Our results indicate that ROSI provides partial beneficial effects by inhibiting lipid peroxidation and/or recovering normal level of SOD activity in the ischemic reperfused heart.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권1호
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• pp.1-6
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• 2017

In relation to the global increase trend of obesity population, there is a demand for the development of foods having high functional activity by mass-extracting anti-obesity active substances using mulberry product such as leaf and fruit. Therefore, we evaluated the antiobesity efficacy according to varieties by using the mulberry leaves and fruit extracts. At the same time, high active varieties were selected. For this purpose, the effects of the extracts of the mulberry leaf and fruit on 3T3-L1 adipocyte differentiation were examined. As a result, in the case of mulberry leaves, the lipid accumulation inhibitory rate of 'Cheongolppong' was higher than that of the control at $500{\mu}g/ml$ treatment. And in the case of the extract of mulberry fruit, 'Daesim' showed the highest lipid accumulation inhibitory rate compared with the control at 50 times of diluted extract.

• 한국자원식물학회지
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• 제20권6호
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• pp.544-547
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• 2007

The effects of hwangryun(Coptidis Rhizoma) on lowering lipid and oxidative stress in the induced obesity rat was observed. The concentration of plasma triglyceride in hwangryun treatment groups showed the low values compared to the control group, and as the increased hwangryun, the concentration of triglyceride decreased. The concentration of plasma total cholesterol decreased in all hwangryun treatment groups. However the concentration of plasma HDL-cholesterol and LDL-cholesterol showed no significantly difference in all the treatment groups. Thiobarbituric acid reactive substance(TBARS) concentration in plasma and liver showed low values in all hwangryun treatment groups compared to the control group. Glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) activities showed no significantly difference in all the treatment groups. However catalase(CAT) activity showed a tendence to increase in hwangryun groups, and in 200mg/kg hwangryun treatment group showed significantly a high value than the control group. Summarizing the results above, hwangryun has the functional materials that lowering lipid and works with oxidative stress.

• Journal of Plant Biology
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• 제36권1호
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• pp.97-104
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• 1993

The lipid composition of thylakoid membranes was compared between mesophyll and bundle sheath chloroplasts of maize. According to mild-denaturing gel electrophoresis, mesophyll thylakoids contained both PS I complex and PS II light-harvesting chlorophyll-protein complex(LHCP), while those of bundle sheath cells contained mainly PS I complex. The amount of lipids per mg chlorophyll was higher in bundle sheath thylakoids than in mesophyll. The major polar lipid classes were monogalactosyldiacylglycerol(MGDG), digalactosyldiacylglycreol, sulfolipid and phosphatidylglycerol (PG) in both tissues. Linolenic acid(18 : 3), linoleic acid(18 : 2) and palmitic acid(16 : 0) were the main fatty acyl components, with higher ratio of unsaturated to saturated fatty acids in bundle sheath thylakoids, suggesting these membranes are more fluid. The most striking difference in lipid composition between the two kinds of tissues was the practical absence of trans- 3-hexadecenoic acid(16 : 1t) in PG of bundle sheath thylakoids. This fatty acid is known to be involved in the association of LHCP as oligomeric form. More than 80% of MGDG molecular species was 18 : 3, 18 : 3, demonstrating that maize is a typical 18 : 3 plant. Therefore, the possibility of the functional relationships between the lamella structure, and thus the distribution of photosystems, and MGDG molecular species was excluded.

• 한국식품영양과학회지
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• 제34권1호
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• pp.42-56
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• 2005

본 연구는 건강검진센터 검진자 중 혈청 중성지방 농도가 150 mg/dL 이상이거나 총 콜레스테롤이 200 mg/dL 이상인 고지혈증 초기 판정자 43명을 대상으로 12주 동안 의학영양치료와 병행하여 한방 기능성 차를 섭취하게 한 후 혈청 지질농도와 항산화에 미치는 영향을 조사한 것이다. 그 결과를 요약하면 다음과 같다. 총 대상자 43명의 평균 연령은 49.41$\pm$5.2세였으며, 남성의 비율이 53.5%였다. 대상자의 51.2%가 혈중 콜레스테를 수치만 높은 제 II a형이었으며, 대상자의 37.2%는 혈중 중성지방과 콜레스테롤이 함께 높은 제IIb형, 11.6%는 중성지방만 높은 제 IV형이었다 모든 대상자는 2003년 건강검진결과 처음으로 고지혈증을 진단받았으며, 지질저하제를 복용하지 않았다. 또한 모든 대상자는 의학영양치료를 받아본 경험이 없었다. 체중은 남여 대상자 모두 군간의 차이가 없었으며, 실험 전과 후의 변화가 관찰되지 않았다. 남성의 경우, 허리둘레가 HFT군에서 유의적으로 감소하였으며, 혈압 또한 HFT군에서 유의적으로 감소하였다. 여성의 경우는 BMI가 FFT군에서 실험 후 유의적으로 감소하였으며, 체지방 비율은 모든 군에서 감소하였다. 식사량, 식사의 규칙성, 식품군의 배합 및 짠 음식 에 대한 기호도 등을 조사한 결과 실험 전 식사량은 만복이 될 때까지 섭취하는 경우가 많았고, 사 시 배합에는 별로 관심이 없었으며, 평균 주 2∼3회의 식사를 거르고 있는 것으로 나타났다. 그러나 실험 후의 이와 같은 양상은 많은 변화를 보였다. 기능성차 섭취와 의학영양치료 전후의 식품 섭취 빈도는 실험 전 콜레스테롤이 많은 음식, 간식의 빈도가 실험 후 유의적으로 감소하였다. 모든 군에서 평균 섭취 열량이 실험 전보다 유의적으로 감소하였다. 총 지방의 섭취량도 유의적으로 섭취 감소를 보였으며, 포화지방산의 경우 HFT군에서 가장 큰 감소를 보였다. 다가 불포화지방산 또한 HFT군에서 감소하였다. 대상자의 P/M/S섭취 비율은 실험 전 0.73/1.l1/1에서 실험 후 0.98/1.38/l로 다가 불포화지방산의 섭취량과 단일 불포화지방산의 섭취 비율이 증가한 것으로 나타났다. 12주간의 식이 콜레스테롤 섭취량도 모든 군에서 매우 큰 감소를 보였다. 혈중 총 콜레스테롤 농도를 비교한 결과 기능성차군에서 유의적으로 감소하였다, 중성지방 농도 또한 총 콜레스테롤 농도와 마찬가지로 기능성차군에서 유의적으로 감소하였다. 혈중 LDL-콜레스테롤과 HDL-콜레스테롤 농도는 실험 전과 후의 유의적인 변화는 없었다, 고지혈증의 발병율과 밀접한 상관관계를 보이는 동맥경화지수는 기능성차군에서 유의적으로 감소하였다. 혈중 호모시스테인 농도의 측정 결과 FFT군의 경우 실험 전 정상범위를 초과하였으나 실험 후 정상 범위에 속하게 되었다. 항산화 물질 2항목(SOD, GPx) 측정결과 SOD는 실험 후 HFT군에서 유의적으로 증가하였다.(13.3%). GPx는 실험 후 FFT군에서 유의적으로 증가되었다.(12.8%). 결론적으로 의학영양치료는 식습관 및 식품섭취의 패턴을 변화시켰고, 총열량과 동물성 단백질, 지방질, 콜레스테롤 섭취의 저하를 유도하였으나 혈중 지질 농도에는 유의적인 변화를 나타내지 못하였다. 그러나 대상자 개인에 맞는 의학영양치료의 적용은 약물요법 이전에 식습관과 섭취량을 조절하는데 매우 유효할 것으로 사료된다. 한편 의학영양치료와 병행한 기능성차(상엽, 구기자, 국화, 대추, 참깨, 나복자가 복합 처방)의 섭취는 혈중 지질 농도를 저하시켰으며, 또한 항산화 효과도 나타내었다. 이와 같이 임상 효능이 확인된 한방재료로 제조된 기능성차의 섭취는 동맥경화증, 허혈성 심장질환, 뇌졸중 등 심혈관계 질환의 주요 원인인 고지혈증에 효과가 있는 것으로 나타났으며, 또한 혈중 지질 농도를 저하시킴으로써 질환을 예방하는데 의학영양치료의 보조적인 역학을 할 수 있는 기능성차로 차별화 될 수 있을 것으로 사료된다.

• 한국식생활문화학회지
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• 제20권3호
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• pp.293-298
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• 2005

This study was investigated to examine the physicochemical properties of functional rice and the adaptable food processing of the added functional rice. The functional rice are Sanghwang rice, Agaricus rice(fermented with basidiomycota), Tochukaso rice, fermented with Monascus rubber rice. Fermented with Monascus rubber rice contains more crude protein, curde lipid, moisture, and total dietary fiber. The cross sectional shapes of the functional rice show polygon shapes, the starch granules of those rice are transformed. In sensory evaluation, fermented with Monascus rubber rice was showed the lowest values for glossness, color, flavor, sweetness, harshness, overall acceptability. The textural properties for those ratios were also affected by the period of storage. After 48 hours, the lowest hardness level was 30% Tochukaso rice addition, and the highest hardness level was 10% Sangwhang rice addition case. In 30% Sangwhang rice, and 30% tochukaso rice case gumminess values were decreased after 48 hours. But in 30% Sangwhang rice, and 30% Tochukaso rice case, the chewiness values decreased. This result suggest that the most suitable addition ratio for the rice cooking condition is 10% functional rice addition.

• 한국수산과학회지
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• 제49권1호
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• pp.30-37
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• 2016

Microalgae are functional foods because they contain special anti-aging inhibitors and other functional components, such as ecosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and omega-3 polyunsaturated fatty acids. Many of these functional dietary components are absent in animals and terrestrial plants. Thus, microalgae are widely utilized in human functional foods and in the feed provided to farmed fish and terrestrial livestock. Many marine organisms consume microalgae, often because they are in an appropriate portion of the cell size spectrum, but also because of their nutritional content. The nutritional requirements of marine organisms differ from those of terrestrial animals. After hatching, marine animals need small live forage species that have high omega-3 polyunsaturated fatty acid contents, including EPA and DHA. Euglena cells have both plant and animal characteristics; they are motile, elliptical in shape, 15-500 μm in diameter, and have a valuable nutritional content. Mixotrophic cell cultivation provided the best growth rates and nutritional content. Diverse carbon (fructose, lactose, glucose, maltose and sucrose) and nitrogen (tryptone, peptone, yeast extract, urea and sodium glutamate) supported the growth of microalgae with high lipid contents. We found that the best carbon and nitrogen sources for the production of high quality Euglena cells were glucose (10 g L^–1) and sodium glutamate (1.0 g L^–1), respectively.

• 생명과학회지
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• 제7권4호
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• pp.361-370
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• 1997

Sprague-Dawley male rats were fed experimental diet, and also were orally daministerred with 3.0% ultra low viscosity (ULV) sodium alginate-added functional drink(AL-3.0% group : HAEJOMIN), 5.0% polyedxtrose(PD)-added drink(PD-5.0group) and 2.5% polydextrose-added drink(PD-2.5 group) for 8 weeks. Effect of rhese dietary fiber-added functional drinks on body weight, feed and gross efficincies, triglyceride and cholesterol levels, LDL- cholesterol levels, hydroxyl radical and malondialdehyde levels, and superoxide dismutase (SOD) activity in serum of SD rats were evaluated. Administration of AL-3.0 drink and PD-5.0 drink resulted in a marked inhibition in increase of body weight compared with control and PD-2.5 groups for 8 weeks. Inhibition effect in body weight in 3.0% alginic acid-added drink )AL-3.0 froup_ showed a same trend in 5.0% polydextrose(PD)-added drink (PD-5.0 group)(p<0.001). Therefore, it is found that inhibition effects of obesity in 3.0% alginic acid-added drink were higher 2 times than that in same concentration of polydextrose(PD)-added drink. Triglyceride and cholesterol levels in AL-3.0 and PD-5.0 groups significantly decreased to 25$\sim$30% compared with control group(p<0.01$\sim$0.001), but there were no significant differences in these drinks. LDL-cholesterol levels in AL-3.0 group significantly decreased about 15% compared with PD-5.0 group, but atherogenic index in AL-3, 0 group showed a similar trend to that in PD-5.0 group. Hydroxyl radical formations and lipid peroxide(LPO) levels in AL-3, 0 and PD-5.0 groups significantly decreased to 15% and 20%, respectively, compared with control group(p, 0.05$\sim$0.01), but there were no significant differences in these drinks. Superoxide, dismutase(SOD) activity in AL-3.0 group significantly higher (about 255) than those in control and PD-5.0 groups(p<0.01). These results suggest that administration of ULV-sodium alginate-added functional drink(HAEJOMIIN) effectively can not only inhibit obesity, but also can intervent chronic degenerative disease and aging process.