• BMB Reports
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• v.40 no.3
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• pp.349-357
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• 2007

The ubiquitous family of 14-3-3 proteins functions as regulators in a variety of physiological processes. Eight rice 14-3-3 genes, designated OsGF14a through h, were identified from an exhaustive search of the genome database. Comparisons of deduced amino acid sequences reveal a high degree of identity among members of the OsGF14 family and reported Arabidopsis 14-3-3 proteins. A phylogenetic study indicates that OsGF14s contain both $\varepsilon$ and non-$\varepsilon$ forms, which is also confirmed by a structural analysis of OsGF14 genes. Furthermore, transcripts of OsGF14b, OsGF14c, OsGF14d, OsGF14e, OsGF14f and OsGF14g were detected in rice tissues. Their different expression patterns, the different effects of environmental stresses and plant hormones on their transcription levels, and the different complementary phenotypes in yeast 14-3-3 mutants not only indicates that OsGF14s are responsive to various stress conditions and regulated by multiple signaling pathways, but also suggests that functional similarity and diversity coexist among the members of OsGF14 family.

• Molecules and Cells
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• v.38 no.4
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• pp.304-311
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• 2015

Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

• The International Journal of Costume Culture
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• v.12 no.1
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• pp.38-51
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• 2009

The goal of this study is to define the types of the lifestyles of senior consumers and identify the differences in the properties of apparel products and the apparel attitudes. To collect the data for this study, questionnaires for the research were distributed from November 20, 2006 to December 15, 2006 to those over 50 living in Seoul, Pusanand Kyunggi and 302 questionnaires were used for the data analysis. The results of the study are as follows. First, six factors were extracted which were "Pursuit of Self-development", "Pursuit of Active Life", "Pursuit of Material", "Pursuit of Diversity", "Pursuit of Family-oriented" and "Pursuit of Recreational Life" after factor analysis of lifestyles recognized by the senior consumers that participated in this study. Second, the lifestyles of the senior consumers were categorized into "Consumption-oriented Type", "Personal Satisfaction-oriented Type", "Family Weighted Type" and "Recreation-oriented Type." Third, three factors were extracted which were "Symbolical Property", "Functional Property" and "Customer Support Property" after conducting the factor analysis on the properties of apparel products. Fourth, significant differences were shown in apparel properties by the lifestyle types of senior consumers in the symbolical property and the customer support property. The "symbolical property" was shown highest in "recreation-oriented type" and lowest in the "family weighted type." The customer support property was shown highest in the "family weighted type" and lowest in the "recreation-oriented type", showing the opposite result. Fifth, significant differences were shown in apparel attitudes by the lifestyle types of senior consumers in "Fashion Innovativeness", "Apparel Involvement" and "Apparel Necessity." The "fashion innovativeness" was shown highest in the "recreation-oriented type" and lowest in the "family weighted type." The apparel involvement and the necessity for apparel for senior citizens was shown high in the "recreation-oriented type" and this showed that the senior consumers valuing recreation also value fashion, have high apparel involvement and feel the necessity for apparel for senior citizens.

• Genomics & Informatics
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• v.21 no.2
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• pp.23.1-23.9
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• 2023

The mammalian sirtuin family, consisting of SIRT1-SIRT7, plays a vital role in various biological processes, including cancer, diabetes, neurodegeneration, cardiovascular disease, cellular metabolism, and cellular homeostasis maintenance. Due to their involvement in these biological processes, modulating sirtuin activity seems promising to impact immuneand aging-related diseases, as well as cancer pathways. However, more understanding is required regarding the safety and efficacy of sirtuin-targeted therapies due to the complex regulatory mechanisms that govern their activity, particularly in the context of multiple targets. In this study, the interaction landscape of the sirtuin family was analyzed using a systems biology approach. A sirtuin protein-protein interaction network was built using the Cytoscape platform and analyzed using the NetworkAnalyzer and stringApp plugins. The result revealed the sirtuin family's association with numerous proteins that play diverse roles, suggesting a complex interplay between sirtuins and other proteins. Based on network topological and functional analysis, SIRT1 was identified as the most prominent among sirtuin family members, demonstrating that 25 of its protein partners are involved in cancer, 22 in innate immune response, and 29 in aging, with some being linked to a combination of two or more pathways. This study lays the foundation for the development of novel therapies that can target sirtuins with precision and efficacy. By illustrating the various interactions among the proteins in the sirtuin family, we have revealed the multifaceted roles of SIRT1 and provided a framework for their possible roles to be precisely understood, manipulated, and translated into therapeutics in the future.

• Natural Product Sciences
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• v.10 no.2
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• pp.89-91
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• 2004

Six components were isolated from the $CH_2Cl_2$ fraction of Belamcanda chinensis rhizome by open column chromatography. Their structures were elucidated as ${\beta}-sitosterol$ (1), apocynin (2), decursin (3), iristectorigenin A (4), irigenin (5) and tectorigenin (6) by spectral analysis. Among these compounds, decursin (3) was isolated for the first time from a plant of the family Iridaceae.

• Journal of the Korean Magnetic Resonance Society
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• v.11 no.2
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• pp.110-114
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• 2007

[ $^{31}P$ ] powder pattern spectra were measured to investigate the aspects of the interaction between the MLV (Multilamellar vesicle) and PMAP-23, a membrane of cathelicidin family and then CSAs(chemical shift anisotropy) were calculated to indentify the extent of perturbation of phospholipid mobility by the peptides. We found that acidic phospholipid interacts strongly with PMAP-23, and the analogues which modified to increase the amphipathic property showed that larger change of CSA. The analogue which introduced positive charge showed the same effects with amphipathic property.

• BMB Reports
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• v.38 no.5
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• pp.619-623
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• 2005

The efficacy of chemotherapeutic agents on tumor cells has been shown to be modulated by tumor suppressor gene p53 and its target genes such as Bcl-2 family members (Bax, Noxa, and PUMA). However, various chemotherapeutic agents can induce cell death in tumor cells that do not express the functional p53, suggesting that some chemotherapeutic agents may induce cell death in a p53-independent pathway. Here we showed that etoposide can induce the similar degree of cell death in p53-deficient HCT 116 cells, whereas 5'-FU-mediated cell death is strongly dependent on the existence of functional p53 in HCT 116 cells. Further, we provide the evidence that etoposide can induce the cytochrome c release from isolated mitochondria, and etoposide-induced cytochrome c release is not accompanied with the large amplitude swelling of mitochondria. These data suggest that etoposide can directly induce the mitochondrial dysfunction irrespective of p53 status, and it may, at least in part, account for the p53-independent pathway in cell death induced by chemotherapeutic agents.

• Advances in Traditional Medicine
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• v.3 no.4
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• pp.163-179
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• 2003

The genus Polygonatum species belongs to the family Liliaceae which is widely distributed over areas of the north temperature zone. There are about forty more plants of Polygonatum species in the world widely distributed in eastern Europe and south east Asia. The plants of Polygonatum species have been used not only as ornamental plants but also for their medicinal values. This article is concerned with the specific properties and flavour of the drug and its history as a medicine, showing the main functional components of Polygonatum species of flavonoids, steroidal glycosides, and saccharides.

• Communications of the Korean Mathematical Society
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• v.9 no.3
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• pp.547-564
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• 1994

Let X be a real Banach space. We consider the existence of solutions of the abstract nonlinear functional evolution equation : $$ (E) \frac{du(t)}{dt} + A(t)u(t) + F(u)(t) \ni h(t), $$ $$ u(s) = x_o \in D(A(s)), 0 \leq s \leq t \leq T, $$ where u : $[s, T] \to x$ is an unknown function, ${A(t) : 0 \leq t \leq T}$ is a given family of nonlinear (possibly multivalued) operators in X, and $F : C([s, t];X) \to L^{\infty}([s, X];X)$ and $h : [s, T] \to X$ are given functions.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.270.2-270.2
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• 2002

Dopamine D2 and D3 receptors (D2R and D3R) belong to pharmacological D2R family and share similar structural and functional characteristics. Elucidation of their differential functional characteristics is important for understanding their roles in brain. ERK1/2 was chosen as an example of signaling component of D2R and D3R and systemic studies were conducted to understand the regulatory mechanisms on ERK1/2 activation. (omitted)